J F Martin

Summary

Country: Spain

Publications

  1. ncbi request reprint Cloning, expression in Streptomyces lividans and biochemical characterization of a thermostable endo-beta-1,4-xylanase of Thermomonospora alba ULJB1 with cellulose-binding ability
    J Blanco
    Faculty of Biology, University of Leon, Spain
    Appl Microbiol Biotechnol 48:208-17. 1997
  2. ncbi request reprint Characterization and nitrogen-source regulation at the transcriptional level of the gdhA gene of Aspergillus awamori encoding an NADP-dependent glutamate dehydrogenase
    R E Cardoza
    Instituto de Biotecnologia INBIOTEC, Parque Cientifico de Leon, Avda del Real, 1, E 24006 León, Spain
    Curr Genet 34:50-9. 1998
  3. ncbi request reprint Characterization of the reverse transsulfuration gene mecB of Acremonium chrysogenum, which encodes a functional cystathionine-gamma-lyase
    A T Marcos
    Instituto de Biotecnologia INBIOTEC, Parque Cientifico de Leon, Spain
    Mol Gen Genet 264:746-54. 2001
  4. doi request reprint Transcriptomic studies of phosphate control of primary and secondary metabolism in Streptomyces coelicolor
    Juan F Martin
    Área de Microbiología Fac CC Biológicas y Ambientales, Universidad de Leon, Campus de Vegazana, 24071 Leon, Spain
    Appl Microbiol Biotechnol 95:61-75. 2012
  5. ncbi request reprint New aspects of genes and enzymes for beta-lactam antibiotic biosynthesis
    J F Martin
    Faculty of Biology, University of Leon, Spain
    Appl Microbiol Biotechnol 50:1-15. 1998
  6. ncbi request reprint Penicillin and cephalosporin biosynthesis: mechanism of carbon catabolite regulation of penicillin production
    J F Martin
    Faculty of Biology, University of Leon, Spain
    Antonie Van Leeuwenhoek 75:21-31. 1999
  7. ncbi request reprint Unraveling the methionine-cephalosporin puzzle in Acremonium chrysogenum
    Juan F Martin
    Area of Microbiology, University of Leon, Spain
    Trends Biotechnol 20:502-7. 2002
  8. ncbi request reprint Ribosomal RNA and ribosomal proteins in corynebacteria
    Juan F Martin
    Instituto de Biotecnologia de Leon, Parque Cientifico de Leon, Avda del Real, No 1, 24006 Leon, Spain
    J Biotechnol 104:41-53. 2003
  9. ncbi request reprint Secretion systems for secondary metabolites: how producer cells send out messages of intercellular communication
    Juan F Martin
    Area de Microbiologia, Facultad de Ciencias Biologicas y Ambientales, Campus de Vegazana, S N, 24071 Leon, Spain
    Curr Opin Microbiol 8:282-93. 2005
  10. pmc Phosphate control of the biosynthesis of antibiotics and other secondary metabolites is mediated by the PhoR-PhoP system: an unfinished story
    Juan F Martin
    Area of Microbiology, University of Leon, 24006, Leon, Spain
    J Bacteriol 186:5197-201. 2004

Collaborators

Detail Information

Publications65

  1. ncbi request reprint Cloning, expression in Streptomyces lividans and biochemical characterization of a thermostable endo-beta-1,4-xylanase of Thermomonospora alba ULJB1 with cellulose-binding ability
    J Blanco
    Faculty of Biology, University of Leon, Spain
    Appl Microbiol Biotechnol 48:208-17. 1997
    ..The major degradation product was xylobiose with traces of xylose. The xylanase activity was thermostable, showing a good activity up to 95 degrees C, and had broad pH stability in the range from pH 4.0 to pH 10.0...
  2. ncbi request reprint Characterization and nitrogen-source regulation at the transcriptional level of the gdhA gene of Aspergillus awamori encoding an NADP-dependent glutamate dehydrogenase
    R E Cardoza
    Instituto de Biotecnologia INBIOTEC, Parque Cientifico de Leon, Avda del Real, 1, E 24006 León, Spain
    Curr Genet 34:50-9. 1998
    ..These results indicate that expression of the gdhA gene is subject to a strong nitrogen regulation at the transcriptional level...
  3. ncbi request reprint Characterization of the reverse transsulfuration gene mecB of Acremonium chrysogenum, which encodes a functional cystathionine-gamma-lyase
    A T Marcos
    Instituto de Biotecnologia INBIOTEC, Parque Cientifico de Leon, Spain
    Mol Gen Genet 264:746-54. 2001
    ..The availability of this gene provides a very useful tool for understanding the proposed role of cystathionine-gamma-lyase in splitting cystathionine to supply cysteine for cephalosporin biosynthesis...
  4. doi request reprint Transcriptomic studies of phosphate control of primary and secondary metabolism in Streptomyces coelicolor
    Juan F Martin
    Área de Microbiología Fac CC Biológicas y Ambientales, Universidad de Leon, Campus de Vegazana, 24071 Leon, Spain
    Appl Microbiol Biotechnol 95:61-75. 2012
    ..The PhoP regulation of several of these genes has been studied in detail using EMSA and DNase I footprinting studies as well as in vivo expression studies with reporter genes and RT-PCR transcriptomic analyses...
  5. ncbi request reprint New aspects of genes and enzymes for beta-lactam antibiotic biosynthesis
    J F Martin
    Faculty of Biology, University of Leon, Spain
    Appl Microbiol Biotechnol 50:1-15. 1998
    ..Several new beta-lactam antibiotics have been discovered in gram-positive and gram-negative bacteria that will provide new genes for combinatorial synthesis of new molecules...
  6. ncbi request reprint Penicillin and cephalosporin biosynthesis: mechanism of carbon catabolite regulation of penicillin production
    J F Martin
    Faculty of Biology, University of Leon, Spain
    Antonie Van Leeuwenhoek 75:21-31. 1999
    ..Using protein extracts from mycelia grown under carbon catabolite repressing or derepressing conditions DNA-binding proteins that interact with the bidirectional promoter region were purified to near homogeneity...
  7. ncbi request reprint Unraveling the methionine-cephalosporin puzzle in Acremonium chrysogenum
    Juan F Martin
    Area of Microbiology, University of Leon, Spain
    Trends Biotechnol 20:502-7. 2002
    ..Eliminating cystathionine-gamma-lyase prevents the enhancing precursor effect of methionine on cephalosporin-C production, and cystathionine-gamma-lyase overproduction in moderate doses increases cephalosporin-C formation...
  8. ncbi request reprint Ribosomal RNA and ribosomal proteins in corynebacteria
    Juan F Martin
    Instituto de Biotecnologia de Leon, Parque Cientifico de Leon, Avda del Real, No 1, 24006 Leon, Spain
    J Biotechnol 104:41-53. 2003
    ..Some specific ribosomal protein genes are located in a different cluster in C. glutamicum when compared with E. coli, indicating that the control of expression of these genes is different in E. coli and C. glutamicum...
  9. ncbi request reprint Secretion systems for secondary metabolites: how producer cells send out messages of intercellular communication
    Juan F Martin
    Area de Microbiologia, Facultad de Ciencias Biologicas y Ambientales, Campus de Vegazana, S N, 24071 Leon, Spain
    Curr Opin Microbiol 8:282-93. 2005
    ..Genetic improvement of the secretion systems of secondary metabolites in the producer strain has important industrial applications...
  10. pmc Phosphate control of the biosynthesis of antibiotics and other secondary metabolites is mediated by the PhoR-PhoP system: an unfinished story
    Juan F Martin
    Area of Microbiology, University of Leon, 24006, Leon, Spain
    J Bacteriol 186:5197-201. 2004
  11. ncbi request reprint Characterization of the lys2 gene of Penicillium chrysogenum encoding alpha-aminoadipic acid reductase
    J Casqueiro
    Area of Microbiology, Faculty of Biology, University of Leon, Institute of Biotechnology INBIOTEC Parque Científico de León, Spain
    Mol Gen Genet 259:549-56. 1998
    ..The lys2-encoded protein is a member of the aminoacyladenylate-forming enzyme family with a reductase domain in its C-terminal region...
  12. pmc The folate branch of the methionine biosynthesis pathway in Streptomyces lividans: disruption of the 5,10-methylenetetrahydrofolate reductase gene leads to methionine auxotrophy
    J Blanco
    Department of Ecology, Genetics and Microbiology, Faculty of Biology, University of Leon, Spain
    J Bacteriol 180:1586-91. 1998
    ..These results indicate that the folate branch is essential for methionine biosynthesis in streptomycetes, as occurs in enterobacteria...
  13. ncbi request reprint Expression of genes and processing of enzymes for the biosynthesis of penicillins and cephalosporins
    J F Martin
    Department of Ecology, Genetics and Microbiology, Faculty of Biology, University of Leon, Spain
    Antonie Van Leeuwenhoek 65:227-43. 1994
    ..The levels of the cefG transcript were very low as compared to those of pcbAB, pcbC and cefEF.(ABSTRACT TRUNCATED AT 400 WORDS)..
  14. ncbi request reprint Characterization and lysine control of expression of the lys1 gene of Penicillium chrysogenum encoding homocitrate synthase
    O Banuelos
    Area of Microbiology, Faculty of Biology, University of Leon, 24071, Leon, Spain
    Gene 226:51-9. 1999
    ....
  15. ncbi request reprint Cloning and characterization of a phosphate-regulated promoter involved in phosphate control of candicidin biosynthesis
    A Rebollo
    Departamento de Ecologia, Genética y Microbiología, Facultad de Biologia, Universidad de Leon, Spain
    Gene 79:47-58. 1989
    ..The '114-bp' fragment is A + T-rich (54%), as compared to the Streptomyces genome (70-73% GC). The presence of a phosphate control sequence (pcs) in the upstream region of the pabS gene is proposed...
  16. ncbi request reprint Three genes hrdB, hrdD and hrdT of Streptomyces griseus IMRU 3570, encoding sigma factor-like proteins, are differentially expressed under specific nutritional conditions
    A T Marcos
    Department of Ecology, Genetics and Microbiology, Faculty of Biology, University of Leon, Spain
    Gene 153:41-8. 1995
    ..The hrdT gene is weakly transcribed (1.5-kb RNA) under all conditions tested. The hrd-encoded sigma factors probably recognize actinomycetes promoters (SEP type) with E. coli-like consensus sequences...
  17. pmc A cluster of three genes (dapA, orf2, and dapB) of Brevibacterium lactofermentum encodes dihydrodipicolinate synthase, dihydrodipicolinate reductase, and a third polypeptide of unknown function
    A Pisabarro
    Department of Ecology, Genetics and Microbiology, Faculty of Biology, University of Leon, Spain
    J Bacteriol 175:2743-9. 1993
    ..The protein encoded by this open reading frame had little similarity to any protein in the data banks, and its function remains unknown. The three genes were translated in Escherichia coli, giving the corresponding polypeptides...
  18. pmc Targeted inactivation of the mecB gene, encoding cystathionine-gamma-lyase, shows that the reverse transsulfuration pathway is required for high-level cephalosporin biosynthesis in Acremonium chrysogenum C10 but not for methionine induction of the cephalo
    G Liu
    Area of Microbiology, Faculty of Biology, University of Leon, 24071 Leon, Spain
    J Bacteriol 183:1765-72. 2001
    ..Levels of methionine induction of the cephalosporin biosynthesis gene pcbC were identical in the parental strain and the mecB mutants, indicating that the induction effect is not mediated by cystathionine-gamma-lyase...
  19. pmc Nucleotide sequence of the threonine synthase (thrC) gene of Brevibacterium lactofermentum
    M Malumbres
    Facultad de Biologia, Universidad de Leon, Spain
    Nucleic Acids Res 16:9859. 1988
  20. ncbi request reprint Subcellular localization of the homocitrate synthase in Penicillium chrysogenum
    O Banuelos
    Area de Microbiologia, Facultad de Biologia, Universidad de Leon, 24071 Leon, Spain
    Mol Genet Genomics 266:711-9. 2002
    ..The difference in localization of homocitrate synthase between P. chrysogenum and S. cerevisiae suggests that this protein may play a regulatory function, in addition to its catalytic function, in S. cerevisiae but not in P. chrysogenum...
  21. pmc A gene encoding arginyl-tRNA synthetase is located in the upstream region of the lysA gene in Brevibacterium lactofermentum: regulation of argS-lysA cluster expression by arginine
    J A Oguiza
    Area of Microbiology, Faculty of Biology, University of Leon, Spain
    J Bacteriol 175:7356-62. 1993
    ..The transcription of these genes is repressed by lysine and induced by arginine, showing an interesting pattern of biosynthetic interlock between the pathways of both amino acids in corynebacteria...
  22. pmc Transcriptional analysis and regulatory signals of the hom-thrB cluster of Brevibacterium lactofermentum
    L M Mateos
    Department of Ecology, Genetics and Microbiology, University of Leon, Spain
    J Bacteriol 176:7362-71. 1994
    ..The thrB promoter was located in an 87-bp fragment that overlaps with the end of the hom gene. A functional transcriptional terminator located downstream from the cluster was subcloned in terminator-probe vectors...
  23. ncbi request reprint Structure of the ask-asd operon and formation of aspartokinase subunits in the cephamycin producer 'Amycolatopsis lactamdurans'
    V Hernando-Rico
    Area de Microbiologia, Facultad de Ciencias Biologicas y Ambientales, Universidad de Leon, 24071 Leon, Spain
    Microbiology 147:1547-55. 2001
    ..lactamdurans' is regulated by the concerted action of lysine plus threonine and this inhibition is abolished in E. coli transformants containing Ser(301) to Tyr, or Gly(345) to Asp mutations of the 'A. lactamdurans' ask gene...
  24. ncbi request reprint Use of a cloned gene involved in candicidin production to discover new polyene producer Streptomyces strains
    J A Gil
    Area de Microbiologia, Facultad de Biologia, Universidad de Leon, Spain
    FEMS Microbiol Lett 58:15-8. 1990
    ..The pab gene hybridizes with 6 out of 16 Streptomyces strains, and those strains which hybridize turned out to be polyene producers. Such strains were never before described as polyene producers...
  25. ncbi request reprint The argG gene of Streptomyces clavuligerus has low homology to unstable argG from other actinomycetes: effect of amplification on clavulanic acid biosynthesis
    A Rodriguez-Garcia
    Faculty of Biology, University of Leon, Spain
    Gene 167:9-15. 1995
    ..Amplification of argG in transformant Scl[pULAR113] results in a 2.3-fold increase in the production of clavulanic acid (CA) in relation to the control strain Scl[pIJ699]...
  26. pmc Conversion of pipecolic acid into lysine in Penicillium chrysogenum requires pipecolate oxidase and saccharopine reductase: characterization of the lys7 gene encoding saccharopine reductase
    L Naranjo
    Area of Microbiology, Faculty of Biology and Environmental Sciences, University of Leon, Leon, Spain
    J Bacteriol 183:7165-72. 2001
    ....
  27. pmc Multiple sigma factor genes in Brevibacterium lactofermentum: characterization of sigA and sigB
    J A Oguiza
    Area of Microbiology, Faculty of Biology, University of Leon, Spain
    J Bacteriol 178:550-3. 1996
    ..sigB is closely linked to the dtxR gene (encoding a repressor of iron-regulated promoters homologous to the diphtheria toxin repressor from Corynebacterium diphtheriae...
  28. ncbi request reprint Molecular cloning of the hom-thrC-thrB cluster from Bacillus sp. ULM1: expression of the thrC gene in Escherichia coli and corynebacteria, and evolutionary relationships of the threonine genes
    M Malumbres
    Department of Ecology, Genetics and Microbiology, Faculty of Biology, University of Leon, Spain
    Folia Microbiol (Praha) 40:595-606. 1995
    ..Amino acid comparison of nine threonine synthases revealed evolutionary relationships between different groups of bacteria...
  29. ncbi request reprint Molecular control mechanisms of lysine and threonine biosynthesis in amino acid-producing corynebacteria: redirecting carbon flow
    M Malumbres
    Faculty of Biology, University of Leon, Spain
    FEMS Microbiol Lett 143:103-14. 1996
    ..Removal of bottlenecks has been achieved by amplification of genes which encode feedback resistant aspartokinase and homoserine dehydrogenase (obtained by in vitro directed mutagenesis)...
  30. ncbi request reprint A moderate amplification of the mecB gene encoding cystathionine-gamma-lyase stimulates cephalosporin biosynthesis in Acremonium chrysogenum
    K Kosalkova
    Area de Microbiologia, Facultad de Ciencias Biologicas y Ambientales, Universidad de Leon, Leon 24071, Spain
    J Ind Microbiol Biotechnol 27:252-8. 2001
    ..In conclusion, moderately increased levels of cystathionine-gamma-lyase stimulated cephalosporin production but very high levels of this enzyme were deleterious for growth and cephalosporin biosynthesis...
  31. ncbi request reprint Expression of cefD2 and the conversion of isopenicillin N into penicillin N by the two-component epimerase system are rate-limiting steps in cephalosporin biosynthesis
    R V Ullán
    Institute of Biotechnology INBIOTEC, Avda del Real N o 1, 24006 Leon, Spain
    Mol Genet Genomics 272:562-70. 2004
    ....
  32. ncbi request reprint Cloning, characterization of the acyl-CoA:6-amino penicillanic acid acyltransferase gene of Aspergillus nidulans and linkage to the isopenicillin N synthase gene
    E Montenegro
    Department of Ecology, Genetics and Microbiology, University of Leon, Spain
    Mol Gen Genet 221:322-30. 1990
    ..The penDE gene of A. nidulans is linked to the pcbC gene, which encodes the isopenicillin N synthase, as also occurs in P. chrysogenum. Both genes show the same orientation and are separated by an intergenic region of 822 nucleotides...
  33. ncbi request reprint Phosphate control of pabS gene transcription during candicidin biosynthesis
    J A Asturias
    Departamento de Ecologia, Facultad de Biologia, Universidad de Leon, Spain
    Gene 93:79-84. 1990
    ..Pi-deregulated mutants, described previously, were impaired in the transcriptional control exerted by Pi. It is concluded that Pi control of PABA synthase and Cd biosynthesis is exerted by repression of formation of the pabS mRNA...
  34. ncbi request reprint The cefT gene of Acremonium chrysogenum C10 encodes a putative multidrug efflux pump protein that significantly increases cephalosporin C production
    R V Ullán
    Area of Microbiology, Faculty of Biology and Environmental Sciences, University of Leon, 24071 Leon, Spain
    Mol Genet Genomics 267:673-83. 2002
    ..It seems that the CefT protein is involved in cephalosporin export from A. chrysogenum or in transmembrane signal transduction, and that there are redundant systems involved in cephalosporin export...
  35. ncbi request reprint Intrachromosomal recombination after targeted monocopy integration in Penicillium chrysogenum: stabilization of the direct repeats to prevent loss of the inserted gene
    O Banuelos
    Area of Microbiology, Faculty of Biology and Environmental Science, University of Leon, Spain
    Curr Genet 39:231-6. 2001
    ..Genetic stabilization of a monocopy system was obtained when both pyrG repeats (formed at the targeted integration site) contained at least one identical mutation, since in this case further recombinations can be easily counter-selected...
  36. ncbi request reprint The galE gene encoding the UDP-galactose 4-epimerase of Brevibacterium lactofermentum is coupled transcriptionally to the dmdR gene
    J A Oguiza
    Department of Ecology, Genetics and Microbiology, Faculty of Biology, University of Leon, Spain
    Gene 177:103-7. 1996
    ..In addition, the dmdR gene was also expressed as a monocistronic 0.7-kb transcript during the active growth phase...
  37. ncbi request reprint Expression of a synthetic copy of the bovine chymosin gene in Aspergillus awamori from constitutive and pH-regulated promoters and secretion using two different pre-pro sequences
    R E Cardoza
    Institute of Biotechnology of León INBIOTEC, Science Park of León, Leon, Spain
    Biotechnol Bioeng 83:249-59. 2003
    ..A very high expression from the pepB promoter was observed during the growth phase. The A. awamori pepB gene terminator was more favorable for chymosin production than the S. cerevisiae CYC1 terminator...
  38. pmc Two proteins with ornithine acetyltransferase activity show different functions in Streptomyces clavuligerus: Oat2 modulates clavulanic acid biosynthesis in response to arginine
    A de la Fuente
    Area de Microbiologia, Facultad de Ciencias Biologicas y Ambientales, Universidad de Leon, Leon, Spain
    J Bacteriol 186:6501-7. 2004
    ..Our results suggest that the Oat2 protein or one of its reaction products has a regulatory role that modulates clavulanic acid biosynthesis in response to high arginine concentrations...
  39. ncbi request reprint Construction of L-lysine-overproducing strains of Brevibacterium lactofermentum by targeted disruption of the hom and thrB genes
    C Fernandez-Gonzalez
    Departamento de Ecologia, Genética y Microbiología, Facultad de Biologia, Universidad de Leon, Spain
    Appl Microbiol Biotechnol 46:554-8. 1996
    ..B. lactofermentum R31 mutants do not overproduce lysine because R31 is an alanine-overproducing strain and channels the pyruvate needed for lysine biosynthesis to the production of alanine...
  40. pmc The two-component PhoR-PhoP system controls both primary metabolism and secondary metabolite biosynthesis in Streptomyces lividans
    A Sola-Landa
    Instituto de Biotecnología de León INBIOTEC, Parque Cientifico de Leon, Avenida del Real, n degrees 1, Spain
    Proc Natl Acad Sci U S A 100:6133-8. 2003
    ..These results prove that expression of both phoA and genes implicated in the biosynthesis of secondary metabolites in S. lividans is regulated by a mechanism involving the two-component PhoR-PhoP system...
  41. ncbi request reprint Arginine boxes and the argR gene in Streptomyces clavuligerus: evidence for a clear regulation of the arginine pathway
    A Rodriguez-Garcia
    Area of Microbiology, Faculty of Biology, University of Leon, Spain
    Mol Microbiol 25:219-28. 1997
    ..This is the first example of a clear regulation of an amino acid biosynthetic pathway in Streptomyces species, challenging the belief that actinomycetes do not have a well-developed regulatory system of these pathways...
  42. pmc Analysis and expression of the thrC gene of Brevibacterium lactofermentum and characterization of the encoded threonine synthase
    M Malumbres
    Department of Ecology, Genetics and Microbiology, Faculty of Biology, University of Leon, Spain
    Appl Environ Microbiol 60:2209-19. 1994
    ..8-kDa protein, as shown by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The purified enzyme required pyridoxal phosphate as its only cofactor to convert homoserine phosphate into threonine...
  43. ncbi request reprint Phosphate control sequences involved in transcriptional regulation of antibiotic biosynthesis
    P Liras
    Department of Ecology, Genetics and Microbiology, University of Leon, Spain
    Trends Biotechnol 8:184-9. 1990
    ..From computer analysis of sequence data, PC sequences appear to be associated with promoter regions of several phosphate-controlled antibiotic biosynthetic genes...
  44. pmc A two-protein component 7 alpha-cephem-methoxylase encoded by two genes of the cephamycin C cluster converts cephalosporin C to 7-methoxycephalosporin C
    J J Coque
    Department of Ecology, Genetics and Microbiology, Faculty of Biology, University of Leon, Spain
    J Bacteriol 177:2230-5. 1995
    ..Protein P8 may act as a coupling protein for efficient hydroxylation at C-7 in a form similar to that of the two-component system of Pseudomonas putida p-hydroxyphenylacetate-3-hydroxylase...
  45. ncbi request reprint Biosynthesis and phosphate control of candicidin by Streptomyces acrimycini JI2236: effect of amplification of the pabAB gene
    J A Asturias
    Faculty of Biology, University of Leon, Spain
    J Ind Microbiol 13:183-9. 1994
    ..Formation of PABA synthase and candicidin production was equally sensitive to phosphate regulation in transformants with the pabAB than in the untransformed S. acrimycini strain...
  46. pmc Organization and transcriptional analysis of a six-gene cluster around the rplK-rplA operon of Corynebacterium glutamicum encoding the ribosomal proteins L11 and L1
    C Barreiro
    Instituto de Biotecnologia INBIOTEC, Parque Cientifico de Leon, Avda del Real, No 1, 24006 Leon, Spain
    Appl Environ Microbiol 67:2183-90. 2001
    ..The promoters of the nusG and rplK-rplA genes were subcloned in a novel corynebacterial promoter-probe vector and shown to confer strong expression of the reporter gene...
  47. ncbi request reprint Evolution of the clusters of genes for beta-lactam antibiotics: a model for evolutive combinatorial assembly of new beta-lactams
    P Liras
    Area of Microbiology, Faculty of Biology, University of Leon, Spain
    Int Microbiol 1:271-8. 1998
    ..Comparison of proteins reveals much larger differences for primary metabolism enzymes than for beta-lactam biosynthesis enzymes in producing organisms. This suggests a horizontal transfer of the beta-lactam antibiotic biosynthesis genes...
  48. ncbi request reprint Effect of exogenous lysine on the expression of early cephamycin C biosynthetic genes and antibiotic production in Nocardia lactamdurans MA4213
    A L Leitão
    Area of Microbiology, Faculty of Biology, University of Leon, Spain
    Appl Microbiol Biotechnol 56:670-5. 2001
    ..These results indicate that L-lysine is required not only for antibiotic biosynthesis, but particularly as carbon or nitrogen source...
  49. ncbi request reprint The pab gene of Streptomyces griseus, encoding p-aminobenzoic acid synthase, is located between genes possibly involved in candicidin biosynthesis
    L M Criado
    Departamento de Ecologia, Genética y Microbiología, Universidad de Leon, Spain
    Gene 126:135-9. 1993
    ..The deletion occurred when the gene was cloned in the BamHI site of pBR322 and allowed pab expression in E. coli...
  50. ncbi request reprint Nitrate regulation of alpha-aminoadipate reductase formation and lysine inhibition of its activity in Penicillium chrysogenum and Acremonium chrysogenum
    M J Hijarrubia
    Instituto de Biotecnologia de Leon, INBIOTEC, Parque Cientifico de Leon, Avda del Real, No 1, Spain
    Appl Microbiol Biotechnol 59:270-7. 2002
    ..Nitrate has a strong negative effect on alpha-AAR formation as shown by immunoblotting studies of alpha-AAR. The nitrate effect was reversed by lysine...
  51. ncbi request reprint Agrobacterium tumefaciens-mediated transformation of the antitumor clavaric acid-producing basidiomycete Hypholoma sublateritium
    R P Godio
    Institute of Biotechnology of León, INBIOTEC, Science Park, Av Real 1, 24006 Leon, Spain
    Curr Genet 46:287-94. 2004
    ..bisporus gpd promoter, as shown by RT-PCR studies, but no significant fluorescence was observed. Transformation of H. sublateritium opens the way for the genetic manipulation of clavaric acid biosynthesis in this fungus...
  52. doi request reprint Transcriptional analysis and proteomics of the holomycin gene cluster in overproducer mutants of Streptomyces clavuligerus
    V Robles-Reglero
    Area de Microbiologia, Facultad de Ciencias Biologicas y Ambientales, Universidad de Leon, 24071 Leon, Spain
    J Biotechnol 163:69-76. 2013
    ..coelicolor M1154 strain, suggesting that heterologous expression in S. coelicolor might be toxic due to the lack of an holomycin resistance gene in this host strain...
  53. doi request reprint Characterization of DNA-binding sequences for CcaR in the cephamycin-clavulanic acid supercluster of Streptomyces clavuligerus
    I Santamarta
    INBIOTEC, Instituto de Biotecnologia de Leon, Avda Real n 1, Parque Cientifico de Leon, 24006 Leon, Spain
    Mol Microbiol 81:968-81. 2011
    ..Footprinting experiments indicated that triple heptameric conserved sequences were protected by r-CcaR, and allowed identification of heptameric sequences as CcaR binding sites...
  54. ncbi request reprint A complex multienzyme system encoded by five polyketide synthase genes is involved in the biosynthesis of the 26-membered polyene macrolide pimaricin in Streptomyces natalensis
    J F Aparicio
    Institute of Biotechnology INBIOTEC, Parque Cientifico de Leon, Leon, France
    Chem Biol 7:895-905. 2000
    ..We set out to clone, sequence and analyse the gene cluster responsible for the biosynthesis of this tetraene...
  55. pmc Gene targeting in Penicillium chrysogenum: disruption of the lys2 gene leads to penicillin overproduction
    J Casqueiro
    Institute of Biotechnology INBIOTEC, 24006 Leon, Spain
    J Bacteriol 181:1181-8. 1999
    ..The alpha-aminoadipic acid precursor is channelled to penicillin biosynthesis by blocking the lysine biosynthesis branch at the alpha-aminoadipate reductase level...
  56. doi request reprint CefR modulates transporters of beta-lactam intermediates preventing the loss of penicillins to the broth and increases cephalosporin production in Acremonium chrysogenum
    F Teijeira
    Institute of Biotechnology, Av Real s n, Leon, Spain
    Metab Eng 13:532-43. 2011
    ..In summary, we describe for the first time a modulator of beta-lactam intermediate transporters in A. chrysogenum...
  57. ncbi request reprint Lysine is catabolized to 2-aminoadipic acid in Penicillium chrysogenum by an omega-aminotransferase and to saccharopine by a lysine 2-ketoglutarate reductase. Characterization of the omega-aminotransferase
    E M Martín de Valmaseda
    Area de Microbiologia, Fac CC Biológicas y Ambientales, Universidad de Leon, Campus de Vegazana, S N, Spain
    Mol Genet Genomics 274:272-82. 2005
    ..This information was used to clone the gene encoding this enzyme...
  58. pmc Molecular cloning, DNA sequence analysis, and characterization of the Corynebacterium diphtheriae dtxR homolog from Brevibacterium lactofermentum
    J A Oguiza
    Area of Microbiology, Faculty of Biology, University of Leon, Spain
    J Bacteriol 177:465-7. 1995
    ..The extents of induction by increasing concentrations of the chelator 2,2'-dipyridyl were identical in cells expressing DtxR from either C. diphtheriae or B. lactofermentum...
  59. ncbi request reprint High transformation frequency of nonsporulating mutants of Streptomyces griseus
    A Daza
    Departamento de Ecologia, Facultad de Biologia, Universidad de Leon, Spain
    FEMS Microbiol Lett 59:259-64. 1990
    ..Antibiotic and pigment production, and low transformability with plasmid DNA were together restored in spontaneous spo+ revertants...
  60. ncbi request reprint Genes directly involved in the biosynthesis of beta-lactam antibiotics
    J L Barredo
    Departamento de Ecologia, Genética y Microbiología, Universidad de Leon, Spain
    Microbiologia 7:1-12. 1991
    ..The enzymes which catalyze these last three steps are encoded by the genes cefE, cefF and cefG. The penicillin, cephalosporin and cephamycin biosynthetic genes are organized in clusters (and subclusters) of genes...
  61. pmc Nucleotide sequence of the homoserine dehydrogenase (thr A) gene of Brevibacterium lactofermentum
    L M Mateos
    Area de Microbiologia, Facultad de Biologia, Universidad de Leon, Spain
    Nucleic Acids Res 15:10598. 1987
  62. pmc Characterization of the Streptomyces clavuligerus argC gene encoding N-acetylglutamyl-phosphate reductase: expression in Streptomyces lividans and effect on clavulanic acid production
    M Ludovice
    Area of Microbiology, Faculty of Biology, University of Leon, Spain
    J Bacteriol 174:4606-13. 1992
    ..Transformation of S. clavuligerus 328, an argC auxotroph deficient in clavulanic acid biosynthesis, with plasmid pULML30, carrying the cloned argC gene, restored both prototrophy and antibiotic production...
  63. ncbi request reprint Polyene macrolide antibiotic biosynthesis
    J F Aparicio
    Institute of Biotechnology INBIOTEC, Parque Cientifico de Leon, and Area of Microbiology, Faculty of Biology, University of Leon, Leon, Spain
    Curr Med Chem 11:1645-56. 2004
    ....
  64. doi request reprint Proteolytic activity, mycotoxins and andrastin A in Penicillium roqueforti strains isolated from Cabrales, Valdeón and Bejes-Tresviso local varieties of blue-veined cheeses
    M A Fernández-Bodega
    Institute of Biotechnology of León INBIOTEC, Leon, Spain
    Int J Food Microbiol 136:18-25. 2009
    ..Andrastin A was present in all industrial and local varieties of blue cheese. The content of andrastin A was similar to that of other well-known blue cheeses from France and Denmark...
  65. ncbi request reprint Expression of the recombinant protein disulphide isomerase of Teladorsagia circumcincta
    M Martinez-Valladares
    Departamento de Sanidad Animal, Parasitologia, Facultad de Veterinaria, Universidad de Leon, Leon, Spain
    Parasite Immunol 29:47-56. 2007
    ..Among all proteins, three showed significant associations between protein recognition and the titre of IgA in the serum...