Juan C Stockert

Summary

Country: Spain

Publications

  1. ncbi request reprint DNA-binding fluorochromes: correlation between C-banding of mouse metaphase chromosomes and hydrogen bonding to adenine-thymine base pairs
    Juan C Stockert
    Department of Biology, Faculty of Sciences, Autonomous University of Madrid, Cantoblanco, 28049 Madrid, Spain
    Acta Histochem 106:413-20. 2005
  2. ncbi request reprint Morphological criteria to distinguish cell death induced by apoptotic and necrotic treatments
    S Rello
    Departamento de Biologia, Facultad de Ciencias, Universidad Autonoma de Madrid, Ciudad Universitaria de Cantoblanco, E 28049, Madrid, Spain
    Apoptosis 10:201-8. 2005
  3. doi request reprint MTT assay for cell viability: Intracellular localization of the formazan product is in lipid droplets
    Juan C Stockert
    Department of Biology, Faculty of Sciences, Autonomous University of Madrid, Spain
    Acta Histochem 114:785-96. 2012
  4. ncbi request reprint Porphycenes: facts and prospects in photodynamic therapy of cancer
    J C Stockert
    Departamento de Biologia, Facultad de Ciencias, Universidad Autonoma de Madrid, 28049 Madrid, Spain
    Curr Med Chem 14:997-1026. 2007
  5. doi request reprint Selective labeling of lipid droplets in aldehyde fixed cell monolayers by lipophilic fluorochromes
    J C Stockert
    Department of Biology, Faculty of Sciences, Autonomous University of Madrid, Madrid 28049, Spain
    Biotech Histochem 85:277-83. 2010
  6. ncbi request reprint Cytochemistry of mast cells: new fluorescent methods selective for sulfated glycosaminoglycans
    J C Stockert
    Departamento de Biologia, Facultad de Ciencias, Universidad Autonoma de Madrid, Canto Blanco, Spain
    Acta Histochem 102:259-72. 2000
  7. doi request reprint Induction of metachromasia in cationic dyes and fluorochromes using a clay mineral: a potentially valuable model for histochemical studies
    Juan C Stockert
    Department of Biology, Faculty of Sciences, Autonomous University of Madrid, Cantoblanco, Spain
    Acta Histochem 113:668-70. 2011
  8. doi request reprint Inaccurate chemical structures of dyes and fluorochromes found in the literature can be problematic for teaching and research
    J C Stockert
    Department of Biology, Faculty of Sciences, Autonomous University of Madrid, Madrid 28049, Spain
    Biotech Histochem 86:52-60. 2011
  9. doi request reprint Improving images of fluorescent cell labeling by background signal subtraction
    J C Stockert
    Department of Biology, Faculty of Sciences, Autonomous University of Madrid, Cantoblanco, 28049 Madrid, Spain
    Biotech Histochem 84:63-8. 2009
  10. doi request reprint A mechanism for the fluorogenic reaction of amino groups with fluorescamine and MDPF
    Juan C Stockert
    Department of Biology, Faculty of Sciences, Autonomous University of Madrid, Cantoblanco, 28049 Madrid, Spain
    Acta Histochem 110:333-40. 2008

Collaborators

Detail Information

Publications37

  1. ncbi request reprint DNA-binding fluorochromes: correlation between C-banding of mouse metaphase chromosomes and hydrogen bonding to adenine-thymine base pairs
    Juan C Stockert
    Department of Biology, Faculty of Sciences, Autonomous University of Madrid, Cantoblanco, 28049 Madrid, Spain
    Acta Histochem 106:413-20. 2005
    ....
  2. ncbi request reprint Morphological criteria to distinguish cell death induced by apoptotic and necrotic treatments
    S Rello
    Departamento de Biologia, Facultad de Ciencias, Universidad Autonoma de Madrid, Ciudad Universitaria de Cantoblanco, E 28049, Madrid, Spain
    Apoptosis 10:201-8. 2005
    ....
  3. doi request reprint MTT assay for cell viability: Intracellular localization of the formazan product is in lipid droplets
    Juan C Stockert
    Department of Biology, Faculty of Sciences, Autonomous University of Madrid, Spain
    Acta Histochem 114:785-96. 2012
    ..These predictions were in agreement with experimental observations on the accumulation of MTT formazan product in lipid droplets...
  4. ncbi request reprint Porphycenes: facts and prospects in photodynamic therapy of cancer
    J C Stockert
    Departamento de Biologia, Facultad de Ciencias, Universidad Autonoma de Madrid, 28049 Madrid, Spain
    Curr Med Chem 14:997-1026. 2007
    ..Taking into account all these features, porphycene PSs should be very useful for PDT of cancer and other biomedical applications...
  5. doi request reprint Selective labeling of lipid droplets in aldehyde fixed cell monolayers by lipophilic fluorochromes
    J C Stockert
    Department of Biology, Faculty of Sciences, Autonomous University of Madrid, Madrid 28049, Spain
    Biotech Histochem 85:277-83. 2010
    ....
  6. ncbi request reprint Cytochemistry of mast cells: new fluorescent methods selective for sulfated glycosaminoglycans
    J C Stockert
    Departamento de Biologia, Facultad de Ciencias, Universidad Autonoma de Madrid, Canto Blanco, Spain
    Acta Histochem 102:259-72. 2000
    ....
  7. doi request reprint Induction of metachromasia in cationic dyes and fluorochromes using a clay mineral: a potentially valuable model for histochemical studies
    Juan C Stockert
    Department of Biology, Faculty of Sciences, Autonomous University of Madrid, Cantoblanco, Spain
    Acta Histochem 113:668-70. 2011
    ..These spectral results indicate that on account of its strong aggregating capacity, montmorillonite is a suitable model substrate to study metachromatic reactions...
  8. doi request reprint Inaccurate chemical structures of dyes and fluorochromes found in the literature can be problematic for teaching and research
    J C Stockert
    Department of Biology, Faculty of Sciences, Autonomous University of Madrid, Madrid 28049, Spain
    Biotech Histochem 86:52-60. 2011
    ....
  9. doi request reprint Improving images of fluorescent cell labeling by background signal subtraction
    J C Stockert
    Department of Biology, Faculty of Sciences, Autonomous University of Madrid, Cantoblanco, 28049 Madrid, Spain
    Biotech Histochem 84:63-8. 2009
    ....
  10. doi request reprint A mechanism for the fluorogenic reaction of amino groups with fluorescamine and MDPF
    Juan C Stockert
    Department of Biology, Faculty of Sciences, Autonomous University of Madrid, Cantoblanco, 28049 Madrid, Spain
    Acta Histochem 110:333-40. 2008
    ..Although the pseudobases of fluorescamine- and MDPF-amino derivatives are formed at alkaline pH, a full conjugated, coplanar and cationic molecule is suggested to be the true fluorescent product...
  11. doi request reprint Vehiculization determines the endocytic internalization mechanism of Zn(II)-phthalocyanine
    Jorge Soriano
    Departamento de Biologia, Facultad de Ciencias, Universidad Autonoma de Madrid, 28049 Madrid, Spain
    Histochem Cell Biol 139:149-60. 2013
    ..We conclude that using different drug vehiculization systems, it is possible to modify the internalization mechanism of a therapeutic compound, which could be of great interest in clinical research...
  12. ncbi request reprint Caspase-2: a possible trigger of apoptosis induced in A-549 tumor cells by ZnPc photodynamic treatment
    Javier Cristóbal
    Departamento de Biologia, Facultad de Ciencias, Universidad Autonoma de Madrid, Cantoblanco, Madrid, Spain
    Int J Oncol 28:1057-63. 2006
    ..From this results we assume that following the photodynamic treatment of A-549 cells with ZnPc, the activation of caspase-2 in the Golgi apparatus could begin to initiate immediately the apoptotic process...
  13. doi request reprint Cell uptake of Zn(II)-phthalocyanine-containing liposomes by clathrin-mediated endocytosis
    Jorge Soriano
    Departamento de Biologia, Facultad de Ciencias, Universidad Autonoma de Madrid, Darwin 2, 28049, Madrid, Spain
    Histochem Cell Biol 133:449-54. 2010
    ..These results indicate that ZnPc is internalized into cells preferentially by a mechanism of clathrin-mediated endocytosis...
  14. doi request reprint Differential photodynamic response of cultured cells to methylene blue and toluidine blue: role of dark redox processes
    Alfonso Blazquez-Castro
    Department of Biology, Faculty of Sciences, Autonomous University of Madrid, 28049, Madrid, Spain
    Photochem Photobiol Sci 8:371-6. 2009
    ..We propose that massive reduction of MB to its leuco form inside the cell inhibits most of the photodynamic damage, while no such reduction occurs with TB...
  15. doi request reprint Tumour cell death induced by the bulk photovoltaic effect of LiNbO3:Fe under visible light irradiation
    Alfonso Blazquez-Castro
    Departamento de Biologia, Facultad de Ciencias, Universidad Autonoma de Madrid, Madrid, Spain
    Photochem Photobiol Sci 10:956-63. 2011
    ..Necrosis also occurred when cultured tumour cells were exposed to LiNbO(3):Fe microparticles and visible light...
  16. ncbi request reprint Photokilling of cultured tumour cells by the porphyrin derivative CF3
    A Villanueva
    Departamento de Biologia, Facultad de Ciencias, Universidad Autonoma de Madrid, Spain
    Anticancer Drug Des 16:279-90. 2001
    ..1%). Forty-eight hours after this metaphase blockage, cultures showed a great number of apoptotic cells. Taking into account these results, CF3 could be a valuable photosensitizer for the photodynamic therapy of cancer...
  17. ncbi request reprint Photokilling mechanisms induced by zinc(II)-phthalocyanine on cultured tumor cells
    A Villanueva
    Departamento de Biologia, Facultad de Ciencias, Universidad Autonoma de Madrid, Canto Blanco, Spain
    Oncol Res 11:447-53. 1999
    ..Both apoptotic and necrotic mechanisms for cell death occur in HeLa cells in dependence on the experimental protocol of ZnPc photodynamic treatments...
  18. ncbi request reprint Necrotic cell death induced by photodynamic treatment of human lung adenocarcinoma A-549 cells with palladium(II)-tetraphenylporphycene
    M Canete
    Departamento de Biologia, Facultad de Ciencias, Universidad Autonoma de Madrid, Cantoblanco, 28049 Madrid, Spain
    Int J Oncol 24:1221-8. 2004
    ..Present results show that the cell type plays a fundamental role in relation to the apoptotic or necrotic response to photosensitization, and that cytoskeletal components are important targets implicated in cell death processes...
  19. ncbi request reprint Photodamage induced by Zinc(II)-phthalocyanine to microtubules, actin, alpha-actinin and keratin of HeLa cells
    A Juarranz
    Departamento de Biologia, Facultad de Ciencias, Universidad Autonoma de Madrid, Canto Blanco, E 28049 Madrid, Spain
    Photochem Photobiol 73:283-9. 2001
    ....
  20. ncbi request reprint Fluorogenic reaction of blood cells induced by N-bromosuccinimide
    Angel Martinez-Ramirez
    Department of Biology, Faculty of Sciences, Autonomous University of Madrid, Canto Blanco, E 28049 Madrid, Spain
    Micron 33:399-402. 2002
    ..Possible mechanisms underlying these results are discussed and we propose NBS as a very suitable fluorogenic reagent for the microscopical detection and analysis of proteins...
  21. doi request reprint Binding of cationic dyes to DNA: distinguishing intercalation and groove binding mechanisms using simple experimental and numerical models
    P Del Castillo
    Department of Biology, Faculty of Sciences, Autonomous University of Madrid, Madrid, Spain
    Biotech Histochem 85:247-56. 2010
    ..We demonstrated that low aspect ratio dyes bound by intercalation, whereas more rod-like dyes were groove binders. Some problems that result and possible applications are discussed briefly...
  22. ncbi request reprint Fluorescence reaction of chromatin by curcumin
    J C Stockert
    , Facultad de Ciencias, , Spain
    Z Naturforsch C 44:327-9. 1989
    ..The selective fluorescence characteristics of curcumin support the possibility of employing this dye as a new fluorochrome...
  23. ncbi request reprint Fixation and permanent mounting of fluorescent probes after vital labelling of cultured cells
    M Canete
    Departamento de Biologia, Facultad de Ciencias, Universidad Autonoma de Madrid, Canto Blanco, Spain
    Acta Histochem 103:117-26. 2001
    ..The method of vapour fixation and DPX mounting is simple, rapid and reproducible, allowing definitive preservation of the fluorescence pattern observed in unfixed cell cultures...
  24. ncbi request reprint Selective electronmicroscopical contrast of the ring canals joining germ cells by ethanolic phosphotungstic acid and uranyl-EDTA-lead
    J C Stockert
    Departamento de Biologia, Facultad de Ciencias, Universidad Autonoma de Madrid, Spain
    Acta Histochem 82:171-6. 1987
    ..The contrasting reaction of this fibrous structure, which originates from the contractile ring during previous cytokinesis, is briefly discussed...
  25. ncbi request reprint Recycling cultured cells for immunofluorescent labeling
    J Espada
    Departamento de Bioquimica, Instituto de Investigaciones Biomedicas, CSIC, Facultad de Medicina, Universidad Autonoma de Madrid, Arturo Duperier 4, 28029 Madrid, Spain
    Histochem Cell Biol 116:41-7. 2001
    ..In addition, the method is also useful to recycle blotted membranes in immunoblots...
  26. ncbi request reprint Tungsten and molybdenum heteropolyacids as staining and contrasting agents: reactivity with epoxyresin-embedded cell and tissue structures
    J C Stockert
    Departamento de Biologia, Facultad de Ciencias, Universidad Autonoma de Madrid, Spain
    Acta Histochem 86:151-8. 1989
    ....
  27. ncbi request reprint Fluorescence of chromatin DNA induced by antitumoral naphthalimides
    J C Stockert
    , Facultad de Ciencias, , Spain
    Z Naturforsch C 52:408-11. 1997
    ..Fluorescence properties open new and interesting applications for some of these antitumoral and DNA-intercalating naphthalimides...
  28. ncbi request reprint Fluorescent cytochemistry of acid phosphatase and demonstration of fluid-phase endocytosis using an azo dye method
    J Espada
    Instituto de Investigaciones Biomedicas, CSIC, Departamento de Bioquimica, Universidad Autonoma de Madrid, Spain
    Histochem Cell Biol 108:481-7. 1997
    ..The differing uptake mechanisms, and the occurrence of lysosomally localized azo dye, were also in keeping with the predictions of quantitative structure-activity relationship models of this system...
  29. ncbi request reprint DNA fluorescence induced by polymethine cation pyrvinium binding
    J C Stockert
    Departamento de Biologia, Facultad de Ciencias, Universidad Autonoma de Madrid, Spain
    Histochem J 23:548-52. 1991
    ..Experimental results and the chemical structure of pyrvinium allow us to suggest that the minor groove of adenine-thymine DNA regions could be the specific binding site for this new DNA fluorochrome...
  30. ncbi request reprint Long-term regression of the murine mammary adenocarcinoma, LM3, by repeated photodynamic treatments using meso-tetra (4-N-methylpyridinium) porphine
    Lucas L Colombo
    Area Investigacion, Instituto de Oncología A H Roffo, Universidad de Buenos Aires, Buenos Aires, Argentina
    Int J Oncol 27:1053-9. 2005
    ..These results indicate the considerable potential of intratumoral injection of photosensitizers and repeated PDT protocols...
  31. ncbi request reprint Photochemical production and characterisation of the radical ions of tetraphenylporphycenes
    Noemi Rubio
    Grup d Enginyeria Molecular, Institut Quimic de Sarria, Universitat Ramon Llull, Via Augusta 390, 08017, Barcelona, Spain
    Photochem Photobiol Sci 5:376-80. 2006
    ..The results provide deeper insight on the role of these radical ion species in the photodynamic activity of tetraphenylporphycenes...
  32. ncbi request reprint Photodynamic toxicity and its prevention by antioxidative agents in Bufo arenarum embryos
    Juan C Stockert
    Instituto de Ciencias Ambientales y Salud, Fundacion PROSAMA, Paysandú 752 760, 1405 Buenos Aires, Argentina
    Toxicology 192:211-8. 2003
    ..2 J/cm2 irradiation. These results indicate that both the lethal photodynamic effect and its prevention by antioxidants can be evaluated by means of a simple toxicity test employing amphibian embryos...
  33. ncbi request reprint Human DNA methyltransferase 1 is required for maintenance of the histone H3 modification pattern
    Jesus Espada
    Epigenetics Laboratory, Spanish National Cancer Centre CNIO, Melchor Fernandez Almagro 3, 28029 Madrid, Spain
    J Biol Chem 279:37175-84. 2004
    ..Our data indicate that DNMT1 activity, via maintenance of the appropriate histone H3 modifications, contributes to the preservation of the correct organization of large heterochromatic regions...
  34. ncbi request reprint Loss of E-cadherin mediated cell-cell adhesion as an early trigger of apoptosis induced by photodynamic treatment
    Sergio Galaz
    Department of Biology and Health, Faculty of Sciences, University of Tarapaca, Arica, Chile
    J Cell Physiol 205:86-96. 2005
    ..These results strongly suggest that the E-cadherin adhesion complex could be the primary target of ZnPc phototreatment, and that loss of E-cadherin mediated cell adhesion after early photodamage triggers an apoptotic response...
  35. ncbi request reprint Fluorescent cationic probes for nuclei of living cells: why are they selective? A quantitative structure-activity relations analysis
    Richard W Horobin
    Division of Neuroscience and Biomedical Systems, Institute of Biomedical and Life Sciences, The University of Glasgow, West Medical Building, G12 8QQ Glasgow, Scotland, UK
    Histochem Cell Biol 126:165-75. 2006
    ..Preliminary applications of the QSAR model--to the selection of anticancer drugs, minimization of dye and drug toxicity and the designed synthesis of fluorescent probes-are outlined...
  36. doi request reprint Disorganisation of cytoskeleton in cells resistant to photodynamic treatment with decreased metastatic phenotype
    Adriana Casas
    Centro de Investigaciones sobre Porfirinas y Porfirias, CONICET and Hospital de Clínicas José de San Martín, Universidad de Buenos Aires, Cordoba 2351, 1er Subsuelo, Ciudad de Buenos Aires, Argentina
    Cancer Lett 270:56-65. 2008
    ..Changes in the metastasis phenotypes previously found are likely to be ascribed to these differences...
  37. ncbi request reprint Non-aqueous permanent mounting for immunofluorescence microscopy
    Jesus Espada
    Molecular Pathology Program, Spanish National Cancer Center CNIO, Institute of Health Carlos III, 28029 Madrid, Spain
    Histochem Cell Biol 123:329-34. 2005
    ..Using this non-aqueous mounting medium, the fluorescent signal remains high and stable, allowing a suitable and permanent preservation of labelled and counterstained microscopical preparations...