R Borges

Summary

Affiliation: Universidad de La Laguna
Country: Spain

Publications

  1. ncbi request reprint Morphological and functional characterization of beige mouse adrenomedullary secretory vesicles
    R Borges
    Unidad de Farmacologia, Facultad de Medicina, Universidad de La Laguna, Spain
    Cell Tissue Res 304:159-64. 2001
  2. ncbi request reprint Measuring secretion in chromaffin cells using electrophysiological and electrochemical methods
    R Borges
    Unidad de Farmacologia, Facultad de Medicina, Universidad de La Laguna, Tenerife, Spain
    Acta Physiol (Oxf) 192:173-84. 2008
  3. doi request reprint Vesicular Ca(2+) mediates granule motion and exocytosis
    Ricardo Borges
    Unidad de Farmacologia, Facultad de Medicina, Universidad de La Laguna, Tenerife, Spain
    Cell Calcium 51:338-41. 2012
  4. doi request reprint Chromogranins as regulators of exocytosis
    Ricardo Borges
    Unidad de Farmacologia, Facultad de Medicina, Universidad de La Laguna, Tenerife, Spain
    J Neurochem 114:335-43. 2010
  5. doi request reprint Intravesicular factors controlling exocytosis in chromaffin cells
    Ricardo Borges
    Unit of Pharmacology, Medical School, La Laguna University, 38071 La Laguna, Tenerife, Spain
    Cell Mol Neurobiol 30:1359-64. 2010
  6. ncbi request reprint Pharmacological regulation of the late steps of exocytosis
    Ricardo Borges
    Unidad de Farmacologia, Facultad de Medicina, Universidad de La Laguna, Tenerife, Spain
    Ann N Y Acad Sci 971:184-92. 2002
  7. ncbi request reprint A simple way to build a grinder for carbon-fibre electrodes for amperometry or voltammetry
    Ricardo Borges
    Unidad de Farmacología Facultad de Medicina, Universidad de La Laguna, Tenerife, Spain
    Pflugers Arch 450:280-2. 2005
  8. ncbi request reprint Chromaffin cell research in the new millennium
    Ricardo Borges
    Unidad de Farmacologia, Facultad de Medicina, Universidad de La Laguna, E 38071, Tenerife, La Laguna, Spain
    Trends Pharmacol Sci 23:53-5. 2002
  9. doi request reprint Chromogranins A and B are key proteins in amine accumulation, but the catecholamine secretory pathway is conserved without them
    Jésica Díaz-Vera
    Unidad de Farmacologia, Universidad de La Laguna, La Laguna, Tenerife, Spain
    FASEB J 26:430-8. 2012
  10. ncbi request reprint cAmp modulates exocytotic kinetics and increases quantal size in chromaffin cells
    J D Machado
    , Facultad de Medicina, Universidad de La Laguna, Tenerife, Spain
    Mol Pharmacol 60:514-20. 2001

Collaborators

  • F Valladares
  • R Gutierrez
  • Rafael Lujan
  • Sushil Mahata
  • Diego Alvarez de la Rosa
  • Alexei Verkhratsky
  • Ana M Cárdenas
  • A Nistri
  • E Carbone
  • Marcial Camacho
  • Monica S Montesinos
  • José D Machado
  • Jésica Díaz-Vera
  • J David Machado
  • Juan R Hernández-Fernaud
  • Natalia Domínguez
  • Yezer G Morales
  • O Humberto Viveros
  • David J Machado
  • L Diaz-Flores
  • J D Machado
  • Antonio G Garcia
  • Alvaro O Ardiles
  • Patricia Neco
  • M Camacho
  • Miguel A Brioso
  • J Francisco Gomez
  • Araceli Morales
  • Carmen Alonso
  • José F Gómez
  • José David Machado
  • Beatriz Beltrán
  • Federico Calegari
  • Marta R Pardo
  • Wieland B Huttner
  • Carmen M Alvarez
  • DANIEL T O'CONNOR
  • H Varela
  • Emilia Carmona
  • Jesica Diaz
  • H Alvarez-Arguelles
  • Agustin Castañeyra
  • Javier Alvarez
  • Javier García-Sancho
  • Eva Ales
  • Rodrigo Toro
  • Italo G Mora
  • Lorena Villarroel
  • Antonio M García-De-Diego
  • Manuel Criado
  • Verónica L Lagos
  • Luis Gandia
  • Jaime Maripillan
  • Luis M Gutierrez
  • Alvaro Villarroel
  • Daniel Giner
  • Salvador Viniegra
  • M S Montesinos
  • Jose L Sanchez
  • L Gandia
  • Gema Betancor
  • J F Gomez
  • A Morales
  • A G Garcia
  • M G Lopez
  • J A Gilabert
  • M Villarroya
  • J J Ballesta
  • M J Hidalgo

Detail Information

Publications29

  1. ncbi request reprint Morphological and functional characterization of beige mouse adrenomedullary secretory vesicles
    R Borges
    Unidad de Farmacologia, Facultad de Medicina, Universidad de La Laguna, Spain
    Cell Tissue Res 304:159-64. 2001
    ..We conclude that CG, but not the abnormal-sized vesicles found in BM chromaffin cells contribute to the catecholamine secretion and that abnormal secretory granules are not present in adrenergic cell lineage...
  2. ncbi request reprint Measuring secretion in chromaffin cells using electrophysiological and electrochemical methods
    R Borges
    Unidad de Farmacologia, Facultad de Medicina, Universidad de La Laguna, Tenerife, Spain
    Acta Physiol (Oxf) 192:173-84. 2008
    ..In this brief review, we will outline the strengths and limitations of amperometric and electrophysiological methods and highlight the major contribution obtained with the use of these techniques in chromaffin cells...
  3. doi request reprint Vesicular Ca(2+) mediates granule motion and exocytosis
    Ricardo Borges
    Unidad de Farmacologia, Facultad de Medicina, Universidad de La Laguna, Tenerife, Spain
    Cell Calcium 51:338-41. 2012
    ..We will also address the potential role of vesicular Ca(2+) in two major processes in secretory cells, vesicle movement and exocytosis...
  4. doi request reprint Chromogranins as regulators of exocytosis
    Ricardo Borges
    Unidad de Farmacologia, Facultad de Medicina, Universidad de La Laguna, Tenerife, Spain
    J Neurochem 114:335-43. 2010
    ..In this review, we will discuss the roles of Cgs A and B in maintaining the intravesicular environment of secretory vesicles and in exocytosis, bringing together the most recent findings from adrenal chromaffin cells...
  5. doi request reprint Intravesicular factors controlling exocytosis in chromaffin cells
    Ricardo Borges
    Unit of Pharmacology, Medical School, La Laguna University, 38071 La Laguna, Tenerife, Spain
    Cell Mol Neurobiol 30:1359-64. 2010
    ..We also offer a model to explain the regulation of exocytosis by the intravesicular media that conciliate the experimentally obtained data...
  6. ncbi request reprint Pharmacological regulation of the late steps of exocytosis
    Ricardo Borges
    Unidad de Farmacologia, Facultad de Medicina, Universidad de La Laguna, Tenerife, Spain
    Ann N Y Acad Sci 971:184-92. 2002
    ....
  7. ncbi request reprint A simple way to build a grinder for carbon-fibre electrodes for amperometry or voltammetry
    Ricardo Borges
    Unidad de Farmacología Facultad de Medicina, Universidad de La Laguna, Tenerife, Spain
    Pflugers Arch 450:280-2. 2005
    ..This apparatus is expensive because it needs a very stable motor, a diamond surface and a micromanipulator. We describe the construction of a cheap precision grinder using a computer drive and an old microscope...
  8. ncbi request reprint Chromaffin cell research in the new millennium
    Ricardo Borges
    Unidad de Farmacologia, Facultad de Medicina, Universidad de La Laguna, E 38071, Tenerife, La Laguna, Spain
    Trends Pharmacol Sci 23:53-5. 2002
  9. doi request reprint Chromogranins A and B are key proteins in amine accumulation, but the catecholamine secretory pathway is conserved without them
    Jésica Díaz-Vera
    Unidad de Farmacologia, Universidad de La Laguna, La Laguna, Tenerife, Spain
    FASEB J 26:430-8. 2012
    ....
  10. ncbi request reprint cAmp modulates exocytotic kinetics and increases quantal size in chromaffin cells
    J D Machado
    , Facultad de Medicina, Universidad de La Laguna, Tenerife, Spain
    Mol Pharmacol 60:514-20. 2001
    ..These data indicate that cyclic AMP/PKA might favor the granule aggregation before its fusion with cell membrane and slow the late step of the exocytotic process...
  11. ncbi request reprint Nongenomic regulation of the kinetics of exocytosis by estrogens
    José D Machado
    Unidad de Farmacologia, Facultad de Medicina, Universidad de La Laguna, Tenerife, Spain
    J Pharmacol Exp Ther 301:631-7. 2002
    ..Our data suggest the presence of membrane sites that regulate both the exocytotic phenomenon and the total catecholamine release with high and low affinity, respectively...
  12. ncbi request reprint Hydralazine reduces the quantal size of secretory events by displacement of catecholamines from adrenomedullary chromaffin secretory vesicles
    José D Machado
    Unidad de Farmacologia, Facultad de Medicina, Universidad de La Laguna, Tenerife, Spain
    Circ Res 91:830-6. 2002
    ..We proposed that these novel effects could explain many of the therapeutic and side effects of this drug that are likely exerted in sympathetic nerve terminals...
  13. pmc Secretory and radioligand binding studies on muscarinic receptors in bovine and feline chromaffin cells
    J J Ballesta
    Departmento de Neuroquimica, Universidad de Alicante, Facultad de Medicina, Spain
    J Physiol 418:411-26. 1989
    ..However, it is unclear why the bovine behaves differently from the feline chromaffin cell as far as the muscarine-evoked effects are concerned.(ABSTRACT TRUNCATED AT 400 WORDS)..
  14. ncbi request reprint [Exocytosis as the mechanism for neural communication. A view from chromaffin cells]
    M Camacho
    Unidad de Farmacologia, Facultad de Medicina, Universidad de La Laguna Tenerife, Espana
    Rev Neurol 36:355-60. 2003
    ..We also review the novel mechanisms implicated in the regulation of the late steps of exocytosis as well as their possible role as target for drug therapy..
  15. ncbi request reprint Intragranular pH rapidly modulates exocytosis in adrenal chromaffin cells
    Marcial Camacho
    Unidad de Farmacologia, Facultad de Medicina, Universidad de La Laguna, Tenerife, Spain
    J Neurochem 96:324-34. 2006
    ..We therefore propose that the regulation of vesicular pH is, at least partially, a necessary step in the modulation of the kinetics of exocytosis and quantal size operated by some cell signals...
  16. doi request reprint Chromogranin B gene ablation reduces the catecholamine cargo and decelerates exocytosis in chromaffin secretory vesicles
    Jésica Díaz-Vera
    Unit of Pharmacology, Medical School, La Laguna University, 38071 La Laguna, Tenerife, Spain
    J Neurosci 30:950-7. 2010
    ..We conclude that CgB, like CgA, is a highly efficient system directly involved in monoamine accumulation and in the kinetics of exocytosis from LDCVs...
  17. ncbi request reprint A novel nongenomic action of estrogens: the regulation of exocytotic kinetics
    J David Machado
    Unidad de Farmacologia, Facultad de Medicin, Universidad de La Laguna, Tenerife, Spain
    Ann N Y Acad Sci 971:284-6. 2002
  18. doi request reprint Chromogranins A and B as regulators of vesicle cargo and exocytosis
    José D Machado
    Unit of Pharmacology, Medical School, La Laguna University, 38071 La Laguna, Tenerife, Spain
    Cell Mol Neurobiol 30:1181-7. 2010
    ..We will discuss the roles of Cgs A and B in maintaining the intravesicular environment of secretory vesicles and in exocytosis, bringing together the most recent findings from adrenal chromaffin cells...
  19. doi request reprint The crucial role of chromogranins in storage and exocytosis revealed using chromaffin cells from chromogranin A null mouse
    Monica S Montesinos
    Unit of Pharmacology, Medical School, University of La Laguna, 38071 Tenerife, Spain
    J Neurosci 28:3350-8. 2008
    ..We conclude that intravesicular proteins are highly efficient systems directly implicated in transmitter accumulation and in the control of neurosecretion...
  20. doi request reprint Good practices in single-cell amperometry
    David J Machado
    Pharmacology Unit, Faculty of Medicine, University of La Laguna, Tenerife, Spain
    Methods Mol Biol 440:297-313. 2008
    ..We concentrate on cultured chromaffin cells, although most of the information is equally applicable to other cell types...
  21. ncbi request reprint Histogenesis and morphofunctional characteristics of chromaffin cells
    L Diaz-Flores
    Department of Pathology and Histology, School of Medicine, La Laguna University, Canary Islands, Spain
    Acta Physiol (Oxf) 192:145-63. 2008
    ..Moreover, the relationship of chromaffin cells with other tissue components of the adrenal medulla is also revised, comprising the ganglion cells, sustentacular cells, nerves and connective-vascular tissue...
  22. pmc The quantal secretion of catecholamines is impaired by the accumulation of beta-adrenoceptor antagonists into chromaffin cell vesicles
    Monica S Montesinos
    Unidad de Farmacologia, Facultad de Medicina, Universidad de La Laguna, Tenerife, Spain
    Br J Pharmacol 159:1548-56. 2010
    ..This work aimed at providing a new explanation based on the interference of these drugs with the functional accumulation of catecholamines within neurosecretory vesicles...
  23. ncbi request reprint New approaches for analysis of amperometrical recordings
    J Francisco Gomez
    Unidad de Farmacologia, Facultad de Medicina, and Departamento de Física Fundamental y Experimental, Universidad de La Laguna, Tenerife, Spain
    Ann N Y Acad Sci 971:647-54. 2002
    ..In addition, we have designed a new filtering method for amperometric data. It provides an excellent tool to enhance the signal/noise ratio with minimal artifacts. This filter allows one to obtain more reliable spike parameters...
  24. doi request reprint Intravesicular calcium release mediates the motion and exocytosis of secretory organelles: a study with adrenal chromaffin cells
    Marcial Camacho
    Unidad de Farmacologia, Facultad de Medicina, Universidad de La Laguna Tenerife, E 38071 La Laguna, Spain
    J Biol Chem 283:22383-9. 2008
    ..We conclude that the gradient of pH of secretory vesicles might be involved in the homeostatic regulation of cytosolic Ca2+ and in two of the major functions of secretory cells, vesicle motion and exocytosis...
  25. ncbi request reprint Blocking effects of otilonium on Ca2+ channels and secretion in rat chromaffin cells
    L Gandia
    Departamento de Farmacología y Medicina Física, Universidad de La Laguna, Tenerife, Spain
    Eur J Pharmacol 298:199-205. 1996
    ..This would lead to a limitation in the rise in cytosolic Ca2+ at secretory sites and to inhibition of catecholamine release in response to stimulation of chromaffin cells...
  26. ncbi request reprint New roles of myosin II during vesicle transport and fusion in chromaffin cells
    Patricia Neco
    Instituto de Neurociencias, Centro Mixto CSIC Universidad Miguel Hernández, E 03550 Alicante, Spain
    J Biol Chem 279:27450-7. 2004
    ....
  27. ncbi request reprint A rapid exocytosis mode in chromaffin cells with a neuronal phenotype
    Alvaro O Ardiles
    Centro de Neurociencia de Valparaiso, Universidad de Valparaiso, Chile
    J Neurochem 99:29-41. 2006
    ..Such rapid exocytosis mode could be caused at least in part by a better coupling between secretory vesicles and VGCC...
  28. doi request reprint Chromaffin cells at the beginning of the 21st century
    Ricardo Borges
    Acta Physiol (Oxf) 192:143-4. 2008
  29. ncbi request reprint Calcium signaling and exocytosis in adrenal chromaffin cells
    Antonio G Garcia
    Instituto Teofilo Hernando, Departamento de Farmacologia y Terapeutica, and Servicio de Farmacología Clínica e Instituto Universitario de Investigación Gerontológica y Metabólica, Hospital Universitario de la Princesa, Madrid, Spain
    Physiol Rev 86:1093-131. 2006
    ..We propose that chromaffin cells have developed functional triads that are formed by calcium channels, the ER, and the mitochondria and locally control the [Ca2+]c that regulate the early and late steps of exocytosis...