Genomes and Genes
Affiliation: Insitute For Functional Biology and Genomics
Location: Salamanca, Spain
Basic research in mechanisms granting genome integrity.
- Regulation of CDC6, geminin, and CDT1 in human cells that undergo polyploidizationRodrigo Bermejo
Department of Biochemistry, Instituto de Investigaciones Biomedicas Alberto Sols, Universidad Autonoma de Madrid, CSIC, Arturo Duperier, 4 28029 Madrid, Spain
Mol Biol Cell 13:3989-4000. 2002..Our results show that both cdt1 and cdc6 are differentially regulated during megakaryocytic differentiation and suggest an active role of cdc6 in endomitosis...
- G-quadruplex-induced instability during leading-strand replicationJudith Lopes
Recombinaison et Instabilité Génétique, Institut Curie Centre de Recherche, CNRS UMR3244, Universite Pierre et Marie Curie, Paris Cedex 05, France
EMBO J 30:4033-46. 2011..The asymmetrical behaviour of G-quadruplex prone sequences during replication has implications for their evolutionary dynamics within genomes, including the maintenance of G-rich telomeres...
- The replication checkpoint protects fork stability by releasing transcribed genes from nuclear poresRodrigo Bermejo
Fondazione Istituto FIRC di Oncologia Molecolare IFOM at IFOM IEO Campus, Via Adamello 16, 20139 Milan, Italy
Cell 146:233-46. 2011....
- Genome-wide function of THO/TREX in active genes prevents R-loop-dependent replication obstaclesBelén Gómez-González
Centro Andaluz de Biologia Molecular y Medicina Regenerativa CABIMER, Universidad de Sevilla CSIC, Sevilla, Spain
EMBO J 30:3106-19. 2011..Our work establishes a genome-wide function for THO-Sub2 in transcription elongation and mRNP biogenesis that function to prevent the accumulation of transcription-mediated replication obstacles, including R-loops...
- Replication termination at eukaryotic chromosomes is mediated by Top2 and occurs at genomic loci containing pausing elementsDaniele Fachinetti
Fondazione IFOM, Istituto FIRC di Oncologia Molecolare IFOM IEO Campus, Via Adamello 16, 20139 Milan, Italy
Mol Cell 39:595-605. 2010..We propose that in eukaryotes, replication fork barriers, Rrm3, and Top2 coordinate replication fork progression and fusion at TERs, thus counteracting abnormal genomic transitions...
- ChIP-on-chip analysis of DNA topoisomerasesRodrigo Bermejo
Fondazione Italiana per la Ricerca sul Cancro, Institute of Molecular Oncology Foundation, Milan, Italy
Methods Mol Biol 582:103-18. 2009..By using this approach, novel aspects of DNA topoisomerase function in chromosome metabolism might be unmasked...
- Genome-organizing factors Top2 and Hmo1 prevent chromosome fragility at sites of S phase transcriptionRodrigo Bermejo
Fondazione IFOM Istituto FIRC di Oncologia Moleculare IFOM IEO Campus, Via Adamello 16, 20139 Milan, Italy
Cell 138:870-84. 2009..We propose that an Hmo1-dependent epigenetic signature together with Top2 mediate an S phase architectural pathway to preserve genome integrity...
- Replicon dynamics, dormant origin firing, and terminal fork integrity after double-strand break formationYlli Doksani
FIRC Institute of Molecular Oncology Foundation IFOM IEO Campus and DSBB Università degli Studi di Milano, Via Adamello 16, 20139 Milan, Italy
Cell 137:247-58. 2009..Our findings have implications for those genome instability syndromes that accumulate DNA breaks during S phase and for forks encountering eroding telomeres...
- SRS2 and SGS1 prevent chromosomal breaks and stabilize triplet repeats by restraining recombinationAlix Kerrest
Institut Pasteur, Unité de Génétique Moléculaire des Levures, CNRS, URA2171, Universite Pierre et Marie Curie, UFR 927, 25 rue du Dr Roux, F 75015 Paris, France
Nat Struct Mol Biol 16:159-67. 2009..In the absence of Srs2 or Sgs1, DNA damage accumulates and is processed by homologous recombination, triggering repeat rearrangements...
- Cohesion by topology: sister chromatids interlocked by DNARodrigo Bermejo
Fondazione Italiana per la Ricerca sul Cancro FIRC Institute of Molecular Oncology Foundation, 20139 Milan, Italy
Genes Dev 22:2297-301. 2008..They propose that DNA precatenanes, arising during replication fork progression, hold sister chromatids together...
- Top1- and Top2-mediated topological transitions at replication forks ensure fork progression and stability and prevent DNA damage checkpoint activationRodrigo Bermejo
Fondazione Italiana per la Ricerca sul Cancro FIRC, Institute of Molecular Oncology Foundation IFOM IEO Campus and DSBB Università degli Studi di Milano, 20139 Milan, Italy
Genes Dev 21:1921-36. 2007..A failure in resolving fork-related topological constrains during S phase may therefore result in abnormal chromosome transitions, DNA damage checkpoint activation, and chromosome breakage during segregation...
- A novel E2 box-GATA element modulates Cdc6 transcription during human cells polyploidizationNuria Vilaboa
Department of Biochemistry, Instituto de Investigaciones Biomédicas A Sols, Universidad Autonoma CSIC, Arturo Duperier, 4, 28029 Madrid, Spain
Nucleic Acids Res 32:6454-67. 2004..Altogether, these data suggest that cdc6 expression could be actively maintained during megakaryocytic differentiation through transcriptional mechanisms involving specific cis- and trans-regulatory elements...
- The DNA damage checkpoint response to replication stress: A Game of ForksRachel Jossen
Instituto de Biología Funcional y Genómica, CSIC USAL Salamanca, Spain
Front Genet 4:26. 2013..We also highlight the checkpoint function as an anti-cancer barrier preventing cells malignant transformation following oncogene-induced replication stress...
- Preserving the genome by regulating chromatin association with the nuclear envelopeRodrigo Bermejo
IFOM Fondazione Istituto FIRC di Oncologia Molecolare at the IFOM European Institute of Oncology Campus, Via Adamello 16, 20139 Milan, Italy
Trends Cell Biol 22:465-73. 2012..Here, we review the interplay between the nuclear envelope, chromatin and DNA damage checkpoint pathways, and discuss the physiological and pathological implications of these associations...
- Preventing replication stress to maintain genome stability: resolving conflicts between replication and transcriptionRodrigo Bermejo
Fondazione Istituto FIRC di Oncologia Molecolare, at IFOM IEO Campus Via Adamello 16, 20139 Milan, Italy
Mol Cell 45:710-8. 2012..The evolutionary implications of such conflicts on genome dynamics and their potential impact on oncogenic stress are discussed...