Juan Luis Ramos

Summary

Country: Spain

Publications

  1. pmc Efflux pumps involved in toluene tolerance in Pseudomonas putida DOT-T1E
    J L Ramos
    Department of Biochemistry and Molecular and Cellular Biology of Plants, Consejo Superior de Investigaciones Cientificas, Granada, Spain
    J Bacteriol 180:3323-9. 1998
  2. pmc Lessons from the genome of a lithoautotroph: making biomass from almost nothing
    Juan Luis Ramos
    Department of Biochemistry and Molecular and Cellular Biology of Plants, Estacion Experimental del Zaidin, Consejo Superior de Investigaciones Cientificas, 18008 Granada, Spain
    J Bacteriol 185:2690-1. 2003
  3. ncbi request reprint Compensatory role of the cis-trans-isomerase and cardiolipin synthase in the membrane fluidity of Pseudomonas putida DOT-T1E
    Patricia Bernal
    Consejo Superior de Investigaciones Científicas Estación Experimental del Zaidín, Department of Environmental Protection, Profesor Albareda 1, E 18008 Granada, Spain
    Environ Microbiol 9:1658-64. 2007
  4. pmc Rhizoremediation of lindane by root-colonizing Sphingomonas
    Dietmar Böltner
    Consejo Superior de Investigaciones Cientificas, Eslación Experimental del Zaidin, Department of Environmental Protection, C Prof Albareda, 1, E 18008 Granada, Spain
    Microb Biotechnol 1:87-93. 2008
  5. pmc Microbial responses to xenobiotic compounds. Identification of genes that allow Pseudomonas putida KT2440 to cope with 2,4,6-trinitrotoluene
    Matilde Fernandez
    Bio Iliberis Research and Development, Edificio BIC, Parque Tecnológico de Ciencias de la Salud, E 18100 Armilla, Granada, Spain
    Microb Biotechnol 2:287-94. 2009
  6. pmc Physiological responses of Pseudomonas putida to formaldehyde during detoxification
    Amalia Roca
    Department of Environmental Protection, Consejo Superior de Investigaciones Cientificas, C Prof Albareda, 1, E 18008 Granada, Spain
    Microb Biotechnol 1:158-69. 2008
  7. pmc Characterization of the RND family of multidrug efflux pumps: in silico to in vivo confirmation of four functionally distinct subgroups
    Patricia Godoy
    Consejo Superior de Investigaciones Cientificas, Department of Environmental Protection, E 18008 Granada, Spain
    Microb Biotechnol 3:691-700. 2010
  8. doi request reprint Laboratory research aimed at closing the gaps in microbial bioremediation
    Juan Luis Ramos
    CSIC, Estacion Experimental del Zaidin, Granada, Spain
    Trends Biotechnol 29:641-7. 2011
  9. pmc Genomic analysis reveals the major driving forces of bacterial life in the rhizosphere
    Miguel A Matilla
    Department of Environmental Protection, Estación Experimental de Zaidín, Consejo Superior de Investigaciones Cientificas, Profesor Albareda 1, Granada 18008, Spain
    Genome Biol 8:R179. 2007
  10. doi request reprint Responses of Pseudomonas to small toxic molecules by a mosaic of domains
    Juan L Ramos
    Consejo Superior de Investigaciones Científica, Estacion Experimental del Zaidin, Department of Environmental Protection, Granada, Spain
    Curr Opin Microbiol 12:215-20. 2009

Collaborators

Detail Information

Publications81

  1. pmc Efflux pumps involved in toluene tolerance in Pseudomonas putida DOT-T1E
    J L Ramos
    Department of Biochemistry and Molecular and Cellular Biology of Plants, Consejo Superior de Investigaciones Cientificas, Granada, Spain
    J Bacteriol 180:3323-9. 1998
    ..The mutation in P. putida DOT-T1E-18 was cloned, and the knockedout gene was sequenced and found to be homologous to the drug exclusion gene mexB, which belongs to the efflux pump family of the resistant nodulator division type...
  2. pmc Lessons from the genome of a lithoautotroph: making biomass from almost nothing
    Juan Luis Ramos
    Department of Biochemistry and Molecular and Cellular Biology of Plants, Estacion Experimental del Zaidin, Consejo Superior de Investigaciones Cientificas, 18008 Granada, Spain
    J Bacteriol 185:2690-1. 2003
  3. ncbi request reprint Compensatory role of the cis-trans-isomerase and cardiolipin synthase in the membrane fluidity of Pseudomonas putida DOT-T1E
    Patricia Bernal
    Consejo Superior de Investigaciones Científicas Estación Experimental del Zaidín, Department of Environmental Protection, Profesor Albareda 1, E 18008 Granada, Spain
    Environ Microbiol 9:1658-64. 2007
    ..However, the cls and cls/cti mutants were more sensitive to solvents than the wild-type and the cti mutant because of the impaired functioning of efflux drug transporters...
  4. pmc Rhizoremediation of lindane by root-colonizing Sphingomonas
    Dietmar Böltner
    Consejo Superior de Investigaciones Cientificas, Eslación Experimental del Zaidin, Department of Environmental Protection, C Prof Albareda, 1, E 18008 Granada, Spain
    Microb Biotechnol 1:87-93. 2008
    ..This study supports the hypothesis that the removal of persistent toxic chemicals can be accelerated by combinations of plants and bacteria, a process generally known as rhizoremediation...
  5. pmc Microbial responses to xenobiotic compounds. Identification of genes that allow Pseudomonas putida KT2440 to cope with 2,4,6-trinitrotoluene
    Matilde Fernandez
    Bio Iliberis Research and Development, Edificio BIC, Parque Tecnológico de Ciencias de la Salud, E 18100 Armilla, Granada, Spain
    Microb Biotechnol 2:287-94. 2009
    ..The ORF PP1232 whose transcriptional level did not change in response to TNT affected growth in the presence of nitroaromatic compounds and it was found in a screening of 4000 randomly generated mutants...
  6. pmc Physiological responses of Pseudomonas putida to formaldehyde during detoxification
    Amalia Roca
    Department of Environmental Protection, Consejo Superior de Investigaciones Cientificas, C Prof Albareda, 1, E 18008 Granada, Spain
    Microb Biotechnol 1:158-69. 2008
    ..Two mutants with insertions in recD and fhdA were found which were unable to grow in the presence of 1.5 mM HCOH. The recD mutant was hypersensitive to 10 mM HCOH and died at a higher rate than the parental strain...
  7. pmc Characterization of the RND family of multidrug efflux pumps: in silico to in vivo confirmation of four functionally distinct subgroups
    Patricia Godoy
    Consejo Superior de Investigaciones Cientificas, Department of Environmental Protection, E 18008 Granada, Spain
    Microb Biotechnol 3:691-700. 2010
    ....
  8. doi request reprint Laboratory research aimed at closing the gaps in microbial bioremediation
    Juan Luis Ramos
    CSIC, Estacion Experimental del Zaidin, Granada, Spain
    Trends Biotechnol 29:641-7. 2011
    ..Current 'omics' technologies allow the exploitation of the catabolic potential of microbes without the need to cultivate them. Synthetic microbiology builds new catabolic pathways to remove recalcitrant pollutants from the environment...
  9. pmc Genomic analysis reveals the major driving forces of bacterial life in the rhizosphere
    Miguel A Matilla
    Department of Environmental Protection, Estación Experimental de Zaidín, Consejo Superior de Investigaciones Cientificas, Profesor Albareda 1, Granada 18008, Spain
    Genome Biol 8:R179. 2007
    ..Mutualistic interactions less well known than those between rhizobia and legumes are commonly found between plants and bacteria, frequently pseudomonads, which colonize roots and adjacent soil areas (the rhizosphere)...
  10. doi request reprint Responses of Pseudomonas to small toxic molecules by a mosaic of domains
    Juan L Ramos
    Consejo Superior de Investigaciones Científica, Estacion Experimental del Zaidin, Department of Environmental Protection, Granada, Spain
    Curr Opin Microbiol 12:215-20. 2009
    ..A mosaic of domains has been recruited to sense and metabolize these chemicals in order to obtain energy and carbon...
  11. ncbi request reprint Bioremediation of polynitrated aromatic compounds: plants and microbes put up a fight
    Juan L Ramos
    Estacion Experimental del Zaidin, Consejo Superior de Investigaciones Cientificas, Apdo Correos 419, E 18008 Granada, Spain
    Curr Opin Biotechnol 16:275-81. 2005
    ..The most effective in situ biotreatments for TNT are the use of bioslurry (which can be preceded by an abiotic step) and phytoremediation. Phytoremediation can be enhanced by using transgenic plants alone or together with microbes...
  12. pmc The TetR family of transcriptional repressors
    Juan L Ramos
    Department of Plant Biochemistry and Molecular and Cellular Biology, Estacion Experimental del Zaidin, Consejo Superior de Investigaciones Cientificas, Granada, Spain
    Microbiol Mol Biol Rev 69:326-56. 2005
    ..Information related to the TetR family of regulators has been updated in a database that can be accessed at www.bactregulators.org...
  13. ncbi request reprint Transcriptional control of the Pseudomonas TOL plasmid catabolic operons is achieved through an interplay of host factors and plasmid-encoded regulators
    J L Ramos
    Consejo Superior de Investigaciones Cientificas, Department of Biochemistry and Molecular and Cellular Biology of Plants, Granada, Spain
    Annu Rev Microbiol 51:341-73. 1997
    ..The two sigma54-dependent promoters are additionally subject to global (catabolite repression) control...
  14. ncbi request reprint Responses of Gram-negative bacteria to certain environmental stressors
    J L Ramos
    Consejo Superior de Investigaciones Cientificas, Estación, Experimental del Zaidin, Department of Plant Biochemistry, Profesor Albareda 1, 18008, Granda, Spain
    Curr Opin Microbiol 4:166-71. 2001
    ..Both cold shock and heat shock responses are mainly regulated at a post-transcriptional level, translation efficiency in the case of cold shock and mRNA half-life and sigma32 stability in the case of heat shock...
  15. ncbi request reprint Metabolism of nitrate esters by a consortium of two bacteria
    J L Ramos
    Consejo Superior de Investigaciones Cientificas, Department of Biochemistry and Molecular and Cellular Biology of Plants
    Nat Biotechnol 14:320-2. 1996
    ..Waters polluted with ethylene glycol dinitrate have been shown amenable to biological treatment in a pilot plant with wastewaters generated during the synthesis of the chemical in a factory...
  16. ncbi request reprint Mechanisms of solvent tolerance in gram-negative bacteria
    Juan L Ramos
    Department of Plant Biochemistry and Molecular and Cellular Biology, Estacion Experimental del Zaidin, Consejo Superior de Investigaciones Cientificas, E 18008 Granada, Spain
    Annu Rev Microbiol 56:743-68. 2002
    ..Specific and global regulators control the expression of the efflux pump operons of E. coli and P. putida at the transcriptional level...
  17. pmc Antibiotic-dependent induction of Pseudomonas putida DOT-T1E TtgABC efflux pump is mediated by the drug binding repressor TtgR
    Wilson Teran
    Consejo Superior de Investigaciones Cientificas, Estacion Experimental del Zaidin, Department of Plant Biochemistry, Molecular and Cellular Biology, Granada, Spain
    Antimicrob Agents Chemother 47:3067-72. 2003
    ..This is the first case in which the expression of a drug transporter of the resistance-nodulation-division family has been shown to be regulated directly by antibiotics...
  18. pmc Domain cross-talk during effector binding to the multidrug binding TTGR regulator
    Craig Daniels
    Department of Environmental Protection, CSIC, E 18008 Granada, Spain
    J Biol Chem 285:21372-81. 2010
    ....
  19. doi request reprint Gef gene therapy enhances the therapeutic efficacy of doxorubicin to combat growth of MCF-7 breast cancer cells
    Jose Prados
    Department of Human Anatomy and Embryology, School of Medicine, Institute of Biopathology and Regenerative Medicine, University of Granada, 18071, Granada, Spain
    Cancer Chemother Pharmacol 66:69-78. 2010
    ....
  20. ncbi request reprint Optimization of the palindromic order of the TtgR operator enhances binding cooperativity
    Tino Krell
    Department of Environmental Protection, Estacion Experimental del Zaidin, Consejo Superior de Investigaciones Cientificas, Apartado de Correos 419, 18008 Granada, Spain
    J Mol Biol 369:1188-99. 2007
    ..Based on the architecture of the operator, the thermodynamics of the process, and the TtgR-operator interactions we propose a model for the binding of TtgR to its target sequence...
  21. pmc High specificity in CheR methyltransferase function: CheR2 of Pseudomonas putida is essential for chemotaxis, whereas CheR1 is involved in biofilm formation
    Cristina García-Fontana
    Department of Environmental Protection, Estacion Experimental del Zaidin, Consejo Superior de Investigaciones Cientificas, C Prof Albareda, 1, 18008 Granada, Spain
    J Biol Chem 288:18987-99. 2013
    ..We conclude that CheR2 forms part of a chemotaxis pathway, and CheR1 forms part of a chemosensory route that controls biofilm formation. Data suggest that CheR methyltransferases act with high specificity on their cognate chemoreceptors...
  22. doi request reprint Responses of Pseudomonas putida to toxic aromatic carbon sources
    Tino Krell
    Department of Environmental Protection, Estacion Experimental del Zaidin, Consejo Superior de Investigaciones Cientificas, C Prof Albareda 1, Granada, Spain
    J Biotechnol 160:25-32. 2012
    ..Data suggest that transcriptional activation of the TOD pathway occurs at very low toluene concentrations whereas TtgV mediated induction of pump expression sets in as the toluene concentration further increases...
  23. doi request reprint Analysis of the plant growth-promoting properties encoded by the genome of the rhizobacterium Pseudomonas putida BIRD-1
    Amalia Roca
    Polígono Industrial Juncaril, Bio Iliberis R and D, 18210, Peligros, Granada, Spain
    Environ Microbiol 15:780-94. 2013
    ..BIRD-1 overproduces indole-3-acetic acid through convergent pathways...
  24. ncbi request reprint Plasmolysis induced by toluene in a cyoB mutant of Pseudomonas putida
    Estrella Duque
    Consejo Superior de Investigaciones Cientificas, Estacion Experimental del Zaidin, Department of Plant Biochemistry and Molecular and Cellular Biology, E 18008 Granada, Spain
    Environ Microbiol 6:1021-31. 2004
    ....
  25. doi request reprint Identification of reciprocal adhesion genes in pathogenic and non-pathogenic Pseudomonas
    Estrella Duque
    Consejo Superior de Investigaciones Científicas EEZ, Department of Environmental Protection, 18008 Granada, Spain
    Environ Microbiol 15:36-48. 2013
    ..In silico analysis showed this set of genes to be well conserved in all sequenced P. putida strains, and that at least eight reciprocal genes involved in attachment are shared by P. putida and P. aeruginosa...
  26. doi request reprint Regression of established subcutaneous B16-F10 murine melanoma tumors after gef gene therapy associated with the mitochondrial apoptotic pathway
    Jose Prados
    Institute of Biopathology and Regenerative Medicine IBIMER, Granada, Spain
    Exp Dermatol 19:363-71. 2010
    ..Our in vivo results indicate that gef gene might become a suitable therapeutic strategy for patients with advanced melanoma...
  27. ncbi request reprint Transcriptional tradeoff between metabolic and stress-response programs in Pseudomonas putida KT2440 cells exposed to toluene
    Patricia Domínguez-Cuevas
    Department of Biochemistry and Molecular and Cellular Biology of Plants, Estacion Experimental del Zaidin, Consejo Superior de Investigaciones Cientificas, Profesor Albareda, 1, E 18008 Granada, Spain
    J Biol Chem 281:11981-91. 2006
    ..putida KT2440 as a stressor rather than as a nutrient and that the inhibition by the aromatic compounds of many functions we tested is the tradeoff for activating stress tolerance genes at a minimal cost in terms of energy...
  28. doi request reprint The pGRT1 plasmid of Pseudomonas putida DOT-T1E encodes functions relevant for survival under harsh conditions in the environment
    Lazaro Molina
    Environmental Protection Department, Estacion Experimental del Zaidin, CSIC, C Profesor Albareda 1, 18008 Granada, Spain
    Environ Microbiol 13:2315-27. 2011
    ..Furthermore, this plasmid encodes functions that allow the cells to chemotactically respond to toluene and participate in iron scavenging...
  29. doi request reprint PhhR binds to target sequences at different distances with respect to RNA polymerase in order to activate transcription
    M Carmen Herrera
    Department of Environmental Protection, Estacion Experimental del Zaidin, CSIC, C Profesor Albareda 1, Granada, Spain
    J Mol Biol 394:576-86. 2009
    ..When functioning as an enhancer protein, PhhR-mediated transcription is modulated by the integration host factor protein. PhhR represses transcription from its own promoter and the promoter of the paaY gene by steric hindrance...
  30. pmc Subfunctionality of hydride transferases of the old yellow enzyme family of flavoproteins of Pseudomonas putida
    Pieter van Dillewijn
    Departamento de Protección Ambiental, Estación del Zaidín, Consejo Superior de Investigaciones Cientificas, Granada, Spain
    Appl Environ Microbiol 74:6703-8. 2008
    ....
  31. pmc The RpoT regulon of Pseudomonas putida DOT-T1E and its role in stress endurance against solvents
    Estrella Duque
    CSIC Estacion Experimental del Zaidin, Department of Environmental Protection, Profesor Albareda, 1, E 18008 Granada, Spain
    J Bacteriol 189:207-19. 2007
    ..Hypersensitivity of the P. putida RpoT-deficient mutant to organic solvents can be attributed to the fact that in the DeltarpoT strain, expression of the toluene efflux pump ttgGHI genes is severalfold lower than in the parental strain...
  32. ncbi request reprint 16S rDNA phylogeny and distribution of lin genes in novel hexachlorocyclohexane-degrading Sphingomonas strains
    Dietmar Böltner
    Department of Plant Biochemistry and Molecular and Cellular Biology, Estacion Experimental del Zaidin, CSIC, Calle Profesor Albareda 1, Granada 18008, Spain
    Environ Microbiol 7:1329-38. 2005
    ..The loss of the ability to degrade gamma-HCH was associated with the deletion of the linA gene, probably due to recombination involving IS6100 elements, of which several copies are located in the lin cluster region...
  33. ncbi request reprint Effector-repressor interactions, binding of a single effector molecule to the operator-bound TtgR homodimer mediates derepression
    Wilson Teran
    Department of Biochemistry, Estacion Experimental del Zaidin, Consejo Superior de Investigaciones Cientificas, Apartado de Correos 419, E 18008 Granada, Spain
    J Biol Chem 281:7102-9. 2006
    ..This provides important insight into the molecular mechanism of effector-mediated derepression...
  34. pmc Involvement of cyclopropane fatty acids in the response of Pseudomonas putida KT2440 to freeze-drying
    Jesús Muñoz-Rojas
    Estacion Experimental del Zaidin, Consejo Superior de Investigaciones Cientificas, C Prof Albareda 1, 18008 Granada, Spain
    Appl Environ Microbiol 72:472-7. 2006
    ..These mutants were more sensitive to freeze-drying than wild-type cells, particularly the mutant with a knockout in the cfaB gene that produced less than 2% of the amount of C17:cyclopropane produced by the parental strain...
  35. doi request reprint Solvent tolerance in Gram-negative bacteria
    Ana Segura
    Consejo Superior de Investigaciones Cientificas, Estacion Experimental del Zaidin, Department of Environmental Protection, E 18008 Granada, Spain
    Curr Opin Biotechnol 23:415-21. 2012
    ..We discuss the recent advances in our understanding of the mechanisms involved in solvent tolerance...
  36. pmc Mechanisms of resistance to chloramphenicol in Pseudomonas putida KT2440
    Matilde Fernandez
    Bio Iliberis Research and Development, I D Department, Peligros, Granada, Spain
    Antimicrob Agents Chemother 56:1001-9. 2012
    ..Transcriptional arrays revealed that AgmR controls the expression of the pqq genes and the operon encoding the ABC extrusion pump from the promoter upstream of open reading frame (ORF) PP2669...
  37. pmc Compartmentalized glucose metabolism in Pseudomonas putida is controlled by the PtxS repressor
    Abdelali Daddaoua
    Department of Environmental Protection, Consejo Superior de Investigaciones Cientificas, Calle Profesor Albareda 1, Granada, Spain
    J Bacteriol 192:4357-66. 2010
    ..Footprint analysis revealed that PtxS binds to an almost perfect palindrome that is present within the three promoters and whose consensus sequence is 5'-TGAAACCGGTTTCA-3'. This palindrome overlaps with the RNA polymerase binding site...
  38. pmc Metabolic potential of the organic-solvent tolerant Pseudomonas putida DOT-T1E deduced from its annotated genome
    Zulema Udaondo
    Estación Experimental del Zadín CSIC, Profesor Albareda 1, 18008 Granada, Spain
    Microb Biotechnol 6:598-611. 2013
    ....
  39. pmc Biotransformation in double-phase systems: physiological responses of Pseudomonas putida DOT-T1E to a double phase made of aliphatic alcohols and biosynthesis of substituted catechols
    Antonia Rojas
    Department of Biochemistry and Molecular and Cellular Biology of Plants, CSIC Estacion Experimental del Zaidin, E 18008 Granada, Spain
    Appl Environ Microbiol 70:3637-43. 2004
    ..8). The amount of 3-methylcatechol produced in an aliphatic alcohol/water bioreactor was 10- to 20-fold higher than in an aqueous medium, demonstrating the usefulness of double-phase systems for this particular biotransformation...
  40. doi request reprint Paralogous chemoreceptors mediate chemotaxis towards protein amino acids and the non-protein amino acid gamma-aminobutyrate (GABA)
    Miriam Rico-Jiménez
    Department of Environmental Protection, Estacion Experimental del Zaidin, Consejo Superior de Investigaciones Cientificas, C Prof Albareda, 1, 18008, Granada, Spain
    Mol Microbiol 88:1230-43. 2013
    ....
  41. ncbi request reprint Fatty acid biosynthesis is involved in solvent tolerance in Pseudomonas putida DOT-T1E
    Ana Segura
    Department of Biochemistry and Molecular and Cellular Biology of Plants, CSIC Estacion Experimental del Zaidin, E 18008 Granada, Spain
    Environ Microbiol 6:416-23. 2004
    ..The mutation in P. putida DOT-T1E-109 increases sensitivity to the solvent because of a limited efflux of the solvent from the cell membranes with the concomitant inhibition of fatty acid biosynthesis...
  42. ncbi request reprint Detection of multiple extracytoplasmic function (ECF) sigma factors in the genome of Pseudomonas putida KT2440 and their counterparts in Pseudomonas aeruginosa PA01
    Manuel A Martínez-Bueno
    Department of Biochemistry and Molecular and Cellular Biology of Plants, Estacion Experimental del Zaidin, Consejo Superior de Investigaciones Cientificas, C Prof Albareda, 1, 18008 Granada, Spain
    Environ Microbiol 4:842-55. 2002
    ..This may explain the ability of P. putida KT2440 to grow under low iron availability conditions. Five fecI/fecR/iron receptor gene clusters from P. putida were also identified in the human pathogen Pseudomonas aeruginosa...
  43. ncbi request reprint OYE flavoprotein reductases initiate the condensation of TNT-derived intermediates to secondary diarylamines and nitrite
    Rolf Michael Wittich
    Departamento de Protección Ambiental, Estacion Experimental del Zaidin, Consejo Superior de Investigaciones Cientificas, Calle Profesor Albareda 1, E 18008 Granada, Spain
    Environ Sci Technol 42:734-9. 2008
    ..The structures of the formed end products were unequivocally elucidated by NMR...
  44. pmc Study of the TmoS/TmoT two-component system: towards the functional characterization of the family of TodS/TodT like systems
    Hortencia Silva-Jímenez
    Department of Environmental Protection, Estacion Experimental del Zaidin, Consejo Superior de Investigaciones Cientificas, C Prof Albareda 1, Granada, Spain
    Microb Biotechnol 5:489-500. 2012
    ..Information on the TmoS/TmoT and TodS/TodT system combined with a sequence analysis of family members permits to identify distinct features that define this protein family...
  45. pmc Enhanced tolerance to naphthalene and enhanced rhizoremediation performance for Pseudomonas putida KT2440 via the NAH7 catabolic plasmid
    Matilde Fernandez
    Bio Iliberis Research and Development, Granada, Spain
    Appl Environ Microbiol 78:5104-10. 2012
    ....
  46. doi request reprint Analysis of solvent tolerance in Pseudomonas putida DOT-T1E based on its genome sequence and a collection of mutants
    Zulema Udaondo
    Consejo Superior de Investigaciones Cientificas, EEZ, Department of Environmental Protection, 18008 Granada, Spain
    FEBS Lett 586:2932-8. 2012
    ..Local and global transcriptional regulators participate in a complex network of metabolic functions, acting as the decision makers in the response to solvents...
  47. doi request reprint Physiologically relevant divalent cations modulate citrate recognition by the McpS chemoreceptor
    Jesús Lacal
    Department of Environmental Protection, Estacion Experimental del Zaidin, Consejo Superior de Investigaciones Cientificas, C Profesor Albareda, 1, Granada 18008, Spain
    J Mol Recognit 24:378-85. 2011
    ..However, this reduction is much less important in the presence of citrate-Mg(2+) complexes. The physiological relevance of these findings is discussed...
  48. doi request reprint Bacterial sensor kinases: diversity in the recognition of environmental signals
    Tino Krell
    Department of Environmental Protection, Estacion Experimental del Zaidin, Consejo Superior de Investigaciones Cientificas, 18008 Granada, Spain
    Annu Rev Microbiol 64:539-59. 2010
    ..The biochemical data available on SKs, particularly their molecular interactions with signals, nucleotides, and their cognate RRs, are also reviewed...
  49. pmc Proteomic analysis reveals the participation of energy- and stress-related proteins in the response of Pseudomonas putida DOT-T1E to toluene
    Ana Segura
    EEZ CSIC, C Prof Albareda, 1, E 18008 Granada, Spain
    J Bacteriol 187:5937-45. 2005
    ....
  50. pmc Cell density-dependent gene contributes to efficient seed colonization by Pseudomonas putida KT2440
    Manuel Espinosa-Urgel
    Department of Plant Biochemistry and Molecular and Cellular Biology, Estacion Experimental del Zaidin, CSIC, Granada 18008, Spain
    Appl Environ Microbiol 70:5190-8. 2004
    ..This is the first evidence suggesting the existence of a quorum-sensing system in P. putida KT2440. The potential implication of such a signaling process in seed adhesion and colonization by the bacterium is discussed...
  51. ncbi request reprint REP code: defining bacterial identity in extragenic space
    Raquel Tobes
    Consejo Superior de Investigaciones Cientificas, Estación Experimental del Zaidín Department of Plant Biochemistry and Molecular and Cellular Biology Profesor Albareda number 1, E 18008 Granada, Spain
    Environ Microbiol 7:225-8. 2005
    ....
  52. doi request reprint Transcriptional control of the main aromatic hydrocarbon efflux pump in Pseudomonas
    Sandy Fillet
    Consejo Superior de Investigaciones Cientificas, EEZ, Department of Environmental Protection, Granada, Spain University of Toronto, Department of Banting and Best Department of Medical Research, Toronto, Canada Imperial College of Science and Technology, London, UK
    Environ Microbiol Rep 4:158-67. 2012
    ..The most relevant element in this toluene tolerance arsenal is the TtgGHI efflux pump controlled by the TtgV regulator. We discuss here how TtgV controls expression of this efflux pump in response to solvents...
  53. pmc Sequential XylS-CTD binding to the Pm promoter induces DNA bending prior to activation
    Patricia Domínguez-Cuevas
    Department of Environmental Protection, Estacion Experimental del Zaidin, Consejo Superior de Investigaciones Cientificas, C Profesor Albareda no 1, E 18008 Granada, Spain
    J Bacteriol 192:2682-90. 2010
    ..We propose a model in which DNA structure and binding sequences strongly influence XylS binding events previous to transcription activation...
  54. pmc Global regulation of food supply by Pseudomonas putida DOT-T1E
    Craig Daniels
    Department of Environmental Protection, EEZ CSIC, C Profesor Albareda, 1, E 18008 Granada, Spain
    J Bacteriol 192:2169-81. 2010
    ....
  55. pmc Transcriptional control by two interacting regulatory proteins: identification of the PtxS binding site at PtxR
    Abdelali Daddaoua
    Department of Environmental Protection, Consejo Superior de Investigaciones Cientificas, Estacion Experimental del Zaidin, Calle Profesor Albareda 1, E 18008 Granada, Spain
    Nucleic Acids Res 41:10150-6. 2013
    ..In vitro transcription showed that PtxS per se is sufficient to inhibit transcription from the Pgad promoter, but that affinity of PtxS for its effector is modulated by PtxR. ..
  56. pmc Antibiotic resistance determinants in a Pseudomonas putida strain isolated from a hospital
    Lazaro Molina
    Laboratorio de Investigación y Control Agroalimentario, Universidad de Huelva, Huelva, Spain Estación Experimental del Zaidín, Consejo Superior de Investigaciones Cientificas, Granada, Spain Centro de Investigación en Química Sostenible, Universidad de Huelva, Huelva, Spain
    PLoS ONE 9:e81604. 2014
    ..The pPC9 plasmid is not self-transmissible, but can be mobilized by other bacterial plasmids making it capable of spreading antibiotic resistant determinants to new hosts. ..
  57. pmc In vivo gene expression of Pseudomonas putida KT2440 in the rhizosphere of different plants
    Matilde Fernandez
    Bio Iliberis Research and Development, I D Department, 18210, Peligros, Granada, Spain
    Microb Biotechnol 6:307-13. 2013
    ..The results obtained in this study suggest that plant-specific signals are sensed by KT2440 in the rhizosphere and that the signals and consequent gene expression are related to the bacteria's successful establishment in this niche...
  58. ncbi request reprint Growth phase-dependent expression of the Pseudomonas putida KT2440 transcriptional machinery analysed with a genome-wide DNA microarray
    Luis Yuste
    Departamento de Biotecnologia Microbiana, Centro Nacional de Biotecnologia, CSIC, Campus de la Universidad Autonoma de Madrid, Cantoblanco, 28049 Madrid, Spain
    Environ Microbiol 8:165-77. 2006
    ..Expression of IHF was indicative of post-transcriptional control. These results provide the first global study of the expression of the transcriptional machinery through the exponential stationary-phase shift in P. putida...
  59. doi request reprint Construction of a prototype two-component system from the phosphorelay system TodS/TodT
    Hortencia Silva-Jímenez
    Department of Environmental Protection, Estacion Experimental del Zaidin, Consejo Superior de Investigaciones Cientificas, C Prof Albareda, 1, 18008 Granada, Spain
    Protein Eng Des Sel 25:159-69. 2012
    ..Data will be discussed in the context that the phosphorelay system appears to be better suited for the control of a degradation pathway for toxic compounds...
  60. pmc Regulation of glucose metabolism in Pseudomonas: the phosphorylative branch and entner-doudoroff enzymes are regulated by a repressor containing a sugar isomerase domain
    Abdelali Daddaoua
    Department of Environmental Protection, Consejo Superior de Investigaciones Cientificas, Estacion Experimental del Zaidin, C Profesor Albareda 1, E 18008 Granada, Spain
    J Biol Chem 284:21360-8. 2009
    ....
  61. doi request reprint Regulation of the cyclopropane synthase cfaB gene in Pseudomonas putida KT2440
    Cecilia Pini
    Consejo Superior de Investigaciones Cient ıficas, Environmental Protection Department, Estacio n Experimental del Zaid ın, Granada, Spain
    FEMS Microbiol Lett 321:107-14. 2011
    ..In this regard, in a cti-deficient mutant background, the levels of CFAs were similar to those in the parental one under the same conditions...
  62. pmc Cyclopropane fatty acids are involved in organic solvent tolerance but not in acid stress resistance in Pseudomonas putida DOT-T1E
    Cecilia Vanesa Pini
    Environmental Protection Department, Estacion Experimental del Zaidin, Consejo Superior de Investigaciones Cientificas, C Profesor Albareda 1, 18008 Granada, Spain
    Microb Biotechnol 2:253-61. 2009
    ..putida cfaB mutant was as tolerant to acid shock as the wild-type strain. The cfaB mutant was also as tolerant as the parental strain to a number of drugs, antibiotics and other damaging agents...
  63. doi request reprint Diversity at its best: bacterial taxis
    Tino Krell
    Department of Environmental Protection, Estacion Experimental del Zaidin, Consejo Superior de Investigaciones Cientificas, C Prof Albareda 1, Granada, Spain
    Environ Microbiol 13:1115-24. 2011
    ..However, the physiological reasons for the majority of differences in the taxis systems are poorly understood and its elucidation represents a major research need...
  64. pmc Type II hydride transferases from different microorganisms yield nitrite and diarylamines from polynitroaromatic compounds
    Pieter van Dillewijn
    Departamento de Protección Ambiental, Estacion Experimental del Zaidin, Consejo Superior de Investigaciones Cientificas, Granada, Spain
    Appl Environ Microbiol 74:6820-3. 2008
    ....
  65. ncbi request reprint Catabolism of phenylalanine by Pseudomonas putida: the NtrC-family PhhR regulator binds to two sites upstream from the phhA gene and stimulates transcription with sigma70
    M Carmen Herrera
    Department of Environmental Protection, Estacion Experimental del Zaidin, Consejo Superior de Investigaciones Cientificas, Profesor Albareda 1, 18008 Granada, Spain
    J Mol Biol 366:1374-86. 2007
    ..An IHF binding site overlaps the proximal PhhR proximal motif, which is recognized by IHF with a K(D) of around 1.2 microM. IHF may consequently compete with PhhR for binding and indeed inhibits PhhR-dependent phhAB operon expression...
  66. doi request reprint Physiological and transcriptomic characterization of a fliA mutant of Pseudomonas putida KT2440
    Jose Juan Rodriguez-Herva
    Department of Environmental Protection, Estacion Experimental del Zaidin, Consejo Superior de Investigaciones Cientificas, E 18008 Granada, Spain Department of Molecular Biology, Umea University, S 901 87 Umea, Sweden Unidad Asociada de Contaminación Atmosférica, CSIC Universidad de Huelva, Huelva, Spain
    Environ Microbiol Rep 2:373-80. 2010
    ..putidaσ(28) recognizes a TCAAG-t-N12 -GCCGATA consensus sequence located between -34 and -8 and that this sequence is preferentially associated with an AT-rich upstream region...
  67. ncbi request reprint XylS-Pm promoter interactions through two helix-turn-helix motifs: identifying XylS residues important for DNA binding and activation
    Patricia Domínguez-Cuevas
    Consejo Superior de Investigaciones Cientificas, Estacion Experimental del Zaidin, Department of Environmental Protection, E 18008 Granada, Spain
    J Mol Biol 375:59-69. 2008
    ..We propose a model in which a XylS dimer binds to the direct repeats in Pm in a head-to-tail conformation that allows the direct interaction of the XylS proximal subunit with the RNA polymerase sigma factor...
  68. ncbi request reprint A two-partner secretion system is involved in seed and root colonization and iron uptake by Pseudomonas putida KT2440
    Maria Antonia Molina
    Department of Plant Biochemistry and Molecular and Cellular Biology, Estacion Experimental del Zaidin, CSIC, Profesor Albareda, 1, Granada 18008, Spain
    Environ Microbiol 8:639-47. 2006
    ..These data further strengthen our previous observations indicating the importance of iron acquisition for attachment of P. putida KT2440 to plant surfaces...
  69. doi request reprint Taxonomic and functional metagenomic profiling of the microbial community in the anoxic sediment of a sub-saline shallow lake (Laguna de Carrizo, Central Spain)
    Manuel Ferrer
    CSIC, Institute of Catalysis, 28049 Madrid, Spain
    Microb Ecol 62:824-37. 2011
    ....
  70. pmc A two-component regulatory system integrates redox state and population density sensing in Pseudomonas putida
    Regina Fernández-Piñar
    Department of Environmental Protection, Estacion Experimental del Zaidin, Consejo Superior de Investigaciones Cientificas, Granada 18008, Spain
    J Bacteriol 190:7666-74. 2008
    ..A putative RoxR recognition element containing a conserved hexamer (TGCCAG) has also been identified in promoters of genes regulated by this two-component system...
  71. pmc The sensor kinase TodS operates by a multiple step phosphorelay mechanism involving two autokinase domains
    Andreas Busch
    Department of Environmental Protection, Estacion Experimental del Zaidin, Consejo Superior de Investigaciones Cientificas, C Prof Albareda, 1, 18008 Granada, Spain
    J Biol Chem 284:10353-60. 2009
    ..This is the first report of a multiple step phosphorelay mechanism of a sensor kinase that involves two autokinase domains...
  72. doi request reprint Sensing of environmental signals: classification of chemoreceptors according to the size of their ligand binding regions
    Jesús Lacal
    Department of Environmental Protection, Estacion Experimental del Zaidin, Consejo Superior de Investigaciones Cientificas, C Prof Albareda, 1, 18008 Granada, Spain
    Environ Microbiol 12:2873-84. 2010
    ..Topologies involving extracytoplasmic sensing are prevalent in bacteria whereas topologies with cytosolic signal recognition are abundant in archaea...
  73. pmc The IclR family of transcriptional activators and repressors can be defined by a single profile
    Tino Krell
    Estacion Experimental del Zaidin, Consejo Superior de Investigaciones Cientificas, 18008 Granada, Spain
    Protein Sci 15:1207-13. 2006
    ..More than 500 representatives of this family were identified with this profile. A database on bacterial regulators (http://www.bactregulators.org) was built to compile and regroup the sequences with the aid of the new profile...
  74. doi request reprint Functional analysis of new transporters involved in stress tolerance in Pseudomonas putida DOT-T1E
    Vanina García
    Environmental Protection Department, Estacion Experimental del Zaidin, Consejo Superior de Investigaciones Científicas C Profesor Albareda 1, 18008 Granada, Spain
    Environ Microbiol Rep 2:389-95. 2010
    ..putida DOT-T1E, while the other belongs to the Major Facilitator Superfamily and exhibits homology to a putative transporter of the Bcr/CflA family that has not previously been reported to be involved in toluene tolerance...
  75. pmc Involvement of the global Crp regulator in cyclic AMP-dependent utilization of aromatic amino acids by Pseudomonas putida
    M Carmen Herrera
    Consejo Superior de Investigaciones Cientificas, Estacion Experimental del Zaidin, Department of Environmental Protection, Granada, Spain
    J Bacteriol 194:406-12. 2012
    ..This nucleoprotein complex promotes transcription from phhA only in response to L-phenylalanine...
  76. pmc Complete genome of the plant growth-promoting rhizobacterium Pseudomonas putida BIRD-1
    Miguel A Matilla
    Bio Iliberis R and D, Polígono Industrial Juncaril, Granada, Spain
    J Bacteriol 193:1290. 2011
    ....
  77. ncbi request reprint Root colonization by Pseudomonas putida: love at first sight
    Manuel Espinosa-Urgel
    Department of Plant Biochemistry and Molecular and Cellular Biology, Estacin Experimental del Zaidn, CSIC, Profesor Albareda 1, Granada 18008, Spain
    Microbiology 148:341-3. 2002
  78. pmc Characterization of the Pseudomonas putida mobile genetic element ISPpu10: an occupant of repetitive extragenic palindromic sequences
    Maria Isabel Ramos-Gonzalez
    Department of Plant Biochemistry and Molecular and Cellular Biology, Estacion Experimental del Zaidin, CSIC, Profesor Albareda 1, Granada 18008, Spain
    J Bacteriol 188:37-44. 2006
    ..putida DOT-T1E, an ISPpu10-free strain with REPs similar to those of strain KT2440. Plasmid ISPpu10-mediated rearrangements were observed in vivo under laboratory conditions and in the plant rhizosphere...
  79. ncbi request reprint Microorganisms and explosives: mechanisms of nitrogen release from TNT for use as an N-source for growth
    Rolf Michael Wittich
    Departamento de Protección Ambiental, Estacion Experimental del Zaidin, Consejo Superior de Investigaciones Cientificas, Calle Profesor Albareda 1, E 18008 Granada, Spain
    Environ Sci Technol 43:2773-6. 2009
    ..Using uniformly 15N-labeled (15N3) TNT, we show that the nitrite is being released by the condensation reaction and, also under environmental conditions, will originate from the microbiologically generated dihydride complex...
  80. ncbi request reprint Combined therapy using suicide gef gene and paclitaxel enhances growth inhibition of multicellular tumour spheroids of A-549 human lung cancer cells
    Jose Prados
    Institute of Biopathology and Regenerative Medicine IBIMER, School of Medicine, University of Granada, Granada, Spain
    Int J Oncol 33:121-7. 2008
    ..In conclusion, gef gene has a cytotoxic effect in lung cancer cells and enhances cell growth inhibition when used with paclitaxel. These results indicate that this combined therapy may be of potential therapeutic value in lung cancer...
  81. doi request reprint Pseudomonas aeruginosa strain RW41 mineralizes 4-chlorobenzenesulfonate, the major polar by-product from DDT manufacturing
    Rafael Blasco
    Departamento de Bioquimica, Biología Molecular y Genética, Facultad de Veterinaria, Universidad de Extremadura, E 10071 Cáceres, Spain
    Environ Microbiol 10:1591-600. 2008
    ....