Gustavo Barja

Summary

Affiliation: Universidad Complutense
Country: Spain

Publications

  1. ncbi Free radicals and aging
    Gustavo Barja
    Department of Animal Physiology II, Faculty of Biology, Complutense University, Madrid 28040, Spain
    Trends Neurosci 27:595-600. 2004
  2. ncbi Effect of graded corticosterone treatment on aging-related markers of oxidative stress in rat liver mitochondria
    Pilar Caro
    Department of Animal Physiology II, Facultad de Ciencias Biologicas, Complutense University, c Antonio Novais 2, Madrid 28040, Spain
    Biogerontology 8:1-11. 2007
  3. ncbi Mitochondrial oxygen consumption and reactive oxygen species production are independently modulated: implications for aging studies
    Gustavo Barja
    Department of Animal Physiology II, Faculty of Biological Sciences, Complutense University, c Antonio Novais 2, Madrid 28040, Spain
    Rejuvenation Res 10:215-24. 2007
  4. ncbi Rate of generation of oxidative stress-related damage and animal longevity
    Gustavo Barja
    Department of Animal Biology II Animal Physiology, Faculty of Biology, Complutense University, Madrid 28040, Spain
    Free Radic Biol Med 33:1167-72. 2002
  5. ncbi The gene cluster hypothesis of aging and longevity
    Gustavo Barja
    Departamento de Fisiología Animal II, Facultad de Ciencias Biologicas, Universidad Complutense, Madrid, Spain
    Biogerontology 9:57-66. 2008
  6. doi Forty percent methionine restriction decreases mitochondrial oxygen radical production and leak at complex I during forward electron flow and lowers oxidative damage to proteins and mitochondrial DNA in rat kidney and brain mitochondria
    Pilar Caro
    Department of Animal Physiology II, Complutense University of Madrid, Madrid, Spain
    Rejuvenation Res 12:421-34. 2009
  7. ncbi Effect of methionine dietary supplementation on mitochondrial oxygen radical generation and oxidative DNA damage in rat liver and heart
    Jose Gomez
    Department of Animal Physiology II, Faculty of Biological Sciences, Complutense University, Madrid, Spain
    J Bioenerg Biomembr 41:309-21. 2009
  8. doi Forty percent methionine restriction lowers DNA methylation, complex I ROS generation, and oxidative damage to mtDNA and mitochondrial proteins in rat heart
    Ines Sanchez-Roman
    Department of Animal Physiology II, Faculty of Biological Sciences, Complutense University of Madrid UCM, Madrid, Spain
    J Bioenerg Biomembr 43:699-708. 2011
  9. doi Effect of 40% restriction of dietary amino acids (except methionine) on mitochondrial oxidative stress and biogenesis, AIF and SIRT1 in rat liver
    Pilar Caro
    Departamento de Fisiología Animal II, Universidad Complutense, Madrid, Spain
    Biogerontology 10:579-92. 2009
  10. doi The β-blocker atenolol lowers the longevity-related degree of fatty acid unsaturation, decreases protein oxidative damage, and increases extracellular signal-regulated kinase signaling in the heart of C57BL/6 mice
    Ines Sanchez-Roman
    Department of Animal Physiology II, Faculty of Biological Sciences, Complutense University, Madrid, Spain
    Rejuvenation Res 13:683-93. 2010

Collaborators

Detail Information

Publications46

  1. ncbi Free radicals and aging
    Gustavo Barja
    Department of Animal Physiology II, Faculty of Biology, Complutense University, Madrid 28040, Spain
    Trends Neurosci 27:595-600. 2004
    ..Furthermore, caloric restriction, which decreases the rate of aging, proportionately decreases mitochondrial oxygen radical generation, especially at complex I. These findings are reviewed, highlighting the results obtained in the brain...
  2. ncbi Effect of graded corticosterone treatment on aging-related markers of oxidative stress in rat liver mitochondria
    Pilar Caro
    Department of Animal Physiology II, Facultad de Ciencias Biologicas, Complutense University, c Antonio Novais 2, Madrid 28040, Spain
    Biogerontology 8:1-11. 2007
    ..However, they show that this hormone modulates the level of oxidative stress both in proteins and in mtDNA. Some of these changes can contribute to the chronic effects of the hormone at tissue level...
  3. ncbi Mitochondrial oxygen consumption and reactive oxygen species production are independently modulated: implications for aging studies
    Gustavo Barja
    Department of Animal Physiology II, Faculty of Biological Sciences, Complutense University, c Antonio Novais 2, Madrid 28040, Spain
    Rejuvenation Res 10:215-24. 2007
    ..Thus, the apparently paradoxical increases in O2 consumption observed in some models of dietary restriction do not discredit the mitochondrial free radical theory of aging, and they can further strengthen it...
  4. ncbi Rate of generation of oxidative stress-related damage and animal longevity
    Gustavo Barja
    Department of Animal Biology II Animal Physiology, Faculty of Biology, Complutense University, Madrid 28040, Spain
    Free Radic Biol Med 33:1167-72. 2002
    ..These two traits suggest that the rate of generation of endogenous oxidative damage determines, at least in part, the rate of aging in animals...
  5. ncbi The gene cluster hypothesis of aging and longevity
    Gustavo Barja
    Departamento de Fisiología Animal II, Facultad de Ciencias Biologicas, Universidad Complutense, Madrid, Spain
    Biogerontology 9:57-66. 2008
    ..The huge benefits that could be obtained discovering the longevity gene cluster will amply outweigh the comparatively small research effort involved...
  6. doi Forty percent methionine restriction decreases mitochondrial oxygen radical production and leak at complex I during forward electron flow and lowers oxidative damage to proteins and mitochondrial DNA in rat kidney and brain mitochondria
    Pilar Caro
    Department of Animal Physiology II, Complutense University of Madrid, Madrid, Spain
    Rejuvenation Res 12:421-34. 2009
    ..We conclude that methionine is the only dietary factor responsible for the decrease in mitochondrial ROS production and oxidative stress, and likely for part of the longevity extension effect, occurring in DR...
  7. ncbi Effect of methionine dietary supplementation on mitochondrial oxygen radical generation and oxidative DNA damage in rat liver and heart
    Jose Gomez
    Department of Animal Physiology II, Faculty of Biological Sciences, Complutense University, Madrid, Spain
    J Bioenerg Biomembr 41:309-21. 2009
    ..These results show that methionine supplementation in the diet specifically increases mitochondrial ROS production and mitochondrial DNA oxidative damage in rat liver mitochondria offering a plausible mechanism for its hepatotoxicity...
  8. doi Forty percent methionine restriction lowers DNA methylation, complex I ROS generation, and oxidative damage to mtDNA and mitochondrial proteins in rat heart
    Ines Sanchez-Roman
    Department of Animal Physiology II, Faculty of Biological Sciences, Complutense University of Madrid UCM, Madrid, Spain
    J Bioenerg Biomembr 43:699-708. 2011
    ..They also highlight some of the mechanisms involved in the generation of these beneficial effects...
  9. doi Effect of 40% restriction of dietary amino acids (except methionine) on mitochondrial oxidative stress and biogenesis, AIF and SIRT1 in rat liver
    Pilar Caro
    Departamento de Fisiología Animal II, Universidad Complutense, Madrid, Spain
    Biogerontology 10:579-92. 2009
    ..They also show for the first time that restriction of dietary amino acids different from methionine decreases mitochondrial protein oxidative modification and AIF, and increases SIRT1, in rat liver...
  10. doi The β-blocker atenolol lowers the longevity-related degree of fatty acid unsaturation, decreases protein oxidative damage, and increases extracellular signal-regulated kinase signaling in the heart of C57BL/6 mice
    Ines Sanchez-Roman
    Department of Animal Physiology II, Faculty of Biological Sciences, Complutense University, Madrid, Spain
    Rejuvenation Res 13:683-93. 2010
    ....
  11. doi Effect of every other day feeding on mitochondrial free radical production and oxidative stress in mouse liver
    Pilar Caro
    Department of Animal Physiology II, Complutense University, Madrid, Spain
    Rejuvenation Res 11:621-9. 2008
    ..These results support the possibility that EOD DR increases maximum life span at least in part through decreases in mitochondrial oxidative stress which are independent from insulin/IGF-1-like signaling...
  12. ncbi Effect of 8.5% and 25% caloric restriction on mitochondrial free radical production and oxidative stress in rat liver
    Jose Gomez
    Department of Animal Physiology II, Complutense University, c Antonio Novais 2, Madrid 28040, Spain
    Biogerontology 8:555-66. 2007
    ....
  13. doi Effects of aging and methionine restriction applied at old age on ROS generation and oxidative damage in rat liver mitochondria
    Ines Sanchez-Roman
    Department of Animal Physiology II, Faculty of Biological Sciences, Complutense University of Madrid UCM, c Jose Antonio Novais 2, Madrid, Spain
    Biogerontology 13:399-411. 2012
    ..Aged rats maintain the capacity to lower mitochondrial ROS generation and oxidative stress in response to a short-term exposure to restriction of a single dietary substance: methionine...
  14. ncbi Methionine restriction decreases mitochondrial oxygen radical generation and leak as well as oxidative damage to mitochondrial DNA and proteins
    Alberto Sanz
    Department of Animal Physiology II, Complutense University, Madrid, Spain
    FASEB J 20:1064-73. 2006
    ....
  15. ncbi Testing the vicious cycle theory of mitochondrial ROS production: effects of H2O2 and cumene hydroperoxide treatment on heart mitochondria
    Alberto Sanz
    Department of Animal Physiology II, Faculty of Biology, Complutense University, Madrid, 28040, Spain
    J Bioenerg Biomembr 38:121-7. 2006
    ....
  16. doi Forty percent and eighty percent methionine restriction decrease mitochondrial ROS generation and oxidative stress in rat liver
    Pilar Caro
    Departamento de Fisiología Animal II, Facultad de Ciencias Biologicas, Complutense University, c Jose Antonio Novais 2, Madrid 28040, Spain
    Biogerontology 9:183-96. 2008
    ..The results show that 40% isocaloric MetR is enough to decrease ROS production and oxidative stress in rat liver. This suggests that the lowered intake of methionine is responsible for the decrease in oxidative stress observed in DR...
  17. ncbi Short-term caloric restriction and sites of oxygen radical generation in kidney and skeletal muscle mitochondria
    Ricardo Gredilla
    Department of Animal Biology II Animal Physiology, Faculty of Biology, Complutense University, 28040 Madrid, Spain
    Ann N Y Acad Sci 1019:333-42. 2004
    ....
  18. ncbi Effect of lipid restriction on mitochondrial free radical production and oxidative DNA damage
    Alberto Sanz
    Department of Animal Physiology II, Faculty of Biological Sciences, Complutense University, Madrid, Spain
    Ann N Y Acad Sci 1067:200-9. 2006
    ..These results deny a role for lipids and reinforce the possible role of dietary proteins as being responsible for the decrease in mitochondrial ROS production and DNA damage in caloric restriction...
  19. doi Regulation of longevity and oxidative stress by nutritional interventions: role of methionine restriction
    Ines Sanchez-Roman
    Department of Animal Physiology II, Faculty of Biological Sciences, Complutense University of Madrid UCM, Spain
    Exp Gerontol 48:1030-42. 2013
    ..This would decrease the accumulation rate of somatic mutations in mtDNA and maybe finally also in nuclear DNA. ..
  20. doi [Effect of restricting amino acids except methionine on mitochondrial oxidative stress]
    Pilar Caro
    Departamento de Fisiología Animal II, Facultad de Ciencias Biologicas, Universidad Complutense de Madrid, Espana
    Rev Esp Geriatr Gerontol 44:194-9. 2009
    ..To clarify this question, we studied the effect of restricting all the amino acids, except methionine, of the semi-purified diet, AIN 93G, in Wistar rats...
  21. ncbi Aging in vertebrates, and the effect of caloric restriction: a mitochondrial free radical production-DNA damage mechanism?
    Gustavo Barja
    Department of Animal Biology II Animal Physiology, Faculty of Biology, Complutense University, Madrid 28040, Spain
    Biol Rev Camb Philos Soc 79:235-51. 2004
    ..It is suggested that a low rate of mitochondrial ROS generation extends lifespan both in long-lived and in caloric-restricted animals by determining the rate of oxidative attack and accumulation of somatic mutations in mitochondrial DNA...
  22. pmc Evaluation of sex differences on mitochondrial bioenergetics and apoptosis in mice
    Alberto Sanz
    Department of Animal Physiology II, Faculty of Biology, Complutense University, Madrid 28040, Spain
    Exp Gerontol 42:173-82. 2007
    ..These results support the Mitochondrial Free Radical Theory of Aging, and indicate that oxidative stress generation independent of estrogen levels determines aging rate...
  23. ncbi Is the mitochondrial free radical theory of aging intact?
    Alberto Sanz
    Department of Animal Physiology II, Faculty of Biological Sciences, Complutense University, Madrid, Spain
    Antioxid Redox Signal 8:582-99. 2006
    ..Taken together, available information is consistent with the predictions of the mitochondrial free radical theory of aging, although definitive proof and many mechanistic details are still lacking...
  24. ncbi Dietary restriction at old age lowers mitochondrial oxygen radical production and leak at complex I and oxidative DNA damage in rat brain
    Alberto Sanz
    Department of Animal Physiology II, Faculty of Biological Sciences, Complutense University, c Antonio Novais 2, Madrid 28040, Spain
    J Bioenerg Biomembr 37:83-90. 2005
    ..The results agree with the idea that CR decreases aging rate in part by lowering the rate of free radical generation of mitochondria in the brain...
  25. ncbi [Calorie restriction, oxidative stress and longevity]
    Monica Lopez-Torres
    Departamento de Fisiología Animal II, Facultad de Ciencias Biologicas, Universidad Complutense de Madrid, Madrid, Espana
    Rev Esp Geriatr Gerontol 43:252-60. 2008
    ..Greater longevity would be partly achieved by a low rate of endogenous oxidative damage generation, but also by a macromolecular composition highly resistant to oxidative modification, as is the case for lipids and proteins...
  26. ncbi Independent and additive effects of atenolol and methionine restriction on lowering rat heart mitochondria oxidative stress
    Ines Sanchez-Roman
    Department of Animal Physiology II, Faculty of Biological Sciences, Complutense University of Madrid UCM, Madrid, Spain
    J Bioenerg Biomembr 46:159-72. 2014
    ....
  27. ncbi Methionine and homocysteine modulate the rate of ROS generation of isolated mitochondria in vitro
    Jose Gomez
    Department of Animal Physiology II, Faculty of Biological Sciences, Complutense University, Madrid, Spain
    J Bioenerg Biomembr 43:377-86. 2011
    ..The variations in ROS production are localized at complexes I or III. These changes add to previously described chronic effects of methionine restriction and supplementation in vivo...
  28. ncbi Protein restriction without strong caloric restriction decreases mitochondrial oxygen radical production and oxidative DNA damage in rat liver
    Alberto Sanz
    Department of Animal Physiology II, Faculty of Biological Sciences, Complutense University, Madrid, 28040, Spain
    J Bioenerg Biomembr 36:545-52. 2004
    ..Interestingly, these tissue oxidative stress-linked parameters can be lowered by restricting only the intake of dietary protein, probably a more feasible option than caloric restriction for adult humans...
  29. pmc Updating the mitochondrial free radical theory of aging: an integrated view, key aspects, and confounding concepts
    Gustavo Barja
    Department of Animal Physiology II, Faculty of Biological Sciences, Complutense University, Madrid, Spain
    Antioxid Redox Signal 19:1420-45. 2013
    ....
  30. ncbi Lifelong treatment with atenolol decreases membrane fatty acid unsaturation and oxidative stress in heart and skeletal muscle mitochondria and improves immunity and behavior, without changing mice longevity
    Alexia Gomez
    Department of Animal Physiology II, Faculty of Biological Sciences, Complutense University of Madrid UCM, Madrid, Spain
    Aging Cell 13:551-60. 2014
    ..We conclude that it is atenolol that failed to increase longevity, and likely not the decrease in membrane unsaturation induced by the drug...
  31. ncbi Lowered methionine ingestion as responsible for the decrease in rodent mitochondrial oxidative stress in protein and dietary restriction possible implications for humans
    Monica Lopez-Torres
    Department of Animal Physiology II, Faculty of Biological Sciences, Complutense University, Madrid 28040, Spain
    Biochim Biophys Acta 1780:1337-47. 2008
    ..Therefore, decreasing such levels to the recommended ones has a great potential to lower tissue oxidative stress and to increase healthy life span in humans while avoiding the possible undesirable effects of DR diets...
  32. ncbi Influence of aging and long-term caloric restriction on oxygen radical generation and oxidative DNA damage in rat liver mitochondria
    Monica Lopez-Torres
    Department of Animal Biology II Animal Physiology, Faculty of Biology, Complutense University, Madrid, Spain
    Free Radic Biol Med 32:882-9. 2002
    ..These results support the idea that caloric restriction reduces the aging rate at least in part by decreasing the rate of mitochondrial ROS production and so, the rate of oxidative attack to biological macromolecules like mtDNA...
  33. doi Mitochondrial DNA sequences are present inside nuclear DNA in rat tissues and increase with age
    Pilar Caro
    Department of Animal Physiology II, Complutense University of Madrid, Spain
    Mitochondrion 10:479-86. 2010
    ..Bone marrow metaphase cells from both young and old rats show mtDNA at centromeric regions in 20 out of the 2n=40 chromosomes. Consequently, mitochondria can be a major trigger of aging but the final target could also be the nucleus...
  34. ncbi Minireview: the role of oxidative stress in relation to caloric restriction and longevity
    Ricardo Gredilla
    Department of Animal Physiology II, Faculty of Biology, Complutense University, Madrid, Spain
    Endocrinology 146:3713-7. 2005
    ..In this study we review recent reports of caloric restriction and longevity, focusing on mitochondrial oxidative stress and the proposed mechanisms leading to an extended longevity in calorie-restricted animals...
  35. ncbi The quantitative measurement of H2O2 generation in isolated mitochondria
    Gustavo Barja
    Department of Animal Biology II Animal Physiology, Faculty of Biology, Complutense Univesity, Madrid, Spain
    J Bioenerg Biomembr 34:227-33. 2002
    ..Its application to the localization of the sites where caloric restriction decreases mitochondrial oxygen radical generation in heart mitochondria is described...
  36. ncbi Endogenous oxidative stress: relationship to aging, longevity and caloric restriction
    Gustavo Barja
    Department of Animal Biology II Animal Physiology, Faculty of Biology, Complutense University, 28040, Madrid, Spain
    Ageing Res Rev 1:397-411. 2002
    ..These results suggest that similar mechanisms have been used to extend longevity through decreases in oxidative stress in caloric restriction and during the evolution of species with different longevities...
  37. ncbi Mitochondrial oxidative stress, aging and caloric restriction: the protein and methionine connection
    Reinald Pamplona
    Department of Basic Medical Sciences, University of Lleida, Lleida 25008, Spain
    Biochim Biophys Acta 1757:496-508. 2006
    ....
  38. ncbi Membrane fatty acid unsaturation, protection against oxidative stress, and maximum life span: a homeoviscous-longevity adaptation?
    Reinald Pamplona
    Metabolic Physiopathology Research Group, Department of Basic Medical Sciences, Faculty of Medicine, University of Lleida, Lleida 25198, Spain
    Ann N Y Acad Sci 959:475-90. 2002
    ..Here, the physiological meaning of these findings and the effects of experimental manipulations such as dietary stress, caloric restriction, and endocrine control in relation to aging and longevity are discussed...
  39. ncbi Modification of the longevity-related degree of fatty acid unsaturation modulates oxidative damage to proteins and mitochondrial DNA in liver and brain
    Reinald Pamplona
    Department of Basic Medical Sciences, Faculty of Medicine, University of Lleida, Lleida 25198, Spain
    Exp Gerontol 39:725-33. 2004
    ..This is mechanistically relevant in relation to the constitutively low tissue fatty acid unsaturation of long-lived animals...
  40. ncbi Highly resistant macromolecular components and low rate of generation of endogenous damage: two key traits of longevity
    Reinald Pamplona
    Department of Basic Medical Sciences, Faculty of Medicine, University of Lleida, Lleida 25008, Spain
    Ageing Res Rev 6:189-210. 2007
    ..It is emphasized that both a low rate of generation of endogenous damage and an intrinsically high resistance to modification of tissue macromolecules are key traits of animal longevity...
  41. ncbi Aging increases Nepsilon-(carboxymethyl)lysine and caloric restriction decreases Nepsilon-(carboxyethyl)lysine and Nepsilon-(malondialdehyde)lysine in rat heart mitochondrial proteins
    Reinald Pamplona
    Department of Basic Medical Sciences, Faculty of Medicine, Lleida University, Spain
    Free Radic Res 36:47-54. 2002
    ..The CEL and MDA-lys results support the notion that caloric restriction decreases oxidative stress-derived damage to heart mitochondrial proteins...
  42. ncbi Methionine restriction decreases endogenous oxidative molecular damage and increases mitochondrial biogenesis and uncoupling protein 4 in rat brain
    Alba Naudi
    Department of Experimental Medicine, Faculty of Medicine, University of Lleida IRBLleida, c Montserrat Roig 2, Lleida, Spain
    Rejuvenation Res 10:473-84. 2007
    ..These beneficial MetR-induced changes likely derived from metabolic reprogramming at the cellular and tissue level can play a key role in the protection against aging-associated neurodegenerative disorders...
  43. ncbi Oxidative, glycoxidative and lipoxidative damage to rat heart mitochondrial proteins is lower after 4 months of caloric restriction than in age-matched controls
    Reinald Pamplona
    Department of Basic Medical Sciences, Faculty of Medicine, Lleida University, Lleida 25198, Spain
    Mech Ageing Dev 123:1437-46. 2002
    ..They also suggest that these decreases are due to an increase in the capacity of the restricted mitochondria to decompose oxidatively modified proteins...
  44. ncbi Dietary protein restriction decreases oxidative protein damage, peroxidizability index, and mitochondrial complex I content in rat liver
    Victoria Ayala
    Department of Basic Medical Sciences, Faculty of Medicine, University of Lleida, Spain
    J Gerontol A Biol Sci Med Sci 62:352-60. 2007
    ..Recent studies suggest that those benefits of PR could be caused, in turn, by the lowered methionine intake of that dietary manipulation...
  45. ncbi Short-term caloric restriction and regulatory proteins of apoptosis in heart, skeletal muscle and kidney of Fischer 344 rats
    Colin Selman
    University of Florida, Biochemistry of Aging Laboratory, College of Health and Human Performance, Box 118206, Gainesville, FL 32611, USA
    Biogerontology 4:141-7. 2003
    ....
  46. ncbi Protein methionine content and MDA-lysine adducts are inversely related to maximum life span in the heart of mammals
    Maria Cristina Ruiz
    Department of Basic Medical Sciences, University of Lleida, Lleida 25198, Spain
    Mech Ageing Dev 126:1106-14. 2005
    ..This is obtained by modifying the constituent structural components of proteins and lipids, selecting those less sensitive to oxidative modifications...