Research Topics
Genomes and Genes | Tsutomu FujimuraSummaryAffiliation: Universidad de Salamanca Country: Spain Publications
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Publications
Cap snatching of yeast L-A double-stranded RNA virus can operate in trans and requires viral polymerase actively engaging in transcriptionTsutomu Fujimura
Instituto de Biología Funcional y Genómica, CSIC Universidad de Salamanca, Salamanca 37007, Spain
J Biol Chem 287:12797-804. 2012..This will allow L-A virus to efficiently produce capsid proteins to form new virions when Pol is actively engaging in transcription. The coordination may also minimize the risk of accidental capping of nonviral RNA when Pol is dormant...- Cap-snatching mechanism in yeast L-A double-stranded RNA virusTsutomu Fujimura
Instituto de Biología Funcional y Genómica, Consejo Superior de Investigaciones Cientificas Universidad de Salamanca, Edificio Departamental, Avenida del Campo Charro, Salamanca 37007, Spain
Proc Natl Acad Sci U S A 108:17667-71. 2011.... 
Bipartite 3'-cis-acting signal for replication in yeast 23 S RNA virus and its repairTsutomu Fujimura
Instituto de Microbiologia Bioquimica, Consejo Superior de Investigaciones Cientificas Universidad de Salamanca, Avda del Campo Charro s n, Salamanca 37007, Spain
J Biol Chem 279:13215-23. 2004..We discuss possible mechanisms for this 3' end repair in vivo...- Yeast double-stranded RNA virus L-A deliberately synthesizes RNA transcripts with 5'-diphosphateTsutomu Fujimura
Instituto de Microbiología Bioquímica Departamento de Microbiología y Genética, Consejo Superior de Investigaciones Cientificas Universidad de Salamanca, Salamanca 37007, Spain
J Biol Chem 285:22911-8. 2010..We also discuss the implication of these findings on translation of viral RNA. Because the viral transcript has no conventional 5'-cap structure, this work may shed light on the metabolism of non-self-RNA in yeast... - Native replication intermediates of the yeast 20 S RNA virus have a single-stranded RNA backboneTsutomu Fujimura
Instituto de Microbiologia Bioquimica, Consejo Superior de Investigaciones Cientificas Universidad de Salamanca, Avda del Campo Charro s n Salamanca 37007, Spain
J Biol Chem 280:7398-406. 2005..Therefore, W, a dsRNA form of 20 S RNA that accumulates in yeast cells grown at 37 degrees C, is not an intermediate in the 20 S RNA replication cycle, but a by-product... - Interactions of the RNA polymerase with the viral genome at the 5'- and 3'-ends contribute to 20S RNA narnavirus persistence in yeastTsutomu Fujimura
Instituto de Microbiología Bioquímica Departamento de Microbiología y Genética, Consejo Superior de Investigaciones Cientificas Universidad de Salamanca, 37007 Salamanca, Spain
J Biol Chem 282:19011-9. 2007..We will discuss implications of these findings especially on how the virus escapes from mRNA degradation pathways and resides in the cytoplasm persistently despite the lack of a protective capsid... - Launching the yeast 23S RNA Narnavirus shows 5' and 3' cis-acting signals for replicationRosa Esteban
Instituto de Microbiologia Bioquimica, Consejo Superior de Investigaciones CientificasUniversidad de Salamanca, 37007 Salamanca, Spain
Proc Natl Acad Sci U S A 100:2568-73. 2003.... - 20S RNA narnavirus defies the antiviral activity of SKI1/XRN1 in Saccharomyces cerevisiaeRosa Esteban
Instituto de Microbiologia Bioquimica, Departamento de Microbiologia y Genetica, Consejo Superior de Investigaciones Cientificas Universidad de Salamanca, Salamanca 37007, Spain
J Biol Chem 283:25812-20. 2008..Given that 20S RNA virus resides and replicates in the cytoplasm without a protective capsid, our results suggest that the strong secondary structure at the 5'-end is crucial for the 20S RNA virus to evade the host SKI1/XRN1 defense... - Launching of the yeast 20 s RNA narnavirus by expressing the genomic or antigenomic viral RNA in vivoRosa Esteban
, , Salamanca 37007, Spain. mrosagugu.usal.es
J Biol Chem 280:33725-34. 2005..Again, a cluster of four Cs at the 3'-end in the antigenomic strand was essential for replication. In this work, we also present the first conclusive evidence that 20 S and 23 S RNA viruses are independent replicons... - The bipartite 3'-cis-acting signal for replication is required for formation of a ribonucleoprotein complex in vivo between the viral genome and its RNA polymerase in yeast 23 S RNA virusTsutomu Fujimura
, , 37007 Spain
J Biol Chem 279:44219-28. 2004.... - Ancient ubiquitous protein 1 binds to the conserved membrane-proximal sequence of the cytoplasmic tail of the integrin alpha subunits that plays a crucial role in the inside-out signaling of alpha IIbbeta 3Atsushi Kato
Division of Hematology, Department of Internal Medicine and Division of Biochemical Analysis, Central Laboratory of Medical Sciences, Juntendo University School of Medicine, 2 1 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
J Biol Chem 277:28934-41. 2002..As Aup1 possesses domains related to signal transduction, these results suggest involvement of Aup1 in the integrin signaling... - Sphingosine-dependent protein kinase-1, directed to 14-3-3, is identified as the kinase domain of protein kinase C deltaAkikazu Hamaguchi
Department of Pathobiology, University of Washington, Seattle, Washington 98122-4302, USA
J Biol Chem 278:41557-65. 2003..The high susceptibility of SDK1 to Sph and DMS accounts for their important modulatory role in signal transduction...