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Genomes and Genes | Amparo QuerolSummaryCountry: Spain Publications
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Publications
The complex and dynamic genomes of industrial yeastsAmparo Querol
Departamento de Biotecnologia, Instituto de Agroquimica y Tecnologia de los Alimentos CSIC, Burjasot, Valencia, Spain
FEMS Microbiol Lett 293:1-10. 2009..The polyploid and hybrid nature of the genomes confers robust characteristics such as tolerance to environmental stress to these industrial yeasts and provides a means for adaptive evolution...
Differences in the glucose and fructose consumption profiles in diverse Saccharomyces wine species and their hybrids during grape juice fermentationJordi Tronchoni
Departamento de Biotecnologia de Alimentos, Instituto de Agroquimica y Tecnologia de los Alimentos, CSIC P O Box 73, E 46100 Burjassot, Valencia, Spain
Int J Food Microbiol 134:237-43. 2009..This kind of studies can be very useful for the wine industry to select yeast strains with different glucose/fructose preferences...
Molecular characterization of new natural hybrids of Saccharomyces cerevisiae and S. kudriavzevii in brewingSara S González
Departamento de Biotecnologia, Instituto de Agroquimica y Tecnologia de Alimentos CSIC, P O Box 73, 46100 Burjassot, Valencia, Spain
Appl Environ Microbiol 74:2314-20. 2008..The present study raises new questions about the prevalence of these new hybrids in brewing as well as their contribution to the properties of the final product...
Adaptive evolution of wine yeastAmparo Querol
Departamento de Biotecnologia de Alimentos, Instituto de Agroquimica y Tecnologia de Alimentos, CSIC, P O Box 73, 46100 Burjassot, Valencia, Spain
Int J Food Microbiol 86:3-10. 2003..In this review, we describe the different mechanisms involved in the adaptive evolution of wine yeast strains...
Molecular evolution in yeast of biotechnological interestAmparo Querol
Departamento de Biotecnologia, Instituto de Agroquimica y Tecnologia de Alimentos, CSIC, P O Box 73, 46100, Burjassot, Valencia, Spain
Int Microbiol 6:201-5. 2003..This review describes the phylogenetic relationships among Saccharomyces and Kluyveromyces species and the different mechanisms involved in the adaptive evolution of industrial yeast strains...
Enological characterization of natural hybrids from Saccharomyces cerevisiae and S. kudriavzeviiSara S González
Departamento de Biotecnologia, Instituto de Agroquimica y Tecnologia de Alimentos CSIC, Apdo 73, 46100 Burjassot, Valencia, Spain
Int J Food Microbiol 116:11-8. 2007..The behavior displayed by these hybrids in the fermentations studied in this work leads us to conclude that the use of hybrid strains can constitute an advantage in wine making...
Fermentative stress adaptation of hybrids within the Saccharomyces sensu stricto complexCarmela Belloch
Departamento de Biotecnologia, Instituto de Agroquimica y Tecnologia de los Alimentos, CSIC, PO Box 73 E 46100 Burjassot, Valencia, Spain
Int J Food Microbiol 122:188-95. 2008..These results open new perspectives in the construction of new hybrid strains with biotechnological interest, as the characteristics of the parents may result in interesting combinations in the hybrids...
Natural hybrids of S. cerevisiae x S. kudriavzevii share alleles with European wild populations of Saccharomyces kudriavzeviiChristian A Lopes
Departamento de Biotecnologia, Instituto de Agroquimica y Tecnologia de los Alimentos, CSIC, Valencia, Spain
FEMS Yeast Res 10:412-21. 2010..kudriavzevii type strain, but identical to the alleles previously reported for natural S. cerevisiaexS. kudriavzevii hybrids. These results clearly support the hypothesis of a European origin for these natural hybrids...
Chimeric genomes of natural hybrids of Saccharomyces cerevisiae and Saccharomyces kudriavzeviiCarmela Belloch
Instituto de Agroquimica y Tecnologia de los Alimentos, IATA CSIC, P O Box 73, E 46100 Burjassot, Spain
Appl Environ Microbiol 75:2534-44. 2009..As a result of the selective processes acting during fermentation, hybrid genomes maintained the S. cerevisiae genome but reduced the S. kudriavzevii fraction...
Food and probiotic strains from the Saccharomyces cerevisiae species as a possible origin of human systemic infectionsRosa de Llanos
, , 46100 Burjassot, Valencia, Spain
Int J Food Microbiol 110:286-90. 2006..boulardii strain. The association of S. boulardii with cases of fungemia has been reported previously. Nevertheless, this is the first time that a baker's yeast has been isolated from blood...
Physiological characterization of spoilage strains of Zygosaccharomyces bailii and Zygosaccharomyces rouxii isolated from high sugar environmentsPatricia Martorell
Departamento de Biotecnologia, Instituto de Agroquimica y Tecnologia de Alimentos CSIC, P O Box 73, E 46100 Burjassot, Valencia, Spain
Int J Food Microbiol 114:234-42. 2007..These results indicate that spoilage by yeasts such as Z. bailii may be better prevented by use of biocidal cleaning agents in the factory, rather than treating the food with preservatives...
Identification of species in the genus Pichia by restriction of the internal transcribed spacers (ITS1 and ITS2) and the 5.8S ribosomal DNA geneMercedes Villa-Carvajal
, , CSIC, P.O. Box 73, 46100 Burjassot, Valencia, Spain
Antonie Van Leeuwenhoek 90:171-81. 2006..8S-ITS rDNA failed to differentiate between some Pichia and Candida species that could be distinguished on the basis of the sequence of the 5.8S-ITS rRNA region or the sequence of the D1/D2 domain of the 26S rDNA gene...
Differences in activation of MAP kinases and variability in the polyglutamine tract of Slt2 in clinical and non-clinical isolates of Saccharomyces cerevisiaeRosa de Llanos
Departamento de Biotecnologia, Instituto de Agroquimica y Tecnologia de los Alimentos, CSIC, PO Box 73, 46100 Burjassot, Valencia, Spain
Yeast 27:549-61. 2010..We suggest that this variability may influence biological features of the Slt2 protein, allowing it to adapt swiftly in order to survive in unusual environments...
Molecular characterization of clinical Saccharomyces cerevisiae isolates and their association with non-clinical strainsRosa de Llanos
, , Burjassot, Valencia, Spain
Syst Appl Microbiol 27:427-35. 2004..3% and 19.8% of the clinical isolates and there seemed to be a connection between their ability to colonize humans and their ability to cause vaginal infection. The rest of food isolates were not grouped with clinical strains...
Exclusion of Saccharomyces kudriavzevii from a wine model system mediated by Saccharomyces cerevisiaeF Noé Arroyo-López
Institut Cavanilles de Biodiversitat i Biologia Evolutiva, Universitat de Valencia, Edificio de Institutos Campus de Paterna, E 46100 Burjassot, Valencia, Spain
Yeast 28:423-35. 2011..cerevisiae × S. kudriavzevii hybrids most likely originated in wild environments rather than in industrial fermentations...
An analysis of inter- and intraspecific genetic variabilities in the Kluyveromyces marxianus group of yeast species for the reconsideration of the K. lactis taxonCarmela Belloch
, Universitat de Valencia, Calle Dr Moliner 50, 46100 Burjassot, Valencia, Spain
Yeast 19:257-68. 2002..These results suggest that the present taxon K. lactis is a complex of different species, subspecies or, at least, genetically structured populations...
Molecular characterisation of the species of the genus ZygosaccharomycesBraulio Esteve-Zarzoso
Departamento de Biotecnologia, Instituto de Agroquimica y Tecnologia de Alimentos (CSIC, Burjassot, Valincia, Spain
Syst Appl Microbiol 26:404-11. 2003..However, in the cases of Z. bailii and Z. lentus, electrophoretic karyotyping there was considerable variation...
Effect of aromatic precursor addition to wine fermentations carried out with different Saccharomyces species and their hybridsAmparo Gamero
Departamento de Biotecnologia, Instituto de Agroquímica y Tecnología de los Alimentos IATA, CSIC, Avda Agustín Escardino 7, 46980 Paterna, Valencia, Spain
Int J Food Microbiol 147:33-44. 2011..Altogether, these results suggest that it is possible to modulate wine aroma by employing different yeast species in order to create new wines with different aromatic notes...
Authentication and identification of Saccharomyces cerevisiae 'flor' yeast races involved in sherry ageingBraulio Esteve-Zarzoso
, , PO Box 73, 46100 Burjassot, , Spain; CECT (Spanish Type Culture Collection, , Burjassot, , Spain
Antonie Van Leeuwenhoek 85:151-8. 2004..8S rRNA pattern different from the rest of the Saccharomyces cerevisiae strains we propose that they should be included as a single race or variety inside the S. cerevisiae taxon...
Identification of species of the genus Candida by analysis of the 5.8S rRNA gene and the two ribosomal internal transcribed spacersRosa de Llanos Frutos
, , CSIC P.O. Box 73, 46100 Burjassot, Valencia, Spain
Antonie Van Leeuwenhoek 85:175-85. 2004..The present work improves the database available online at the IATA web page (http://motor.edinfo.es/iata/) with the patterns of 75 species belonging to the genus Candida...
Application of a substrate inhibition model to estimate the effect of fructose concentration on the growth of diverse Saccharomyces cerevisiae strainsF Noé Arroyo-López
Institut Cavanilles de Biodiversitat i Biologia Evolutiva, Universitat de Valencia, Edifici d Instituts, Parc Científic de Paterna, Valencia, Spain
J Ind Microbiol Biotechnol 36:663-9. 2009....
A comparison of clinical and food Saccharomyces cerevisiae isolates on the basis of potential virulence factorsRosa de Llanos
, , CSIC, P.O. Box 73, 46100, Burjassot, Valencia, Spain
Antonie Van Leeuwenhoek 90:221-31. 2006..cerevisiae (var. boulardii), were exceptions and based on their physiological traits these yeasts would appear to be related to clinical strains...
Molecular monitoring of spoilage yeasts during the production of candied fruit nougats to determine food contamination sourcesPatricia Martorell
, , P.O. Box 73, Burjassot E-46100, Valencia, Spain
Int J Food Microbiol 101:293-302. 2005..bailii and two strains of Z. rouxii were involved in the spoilage of candied fruits. Moreover, the Z. bailii strain was also present in the spoiled nougat, hence being responsible of this alteration...
Candida cabralensis sp. nov., a yeast species isolated from traditional Spanish blue-veined Cabrales cheeseAna Belén Flórez
Departamento de Microbiología y Bioquímica, Instituto de Productos Lacteos de Asturias, CSIC, Carretera de Infiesto s n, Asturias, Spain
Int J Syst Evol Microbiol 60:2671-4. 2010..The name Candida cabralensis sp. nov. is proposed, and the isolate 1AD8(T) (=CECT 13027(T) =CBS 11679(T)) is the type strain of this novel taxon...
Evaluation of different genetic procedures for the generation of artificial hybrids in Saccharomyces genus for winemakingLaura Pérez-Través
Departamento de Biotecnologia, Instituto de Agroquimica y Tecnologia de los Alimentos, CSIC, P O Box 73 E 46100 Burjassot, Valencia, Spain
Int J Food Microbiol 156:102-11. 2012..In this work, a wine yeast genetic stabilization process was developed and vegetatively stable hybrids were obtained...
Sequence-based identification of species belonging to the genus DebaryomycesPatricia Martorell
, , P.O. Box 73, E-46100 Burjassot, , Spain
FEMS Yeast Res 5:1157-65. 2005..robertsiae, D. udenii and D. vanrijiae showed high sequence similarity in ribosomal regions with one or several species. In these cases, sequence comparison of the ACT1 gene is proposed to ensure unequivocal strain designation...
Molecular typing of the yeast species Dekkera bruxellensis and Pichia guilliermondii recovered from wine related sourcesPatricia Martorell
, , P.O. Box 73, E-46100 Burjassot, , Spain
Int J Food Microbiol 106:79-84. 2006..The high degree of polymorphism observed shows that intraspecific typing is essential for accurate yeast dissemination studies in wine related environments...
Lipid composition of wine strains of Saccharomyces kudriavzevii and Saccharomyces cerevisiae grown at low temperatureJordi Tronchoni
Departamento de Biotecnología de los Alimentos, Instituto de Agroquimica y Tecnologia de Alimentos CSIC, P O Box 73, 46100 Burjassot, Valencia, Spain
Int J Food Microbiol 155:191-8. 2012..kudriavzevii at low temperatures. We have also confirmed the better fermentation performance of the strains of this species at low temperature, being an appealing alternative to S. cerevisiae for cold fermentations...
Molecular characterization of a chromosomal rearrangement involved in the adaptive evolution of yeast strainsJosé E Pérez-Ortín
Departament de Bioquimica i Biologia Molecular, Universitat de Valencia, Spain
Genome Res 12:1533-9. 2002..This is the first time that a gross chromosomal rearrangement is shown to be involved in the adaptive evolution of Saccharomyces cerevisiae...
Four new Candida cretensis strains isolated from Spanish fermented sausages (chorizo): taxonomic and phylogenetic implicationsManuel Quirós
Departamento de Microbiologia, Facultad de Biologia, Universidad Complutense de Madrid, Madrid, Spain
FEMS Yeast Res 8:485-91. 2008..cretensis according to the restriction patterns of the intergenic spacer rRNA gene region and on the basis of some physiological properties that are of ecological relevance...
Combined use of killer biotype and mtDNA-RFLP patterns in a Patagonian wine Saccharomyces cerevisiae diversity studyChristian A Lopes
, , , Universidad Nacional del Comahue, Buenos Aires 1400, , Argentina
Antonie Van Leeuwenhoek 89:147-56. 2006..The combined use of the mtDNA-RFLP analysis together with the killer biotype method proved to be a powerful tool in the fingerprinting of the enological S. cerevisiae strains...
Candida patagonica sp. nov., a new species of yeast from cellar surfacesMarcela P Sangorrín
Departamento de Quimica, Laboratorio de Microbiología y Biotecnología, Facultad de Ingenieria, Universidad Nacional del Comahue, Buenos Aires 1400, 8300, Neuquen, Argentina
Antonie Van Leeuwenhoek 92:77-81. 2007..All these data support the hypothesis that the new yeast, named Candida patagonica, is a novel species related to C. bituminiphila. The type strain is UNCOMA 159.5 (= CECT 12029 = CBS 10443)...
Patagonian wines: the selection of an indigenous yeast starterChristian A Lopes
Departamento de Quimica, Laboratorio de Microbiología y Biotecnología, Facultad de Ingenieria, Universidad Nacional del Comahue, Buenos Aires 1400, 8300 Neuquen, Argentina
J Ind Microbiol Biotechnol 34:539-46. 2007..cerevisiae killer strains assayed. Red wines with differential volatile profiles and interesting enological features were obtained at laboratory scale by using this selected indigenous strain...
Patagonian wines: implantation of an indigenous strain of Saccharomyces cerevisiae in fermentations conducted in traditional and modern cellarsChristian A Lopes
Departamento de Quimica, Laboratorio de Microbiología y Biotecnología, Facultad de Ingenieria, Universidad Nacional del Comahue, Buenos Aires 1400, 8300, Neuquen, Argentina
J Ind Microbiol Biotechnol 34:139-49. 2007..Therefore, MMf9 selected indigenous strain could be an interesting yeast starter culture in North Patagonian wines...
