V Sanchez-Margalet

Summary

Affiliation: Instituto de la Grasa
Country: Spain

Publications

  1. ncbi Sam68 is a docking protein linking GAP and PI3K in insulin receptor signaling
    V Sanchez-Margalet
    Department of Medical Biochemistry and Molecular Biology, School of Medicine, University of Seville and Investigation Unit, University Hospital Virgen Macarena, Av Sanchez Pizjuan 4, 41009 Seville, Spain
    Mol Cell Endocrinol 183:113-21. 2001
  2. ncbi Pancreastatin, a chromogranin A-derived peptide, inhibits DNA and protein synthesis by producing nitric oxide in HTC rat hepatoma cells
    V Sanchez-Margalet
    Department of Medical Biochemistry and Molecular Biology, School of Medicine, University Hospital Virgen Macarena, Seville, Spain
    J Hepatol 35:80-5. 2001
  3. ncbi Characterization of pancreastatin receptor and signaling in rat HTC hepatoma cells
    V Sanchez-Margalet
    Departamento de Bioquimica Medica y Biologia Molecular, Facultad de Medicina, Unidad de Investigacion, Hospital Universitario Virgen Macarena, Universidad de Sevilla, Av Sanchez Pizjuan 4, 41009, Sevilla, Spain
    Eur J Pharmacol 397:229-35. 2000
  4. ncbi Stimulation of glycogen synthesis by insulin requires S6 kinase and phosphatidylinositol-3-kinase in HTC-IR cells
    V Sanchez-Margalet
    Departamento de Bioquimica Medica y Biologia Molecular, Facultad de Medicina, Universidad de Sevilla Unidad de Investigación del Hospital Universitario Virgen Macarena, Sevilla, Spain
    J Cell Physiol 182:182-8. 2000
  5. ncbi p68 Sam is a substrate of the insulin receptor and associates with the SH2 domains of p85 PI3K
    V Sanchez-Margalet
    Department of Medical Biochemistry and Molecular Biology, School of Medicine, University of Seville, University Hospital Virgen Macarena, Spain
    FEBS Lett 455:307-10. 1999
  6. ncbi Solubilization and molecular characterization of active pancreastatin receptors from rat liver membranes
    V Sanchez-Margalet
    Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, University of Seville, Spain
    Endocrinology 138:1712-8. 1997
  7. ncbi Human leptin signaling in human peripheral blood mononuclear cells: activation of the JAK-STAT pathway
    V Sanchez-Margalet
    Department of Medical Biochemistry and Molecular Biology, School of Medicine, Investigation Unit, University of Seville, 41009 Seville, Spain
    Cell Immunol 211:30-6. 2001
  8. pmc Sam68 is tyrosine phosphorylated and recruited to signalling in peripheral blood mononuclear cells from HIV infected patients
    S Najib
    Department of Clinical Biochemistry, Virgen Macarena University Hospital, Seville, Spain
    Clin Exp Immunol 141:518-25. 2005
  9. pmc Role of leptin as an immunomodulator of blood mononuclear cells: mechanisms of action
    V Sanchez-Margalet
    Department of Clinical Biochemistry, Investigation Unit, University Hospital Virgen Macarena, Seville, Spain
    Clin Exp Immunol 133:11-9. 2003
  10. pmc Leptin receptor (Ob-R) expression is induced in peripheral blood mononuclear cells by in vitro activation and in vivo in HIV-infected patients
    V Sanchez-Margalet
    Department of Clinical Biochemistry, Internal Medicine, Virgen Macarena University Hospital, Seville, Spain
    Clin Exp Immunol 129:119-24. 2002

Collaborators

  • R Goberna
  • C K Sung
  • C Gonzalez-Yanes
  • A Perez-Perez
  • S Najib
  • F Sanchez-Jimenez
  • J Martín-González
  • C L Varone
  • R Quintana-Portillo
  • C Martin-Romero
  • P Fernández-Riejos
  • M A Muniain
  • J Rodríguez-Baño
  • C Sánchez-Pozo
  • P Guadix
  • J L Maymó
  • J J Segura-Egea
  • A Carmona-Fernández
  • J L Dueñas
  • Y P Gambino
  • A Canfrán-Duque
  • T Issad
  • M J Rios
  • A Domínguez-Castellano
  • J Santos-Alvarez

Detail Information

Publications26

  1. ncbi Sam68 is a docking protein linking GAP and PI3K in insulin receptor signaling
    V Sanchez-Margalet
    Department of Medical Biochemistry and Molecular Biology, School of Medicine, University of Seville and Investigation Unit, University Hospital Virgen Macarena, Av Sanchez Pizjuan 4, 41009 Seville, Spain
    Mol Cell Endocrinol 183:113-21. 2001
    ..e. p120GAP. In this way, these signaling complexes may modulate other signaling cascades of IR, such as p21Ras pathway...
  2. ncbi Pancreastatin, a chromogranin A-derived peptide, inhibits DNA and protein synthesis by producing nitric oxide in HTC rat hepatoma cells
    V Sanchez-Margalet
    Department of Medical Biochemistry and Molecular Biology, School of Medicine, University Hospital Virgen Macarena, Seville, Spain
    J Hepatol 35:80-5. 2001
    ..These data suggested that pancreastatin might have a role in growth and proliferation, similar to other calcium-mobilizing hormones...
  3. ncbi Characterization of pancreastatin receptor and signaling in rat HTC hepatoma cells
    V Sanchez-Margalet
    Departamento de Bioquimica Medica y Biologia Molecular, Facultad de Medicina, Unidad de Investigacion, Hospital Universitario Virgen Macarena, Universidad de Sevilla, Av Sanchez Pizjuan 4, 41009, Sevilla, Spain
    Eur J Pharmacol 397:229-35. 2000
    ..We found that pancreastatin receptor was coupled with a G alpha(q/11) protein which activates phospholipase C-beta(1) and phospholipase C-beta(3), in addition to MAPK via both beta gamma and alpha(q/11)...
  4. ncbi Stimulation of glycogen synthesis by insulin requires S6 kinase and phosphatidylinositol-3-kinase in HTC-IR cells
    V Sanchez-Margalet
    Departamento de Bioquimica Medica y Biologia Molecular, Facultad de Medicina, Universidad de Sevilla Unidad de Investigación del Hospital Universitario Virgen Macarena, Sevilla, Spain
    J Cell Physiol 182:182-8. 2000
    ....
  5. ncbi p68 Sam is a substrate of the insulin receptor and associates with the SH2 domains of p85 PI3K
    V Sanchez-Margalet
    Department of Medical Biochemistry and Molecular Biology, School of Medicine, University of Seville, University Hospital Virgen Macarena, Spain
    FEBS Lett 455:307-10. 1999
    ..This association may be important for the link of the signaling with RNA metabolism...
  6. ncbi Solubilization and molecular characterization of active pancreastatin receptors from rat liver membranes
    V Sanchez-Margalet
    Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, University of Seville, Spain
    Endocrinology 138:1712-8. 1997
    ..We conclude that we solubilized active pancreastatin receptors from rat liver membranes, and these results support the conclusion that the liver pancreastatin receptor consists of a 80,000 Mr glycoprotein associated with G proteins...
  7. ncbi Human leptin signaling in human peripheral blood mononuclear cells: activation of the JAK-STAT pathway
    V Sanchez-Margalet
    Department of Medical Biochemistry and Molecular Biology, School of Medicine, Investigation Unit, University of Seville, 41009 Seville, Spain
    Cell Immunol 211:30-6. 2001
    ..This pathway may mediate, at least in part, the action of human leptin in human peripheral blood mononuclear cells...
  8. pmc Sam68 is tyrosine phosphorylated and recruited to signalling in peripheral blood mononuclear cells from HIV infected patients
    S Najib
    Department of Clinical Biochemistry, Virgen Macarena University Hospital, Seville, Spain
    Clin Exp Immunol 141:518-25. 2005
    ..In contrast, PBMC from HIV infected subjects have lower expression levels of Sam68 compared with controls. These results suggest that Sam68 may play some role in the immune function of lymphocytes in HIV infection...
  9. pmc Role of leptin as an immunomodulator of blood mononuclear cells: mechanisms of action
    V Sanchez-Margalet
    Department of Clinical Biochemistry, Investigation Unit, University Hospital Virgen Macarena, Seville, Spain
    Clin Exp Immunol 133:11-9. 2003
    ..Moreover, these immunoregulatory functions of leptin could have some relevance in the pathophysiology of obesity...
  10. pmc Leptin receptor (Ob-R) expression is induced in peripheral blood mononuclear cells by in vitro activation and in vivo in HIV-infected patients
    V Sanchez-Margalet
    Department of Clinical Biochemistry, Internal Medicine, Virgen Macarena University Hospital, Seville, Spain
    Clin Exp Immunol 129:119-24. 2002
    ..These results are consistent with the suggested role of leptin in modulating the immune response...
  11. ncbi Human leptin activates PI3K and MAPK pathways in human peripheral blood mononuclear cells: possible role of Sam68
    C Martin-Romero
    Department of Clinical Biochemistry, Virgen Macarena University Hospital, University of Seville, 41071 Seville, Spain
    Cell Immunol 212:83-91. 2001
    ..These signal transduction pathways may mediate the described effects of human leptin on human peripheral blood mononuclear cells...
  12. pmc Leptin promotes cell survival and activates Jurkat T lymphocytes by stimulation of mitogen-activated protein kinase
    P Fernández-Riejos
    Department of Clinical Biochemistry, Virgen Macarena University Hospital, Medical School University of Seville, Seville, Spain
    Clin Exp Immunol 151:505-18. 2008
    ....
  13. ncbi Role of Sam68 as an adaptor protein in signal transduction
    S Najib
    Department of Medical Biochemistry and Molecular Biology, School of Medicine, Investigation Unit, Virgen Macarena University Hospital, Av Sanchez Pizjuan 4, Seville 41009, Spain
    Cell Mol Life Sci 62:36-43. 2005
    ..Finally, the connection between this role of Sam68 in protein-protein interaction with RNA binding activity may connect signal transduction of tyrosine kinases with the regulation of RNA metabolism...
  14. ncbi The expression of Sam68, a protein involved in insulin signal transduction, is enhanced by insulin stimulation
    V Sanchez-Margalet
    Department of Medical Biochemistry and Molecular Biology, Medical School, Investigation Unit, Virgen Macarena University Hospital, Av Sanchez Pizjuan 4, Seville 41009, Spain
    Cell Mol Life Sci 60:751-8. 2003
    ....
  15. ncbi Pancreastatin, a chromogranin-A-derived peptide, inhibits insulin-stimulated glycogen synthesis by activating GSK-3 in rat adipocytes
    C Gonzalez-Yanes
    Department of Medical Biochemistry and Molecular Biology, Investigation Unit University Hospital Virgen Macarena, School of Medicine, University of Seville, Spain
    Biochem Biophys Res Commun 289:282-7. 2001
    ..This effect of PST can also be prevented by using a PKC inhibitor. In conclusion, the chromogranin-A-derived peptide PST inhibits glycogen synthesis in rat adipocytes by activating GSK-3 activity through the activation of PKC...
  16. ncbi Pancreastatin, a chromogranin A-derived peptide, inhibits leptin and enhances UCP-2 expression in isolated rat adipocytes
    C Gonzalez-Yanes
    Department of Medical Biochemistry and Molecular Biology, School of Medicine, Investigation Unit, Virgen Macarena University Hospital, Av Sanchez Pizjuan 4, Seville 41009, Spain
    Cell Mol Life Sci 60:2749-56. 2003
    ..These effects may contribute to the metabolic action of catecholamines in physiological and pathophysiological conditions with increased sympathetic activity...
  17. ncbi Pancreastatin, a chromogranin A-derived peptide, activates Galpha(16) and phospholipase C-beta(2) by interacting with specific receptors in rat heart membranes
    C Gonzalez-Yanes
    Department of Medical Biochemistry and Molecular Biology, School of Medicine, University Hospital Virgen Macarena, Av Sanchez Pizjuan 4, 41009, Seville, Spain
    Cell Signal 13:43-9. 2001
    ..We have found that PST stimulates PLCbeta(2)>PLCbeta(1)>PLCbeta(3) by activating Galpha(16) in rat heart membranes. These data suggest that PST may modulate the cardiac function...
  18. pmc Leptin stimulates the oxidative burst in control monocytes but attenuates the oxidative burst in monocytes from HIV-infected patients
    C Sánchez-Pozo
    Department of Clinical Biochemistry, Virgen Macarena University Hospital, Seville, Spain
    Clin Exp Immunol 134:464-9. 2003
    ..On the other hand, the inhibitory effect on monocytes from HIV+ subjects may be explained by the attenuation of the oxidative burst by a delayed activation of monocytes in a hyperinflammatory state...
  19. ncbi Homocysteine thiolactone inhibits insulin-stimulated DNA and protein synthesis: possible role of mitogen-activated protein kinase (MAPK), glycogen synthase kinase-3 (GSK-3) and p70 S6K phosphorylation
    S Najib
    Department of Medical Biochemistry and Molecular Biology, School of Medicine Investigation Unit, Virgen Macarena University Hospital, Av Sanchez Pizjuan 4, Seville 41009, Spain
    J Mol Endocrinol 34:119-26. 2005
    ..In conclusion, these data suggest that homocysteine thiolactone impairs insulin signaling by a mechanism involving oxidative stress, leading to a defect in the action of insulin on growth and proliferation...
  20. ncbi Increased plasma pancreastatin-like levels in gestational diabetes: correlation with catecholamine levels
    V Sanchez-Margalet
    Department of Clinical Biochemistry, University Hospital Virgen Macarena, Seville, Spain
    Diabetes Care 21:1951-4. 1998
    ..To investigate plasma pancreastatin (a chromogranin A-derived peptide) and catecholamine levels (counterregulatory hormones) in subjects with gestational diabetes compared with normal pregnant subjects...
  21. doi Activated translation signaling in placenta from pregnant women with gestational diabetes mellitus: possible role of leptin
    A Perez-Perez
    Departamento de Bioquimica Medica y Biologia Molecular, Facultad de Medicina, Hospital Universitario Virgen Macarena, Universidad de Sevilla, Spain
    Horm Metab Res 45:436-42. 2013
    ..The increased expression of leptin and LEPR may contribute to these effects. These results may provide a possible mechanism for the previously observed increase in placenta growth in GDM...
  22. doi Sam68 mediates leptin-stimulated growth by modulating leptin receptor signaling in human trophoblastic JEG-3 cells
    F Sanchez-Jimenez
    Department of Clinical Biochemistry, Virgen Macarena University Hospital, University of Seville, Seville 41071, Spain
    Hum Reprod 26:2306-15. 2011
    ..In the present work, we have studied the participation of Sam68 in the main signaling pathways activated by LEPR to increase growth and proliferation in trophoblastic JEG-3 cells...
  23. doi Sam68 interacts with IRS1
    R Quintana-Portillo
    Department of Clinical Biochemistry, Virgen Macarena University Hospital, University of Seville, Spain
    Biochem Pharmacol 83:78-87. 2012
    ..In conclusion, our data indicate that Sam68 interacts with IRS-1 in basal conditions, and insulin increases the affinity between these two partners...
  24. doi Leptin expression in healthy and inflamed human dental pulp
    J Martín-González
    Department of Stomatology Endodontics section, School of Dentistry, University of Sevilla, Spain
    Int Endod J 46:442-8. 2013
    ..To investigate the expression of leptin in healthy and inflamed human dental pulp...
  25. ncbi Glucogenolytic and hyperglycemic effect of 33-49 C-terminal fragment of pancreastatin in the rat in vivo
    V Sanchez-Margalet
    Departamento de Bioquimica Medica y Biologia Molecular, Facultad de Medicina, Universidad de Sevilla, Spain
    Horm Metab Res 24:455-7. 1992
    ..Blood glucose levels were increased by the C-terminal fragment of pancreastatin. This study shows that the 33-49 C-terminal fragment of pancreatasin could play a role in glucose metabolism not mediated by insulin or glucagon...
  26. ncbi Guanosine triphosphatase-activating protein-associated protein, but not src-associated protein p68 in mitosis, is a part of insulin signaling complexes
    C K Sung
    Department of Physiology and Biophysics, University of Southern California School of Medicine, Los Angeles 90033, USA
    Endocrinology 139:2392-8. 1998
    ..These data suggest that 1) GAP-associated protein, but not Sam68, is a part of insulin signaling complexes; and 2) p85alpha and p85beta form similar, but distinct, insulin receptor signaling complexes...