Genomes and Genes
Affiliation: University of Navarra
- Requirements for gene silencing mediated by U1 snRNA binding to a target sequenceXabi Abad
Division of Hepatology and Gene Therapy, CIMA UNAV Pio XII, 55, 31008 Pamplona, Spain
Nucleic Acids Res 36:2338-52. 2008....
- Combination of RNA interference and U1 inhibition leads to increased inhibition of gene expressionX Abad
Department of Hepatology and Gene Therapy, Center for Applied Medical Research CIMA, University of Navarra, Pamplona 31008, Spain
Nucleic Acids Res 38:e136. 2010..Also, the combination of these techniques would allow functional inhibition with a decreased dose of inhibitors, avoiding toxicity due to dose-dependent unwanted effects...
- U1 snRNP control of 3'-end processing and the therapeutic application of U1 inhibition combined with RNA interferenceL Blazquez
Department of Hepatology and Gene Therapy, Center for Applied Medical Research CIMA, University of Navarra, Pamplona, Spain
Curr Mol Med 13:1203-16. 2013..We believe that this review will clarify the hallmarks of U1i technology and will encourage many laboratories to use U1i for functional studies and therapeutic applications...
- Identification and characterization of RED120: a conserved PWI domain protein with links to splicing and 3'-end formationPuri Fortes
Division of Gene Therapy and Hepatology, CIMA, University of Navarra, Pamplona, Spain
FEBS Lett 581:3087-97. 2007..These results suggest that RED120 may function to couple splicing with mRNA 3'-end formation...
- Inhibiting expression of specific genes in mammalian cells with 5' end-mutated U1 small nuclear RNAs targeted to terminal exons of pre-mRNAPuri Fortes
Department of Medicine, University of Navarra, Irunlarrea 1, Pamplona 31008, Spain
Proc Natl Acad Sci U S A 100:8264-9. 2003..This could reflect a disruption by the 5' end-mutated U1 snRNA of the definition of the terminal exon as described by the exon definition model...
- Down-regulation of hsa-miR-10a in chronic myeloid leukemia CD34+ cells increases USF2-mediated cell growthXabier Agirre
Foundation for Applied Medical Research, Division of Cancer, Clinica Universitaria, University of Navarre, Pamplona, Spain
Mol Cancer Res 6:1830-40. 2008..004). Our results indicate that down-regulation of hsa-miR-10a may increase USF2 and contribute to the increase in cell proliferation of CML implicating a miRNA in the abnormal behavior of CML...
- Effect of adenovirus-mediated RNA interference on endogenous microRNAs in a mouse model of multidrug resistance protein 2 gene silencingIñigo Narvaiza
Division of Gene Therapy and Hepatology, CIMA, University of Navarra, Pio XII 55, 31008 Pamplona, Spain
J Virol 80:12236-47. 2006..In summary, this work shows that adenoviral vectors can deliver sufficient shRNAs to mediate inhibition of gene expression without saturating the silencing machinery...
- Intratumoral injection of dendritic cells transduced by an SV40-based vector expressing interleukin-15 induces curative immunity mediated by CD8+ T lymphocytes and NK cellsMaria Vera
Division of Hepatology and Gene Therapy, CIMA, School of Medicine, University of Navarra, Pio XII 55, 31008 Pamplona, Spain
Mol Ther 12:950-9. 2005....
- Low surface expression of B7-1 (CD80) is an immunoescape mechanism of colon carcinomaIñigo Tirapu
Gene Therapy Unit, Department of Medicine, Centro de Investigación Médica Aplicada and Clinica Universitaria, University of Navarra School of Medicine, Pamplona, Spain
Cancer Res 66:2442-50. 2006..Our findings are similar with the inhibitory role described for the dim CD80 expression on immature dendritic cells, providing an explanation for the low levels of CD80 expression described in various human malignancies...
- Cardiotrophin-1 defends the liver against ischemia-reperfusion injury and mediates the protective effect of ischemic preconditioningMaría Iñiguez
Division of Hepatology and Gene Therapy, Center for Applied Medical Research CIMA, Clinica Universitaria and Medical School, University of Navarra, Pamplona 31008, Spain
J Exp Med 203:2809-15. 2006..In conclusion, CT-1 is an essential endogenous defense of the liver against I/R and is a key mediator of the protective effect induced by IP...
- Adenovirus VA RNA-derived miRNAs target cellular genes involved in cell growth, gene expression and DNA repairOscar Aparicio
Digna Biotech and Bioinformatics Unit, CIMA, University of Navarra, Pio XII 55, 31008, Pamplona, Spain
Nucleic Acids Res 38:750-63. 2010..Some of these genes are important for cell growth, transcription, RNA metabolism and DNA repair. We believe that a mivaRNA-mediated fine tune of the expression of some of these genes could be important in adenovirus cell cycle...
- MicroRNA-451 is involved in the self-renewal, tumorigenicity, and chemoresistance of colorectal cancer stem cellsNerea Bitarte
Laboratory of Pharmacogenomics, Division of Oncology, Center for Applied Medical Research, CIMA, University of Navarra, Pamplona, Spain
Stem Cells 29:1661-71. 2011..Our data suggest that miR-451 is a novel candidate to circumvent recurrence and drug resistance in colorectal cancer and could be used as a marker to predict response to irinotecan in patients with colon carcinoma...
- Adenovirus and miRNAsElena Carnero
Department of Hepatology and Gene Therapy, University of Navarra, Pamplona, Spain
Biochim Biophys Acta 1809:660-7. 2011..This article is part of a "Special Issue entitled:MicroRNAs in viral gene regulation"...
- Factors influencing the production of recombinant SV40 vectorsMaria Vera
Laboratory of Vector Development, Division of Gene Therapy, Foundation for Applied Medical Research, School of Medicine, University of Navarra, Irunlarrea 1, 31008 Pamplona, Spain
Mol Ther 10:780-91. 2004..Several methods for reproducible titration of rSV40 viruses have been compared. We believe that these techniques can be widely applied to obtain high concentrations of high-quality rSV40 viruses reproducibly...
- Epigenetic silencing of the tumor suppressor microRNA Hsa-miR-124a regulates CDK6 expression and confers a poor prognosis in acute lymphoblastic leukemiaXabier Agirre
Hematology Department and Area of Cell Therapy, Clinica Universitaria and Division of Gene Therapy and Hepatology, Foundation for Applied Medical Research, University of Navarra, Pamplona, Spain
Cancer Res 69:4443-53. 2009..005) in the multivariate analysis. These results provide the grounds for new therapeutic strategies in ALL either targeting the epigenetic regulation of microRNAs and/or directly targeting the CDK6-Rb pathway...
- In vitro correction of a pseudoexon-generating deep intronic mutation in LGMD2A by antisense oligonucleotides and modified small nuclear RNAsLorea Blázquez
Center for Applied Medical Research CIMA, University of Navarra, Pamplona, Spain Neuroscience Area, Health Research Institute Biodonostia, San Sebastian, Spain
Hum Mutat 34:1387-95. 2013..Finally, transfection of antisense sequences and siRNA downregulation of serine/arginine-rich splicing factor 1 (SRSF1) indicate that binding of this factor to splicing enhancer sequences is involved in pseudoexon activation. ..
- Methionine adenosyltransferase II beta subunit gene expression provides a proliferative advantage in human hepatomaMaria L Martinez-Chantar
División de Hepatología y Terapia Génica, Departamento de Medicina Interna, Universidad de Navarra, Pamplona, Spain
Gastroenterology 124:940-8. 2003..The beta subunit has been cloned and shown to lower the K(m) of methionine adenosyltransferase II alpha2 (the MAT2A product) for methionine and to render the enzyme more susceptible to S-adenosylmethionine inhibition...
- Adenovirus virus-associated RNA is processed to functional interfering RNAs involved in virus productionOscar Aparicio
Division of Gene Therapy and Hepatology, CIMA, University of Navarra, Pio XII 55, 31008 Pamplona, Spain
J Virol 80:1376-84. 2006..Thus, svaRNA-mediated silencing could represent a novel mechanism used by adenoviruses to control cellular or viral gene expression...
- Increased in vivo inhibition of gene expression by combining RNA interference and U1 inhibitionLorea Blázquez
Department of Hepatology and Gene Therapy, Center for Applied Medical Research CIMA, University of Navarra, Pio XII 55 31008 Pamplona, Spain
Nucleic Acids Res 40:e8. 2012..Therefore, we believe that the combination of RNAi and U1i will be a good option to block damaging endogenous genes, HBV and other infectious agents in vivo...
- microRNA-451 regulates macrophage migration inhibitory factor production and proliferation of gastrointestinal cancer cellsEva Bandres
Division of Oncology and Hepatology, Center for Applied Medical Research, Clinica Universitaria, University of Navarra, Pamplona, Spain
Clin Cancer Res 15:2281-90. 2009..Recent evidence has shown that some miRNAs can act as oncogenes or tumor suppressors. This study was conducted to evaluate the potential association of miRNA expression with clinical outcome in patients with gastric cancer...
- Simian virus-40 as a gene therapy vectorMaria Vera
School of Medicine, Foundation for Applied Medical Research, Division of Gene Therapy, Laboratory of Vectors Development, University of Navarra, Pamplona, Spain
DNA Cell Biol 23:271-82. 2004..Furthermore, the strategies utilized for the development, production, and titering of rSV40 vectors are discussed. Last, the therapeutic applications developed to date are highlighted...
- Insulin-like growth factor I gene transfer to cirrhotic liver induces fibrolysis and reduces fibrogenesis leading to cirrhosis reversion in ratsLuciano Sobrevals
Department of Hepatology and Gene Therapy, Center for Applied Medical Research, Pamplona, Spain
Hepatology 51:912-21. 2010..Also in this model, SVIGF-I improved liver function and reduced liver fibrosis in association with up-regulation of HGF and MMPs and down-regulation of tissue inhibitor of metalloproteinase 1 (TIMP-1)...
- Transient depletion of specific immune cell populations to improve adenovirus-mediated transgene expression in the liverPilar Alzuguren
Division of Hepatology and Gene Therapy, CIMA, Foundation for Applied Medical Research, University of Navarra, Pamplona, Spain
Liver Int 35:1274-89. 2015..Therefore, safe and simple ways to interfere with these processes are needed. Study ways to deplete specific immune cell populations and their impact on liver-directed gene transfer...
- In vitro and in vivo comparative study of chimeric liver-specific promotersM Gabriela Kramer
Division of Hepatology and Gene Therapy, Department of Internal Medicine, School of Medicine, University of Navarra, 31008 Pamplona, Spain
Mol Ther 7:375-85. 2003..We found that among the various chimeric constructs tested in this work, the alpha1-antitrypsin promoter alone or linked to the albumin or hepatitis B enhancers is the most potent in directing stable gene expression in liver cells...
- U1 interference (U1i) for antiviral approachesLorea Blázquez
Department of Gene Therapy and Hepatology, Center for Applied Medical Research, Pio XII 55, Pamplona, Navarra, 31008, Spain
Adv Exp Med Biol 848:51-69. 2015..This shows that, although several aspects of U1i technology remain to be addressed, U1i and U1i combined with RNAi have great potential as antivirals. ..
- Long non-coding RNAs in haematological malignanciesAndoni Garitano-Trojaola
Laboratory of Myeloproliferative Syndromes, Oncology Area, Foundation for Applied Medical Research, University of Navarra, Pamplona 31008, Spain
Int J Mol Sci 14:15386-422. 2013..Furthermore, we discuss the potential of lncRNAs in diagnosis, prognosis and therapy in cancer, with special attention to haematological malignancies. ..
- The interaction of the cap-binding complex (CBC) with eIF4G is dispensable for translation in yeastJulie Baron-Benhamou
Gene Expression Programme, European Molecular Biology Laboratory, 69117 Heidelberg, Germany
RNA 9:654-62. 2003....