Jose V Castell

Summary

Affiliation: University of Valencia
Country: Spain

Publications

  1. ncbi request reprint Allergic hepatitis induced by drugs
    Jose V Castell
    Unit for Experimental Hepatology, Research Centre, University Hospital La Fe, Spain
    Curr Opin Allergy Clin Immunol 6:258-65. 2006
  2. ncbi request reprint Metabolism and bioactivation of toxicants in the lung. The in vitro cellular approach
    Jose V Castell
    Research Center, University Hospital La Fe, Avda de Campanar 21, E 46009 Valencia, Spain
    Exp Toxicol Pathol 57:189-204. 2005
  3. ncbi request reprint Semi-automatic quantitative RT-PCR to measure CYP induction by drugs in human hepatocytes
    Gabriela Perez
    Unidad de Hepatologia Experimental, Centro de Investigacion, Hospital Universitario La Fe, Avda de Campanar 21, E 46009, Valencia, Spain
    Toxicol In Vitro 17:643-9. 2003
  4. ncbi request reprint Hepatocyte cell lines: their use, scope and limitations in drug metabolism studies
    Jose V Castell
    University Hospital La Fe, Research Centre, Avda, Campanar 21, E 46009 Valencia, Spain
    Expert Opin Drug Metab Toxicol 2:183-212. 2006
  5. ncbi request reprint Down-regulation of human CYP3A4 by the inflammatory signal interleukin-6: molecular mechanism and transcription factors involved
    Ramiro Jover
    Unidad de Hepatologia Experimental, Centro de Investigacion, Hospital Universitario La Fe, E 46009 Valencia, Spain
    FASEB J 16:1799-801. 2002
  6. ncbi request reprint Drug metabolism by cultured human hepatocytes: how far are we from the in vivo reality?
    Xavier Ponsoda
    Unit of Experimental Hepatology, Research Centre, University Hospital La Fe, Avda Campanar 21, 46009 Valencia, Spain
    Altern Lab Anim 32:101-10. 2004
  7. ncbi request reprint Strategies and molecular probes to investigate the role of cytochrome P450 in drug metabolism: focus on in vitro studies
    M Teresa Donato
    Unit of Experimental Hepatology, Research Centre, University Hospital La Fe and Department of Biochemistry and Molecular Biology, University of Valencia, Valencia, Spain
    Clin Pharmacokinet 42:153-78. 2003
  8. ncbi request reprint An update on metabolism studies using human hepatocytes in primary culture
    María José Gómez-Lechón
    Unidad de Hepatologia Experimental, Centro de Investigacion, Hospital La Fe, Avda de Campanar 21, 46009 Valencia, Spain
    Expert Opin Drug Metab Toxicol 4:837-54. 2008
  9. ncbi request reprint Presence of fibronectin peptides in saliva of patients with Sjögren's syndrome: a potential indicator of salivary gland destruction
    Francisco J Silvestre
    Department of Stomatology, Faculty of Medicine and Odontology, University of Valencia, Valencia, Spain
    Med Oral Patol Oral Cir Bucal 14:e365-70. 2009
  10. doi request reprint Enhanced steatosis by nuclear receptor ligands: a study in cultured human hepatocytes and hepatoma cells with a characterized nuclear receptor expression profile
    Marta Moya
    Unidad de Hepatologia Experimental, Centro de Investigacion, Hospital Universitario La Fe, Valencia, Spain
    Chem Biol Interact 184:376-87. 2010

Collaborators

  • Ramiro Jover
  • Marta Moya
  • María José Gómez-Lechón
  • M Teresa Donato
  • M Jose Gomez-Lechon
  • Xavier Ponsoda
  • Laia Tolosa
  • Francisco J Silvestre
  • Gabriela Perez-Cataldo
  • Celia P Martinez-Jimenez
  • Gabriela Perez
  • Enrique O'Connor
  • Teresa Donato
  • Angel Puente
  • Jose V Bagan
  • Maria Teresa Donato
  • Beatriz Tabares

Detail Information

Publications15

  1. ncbi request reprint Allergic hepatitis induced by drugs
    Jose V Castell
    Unit for Experimental Hepatology, Research Centre, University Hospital La Fe, Spain
    Curr Opin Allergy Clin Immunol 6:258-65. 2006
    ....
  2. ncbi request reprint Metabolism and bioactivation of toxicants in the lung. The in vitro cellular approach
    Jose V Castell
    Research Center, University Hospital La Fe, Avda de Campanar 21, E 46009 Valencia, Spain
    Exp Toxicol Pathol 57:189-204. 2005
    ..Genetic manipulation of lung-derived cells allowing them to re-express key biotransformation enzymes appear to be a promising strategy to improve their functionality and metabolic performance...
  3. ncbi request reprint Semi-automatic quantitative RT-PCR to measure CYP induction by drugs in human hepatocytes
    Gabriela Perez
    Unidad de Hepatologia Experimental, Centro de Investigacion, Hospital Universitario La Fe, Avda de Campanar 21, E 46009, Valencia, Spain
    Toxicol In Vitro 17:643-9. 2003
    ..This technique can be of value, to considerably simplify the identification of drug candidates with potential CYP inducing ability in man...
  4. ncbi request reprint Hepatocyte cell lines: their use, scope and limitations in drug metabolism studies
    Jose V Castell
    University Hospital La Fe, Research Centre, Avda, Campanar 21, E 46009 Valencia, Spain
    Expert Opin Drug Metab Toxicol 2:183-212. 2006
    ..This paper reviews the features of liver-derived cell lines, their suitability for drug metabolism studies as well as the state-of-the-art of the strategies pursued in order to generate metabolically competent hepatic cell lines...
  5. ncbi request reprint Down-regulation of human CYP3A4 by the inflammatory signal interleukin-6: molecular mechanism and transcription factors involved
    Ramiro Jover
    Unidad de Hepatologia Experimental, Centro de Investigacion, Hospital Universitario La Fe, E 46009 Valencia, Spain
    FASEB J 16:1799-801. 2002
    ..From a clinical point of view, these findings could be relevant in the development of therapeutic cytokines with a less repressive effect on hepatic drug-metabolizing enzymes...
  6. ncbi request reprint Drug metabolism by cultured human hepatocytes: how far are we from the in vivo reality?
    Xavier Ponsoda
    Unit of Experimental Hepatology, Research Centre, University Hospital La Fe, Avda Campanar 21, 46009 Valencia, Spain
    Altern Lab Anim 32:101-10. 2004
    ..95). Overall, although differing among assays, the in vitro/in vivo metabolism data for each patient were surprisingly similar. Thus, the variability observed in vitro appears to reflect genuine phenotypic variability among the donors...
  7. ncbi request reprint Strategies and molecular probes to investigate the role of cytochrome P450 in drug metabolism: focus on in vitro studies
    M Teresa Donato
    Unit of Experimental Hepatology, Research Centre, University Hospital La Fe and Department of Biochemistry and Molecular Biology, University of Valencia, Valencia, Spain
    Clin Pharmacokinet 42:153-78. 2003
    ..Primary cultured hepatocytes are still the unique in vitro model that allows global examination of the inductive potential of a drug...
  8. ncbi request reprint An update on metabolism studies using human hepatocytes in primary culture
    María José Gómez-Lechón
    Unidad de Hepatologia Experimental, Centro de Investigacion, Hospital La Fe, Avda de Campanar 21, 46009 Valencia, Spain
    Expert Opin Drug Metab Toxicol 4:837-54. 2008
    ..The variability observed in human hepatocytes reflects the existing phenotypic heterogeneity of cytochrome P450 expression in human liver...
  9. ncbi request reprint Presence of fibronectin peptides in saliva of patients with Sjögren's syndrome: a potential indicator of salivary gland destruction
    Francisco J Silvestre
    Department of Stomatology, Faculty of Medicine and Odontology, University of Valencia, Valencia, Spain
    Med Oral Patol Oral Cir Bucal 14:e365-70. 2009
    ..The purpose of this preliminary study was to monitor the degree of destruction of salivary glands in Sjögren disease by the detection of fibronectin peptides in patients' saliva...
  10. doi request reprint Enhanced steatosis by nuclear receptor ligands: a study in cultured human hepatocytes and hepatoma cells with a characterized nuclear receptor expression profile
    Marta Moya
    Unidad de Hepatologia Experimental, Centro de Investigacion, Hospital Universitario La Fe, Valencia, Spain
    Chem Biol Interact 184:376-87. 2010
    ..Our results also suggest that human hepatocytes cultured with free fatty acids offer a highly valuable in vitro system to investigate the pathogenesis and therapeutics of the human fatty liver...
  11. doi request reprint HepG2 cells simultaneously expressing five P450 enzymes for the screening of hepatotoxicity: identification of bioactivable drugs and the potential mechanism of toxicity involved
    Laia Tolosa
    Unidad de Hepatologia Experimental, Centro de Investigacion, Instituto de Investigación Sanitaria Hospital La Fe, Valencia, Avda de Campanar 21, 46009 Valencia, Spain
    Arch Toxicol 87:1115-27. 2013
    ..Overall, our results indicate that this assay may be a suitable new in vitro approach for early screening of compounds to identify bioactivable hepatotoxins and the mechanism(s) involved in their toxicity...
  12. doi request reprint Liver cell culture techniques
    Jose V Castell
    Unit of Experimental Hepatology, University Hospital La Fe, Valencia, Spain
    Methods Mol Biol 481:35-46. 2009
    ..Practical details on how to carry out these cultures and to assess their functionality (CYP activity and ureogenesis) are discussed in this chapter...
  13. ncbi request reprint Transcriptional regulation and expression of CYP3A4 in hepatocytes
    Celia P Martinez-Jimenez
    Unidad de Hepatologia Experimental, Centro de Investigacion, Hospital Universitario La Fe, Avda de Campanar 21, 46009 Valencia, Spain
    Curr Drug Metab 8:185-94. 2007
    ..However, other hepatic models should be considered in drug development for screening purposes owing to the limited availability of human hepatocytes...
  14. ncbi request reprint Diclofenac induces apoptosis in hepatocytes by alteration of mitochondrial function and generation of ROS
    M Jose Gomez-Lechon
    Centro de Investigacion, Hospital La Fe, Avda de Campanar 21, E 46009 Valencia, Spain
    Biochem Pharmacol 66:2155-67. 2003
    ....
  15. ncbi request reprint Diclofenac induces apoptosis in hepatocytes
    María José Gómez-Lechón
    Centro de Investigacion, Hospital La Fe, Avda de Campanar 21, E 46009, Valencia, Spain
    Toxicol In Vitro 17:675-80. 2003
    ..Finally, the differential cytotoxic and apoptotic effect produced in hepatocytes and non-metabolizing hepatoma cells suggest that CYP-mediated metabolism of diclofenac apoptosis may be related to the apoptotic effect of the drug...