Puri Fortes

Summary

Affiliation: University of Navarra
Country: Spain

Publications

  1. pmc Requirements for gene silencing mediated by U1 snRNA binding to a target sequence
    Xabi Abad
    Division of Hepatology and Gene Therapy, CIMA UNAV Pio XII, 55, 31008 Pamplona, Spain
    Nucleic Acids Res 36:2338-52. 2008
  2. pmc Increased in vivo inhibition of gene expression by combining RNA interference and U1 inhibition
    Lorea Blázquez
    Department of Hepatology and Gene Therapy, Center for Applied Medical Research CIMA, University of Navarra, Pio XII 55 31008 Pamplona, Spain
    Nucleic Acids Res 40:e8. 2012
  3. pmc Adenovirus VA RNA-derived miRNAs target cellular genes involved in cell growth, gene expression and DNA repair
    Oscar Aparicio
    Digna Biotech and Bioinformatics Unit, CIMA, University of Navarra, Pio XII 55, 31008, Pamplona, Spain
    Nucleic Acids Res 38:750-63. 2010
  4. pmc Combination of RNA interference and U1 inhibition leads to increased inhibition of gene expression
    X Abad
    Department of Hepatology and Gene Therapy, Center for Applied Medical Research CIMA, University of Navarra, Pamplona 31008, Spain
    Nucleic Acids Res 38:e136. 2010
  5. ncbi request reprint U1 snRNP control of 3'-end processing and the therapeutic application of U1 inhibition combined with RNA interference
    L Blazquez
    Department of Hepatology and Gene Therapy, Center for Applied Medical Research CIMA, University of Navarra, Pamplona, Spain
    Curr Mol Med 13:1203-16. 2013
  6. ncbi request reprint Identification and characterization of RED120: a conserved PWI domain protein with links to splicing and 3'-end formation
    Puri Fortes
    Division of Gene Therapy and Hepatology, CIMA, University of Navarra, Pamplona, Spain
    FEBS Lett 581:3087-97. 2007
  7. pmc Inhibiting expression of specific genes in mammalian cells with 5' end-mutated U1 small nuclear RNAs targeted to terminal exons of pre-mRNA
    Puri Fortes
    Department of Medicine, University of Navarra, Irunlarrea 1, Pamplona 31008, Spain
    Proc Natl Acad Sci U S A 100:8264-9. 2003
  8. ncbi request reprint Down-regulation of hsa-miR-10a in chronic myeloid leukemia CD34+ cells increases USF2-mediated cell growth
    Xabier Agirre
    Foundation for Applied Medical Research, Division of Cancer, Clinica Universitaria, University of Navarre, Pamplona, Spain
    Mol Cancer Res 6:1830-40. 2008
  9. pmc Effect of adenovirus-mediated RNA interference on endogenous microRNAs in a mouse model of multidrug resistance protein 2 gene silencing
    Iñigo Narvaiza
    Division of Gene Therapy and Hepatology, CIMA, University of Navarra, Pio XII 55, 31008 Pamplona, Spain
    J Virol 80:12236-47. 2006
  10. ncbi request reprint Intratumoral injection of dendritic cells transduced by an SV40-based vector expressing interleukin-15 induces curative immunity mediated by CD8+ T lymphocytes and NK cells
    Maria Vera
    Division of Hepatology and Gene Therapy, CIMA, School of Medicine, University of Navarra, Pio XII 55, 31008 Pamplona, Spain
    Mol Ther 12:950-9. 2005

Collaborators

Detail Information

Publications25

  1. pmc Requirements for gene silencing mediated by U1 snRNA binding to a target sequence
    Xabi Abad
    Division of Hepatology and Gene Therapy, CIMA UNAV Pio XII, 55, 31008 Pamplona, Spain
    Nucleic Acids Res 36:2338-52. 2008
    ....
  2. pmc Increased in vivo inhibition of gene expression by combining RNA interference and U1 inhibition
    Lorea Blázquez
    Department of Hepatology and Gene Therapy, Center for Applied Medical Research CIMA, University of Navarra, Pio XII 55 31008 Pamplona, Spain
    Nucleic Acids Res 40:e8. 2012
    ..Therefore, we believe that the combination of RNAi and U1i will be a good option to block damaging endogenous genes, HBV and other infectious agents in vivo...
  3. pmc Adenovirus VA RNA-derived miRNAs target cellular genes involved in cell growth, gene expression and DNA repair
    Oscar Aparicio
    Digna Biotech and Bioinformatics Unit, CIMA, University of Navarra, Pio XII 55, 31008, Pamplona, Spain
    Nucleic Acids Res 38:750-63. 2010
    ..Some of these genes are important for cell growth, transcription, RNA metabolism and DNA repair. We believe that a mivaRNA-mediated fine tune of the expression of some of these genes could be important in adenovirus cell cycle...
  4. pmc Combination of RNA interference and U1 inhibition leads to increased inhibition of gene expression
    X Abad
    Department of Hepatology and Gene Therapy, Center for Applied Medical Research CIMA, University of Navarra, Pamplona 31008, Spain
    Nucleic Acids Res 38:e136. 2010
    ..Also, the combination of these techniques would allow functional inhibition with a decreased dose of inhibitors, avoiding toxicity due to dose-dependent unwanted effects...
  5. ncbi request reprint U1 snRNP control of 3'-end processing and the therapeutic application of U1 inhibition combined with RNA interference
    L Blazquez
    Department of Hepatology and Gene Therapy, Center for Applied Medical Research CIMA, University of Navarra, Pamplona, Spain
    Curr Mol Med 13:1203-16. 2013
    ..We believe that this review will clarify the hallmarks of U1i technology and will encourage many laboratories to use U1i for functional studies and therapeutic applications...
  6. ncbi request reprint Identification and characterization of RED120: a conserved PWI domain protein with links to splicing and 3'-end formation
    Puri Fortes
    Division of Gene Therapy and Hepatology, CIMA, University of Navarra, Pamplona, Spain
    FEBS Lett 581:3087-97. 2007
    ..These results suggest that RED120 may function to couple splicing with mRNA 3'-end formation...
  7. pmc Inhibiting expression of specific genes in mammalian cells with 5' end-mutated U1 small nuclear RNAs targeted to terminal exons of pre-mRNA
    Puri Fortes
    Department of Medicine, University of Navarra, Irunlarrea 1, Pamplona 31008, Spain
    Proc Natl Acad Sci U S A 100:8264-9. 2003
    ..This could reflect a disruption by the 5' end-mutated U1 snRNA of the definition of the terminal exon as described by the exon definition model...
  8. ncbi request reprint Down-regulation of hsa-miR-10a in chronic myeloid leukemia CD34+ cells increases USF2-mediated cell growth
    Xabier Agirre
    Foundation for Applied Medical Research, Division of Cancer, Clinica Universitaria, University of Navarre, Pamplona, Spain
    Mol Cancer Res 6:1830-40. 2008
    ..004). Our results indicate that down-regulation of hsa-miR-10a may increase USF2 and contribute to the increase in cell proliferation of CML implicating a miRNA in the abnormal behavior of CML...
  9. pmc Effect of adenovirus-mediated RNA interference on endogenous microRNAs in a mouse model of multidrug resistance protein 2 gene silencing
    Iñigo Narvaiza
    Division of Gene Therapy and Hepatology, CIMA, University of Navarra, Pio XII 55, 31008 Pamplona, Spain
    J Virol 80:12236-47. 2006
    ..In summary, this work shows that adenoviral vectors can deliver sufficient shRNAs to mediate inhibition of gene expression without saturating the silencing machinery...
  10. ncbi request reprint Intratumoral injection of dendritic cells transduced by an SV40-based vector expressing interleukin-15 induces curative immunity mediated by CD8+ T lymphocytes and NK cells
    Maria Vera
    Division of Hepatology and Gene Therapy, CIMA, School of Medicine, University of Navarra, Pio XII 55, 31008 Pamplona, Spain
    Mol Ther 12:950-9. 2005
    ....
  11. ncbi request reprint Low surface expression of B7-1 (CD80) is an immunoescape mechanism of colon carcinoma
    Iñigo Tirapu
    Gene Therapy Unit, Department of Medicine, Centro de Investigación Médica Aplicada and Clinica Universitaria, University of Navarra School of Medicine, Pamplona, Spain
    Cancer Res 66:2442-50. 2006
    ..Our findings are similar with the inhibitory role described for the dim CD80 expression on immature dendritic cells, providing an explanation for the low levels of CD80 expression described in various human malignancies...
  12. pmc Cardiotrophin-1 defends the liver against ischemia-reperfusion injury and mediates the protective effect of ischemic preconditioning
    María Iñiguez
    Division of Hepatology and Gene Therapy, Center for Applied Medical Research CIMA, Clinica Universitaria and Medical School, University of Navarra, Pamplona 31008, Spain
    J Exp Med 203:2809-15. 2006
    ..In conclusion, CT-1 is an essential endogenous defense of the liver against I/R and is a key mediator of the protective effect induced by IP...
  13. pmc Adenovirus virus-associated RNA is processed to functional interfering RNAs involved in virus production
    Oscar Aparicio
    Division of Gene Therapy and Hepatology, CIMA, University of Navarra, Pio XII 55, 31008 Pamplona, Spain
    J Virol 80:1376-84. 2006
    ..Thus, svaRNA-mediated silencing could represent a novel mechanism used by adenoviruses to control cellular or viral gene expression...
  14. doi request reprint Epigenetic silencing of the tumor suppressor microRNA Hsa-miR-124a regulates CDK6 expression and confers a poor prognosis in acute lymphoblastic leukemia
    Xabier Agirre
    Hematology Department and Area of Cell Therapy, Clinica Universitaria and Division of Gene Therapy and Hepatology, Foundation for Applied Medical Research, University of Navarra, Pamplona, Spain
    Cancer Res 69:4443-53. 2009
    ..005) in the multivariate analysis. These results provide the grounds for new therapeutic strategies in ALL either targeting the epigenetic regulation of microRNAs and/or directly targeting the CDK6-Rb pathway...
  15. doi request reprint MicroRNA-451 is involved in the self-renewal, tumorigenicity, and chemoresistance of colorectal cancer stem cells
    Nerea Bitarte
    Laboratory of Pharmacogenomics, Division of Oncology, Center for Applied Medical Research, CIMA, University of Navarra, Pamplona, Spain
    Stem Cells 29:1661-71. 2011
    ..Our data suggest that miR-451 is a novel candidate to circumvent recurrence and drug resistance in colorectal cancer and could be used as a marker to predict response to irinotecan in patients with colon carcinoma...
  16. doi request reprint Adenovirus and miRNAs
    Elena Carnero
    Department of Hepatology and Gene Therapy, University of Navarra, Pamplona, Spain
    Biochim Biophys Acta 1809:660-7. 2011
    ..This article is part of a "Special Issue entitled:MicroRNAs in viral gene regulation"...
  17. ncbi request reprint Factors influencing the production of recombinant SV40 vectors
    Maria Vera
    Laboratory of Vector Development, Division of Gene Therapy, Foundation for Applied Medical Research, School of Medicine, University of Navarra, Irunlarrea 1, 31008 Pamplona, Spain
    Mol Ther 10:780-91. 2004
    ..Several methods for reproducible titration of rSV40 viruses have been compared. We believe that these techniques can be widely applied to obtain high concentrations of high-quality rSV40 viruses reproducibly...
  18. ncbi request reprint Methionine adenosyltransferase II beta subunit gene expression provides a proliferative advantage in human hepatoma
    Maria L Martinez-Chantar
    División de Hepatología y Terapia Génica, Departamento de Medicina Interna, Universidad de Navarra, Pamplona, Spain
    Gastroenterology 124:940-8. 2003
    ..The beta subunit has been cloned and shown to lower the K(m) of methionine adenosyltransferase II alpha2 (the MAT2A product) for methionine and to render the enzyme more susceptible to S-adenosylmethionine inhibition...
  19. ncbi request reprint Simian virus-40 as a gene therapy vector
    Maria Vera
    School of Medicine, Foundation for Applied Medical Research, Division of Gene Therapy, Laboratory of Vectors Development, University of Navarra, Pamplona, Spain
    DNA Cell Biol 23:271-82. 2004
    ..Furthermore, the strategies utilized for the development, production, and titering of rSV40 vectors are discussed. Last, the therapeutic applications developed to date are highlighted...
  20. doi request reprint microRNA-451 regulates macrophage migration inhibitory factor production and proliferation of gastrointestinal cancer cells
    Eva Bandres
    Division of Oncology and Hepatology, Center for Applied Medical Research, Clinica Universitaria, University of Navarra, Pamplona, Spain
    Clin Cancer Res 15:2281-90. 2009
    ..Recent evidence has shown that some miRNAs can act as oncogenes or tumor suppressors. This study was conducted to evaluate the potential association of miRNA expression with clinical outcome in patients with gastric cancer...
  21. ncbi request reprint In vitro and in vivo comparative study of chimeric liver-specific promoters
    M Gabriela Kramer
    Division of Hepatology and Gene Therapy, Department of Internal Medicine, School of Medicine, University of Navarra, 31008 Pamplona, Spain
    Mol Ther 7:375-85. 2003
    ..We found that among the various chimeric constructs tested in this work, the alpha1-antitrypsin promoter alone or linked to the albumin or hepatitis B enhancers is the most potent in directing stable gene expression in liver cells...
  22. doi request reprint Insulin-like growth factor I gene transfer to cirrhotic liver induces fibrolysis and reduces fibrogenesis leading to cirrhosis reversion in rats
    Luciano Sobrevals
    Department of Hepatology and Gene Therapy, Center for Applied Medical Research, Pamplona, Spain
    Hepatology 51:912-21. 2010
    ..IGF-I gene therapy may be a useful alternative therapy for patients with advanced cirrhosis without timely access to liver transplantation...
  23. doi request reprint In Vitro Correction of a Pseudoexon-Generating Deep Intronic Mutation in LGMD2A by Antisense Oligonucleotides and Modified Small Nuclear RNAs
    Lorea Blázquez
    Center for Applied Medical Research CIMA, University of Navarra, Pamplona, Spain Neuroscience Area, Health Research Institute Biodonostia, San Sebastian, Spain
    Hum Mutat 34:1387-95. 2013
    ..Finally, transfection of antisense sequences and siRNA downregulation of serine/arginine-rich splicing factor 1 (SRSF1) indicate that binding of this factor to splicing enhancer sequences is involved in pseudoexon activation. ..
  24. pmc Long non-coding RNAs in haematological malignancies
    Andoni Garitano-Trojaola
    Laboratory of Myeloproliferative Syndromes, Oncology Area, Foundation for Applied Medical Research, University of Navarra, Pamplona 31008, Spain
    Int J Mol Sci 14:15386-422. 2013
    ..Furthermore, we discuss the potential of lncRNAs in diagnosis, prognosis and therapy in cancer, with special attention to haematological malignancies. ..
  25. pmc The interaction of the cap-binding complex (CBC) with eIF4G is dispensable for translation in yeast
    Julie Baron-Benhamou
    Gene Expression Programme, European Molecular Biology Laboratory, 69117 Heidelberg, Germany
    RNA 9:654-62. 2003
    ....