Jose V Castell
Affiliation: University of Valencia
- Allergic hepatitis induced by drugsJose V Castell
Unit for Experimental Hepatology, Research Centre, University Hospital La Fe, Spain
Curr Opin Allergy Clin Immunol 6:258-65. 2006....
- Metabolism and bioactivation of toxicants in the lung. The in vitro cellular approachJose V Castell
Research Center, University Hospital La Fe, Avda de Campanar 21, E 46009 Valencia, Spain
Exp Toxicol Pathol 57:189-204. 2005..Genetic manipulation of lung-derived cells allowing them to re-express key biotransformation enzymes appear to be a promising strategy to improve their functionality and metabolic performance...
- Semi-automatic quantitative RT-PCR to measure CYP induction by drugs in human hepatocytesGabriela Perez
Unidad de Hepatologia Experimental, Centro de Investigacion, Hospital Universitario La Fe, Avda de Campanar 21, E 46009, Valencia, Spain
Toxicol In Vitro 17:643-9. 2003..This technique can be of value, to considerably simplify the identification of drug candidates with potential CYP inducing ability in man...
- Hepatocyte cell lines: their use, scope and limitations in drug metabolism studiesJose V Castell
University Hospital La Fe, Research Centre, Avda, Campanar 21, E 46009 Valencia, Spain
Expert Opin Drug Metab Toxicol 2:183-212. 2006..This paper reviews the features of liver-derived cell lines, their suitability for drug metabolism studies as well as the state-of-the-art of the strategies pursued in order to generate metabolically competent hepatic cell lines...
- Drug metabolism by cultured human hepatocytes: how far are we from the in vivo reality?Xavier Ponsoda
Unit of Experimental Hepatology, Research Centre, University Hospital La Fe, Avda Campanar 21, 46009 Valencia, Spain
Altern Lab Anim 32:101-10. 2004..95). Overall, although differing among assays, the in vitro/in vivo metabolism data for each patient were surprisingly similar. Thus, the variability observed in vitro appears to reflect genuine phenotypic variability among the donors...
- An update on metabolism studies using human hepatocytes in primary cultureMaría José Gómez-Lechón
Unidad de Hepatologia Experimental, Centro de Investigacion, Hospital La Fe, Avda de Campanar 21, 46009 Valencia, Spain
Expert Opin Drug Metab Toxicol 4:837-54. 2008..The variability observed in human hepatocytes reflects the existing phenotypic heterogeneity of cytochrome P450 expression in human liver...
- Presence of fibronectin peptides in saliva of patients with Sjögren's syndrome: a potential indicator of salivary gland destructionFrancisco J Silvestre
Department of Stomatology, Faculty of Medicine and Odontology, University of Valencia, Valencia, Spain
Med Oral Patol Oral Cir Bucal 14:e365-70. 2009..The purpose of this preliminary study was to monitor the degree of destruction of salivary glands in Sjögren disease by the detection of fibronectin peptides in patients' saliva...
- Enhanced steatosis by nuclear receptor ligands: a study in cultured human hepatocytes and hepatoma cells with a characterized nuclear receptor expression profileMarta Moya
Unidad de Hepatologia Experimental, Centro de Investigacion, Hospital Universitario La Fe, Valencia, Spain
Chem Biol Interact 184:376-87. 2010..Our results also suggest that human hepatocytes cultured with free fatty acids offer a highly valuable in vitro system to investigate the pathogenesis and therapeutics of the human fatty liver...
- Liver cell culture techniquesJose V Castell
Unit of Experimental Hepatology, University Hospital La Fe, Valencia, Spain
Methods Mol Biol 481:35-46. 2009..Practical details on how to carry out these cultures and to assess their functionality (CYP activity and ureogenesis) are discussed in this chapter...
- Transcriptional regulation and expression of CYP3A4 in hepatocytesCelia P Martinez Jimenez
Unidad de Hepatologia Experimental, Centro de Investigacion, Hospital Universitario La Fe, Avda de Campanar 21, 46009 Valencia, Spain
Curr Drug Metab 8:185-94. 2007..However, other hepatic models should be considered in drug development for screening purposes owing to the limited availability of human hepatocytes...
- Diclofenac induces apoptosis in hepatocytes by alteration of mitochondrial function and generation of ROSM Jose Gomez-Lechon
Centro de Investigacion, Hospital La Fe, Avda de Campanar 21, E 46009 Valencia, Spain
Biochem Pharmacol 66:2155-67. 2003....
- Diclofenac induces apoptosis in hepatocytesMaría José Gómez-Lechón
Centro de Investigacion, Hospital La Fe, Avda de Campanar 21, E 46009, Valencia, Spain
Toxicol In Vitro 17:675-80. 2003..Finally, the differential cytotoxic and apoptotic effect produced in hepatocytes and non-metabolizing hepatoma cells suggest that CYP-mediated metabolism of diclofenac apoptosis may be related to the apoptotic effect of the drug...