Maria J Bonete

Summary

Affiliation: Universidad de Alicante
Country: Spain

Publications

  1. ncbi request reprint Glucose dehydrogenase from the halophilic Archaeon Haloferax mediterranei: enzyme purification, characterisation and N-terminal sequence
    M J Bonete
    División de Bioquímica, Facultad de Ciencias, Universidad de Alicante, Spain
    FEBS Lett 383:227-9. 1996
  2. ncbi request reprint Occurrence of two different glutamate dehydrogenase activities in the halophilic bacterium Salinibacter ruber
    Maria Jose Bonete
    Division of Biochemistry and Molecular Biology, University of Alicante, 03080, Alicante, Spain
    FEMS Microbiol Lett 226:181-6. 2003
  3. pmc Biodiversity of Archaea and floral of two inland saltern ecosystems in the Alto Vinalopó Valley, Spain
    Basilio Zafrilla
    División de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Alicante, AP, 99, E 03080 Alicante, Spain
    Saline Systems 6:10. 2010
  4. ncbi request reprint 2-Hydroxyacid dehydrogenase from Haloferax mediterranei, a D-isomer-specific member of the 2-hydroxyacid dehydrogenase family
    M J Bonete
    Departamento de Bioquimica, Facultad de Ciencias, Universidad de Alicante, Ap 99, Alicante, Spain
    Biochimie 82:1143-50. 2000
  5. ncbi request reprint Alpha-amylase activity from the halophilic archaeon Haloferax mediterranei
    F Perez-Pomares
    División de Bioquímica, Facultad de Ciencias, Universidad de Alicante, Apartado 99, 03080 Alicante, Spain
    Extremophiles 7:299-306. 2003
  6. ncbi request reprint Analysis of acidic surface of Haloferax mediterranei glucose dehydrogenase by site-directed mutagenesis
    J Esclapez
    Departamento de Agroquimica y Bioquimica, División de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Alicante, Ap 99, E 03080 Alicante, Spain
    FEBS Lett 581:837-42. 2007
  7. ncbi request reprint Assimilatory nitrate reductase from the haloarchaeon Haloferax mediterranei: purification and characterisation
    R M Martínez-Espinosa
    División de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Alicante, Ap 99, E 03080, Alicante, Spain
    FEMS Microbiol Lett 204:381-5. 2001
  8. ncbi request reprint Heterologous overexpression of glucose dehydrogenase from the halophilic archaeon Haloferax mediterranei, an enzyme of the medium chain dehydrogenase/reductase family
    C Pire
    División de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Alicante, Ap 99, E 03080, Alicante, Spain
    FEMS Microbiol Lett 200:221-7. 2001
  9. ncbi request reprint Crystallization and preliminary X-ray analysis of glucose dehydrogenase from Haloferax mediterranei
    J Ferrer
    Departamento de Agroquimica y Bioquimica, Facultad de Ciencias, Universidad de Alicante, Ap 99, E 03080 Alicante, Spain
    Acta Crystallogr D Biol Crystallogr 57:1887-9. 2001
  10. ncbi request reprint Purification and characterisation of a possible assimilatory nitrite reductase from the halophile archaeon Haloferax mediterranei
    R M Martínez-Espinosa
    División de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Alicante, Ap 99, E 03080 Alicante, Spain
    FEMS Microbiol Lett 196:113-8. 2001

Collaborators

Detail Information

Publications13

  1. ncbi request reprint Glucose dehydrogenase from the halophilic Archaeon Haloferax mediterranei: enzyme purification, characterisation and N-terminal sequence
    M J Bonete
    División de Bioquímica, Facultad de Ciencias, Universidad de Alicante, Spain
    FEBS Lett 383:227-9. 1996
    ..However, the halophilic glucose dehydrogenase appears to be a dimeric protein, in contrast to the tetrameric enzyme from the thermophile...
  2. ncbi request reprint Occurrence of two different glutamate dehydrogenase activities in the halophilic bacterium Salinibacter ruber
    Maria Jose Bonete
    Division of Biochemistry and Molecular Biology, University of Alicante, 03080, Alicante, Spain
    FEMS Microbiol Lett 226:181-6. 2003
    ..A low-molecular-mass cytoplasmic fraction was found to be a highly effective activator of GDHII in the presence of high NaCl concentrations...
  3. pmc Biodiversity of Archaea and floral of two inland saltern ecosystems in the Alto Vinalopó Valley, Spain
    Basilio Zafrilla
    División de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Alicante, AP, 99, E 03080 Alicante, Spain
    Saline Systems 6:10. 2010
    ..However, studies focused on the characterization of inland solar salterns are scarce and both the archaeal diversity and the plant communities inhabiting these environments remain poorly described...
  4. ncbi request reprint 2-Hydroxyacid dehydrogenase from Haloferax mediterranei, a D-isomer-specific member of the 2-hydroxyacid dehydrogenase family
    M J Bonete
    Departamento de Bioquimica, Facultad de Ciencias, Universidad de Alicante, Ap 99, Alicante, Spain
    Biochimie 82:1143-50. 2000
    ..5 and 8.5. The optimum temperature was 52 degrees C and at 65 degrees C there was a pronounced activity decrease. This new enzyme can be used for the production of D-2-hydroxycarboxylic acid...
  5. ncbi request reprint Alpha-amylase activity from the halophilic archaeon Haloferax mediterranei
    F Perez-Pomares
    División de Bioquímica, Facultad de Ciencias, Universidad de Alicante, Apartado 99, 03080 Alicante, Spain
    Extremophiles 7:299-306. 2003
    ..The enzyme displayed maximal activity at pHs from 7 to 8, and its optimal temperature was in a range from 50 degrees C to 60 degrees C. The results also implicated several prototropic groups in the catalytic reaction...
  6. ncbi request reprint Analysis of acidic surface of Haloferax mediterranei glucose dehydrogenase by site-directed mutagenesis
    J Esclapez
    Departamento de Agroquimica y Bioquimica, División de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Alicante, Ap 99, E 03080 Alicante, Spain
    FEBS Lett 581:837-42. 2007
    ....
  7. ncbi request reprint Assimilatory nitrate reductase from the haloarchaeon Haloferax mediterranei: purification and characterisation
    R M Martínez-Espinosa
    División de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Alicante, Ap 99, E 03080, Alicante, Spain
    FEMS Microbiol Lett 204:381-5. 2001
    ..In contrast, NADPH and NADH, which are electron donors in nitrate reductases from different non-photosynthetic bacteria, were ineffective...
  8. ncbi request reprint Heterologous overexpression of glucose dehydrogenase from the halophilic archaeon Haloferax mediterranei, an enzyme of the medium chain dehydrogenase/reductase family
    C Pire
    División de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Alicante, Ap 99, E 03080, Alicante, Spain
    FEMS Microbiol Lett 200:221-7. 2001
    ..This method may be useful as a general procedure for purifying other halophilic proteins from mesophilic hosts...
  9. ncbi request reprint Crystallization and preliminary X-ray analysis of glucose dehydrogenase from Haloferax mediterranei
    J Ferrer
    Departamento de Agroquimica y Bioquimica, Facultad de Ciencias, Universidad de Alicante, Ap 99, E 03080 Alicante, Spain
    Acta Crystallogr D Biol Crystallogr 57:1887-9. 2001
    ..8, b = 110.9, c = 151.7 A, alpha = beta = gamma = 90 degrees. Calculated values for V(M) and consideration of the packing for both crystal forms suggests that the asymmetric units in both crystal forms contain a monomer...
  10. ncbi request reprint Purification and characterisation of a possible assimilatory nitrite reductase from the halophile archaeon Haloferax mediterranei
    R M Martínez-Espinosa
    División de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Alicante, Ap 99, E 03080 Alicante, Spain
    FEMS Microbiol Lett 196:113-8. 2001
    ..NADPH and NADH, which are electron donors in nitrite reductases from different non-photosynthetic bacteria, were not effective with nitrite reductase from H. mediterranei...
  11. ncbi request reprint Respiratory nitrate reductase from haloarchaeon Haloferax mediterranei: biochemical and genetic analysis
    B Lledó
    División de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Alicante, Ap 99, E 03080, Alicante, Spain
    Biochim Biophys Acta 1674:50-9. 2004
    ..The absorption spectrum indicated that Nar contains Fe-S clusters. Reverse transcriptase (RT-PCR) shows that regulation of nar genes occurs at transcriptional level induced by oxygen-limiting conditions and the presence of nitrate...
  12. ncbi request reprint Respiratory nitrate and nitrite pathway in the denitrifier haloarchaeon Haloferax mediterranei
    R M Martínez-Espinosa
    División de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Alicante, Campus de San Vicente del Raspeig, 03080 Alicante, Spain
    Biochem Soc Trans 34:115-7. 2006
    ..Nar has been purified and characterized in vitro. This characterization has included protein-film voltammetry and preliminary EPR studies...
  13. ncbi request reprint Fluorescence and quenching comparative studies of halophilic and bovine glutamate dehydrogenase
    J Ferrer
    División de Bioquímica, Facultad de Ciencias, Universidad de Alicante, Spain
    J Photochem Photobiol B 47:148-54. 1998
    ..The tryptophan residues in the protein are more exposed to the solvent in h-GDH than in b-GDH. The total amount of tryptophan residues is nearly the same for both enzymes...