Angel Orte

Summary

Affiliation: University of Granada
Country: Spain

Publications

  1. doi request reprint Intracellular Zn(2+) detection with quantum dot-based FLIM nanosensors
    Consuelo Ripoll
    Department of Physical Chemistry, Faculty of Pharmacy, University of Granada, Campus Cartuja, 18071 Granada, Spain
    Chem Commun (Camb) 51:16964-7. 2015
  2. doi request reprint Single-molecule fluorescence coincidence spectroscopy and its application to resonance energy transfer
    Angel Orte
    Department of Physical Chemistry, Faculty of Pharmacy, University of Granada, Campus Cartuja, 18071, Granada, Spain
    Chemphyschem 12:491-9. 2011
  3. doi request reprint Similarity between the kinetic parameters of the buffer-mediated proton exchange reaction of a xanthenic derivative in its ground- and excited-state
    Jose M Paredes
    Department of Physical Chemistry, University of Granada, Cartuja Campus, Granada, 18071, Spain
    Phys Chem Chem Phys 12:323-7. 2010
  4. doi request reprint A chloride ion nanosensor for time-resolved fluorimetry and fluorescence lifetime imaging
    Maria J Ruedas-Rama
    Department of Physical Chemistry, Faculty of Pharmacy, University of Granada, Campus Cartuja, 18071, Granada, Spain
    Analyst 137:1500-8. 2012
  5. ncbi request reprint Bulk and single-molecule fluorescence studies of the saturation of the DNA double helix using YOYO-3 intercalator dye
    Sergio G Lopez
    Department of Physical Chemistry, Faculty of Pharmacy, University of Granada, Cartuja Campus, 18071, Granada, Spain
    J Phys Chem B 116:11561-9. 2012
  6. doi request reprint Photophysics and binding constant determination of the homodimeric dye BOBO-3 and DNA oligonucleotides
    Maria J Ruedas-Rama
    Department of Physical Chemistry, Faculty of Pharmacy, University of Granada, Campus Cartuja, 18071, Granada, Spain
    J Phys Chem B 114:1094-103. 2010
  7. ncbi request reprint Absorption and emission study of 2',7'-difluorofluorescein and its excited-state buffer-mediated proton exchange reactions
    Angel Orte
    Department of Physical Chemistry, University of Granada, Cartuja Campus, Granada 18071, Spain
    J Phys Chem A 109:734-47. 2005
  8. doi request reprint Binding of BOBO-3 intercalative dye to DNA homo-oligonucleotides with different base compositions
    Maria J Ruedas-Rama
    Department of Physical Chemistry, Faculty of Pharmacy, University of Granada, Campus Cartuja, 18071 Granada, Spain
    J Phys Chem B 114:6713-21. 2010
  9. doi request reprint Real-time phosphate sensing in living cells using fluorescence lifetime imaging microscopy (FLIM)
    Jose M Paredes
    Department of Physical Chemistry, University of Granada
    J Phys Chem B 117:8143-9. 2013
  10. doi request reprint Tuned lifetime, at the ensemble and single molecule level, of a xanthenic fluorescent dye by means of a buffer-mediated excited-state proton exchange reaction
    Jose M Paredes
    Department of Physical Chemistry, University of Granada, Cartuja Campus, 18071 Granada, Spain
    Phys Chem Chem Phys 11:5400-7. 2009

Collaborators

Detail Information

Publications27

  1. doi request reprint Intracellular Zn(2+) detection with quantum dot-based FLIM nanosensors
    Consuelo Ripoll
    Department of Physical Chemistry, Faculty of Pharmacy, University of Granada, Campus Cartuja, 18071 Granada, Spain
    Chem Commun (Camb) 51:16964-7. 2015
    ..The sensing mechanism was based on photoinduced electron transfer (PET) between an azacycle receptor group and the QD nanoparticles. ..
  2. doi request reprint Single-molecule fluorescence coincidence spectroscopy and its application to resonance energy transfer
    Angel Orte
    Department of Physical Chemistry, Faculty of Pharmacy, University of Granada, Campus Cartuja, 18071, Granada, Spain
    Chemphyschem 12:491-9. 2011
    ..We highlight the advantages of the coincidence spectroscopy and illustrate this with examples of its application to some biological systems of interest...
  3. doi request reprint Similarity between the kinetic parameters of the buffer-mediated proton exchange reaction of a xanthenic derivative in its ground- and excited-state
    Jose M Paredes
    Department of Physical Chemistry, University of Granada, Cartuja Campus, Granada, 18071, Spain
    Phys Chem Chem Phys 12:323-7. 2010
    ..The rate constant obtained supported the uniformity of the process in the ground and the excited state, and the need of adequate character of the buffer species to be able to promote excited-state reactions...
  4. doi request reprint A chloride ion nanosensor for time-resolved fluorimetry and fluorescence lifetime imaging
    Maria J Ruedas-Rama
    Department of Physical Chemistry, Faculty of Pharmacy, University of Granada, Campus Cartuja, 18071, Granada, Spain
    Analyst 137:1500-8. 2012
    ..These results show the promising application of the QD-lucigenin nanosensors in FLIM, particularly for intracellular sensing, with the invaluable advantages of the time-resolved fluorescence techniques...
  5. ncbi request reprint Bulk and single-molecule fluorescence studies of the saturation of the DNA double helix using YOYO-3 intercalator dye
    Sergio G Lopez
    Department of Physical Chemistry, Faculty of Pharmacy, University of Granada, Cartuja Campus, 18071, Granada, Spain
    J Phys Chem B 116:11561-9. 2012
    ..The spectral features of the aggregate suggest that it may have the structure of a parallel H-aggregate...
  6. doi request reprint Photophysics and binding constant determination of the homodimeric dye BOBO-3 and DNA oligonucleotides
    Maria J Ruedas-Rama
    Department of Physical Chemistry, Faculty of Pharmacy, University of Granada, Campus Cartuja, 18071, Granada, Spain
    J Phys Chem B 114:1094-103. 2010
    ..8 +/- 1.1) x 10(5) and (2.6 +/- 0.3) x 10(5) M(-1), respectively. The corresponding recovered number of base pairs covered were 5.9 +/- 0.2 and 3.5 +/- 0.5 sites for each mode...
  7. ncbi request reprint Absorption and emission study of 2',7'-difluorofluorescein and its excited-state buffer-mediated proton exchange reactions
    Angel Orte
    Department of Physical Chemistry, University of Granada, Cartuja Campus, Granada 18071, Spain
    J Phys Chem A 109:734-47. 2005
    ..Steady-state emission spectra of 2',7'-difluorofluorescein in aqueous solution are also described, where the strong photoacid behavior of the cation is noteworthy...
  8. doi request reprint Binding of BOBO-3 intercalative dye to DNA homo-oligonucleotides with different base compositions
    Maria J Ruedas-Rama
    Department of Physical Chemistry, Faculty of Pharmacy, University of Granada, Campus Cartuja, 18071 Granada, Spain
    J Phys Chem B 114:6713-21. 2010
    ..2 +/- 1.1 sites and has a lower affinity. These differences in the binding features and spectral properties of BOBO-3 may be used to develop approaches to identify GC- or AT-rich regions within large strands of dsDNA...
  9. doi request reprint Real-time phosphate sensing in living cells using fluorescence lifetime imaging microscopy (FLIM)
    Jose M Paredes
    Department of Physical Chemistry, University of Granada
    J Phys Chem B 117:8143-9. 2013
    ..These changes were consistent with increased alkaline phosphatase activity in the extracellular medium as a marker of the differentiation process. ..
  10. doi request reprint Tuned lifetime, at the ensemble and single molecule level, of a xanthenic fluorescent dye by means of a buffer-mediated excited-state proton exchange reaction
    Jose M Paredes
    Department of Physical Chemistry, University of Granada, Cartuja Campus, 18071 Granada, Spain
    Phys Chem Chem Phys 11:5400-7. 2009
    ..The tuned lifetimes obtained by means of phosphate concentration at constant pH have also been recovered at the single-molecule level...
  11. doi request reprint Fluorescence lifetime imaging microscopy for the detection of intracellular pH with quantum dot nanosensors
    Angel Orte
    Department of Physical Chemistry, Faculty of Pharmacy, University of Granada, Campus Cartuja, 18071, Granada, Spain
    ACS Nano 7:6387-95. 2013
    ..A detailed description of the advantages of the FLIM technique is presented. ..
  12. doi request reprint Photophysics of the interaction between a fluorescein derivative and Ficoll
    Jose M Paredes
    Department of Physical Chemistry, University of Granada, Granada, Spain
    J Phys Chem A 115:13242-50. 2011
    ..Therefore, our overall data clearly demonstrate that Ficoll is not an inert crowding agent when in the presence of fluorescein derivative dyes...
  13. doi request reprint Effect of surface modification on semiconductor nanocrystal fluorescence lifetime
    Maria J Ruedas-Rama
    Department of Physical Chemistry, Faculty of Pharmacy, University of Granada, Campus Cartuja, 18071, Granada, Spain
    Chemphyschem 12:919-29. 2011
    ..The results obtained demonstrate that time-resolved fluorescence is a useful tool for QD-based sensing to set the basis for the development of time-resolved-based nanosensors...
  14. doi request reprint Influence of the solvent on the ground- and excited-state buffer-mediated proton-transfer reactions of a xanthenic dye
    Jose M Paredes
    Department of Physical Chemistry, University of Granada, Cartuja Campus, 18071 Granada, Spain
    Phys Chem Chem Phys 13:1685-94. 2011
    ..The proton-transfer rate constants recovered here show a trend correlated with the Hofmeister series or the Marcus classification of ions...
  15. doi request reprint Dynamics of water-in-oil nanoemulsions revealed by fluorescence lifetime correlation spectroscopy
    Angel Orte
    Department of Physical Chemistry, Faculty of Pharmacy, Cartuja Campus, Granada University, 18071 Granada, Spain
    Langmuir 27:12792-9. 2011
    ..The FLCS curves show dynamics in the microsecond time range, which represents an interconversion rate that changes the distribution of the TG-II neutral and anionic forms in the hydrophobic interface and the water core...
  16. doi request reprint Formation of stable BOBO-3 H-aggregate complexes hinders DNA hybridization
    Maria J Ruedas-Rama
    Department of Physical Chemistry, Faculty of Pharmacy, University of Granada, Campus Cartuja, 18071 Granada, Spain
    J Phys Chem B 114:9063-71. 2010
    ..To the best of our knowledge, this is the first time such a stable H-aggregate of a dimeric cyanine dye facilitated by specific nucleotide bases in single strands has been reported...
  17. ncbi request reprint Three-state 2',7'-difluorofluorescein excited-state proton transfer reactions in moderately acidic and very acidic media
    Angel Orte
    Department of Physical Chemistry, University of Granada, Cartuja Campus, 18071 Granada, Spain
    J Phys Chem A 109:8705-18. 2005
    ....
  18. doi request reprint Single-molecule two-colour coincidence detection to probe biomolecular associations
    Angel Orte
    Department of Physical Chemistry, Faculty of Pharmacy, Campus Cartuja, Granada 18071, Spain
    Biochem Soc Trans 38:914-8. 2010
    ....
  19. doi request reprint Photophysics of a Live-Cell-Marker, Red Silicon-Substituted Xanthene Dye
    Luis Crovetto
    Department of Physical Chemistry, Faculty of Pharmacy, University of Granada, Cartuja Campus, 18071 Granada, Spain
    J Phys Chem A 119:10854-62. 2015
    ..Finally, we determined whether 2-Me TM could be used as a sensor inside live cells by measuring the intensity profile of the probe in different cellular compartments of HeLa 229 cells. ..
  20. ncbi request reprint 2',7'-Difluorofluorescein excited-state proton reactions: correlation between time-resolved emission and steady-state fluorescence intensity
    Angel Orte
    Department of Physical Chemistry, University of Granada, Cartuja Campus, 18071 Granada, Spain
    J Phys Chem A 109:2840-6. 2005
    ..The agreement between the experimental and simulated data is excellent...
  21. doi request reprint Fluorescent nanoparticles for intracellular sensing: a review
    Maria J Ruedas-Rama
    Department of Physical Chemistry, Faculty of Pharmacy, University of Granada, Granada, Spain
    Anal Chim Acta 751:1-23. 2012
    ..Finally, we also give an overview of the current methods for delivering of fluorescent NPs into cells, where critically examine the benefits and liabilities of each strategy...
  22. ncbi request reprint Effects of the anion salt nature on the rate constants of the aqueous proton exchange reactions
    Jose M Paredes
    Department of Physical Chemistry, University of Granada, Cartuja Campus, 18071 Granada, Spain
    Phys Chem Chem Phys 14:5795-800. 2012
    ....
  23. doi request reprint Quantum dot photoluminescence lifetime-based pH nanosensor
    Maria J Ruedas-Rama
    Department of Physical Chemistry, Faculty of Pharmacy, University of Granada, Campus Cartuja, 18071, Granada, Spain
    Chem Commun (Camb) 47:2898-900. 2011
    ..2-6.9. These nanosensors have been satisfactorily applied for pH estimation in simulated intracellular media, with high sensitivity and high selectivity toward most of the intracellular components...
  24. doi request reprint The First Step of Amyloidogenic Aggregation
    Fabio Castello
    Department of Physical Chemistry, Faculty of Pharmacy, University of Granada, Campus Cartuja, 18071 Granada, Spain
    J Phys Chem B 119:8260-7. 2015
    ..Furthermore, these experiments have allowed us to obtain quantitative information regarding the oligomer stability. These pre-amyloidogenic oligomers may potentially serve as the first target for fibrillization-prevention strategies. ..
  25. doi request reprint Interaction of YOYO-3 with different DNA templates to form H-aggregates
    Maria J Ruedas-Rama
    Department of Physical Chemistry, Faculty of Pharmacy, University of Granada, Cartuja Campus, 18071, Granada, Spain
    J Phys Chem B 118:6098-106. 2014
    ..As the YOYO-3 H-aggregates are nonfluorescent, our results provide an important basis to quantitatively understand the fluorescence emission of this cyanine dye in the presence of DNA strands. ..
  26. pmc Early amyloidogenic oligomerization studied through fluorescence lifetime correlation spectroscopy
    Jose M Paredes
    Department of Physical Chemistry, Faculty of Pharmacy, Campus Cartuja, Granada, 18071, Spain E Mails J M P M J R R F C
    Int J Mol Sci 13:9400-18. 2012
    ....
  27. ncbi request reprint Fluorescence energy transfer between fluorescein label and DNA intercalators to detect nucleic acids hybridization in homogeneous media
    Eva M Talavera
    Department of Physical Chemistry, Cartuja Campus, Granada University, 18071 Granada, Spain
    Appl Spectrosc 57:208-15. 2003
    ..Moreover, the site label is not critical and can be labeled randomly in the DNA strand. Thus, the method is simpler than those published previously based on FRET. The experiments were carried out in both direct and competitive formats...