Andres Aguilera

Summary

Affiliation: Universidad de Sevilla
Country: Spain

Publications

  1. doi R loops: from transcription byproducts to threats to genome stability
    Andres Aguilera
    CABIMER, Universidad de Sevilla CSIC, Av Americo Vespucio s n, 41092 Sevilla, Spain
    Mol Cell 46:115-24. 2012
  2. pmc AID induces double-strand breaks at immunoglobulin switch regions and c-MYC causing chromosomal translocations in yeast THO mutants
    Jose F Ruiz
    Centro Andaluz de Biologia Molecular y Medicina Regenerativa CABIMER, Universidad de Sevilla CSIC, Sevilla, Spain
    PLoS Genet 7:e1002009. 2011
  3. pmc Topological constraints impair RNA polymerase II transcription and causes instability of plasmid-borne convergent genes
    María L García-Rubio
    Centro Andaluz de Biologia Molecular y Medicina Regenerativa CABIMER, Universidad de Sevilla CSIC, Av Américo Vespucio s n 41092 Sevilla, and Departamento de Genética, Universidad de Sevilla, 41012 Sevilla, Spain
    Nucleic Acids Res 40:1050-64. 2012
  4. pmc Complex chromosomal rearrangements mediated by break-induced replication involve structure-selective endonucleases
    Benjamin Pardo
    Centro Andaluz de Biologia Molecular y Medicina Regenerativa CABIMER, Universidad de Sevilla, Sevilla, Spain
    PLoS Genet 8:e1002979. 2012
  5. pmc Competing roles of DNA end resection and non-homologous end joining functions in the repair of replication-born double-strand breaks by sister-chromatid recombination
    Sandra Muñoz-Galván
    Centro Andaluz de Biologia Molecular y Medicina Regenerativa CABIMER, Universidad de Sevilla CSIC, Av Americo Vespucio s n, 41092 Seville, Spain
    Nucleic Acids Res 41:1669-83. 2013
  6. pmc Different genetic requirements for repair of replication-born double-strand breaks by sister-chromatid recombination and break-induced replication
    Felipe Cortes-Ledesma
    Departamento de Genetica, Facultad de Biologia, Universidad de Sevilla, and Departamento de Biología Molecular, CABIMER, CSIC Universidad de Sevilla, Av Americo Vespucio s n, 41092 Sevilla, Spain
    Nucleic Acids Res 35:6560-70. 2007
  7. pmc The THO complex as a key mRNP biogenesis factor in development and cell differentiation
    Sonia Jimeno
    Centro Andaluz de Biologia Molecular y Medicina Regenerativa, Av Americo Vespucio s n, 41092 Sevilla, Spain
    J Biol 9:6. 2010
  8. pmc R-loop mediated transcription-associated recombination in trf4Δ mutants reveals new links between RNA surveillance and genome integrity
    Sandra Gavaldá
    Departamento de Biologia Molecular, Centro Andaluz de Biologia Molecular y Medicina Regenerativa CABIMER, Universidad de Sevilla, Seville, Spain
    PLoS ONE 8:e65541. 2013
  9. pmc A new connection of mRNP biogenesis and export with transcription-coupled repair
    Hélène Gaillard
    Departamento de Genetica, Facultad de Biologia, Universidad de Sevilla, CABIMER, CSIC Universidad de Sevilla, Avenida Americo Vespucio s n, 41092 Sevilla, Spain
    Nucleic Acids Res 35:3893-906. 2007
  10. pmc Cleavage factor I links transcription termination to DNA damage response and genome integrity maintenance in Saccharomyces cerevisiae
    Hélène Gaillard
    Centro Andaluz de Biologia Molecular y Medicina Regenerativa CABIMER, Universidad de Sevilla CSIC, Sevilla, Spain
    PLoS Genet 10:e1004203. 2014

Collaborators

Detail Information

Publications71

  1. doi R loops: from transcription byproducts to threats to genome stability
    Andres Aguilera
    CABIMER, Universidad de Sevilla CSIC, Av Americo Vespucio s n, 41092 Sevilla, Spain
    Mol Cell 46:115-24. 2012
    ..Here we discuss the factors and cellular processes that control R loop formation and the mechanisms by which R loops may influence gene expression and the integrity of the genome...
  2. pmc AID induces double-strand breaks at immunoglobulin switch regions and c-MYC causing chromosomal translocations in yeast THO mutants
    Jose F Ruiz
    Centro Andaluz de Biologia Molecular y Medicina Regenerativa CABIMER, Universidad de Sevilla CSIC, Sevilla, Spain
    PLoS Genet 7:e1002009. 2011
    ....
  3. pmc Topological constraints impair RNA polymerase II transcription and causes instability of plasmid-borne convergent genes
    María L García-Rubio
    Centro Andaluz de Biologia Molecular y Medicina Regenerativa CABIMER, Universidad de Sevilla CSIC, Av Américo Vespucio s n 41092 Sevilla, and Departamento de Genética, Universidad de Sevilla, 41012 Sevilla, Spain
    Nucleic Acids Res 40:1050-64. 2012
    ..Our work shows that topological constraints negatively affect RNAPII transcription and genetic integrity, and provides an assay to study gene regulation by transcription interference...
  4. pmc Complex chromosomal rearrangements mediated by break-induced replication involve structure-selective endonucleases
    Benjamin Pardo
    Centro Andaluz de Biologia Molecular y Medicina Regenerativa CABIMER, Universidad de Sevilla, Sevilla, Spain
    PLoS Genet 8:e1002979. 2012
    ..Altogether, our study provides evidence for a role of SSEs at multiple steps during BIR, thus participating in the destabilization of the genome by generating complex chromosomal rearrangements...
  5. pmc Competing roles of DNA end resection and non-homologous end joining functions in the repair of replication-born double-strand breaks by sister-chromatid recombination
    Sandra Muñoz-Galván
    Centro Andaluz de Biologia Molecular y Medicina Regenerativa CABIMER, Universidad de Sevilla CSIC, Av Americo Vespucio s n, 41092 Seville, Spain
    Nucleic Acids Res 41:1669-83. 2013
    ..These results further our understanding of the role of DNA resection in repair of replication-born DSBs revealing unanticipated differences between these events and repair of enzymatically induced DSBs...
  6. pmc Different genetic requirements for repair of replication-born double-strand breaks by sister-chromatid recombination and break-induced replication
    Felipe Cortes-Ledesma
    Departamento de Genetica, Facultad de Biologia, Universidad de Sevilla, and Departamento de Biología Molecular, CABIMER, CSIC Universidad de Sevilla, Av Americo Vespucio s n, 41092 Sevilla, Spain
    Nucleic Acids Res 35:6560-70. 2007
    ..Our study provides molecular evidence for inversions between repeats occurring by BIR followed by single-strand annealing (SSA) in the absence of strand exchange...
  7. pmc The THO complex as a key mRNP biogenesis factor in development and cell differentiation
    Sonia Jimeno
    Centro Andaluz de Biologia Molecular y Medicina Regenerativa, Av Americo Vespucio s n, 41092 Sevilla, Spain
    J Biol 9:6. 2010
    ..A recent study in BMC Biology on the role of the THOC5 subunit in cell physiology and mouse development provides new clues to the role of the THO complex in cell differentiation...
  8. pmc R-loop mediated transcription-associated recombination in trf4Δ mutants reveals new links between RNA surveillance and genome integrity
    Sandra Gavaldá
    Departamento de Biologia Molecular, Centro Andaluz de Biologia Molecular y Medicina Regenerativa CABIMER, Universidad de Sevilla, Seville, Spain
    PLoS ONE 8:e65541. 2013
    ..Altogether our data indicate that Trf4 is necessary for both mRNA surveillance and maintenance of genome integrity, serving as a link between RNA and DNA metabolism in S. cerevisiae...
  9. pmc A new connection of mRNP biogenesis and export with transcription-coupled repair
    Hélène Gaillard
    Departamento de Genetica, Facultad de Biologia, Universidad de Sevilla, CABIMER, CSIC Universidad de Sevilla, Avenida Americo Vespucio s n, 41092 Sevilla, Spain
    Nucleic Acids Res 35:3893-906. 2007
    ..Our results indicate that RNAPII is not proficient for TCR in mRNP biogenesis and export mutants, opening new perspectives on our knowledge of TCR in eukaryotic cells...
  10. pmc Cleavage factor I links transcription termination to DNA damage response and genome integrity maintenance in Saccharomyces cerevisiae
    Hélène Gaillard
    Centro Andaluz de Biologia Molecular y Medicina Regenerativa CABIMER, Universidad de Sevilla CSIC, Sevilla, Spain
    PLoS Genet 10:e1004203. 2014
    ..These findings reveal a new function for CFI in the DDR and underscore the importance of coordinating transcription termination with replication in the maintenance of genomic stability. ..
  11. pmc Genome-wide analysis of factors affecting transcription elongation and DNA repair: a new role for PAF and Ccr4-not in transcription-coupled repair
    Hélène Gaillard
    Centro Andaluz de Biologia Molecular y Medicina Regenerativa CABIMER, Universidad de Sevilla CSIC, Sevilla, Spain
    PLoS Genet 5:e1000364. 2009
    ....
  12. pmc Genome instability and transcription elongation impairment in human cells depleted of THO/TREX
    María S Domínguez-Sánchez
    Centro Andaluz de Biologia Molecular y Medicina Regenerativa CABIMER, Universidad de Sevilla Consejo Superior de Investigaciones Cientificas CSIC, Sevilla, Spain
    PLoS Genet 7:e1002386. 2011
    ....
  13. doi Causes of genome instability
    Andres Aguilera
    Centro Andaluz de Biologia Molecular y Medicina Regenerativa CABIMER, Universidad de Sevilla, 41092 Seville, Spain Email
    Annu Rev Genet 47:1-32. 2013
    ..Identifying the causes of genome instability is crucial to understanding genome dynamics during cell proliferation and its role in cancer, aging, and a number of rare genetic diseases. ..
  14. pmc Yeast Sen1 helicase protects the genome from transcription-associated instability
    Hannah E Mischo
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK
    Mol Cell 41:21-32. 2011
    ..Based on these findings, we propose that R loop formation is a frequent event during transcription and a key function of Sen1 is to prevent their accumulation and associated genome instability...
  15. doi Genome instability: a mechanistic view of its causes and consequences
    Andres Aguilera
    Centro Andaluz de Biologia Molecular y Medicina Regenerativa CABIMER, Universidad de Sevilla CSIC, Avd Américo Vespucio s n, 41092 Sevilla, Spain
    Nat Rev Genet 9:204-17. 2008
    ..Taking these elements as a guide, we review the causes and consequences of instability with the aim of providing a mechanistic perspective on the origin of genomic instability...
  16. pmc How to exchange your partner. Workshop on recombination mechanisms and the maintenance of genomic stability
    Andres Aguilera
    Centro Andaluz de Biologia Molecular y Medicina Regenerativa CABIMER, CSIC Universidad of Sevilla, 41092 Sevilla, Spain
    EMBO Rep 8:28-33. 2007
  17. pmc The connection between transcription and genomic instability
    Andres Aguilera
    Departamento de Genetica, Facultad de Biologia, Universidad de Sevilla, Sevilla 41012, Spain
    EMBO J 21:195-201. 2002
    ....
  18. ncbi Cotranscriptional mRNP assembly: from the DNA to the nuclear pore
    Andres Aguilera
    Departamento de Genetica, Facultad de Biologia, Universidad de Sevilla, Avd Reina Mercedes 6, 41012 Sevilla, Spain
    Curr Opin Cell Biol 17:242-50. 2005
    ..New findings suggest that mRNP assembly is a genetically controlled process that plays a key role in gene expression and other cellular processes, including the maintenance of genome integrity...
  19. doi A postincision-deficient TFIIH causes replication fork breakage and uncovers alternative Rad51- or Pol32-mediated restart mechanisms
    María Moriel-Carretero
    Centro Andaluz de Biologia Molecular y Medicina Regenerativa CABIMER, Universidad de Sevilla CSIC, Av Americo Vespucio s n, 41092 Sevilla, Spain
    Mol Cell 37:690-701. 2010
    ..Our results define the genetic and molecular hallmarks of replication fork breakage and restart and bring insights to understand specific NER-related human syndromes...
  20. pmc The THP1-SAC3-SUS1-CDC31 complex works in transcription elongation-mRNA export preventing RNA-mediated genome instability
    Cristina González-Aguilera
    Centro Andaluz de Biologia Molecular y Medicina Regenerativa, Universidad de Sevilla CSIC, 41092 Sevilla, Spain
    Mol Biol Cell 19:4310-8. 2008
    ....
  21. ncbi Different physiological relevance of yeast THO/TREX subunits in gene expression and genome integrity
    Maria Garcia-Rubio
    Departamento de Biologia Molecular, CABIMER, CSIC, Universidad de Sevilla, Av Americo Vespucio s n, Seville, Spain
    Mol Genet Genomics 279:123-32. 2008
    ..Our data provide new information on the relevance of these proteins in mRNP biogenesis and in the maintenance of genomic integrity...
  22. ncbi Equal sister chromatid exchange is a major mechanism of double-strand break repair in yeast
    Sergio González-Barrera
    Departamento de Genetica, Facultad de Biologia, Universidad de Sevilla, 41012 Seville, Spain
    Mol Cell 11:1661-71. 2003
    ..Our work provides a physical analysis of mitotically occurring SCE in vivo and opens new perspectives for the study and understanding of DSB repair in eukaryotes...
  23. pmc An hpr1 point mutation that impairs transcription and mRNP biogenesis without increasing recombination
    Pablo Huertas
    Departamento de Genetica, Facultad de Biologia, Universidad de Sevilla, Avd Reina Mercedes 6, 41012 Sevilla, Spain
    Mol Cell Biol 26:7451-65. 2006
    ....
  24. doi R loops are linked to histone H3 S10 phosphorylation and chromatin condensation
    Maikel Castellano-Pozo
    Department of Molecular Biology, Universidad de Sevilla CSIC, Avenida Américo Vespucio, 41092 Seville, Spain
    Mol Cell 52:583-90. 2013
    ..Such histone modifications were also observed in R-loop-accumulating Caenorhabditis elegans and HeLa cells. We therefore provide a role of RNA in chromatin structure essential to understand how R loops modulate genome dynamics. ..
  25. pmc Nab2 functions in the metabolism of RNA driven by polymerases II and III
    Cristina González-Aguilera
    Centro Andaluz de Biologia Molecular y Medicina Regenerativa, Universidad de Sevilla Consejo Superior de Investigaciones Científicas, 41092 Seville, Spain
    Mol Biol Cell 22:2729-40. 2011
    ....
  26. ncbi Molecular evidence that the eukaryotic THO/TREX complex is required for efficient transcription elongation
    Ana G Rondon
    Departamento de Genetica, Facultad de Biologia, Universidad de Sevilla, Avenida Reina Mercedes 6, 41012 Sevilla, Spain
    J Biol Chem 278:39037-43. 2003
    ..This work demonstrates that THO and Sub2 are required for efficient transcription elongation, providing further evidence for the coupling between transcription and mRNA metabolism and export...
  27. pmc The S-phase checkpoint is required to respond to R-loops accumulated in THO mutants
    Belén Gómez-González
    Centro Andaluz de Biologia Molecular y Medicina Regenerativa CABIMER, Universidad de Sevilla CSIC, Av Americo Vespucio s n, 41092 Seville, Spain
    Mol Cell Biol 29:5203-13. 2009
    ..In light of these results, we propose a model in which R-loop-mediated recombination is explained by template switching...
  28. pmc Tho1, a novel hnRNP, and Sub2 provide alternative pathways for mRNP biogenesis in yeast THO mutants
    Sonia Jimeno
    Departamento de Genetica, Facultad de Biologia, Universidad de Sevilla, Avd Reina Mercedes 6, 41012 Sevilla, Spain
    Mol Cell Biol 26:4387-98. 2006
    ..Overexpression of Tho1 or Sub2 may provide alternative ways for mRNP formation and export in the absence of a functional THO complex...
  29. pmc Defective nucleotide excision repair in yeast hpr1 and tho2 mutants
    Sergio González-Barrera
    Departamento de Genetica, Facultad de Biologia, Universidad de Sevilla, Avd Reina Mercedes 6, 41012 Sevilla, Spain
    Nucleic Acids Res 30:2193-201. 2002
    ..These results indicate that THO affects both transcription and NER. We discuss different alternatives to explain the effect of the THO complex on DNA repair...
  30. pmc Molecular evidence indicating that the yeast PAF complex is required for transcription elongation
    Ana G Rondon
    Departamento de Genetica, Facultad de Biologia, Universidad de Sevilla, Avd Reina Mercedes 6, 41012 Sevilla, Spain
    EMBO Rep 5:47-53. 2004
    ..Our work provides molecular evidence that PAF has a positive role in transcription elongation and is composed of at least two functionally different types of subunits (Paf1-Cdc73 and Leo1-Rtf1)...
  31. doi A reduction in RNA polymerase II initiation rate suppresses hyper-recombination and transcription-elongation impairment of THO mutants
    Sonia Jimeno
    Centro Andaluz de Biologia Molecular y Medicina Regenerativa CABIMER, Universidad de Sevilla CSIC, Av Americo Vespucio s n, 41092 Sevilla, Spain
    Mol Genet Genomics 280:327-36. 2008
    ..This study furthers the understanding of the importance of THO in transcription and the maintenance of genome stability...
  32. ncbi Interdependence between transcription and mRNP processing and export, and its impact on genetic stability
    Rosa Luna
    Departamento de Genetica, Facultad de Biologia, Universidad de Sevilla, Spain
    Mol Cell 18:711-22. 2005
    ..The results suggest a tight interdependence among mRNP biogenesis steps and transcription and an unexpected effect of the nuclear exosome and the cleavage/polyadenylation factors on transcription elongation and genetic integrity...
  33. pmc Molecular evidence for a positive role of Spt4 in transcription elongation
    Ana G Rondon
    Departamento de Genetica, Facultad de Biologia, Universidad de Sevilla, Avd Reina Mercedes 6, E 41012 Sevilla, Spain
    EMBO J 22:612-20. 2003
    ..Our results indicate that Spt4-Spt5 has a positive role in transcription elongation and suggest that Spt4-Spt5 and THO act at different steps during mRNA biogenesis...
  34. pmc Distinct roles of Mus81, Yen1, Slx1-Slx4, and Rad1 nucleases in the repair of replication-born double-strand breaks by sister chromatid exchange
    Sandra Muñoz-Galván
    Centro Andaluz de Biologia Molecular y Medicina Regenerativa, Universidad de Sevilla CSIC, Seville, Spain
    Mol Cell Biol 32:1592-603. 2012
    ..These unique effects, which have not been detected in other studies unless double mutant combinations were used, indicate the formation of distinct alternatives for the repair of replication-born DSBs that require specific SSEs...
  35. pmc Zim17/Tim15 links mitochondrial iron-sulfur cluster biosynthesis to nuclear genome stability
    María Del Carmen Díaz de la Loza
    Centro Andaluz de Biologia Molecular y Medicina Regenerativa CABIMER, Universidad de Sevilla CSIC, Avd Américo Vespucio, 41092 Sevilla, Spain
    Nucleic Acids Res 39:6002-15. 2011
    ..We propose that compromised ribosome biosynthesis and cell-cycle progression are interconnected, together contributing to replicative stress and nuclear genome instability in zim17Δ mutants...
  36. pmc R-loops do not accumulate in transcription-defective hpr1-101 mutants: implications for the functional role of THO/TREX
    Belén Gómez-González
    Centro Andaluz de Biologia Molecular y Medicina Regenerativa CABIMER, Universidad de Sevilla CSIC, 41092 Sevilla, Spain
    Nucleic Acids Res 37:4315-21. 2009
    ..These results indicate for the first time that THO has a transcriptional function that is not mediated by R-loops, providing a new perspective for the understanding of the coupling of transcription with mRNP biogenesis and export...
  37. pmc New suppressors of THO mutations identify Thp3 (Ypr045c)-Csn12 as a protein complex involved in transcription elongation
    Sonia Jimeno
    Centro Andaluz de Biologia Molecular y Medicina Regenerativa CABIMER, Seville, Spain
    Mol Cell Biol 31:674-85. 2011
    ..Thp3-Csn12 also copurifies with ribosomal proteins, which opens the possibility that it has other functions in addition to transcription...
  38. pmc Histone H3K56 acetylation, Rad52, and non-DNA repair factors control double-strand break repair choice with the sister chromatid
    Sandra Muñoz-Galván
    Centro Andaluz de Biologia Molecular y Medicina Regenerativa CABIMER, Universidad de Sevilla CSIC, Sevilla, Spain
    PLoS Genet 9:e1003237. 2013
    ..A dysfunction can lead to genome instability as manifested by high levels of homolog recombination and DSB accumulation...
  39. pmc The absence of the yeast chromatin assembly factor Asf1 increases genomic instability and sister chromatid exchange
    Felix Prado
    Departamento de Genetica, Facultad de Biologia, Universidad de Sevilla, Avda Reina Mercedes 6, 41012 Sevilla, Spain
    EMBO Rep 5:497-502. 2004
    ..Our results suggest that defective chromatin assembly caused by asf1Delta leads to DSBs that can be repaired by SCE, affecting genetic stability...
  40. pmc Transcription and double-strand breaks induce similar mitotic recombination events in Saccharomyces cerevisiae
    Sergio González-Barrera
    Departamento de Genetica, Facultad de Biologia, Universidad de Sevilla, Spain
    Genetics 162:603-14. 2002
    ..The similarity between transcription-associated and DSB-induced recombination suggests that transcription promotes DNA breaks...
  41. pmc Chromosomal translocations caused by either pol32-dependent or pol32-independent triparental break-induced replication
    Jose F Ruiz
    Centro Andaluz de Biologia Molecular y Medicina Regenerativa CABIMER, Universidad de Sevilla CSIC, Av Americo Vespucio s n, 41092 Sevilla, Spain
    Mol Cell Biol 29:5441-54. 2009
    ..Given the abundance of repetitive DNA in eukaryotic genomes, the results of this study open the possibility that HR rather than nonhomologous end joining may be a major source of chromosomal translocations...
  42. pmc A novel assay identifies transcript elongation roles for the Nup84 complex and RNA processing factors
    Cristina Tous
    Centro Andaluz de Biologia Molecular y Medicina Regenerativa CABIMER, Universidad de Sevilla CSIC, Sevilla, Spain
    EMBO J 30:1953-64. 2011
    ....
  43. pmc Partial depletion of histone H4 increases homologous recombination-mediated genetic instability
    Felix Prado
    Departamento de Genetica, Facultad de Biologia, Universidad de Sevilla, Avda Reina Mercedes 6, 41012 Seville, Spain
    Mol Cell Biol 25:1526-36. 2005
    ..Our results suggest that histone deposition defects lead to the formation of recombinogenic DNA structures during replication that increase genomic instability...
  44. ncbi Nab2p and the Thp1p-Sac3p complex functionally interact at the interface between transcription and mRNA metabolism
    Mercedes Gallardo
    Departamento de Genetica, Facultad de Biologia, Universidad de Sevilla, Avenida Reina Mercedes 6, 41012 Sevilla, Spain
    J Biol Chem 278:24225-32. 2003
    ..Altogether, our results suggest that Thp1p-Sac3p and Nab2p are functionally related heterogeneous nuclear ribonucleoproteins that define a further link between mRNA metabolism and transcription...
  45. pmc R-loops cause replication impairment and genome instability during meiosis
    Maikel Castellano-Pozo
    Centro Andaluz de Biologia Molecular y Medicina Regenerativa CABIMER, Universidad de Sevilla CSIC, Sevilla, Spain
    EMBO Rep 13:923-9. 2012
    ..Importantly, RNase H partially suppressed the replication impairment and the DNA-damage accumulation. We conclude that R-loops can form during meiosis causing replication impairment with deleterious results...
  46. pmc Genome-wide function of THO/TREX in active genes prevents R-loop-dependent replication obstacles
    Belén Gómez-González
    Centro Andaluz de Biologia Molecular y Medicina Regenerativa CABIMER, Universidad de Sevilla CSIC, Sevilla, Spain
    EMBO J 30:3106-19. 2011
    ..Our work establishes a genome-wide function for THO-Sub2 in transcription elongation and mRNP biogenesis that function to prevent the accumulation of transcription-mediated replication obstacles, including R-loops...
  47. ncbi Cotranscriptionally formed DNA:RNA hybrids mediate transcription elongation impairment and transcription-associated recombination
    Pablo Huertas
    Departamento de Genetica, Facultad de Biologia, Universidad de Sevilla, Avenida Reina, Mercedes 6, 41012 Seville, Spain
    Mol Cell 12:711-21. 2003
    ..These data support a model to explain the connection between recombination, transcription, and mRNA metabolism and provide a new perspective to understanding transcription-associated recombination...
  48. pmc Impairment of replication fork progression mediates RNA polII transcription-associated recombination
    Felix Prado
    Departamento de Genetica, Facultad de Biologia, Universidad de Sevilla, Sevilla, Spain
    EMBO J 24:1267-76. 2005
    ..Therefore, our work provides evidence that one mechanism responsible for TAR is RNAP-mediated replication impairment...
  49. doi Biogenesis of mRNPs: integrating different processes in the eukaryotic nucleus
    Rosa Luna
    Centro Andaluz de Biologia Molecular y Medicina Regenerativa CABIMER, Universidad de Sevilla CSIC, Seville, Spain
    Chromosoma 117:319-31. 2008
    ..Here, we review the overall process of mRNP biogenesis with particular emphasis on the functional coupling of transcription with mRNP biogenesis and export and its relationship to nuclear organization...
  50. pmc The Npl3 hnRNP prevents R-loop-mediated transcription-replication conflicts and genome instability
    José M Santos-Pereira
    Centro Andaluz de Biologia Molecular y Medicina Regenerativa CABIMER, Universidad de Sevilla Consejo Superior de Investigaciones Cientificas CSIC, 41092 Seville, Spain
    Genes Dev 27:2445-58. 2013
    ..Therefore, our work demonstrates that mRNP factors are critical for genome integrity and opens the option of using them as therapeutic targets in anti-cancer treatment. ..
  51. doi New clues to understand the role of THO and other functionally related factors in mRNP biogenesis
    Rosa Luna
    Centro Andaluz de Biologia Molecular y Medicina Regenerativa CABIMER, Universidad de Sevilla CSIC, Av Americo Vespucio s n, 41092 Sevilla, Spain
    Biochim Biophys Acta 1819:514-20. 2012
    ..This article is part of a Special Issue entitled: Nuclear Transport and RNA Processing...
  52. pmc The yeast and human FACT chromatin-reorganizing complexes solve R-loop-mediated transcription-replication conflicts
    Emilia Herrera-Moyano
    Centro Andaluz de Biologia Molecular y Medicina Regenerativa CABIMER, Universidad de Sevilla, 41092 Seville, Spain
    Genes Dev 28:735-48. 2014
    ..The results demonstrate a key function of FACT in the resolution of R-loop-mediated transcription-replication conflicts, likely associated with a specific chromatin organization. ..
  53. pmc Activation-induced cytidine deaminase action is strongly stimulated by mutations of the THO complex
    Belén Gómez-González
    Departamento de Genetica, Facultad de Biologia, Consejo Superior de Investigaciones Científicas Universidad de Sevilla, Avenida Americo Vespucio s n, 41092 Sevilla, Spain
    Proc Natl Acad Sci U S A 104:8409-14. 2007
    ..We propose that a suboptimal cotranscriptional mRNP assembly at particular DNA regions could play an important role in Ig diversification and genome dynamics...
  54. pmc A novel yeast mutation, rad52-L89F, causes a specific defect in Rad51-independent recombination that correlates with a reduced ability of Rad52-L89F to interact with Rad59
    Felipe Cortes-Ledesma
    Departamento de Genetica, Facultad de Biologia, Universidad de Sevilla, 41012, Spain
    Genetics 168:553-7. 2004
    ..These results support the idea that Rad59 acts in homologous recombination via physical interaction with Rad52...
  55. pmc Control of the function of the transcription and repair factor TFIIH by the action of the cochaperone Ydj1
    María Moriel-Carretero
    Centro Andaluz de Biologia Molecular y Medicina Regenerativa, Universidad de Sevilla Consejo Superior de Investigaciones Científicas, 41092 Seville, Spain
    Proc Natl Acad Sci U S A 108:15300-5. 2011
    ..Our results provide evidence for a role of chaperones in NER and transcription, with implications in cancer and TFIIH-associated syndromes...
  56. pmc The Caenorhabditis elegans THO complex is required for the mitotic cell cycle and development
    Maikel Castellano-Pozo
    Department of Molecular Biology, Centro Andaluz de Biologia Molecular y Medicina Regenerativa, Universidad de Sevilla, Sevilla, Spain
    PLoS ONE 7:e52447. 2012
    ..Using Caenorhabditis elegans as a model system we show that the THO complex is essential for mitotic genome integrity and the developmentally regulated mitotic cell cycles occurring during late postembryonic stages...
  57. doi The interface between transcription and mRNP export: from THO to THSC/TREX-2
    Ana G Rondon
    Centro Andaluz de Biologia Molecular y Medicina Regenerativa CABIMER, Universidad de Sevilla CSIC, Av Americo Vespucio s n, 41092 Sevilla, Spain
    Biochim Biophys Acta 1799:533-8. 2010
    ..Here we review our actual knowledge of the role that these factors play at the interface between transcription and mRNA export in the model organism Saccharomyces cerevisiae...
  58. pmc Differential expression of THOC1 and ALY mRNP biogenesis/export factors in human cancers
    María S Domínguez-Sánchez
    Centro Andaluz de Biologia Molecular y Medicina Regenerativa CABIMER, Universidad de Sevilla CSIC, Av, Americo Vespucio s n, 41092 Sevilla, Spain
    BMC Cancer 11:77. 2011
    ..Given the link between genome instability and cancer, we have performed a comparative analysis of the expression patterns of THOC1, a THO complex subunit, and ALY in tumor samples...
  59. pmc Replication fork progression is impaired by transcription in hyperrecombinant yeast cells lacking a functional THO complex
    Ralf E Wellinger
    Departamento de Genetica, Facultad de Biologia, Universidad de Sevilla, Avda Reina Mercedes 6, 41012 Seville, Spain
    Mol Cell Biol 26:3327-34. 2006
    ..Our results link transcription-dependent genomic instability in THO mutants with impaired replication fork progression, suggesting a molecular basis for a connection between inefficient mRNP biogenesis and genetic instability...
  60. pmc Double-strand breaks arising by replication through a nick are repaired by cohesin-dependent sister-chromatid exchange
    Felipe Cortes-Ledesma
    Departamento de Genetica, Facultad de Biologia, Universidad de Sevilla, Avd Reina Mercedes 6, 41012 Sevilla, Spain
    EMBO Rep 7:919-26. 2006
    ..This work opens new perspectives to understand the importance of single-strand breaks as a source of recombination and the relevance of cohesion in the repair of replication-born DSBs...
  61. ncbi Relationship between G+C content, ORF-length and mRNA concentration in Saccharomyces cerevisiae
    Antonio Marin
    Departamento de Genetica, Facultad de Biologia, Universidad de Sevilla, Avd Reina Mercedes 6, 41012 Sevilla, Spain
    Yeast 20:703-11. 2003
    ..We discuss the biological relevance of these results, as well as different ways that these structural features could modulate mRNA biogenesis...
  62. doi Methods to study transcription-coupled repair in chromatin
    Hélène Gaillard
    CABIMER, Universidad de Sevilla CSIC, Sevilla, Spain
    Methods Mol Biol 523:141-59. 2009
    ..Here, we provide a detailed protocol for both the DNA repair analysis and the ChIP approaches to study TCR in chromatin...
  63. ncbi SMC proteins, new players in the maintenance of genomic stability
    Felipe Cortes-Ledesma
    Department of Molecular Biology, CABIMER, CSIC Universidad de Sevilla, Sevilla, Spain
    Cell Cycle 6:914-8. 2007
    ..A deeper knowledge of the role of SMC proteins in DSB repair should contribute to a better understanding of chromosome dynamics and stability...
  64. ncbi Mitotic recombination in Saccharomyces cerevisiae
    Felix Prado
    Departamento de Genetica, Facultad de Biologia, Universidad de Sevilla, 41012 Seville, Spain
    Curr Genet 42:185-98. 2003
    ..Here, we review recent genetic yeast data that contribute to our understanding of the different mechanisms of mitotic recombination and the in vivo role of the recombination proteins...
  65. pmc A novel class of mRNA-containing cytoplasmic granules are produced in response to UV-irradiation
    Hélène Gaillard
    Departamento de Genetica, Facultad de Biologia, Universidad de Sevilla, Centro Andaluz de Biología Molecular and Medicina Regenativa CABIMER, 41092 Sevilla, Spain
    Mol Biol Cell 19:4980-92. 2008
    ..We propose that potentially damaged mRNAs, which may be deleterious to the cell, are temporarily stored in UVG granules to safeguard cell viability...
  66. ncbi Transcription at the proximity of the nuclear pore: a role for the THP1-SAC3-SUS1-CDC31 (THSC) complex
    Rosa Luna
    Centro Andaluz de Biologia Molecular y Medicina Regenerativa CABIMER, Universidad de Sevilla CSIC, Seville, Spain
    RNA Biol 6:145-8. 2009
    ..Here we discuss the new data on the coupling of gene expression and RNA export, with emphasis on the THP1-SAC3-SUS1-CDC31 complex and the possible implications of these results on transcription at the nuclear pore...
  67. doi Transcription coupled repair at the interface between transcription elongation and mRNP biogenesis
    Hélène Gaillard
    Universidad de Sevilla, Sevilla, Spain
    Biochim Biophys Acta 1829:141-50. 2013
    ..This article is part of a Special Issue entitled: RNA polymerase II Transcript Elongation...
  68. ncbi Impairment of transcription elongation by R-loops in vitro
    Cristina Tous
    Departamento de Genetica, Facultad de Biologia, and Departamento de Biología Molecular, CABIMER, CSIC Universidad de Sevilla, Av Americo Vespucio s n, 41092 Sevilla, Spain
    Biochem Biophys Res Commun 360:428-32. 2007
    ....
  69. doi Genetic and molecular analysis of mitotic recombination in Saccharomyces cerevisiae
    Belén Gómez-González
    Centro Andaluz de Biologia Molecular y Medicina Regenerativa, Universidad de Sevilla CSIC, Sevilla, Spain
    Methods Mol Biol 745:151-72. 2011
    ....
  70. ncbi Control of cross-over by single-strand DNA resection
    Felix Prado
    Departamento de Genetica, Facultad de Biologi a, Universidad de Sevilla, 41012, Sevilla, Spain
    Trends Genet 19:428-31. 2003
    ..Extensive DNA resection could be an effective mechanism to prevent reciprocal exchanges between dispersed DNA sequences, and thus contribute to the genome stability...
  71. pmc Yeast pol4 promotes tel1-regulated chromosomal translocations
    Jose F Ruiz
    Centro de Biologia Molecular Severo Ochoa, Universidad Autonoma de Madrid CSIC, Madrid, Spain
    PLoS Genet 9:e1003656. 2013
    ..Overall, this work provides further insight to the molecular mechanisms of DSB repair by NHEJ and presents a new perspective to the understanding of how chromosomal translocations are formed in eukaryotic cells. ..