Mariano Higes

Summary

Affiliation: Hospital Virgen de la Salud
Country: Spain

Publications

  1. ncbi request reprint Apoptosis in the pathogenesis of Nosema ceranae (Microsporidia: Nosematidae) in honey bees (Apis mellifera)
    Mariano Higes
    Laboratorio de Patología Apícola, Centro Apicola Regional, JCCM, 19180, Marchamalo, Spain
    Environ Microbiol Rep 5:530-6. 2013
  2. doi request reprint Nosema ceranae (Microsporidia), a controversial 21st century honey bee pathogen
    Mariano Higes
    Centro Apícola Regional CAR, Dirección General de la Producción Agropecuaria, Consejeria de Agricultura, Junta de Castilla La Mancha, Spain
    Environ Microbiol Rep 5:17-29. 2013
  3. ncbi request reprint Nosema ceranae, a new microsporidian parasite in honeybees in Europe
    Mariano Higes
    Centro Apicola Regional, Consejeria de Agricultura, Junta de Comunidades de Castilla La Mancha, Marchamalo, 19180 Guadalajara, Spain
    J Invertebr Pathol 92:93-5. 2006
  4. ncbi request reprint Experimental infection of Apis mellifera honeybees with Nosema ceranae (Microsporidia)
    Mariano Higes
    Regional Apicultural Center, Dirección General de la Producción Agropecuaria, Consejeria de Agricultura, Junta de Comunidades de Castilla La Mancha, Marchamalo, Guadalajara, Spain
    J Invertebr Pathol 94:211-7. 2007
  5. ncbi request reprint Detection of infective Nosema ceranae (Microsporidia) spores in corbicular pollen of forager honeybees
    Mariano Higes
    Regional Apicultural Center, Dirección General de la Producción Agropecuaria, Consejeria de Agricultura, Junta de Comunidades de Castilla La Mancha, 19180 Marchamalo, Guadalajara, Spain
    J Invertebr Pathol 97:76-8. 2008
  6. doi request reprint Regurgitated pellets of Merops apiaster as fomites of infective Nosema ceranae (Microsporidia) spores
    Mariano Higes
    Regional Apicultural Center, Dirección General de la Producción Agropecuaria, Consejeria de Agricultura, Junta de Comunidades de Castilla La Mancha, Marchamalo, Guadalajara, Spain
    Environ Microbiol 10:1374-9. 2008
  7. doi request reprint The effect of induced queen replacement on Nosema spp. infection in honey bee (Apis mellifera iberiensis) colonies
    Cristina Botías
    Laboratorio de Patología Apícola, Centro Apicola Regional, JCCM, 19180 Marchamalo, Spain
    Environ Microbiol 14:845-59. 2012
  8. doi request reprint The prevalence of Acarapis woodi in Spanish honey bee (Apis mellifera) colonies
    Encarna Garrido-Bailón
    Bee Pathology laboratory, Centro Apicola Regional, Junta de Comunidades de Castilla La Mancha, 19180 Marchamalo, Spain
    Exp Parasitol 132:530-6. 2012
  9. ncbi request reprint Screening alternative therapies to control Nosemosis type C in honey bee (Apis mellifera iberiensis) colonies
    Cristina Botías
    Laboratorio de Patología Apícola, Centro Apícola Regional CAR, Junta de Comunidades de Castilla La Mancha, 19180 Marchamalo, Spain Electronic address
    Res Vet Sci 95:1041-5. 2013
  10. doi request reprint Microsporidia infecting Apis mellifera: coexistence or competition. Is Nosema ceranae replacing Nosema apis?
    Raquel Martín-Hernández
    Bee Pathology laboratory, Centro Apicola Regional, JCCM, 19180 Marchamalo, Spain
    Environ Microbiol 14:2127-38. 2012

Collaborators

Detail Information

Publications27

  1. ncbi request reprint Apoptosis in the pathogenesis of Nosema ceranae (Microsporidia: Nosematidae) in honey bees (Apis mellifera)
    Mariano Higes
    Laboratorio de Patología Apícola, Centro Apicola Regional, JCCM, 19180, Marchamalo, Spain
    Environ Microbiol Rep 5:530-6. 2013
    ..Our results suggested that N. ceranae prevents apoptosis in epithelial cells of infected ventriculi, a mechanism possible designed to enhance parasite development...
  2. doi request reprint Nosema ceranae (Microsporidia), a controversial 21st century honey bee pathogen
    Mariano Higes
    Centro Apícola Regional CAR, Dirección General de la Producción Agropecuaria, Consejeria de Agricultura, Junta de Castilla La Mancha, Spain
    Environ Microbiol Rep 5:17-29. 2013
    ..Not only does this type of nosemosis causes a clear pathology on honeybees at both the individual and colony levels, but it also has significant effects on the production of honeybee products...
  3. ncbi request reprint Nosema ceranae, a new microsporidian parasite in honeybees in Europe
    Mariano Higes
    Centro Apicola Regional, Consejeria de Agricultura, Junta de Comunidades de Castilla La Mancha, Marchamalo, 19180 Guadalajara, Spain
    J Invertebr Pathol 92:93-5. 2006
    ..This is the first report of N. ceranae in colonies of Apis mellifera in Europe. The suggested link of the infections to clinical disease symptoms makes imperative a study of the virulence of N. ceranae in European races of honey bees...
  4. ncbi request reprint Experimental infection of Apis mellifera honeybees with Nosema ceranae (Microsporidia)
    Mariano Higes
    Regional Apicultural Center, Dirección General de la Producción Agropecuaria, Consejeria de Agricultura, Junta de Comunidades de Castilla La Mancha, Marchamalo, Guadalajara, Spain
    J Invertebr Pathol 94:211-7. 2007
    ..ceranae is highly pathogenic to Apis mellifera. Possible relation with bee depopulation syndrome is discussed by authors...
  5. ncbi request reprint Detection of infective Nosema ceranae (Microsporidia) spores in corbicular pollen of forager honeybees
    Mariano Higes
    Regional Apicultural Center, Dirección General de la Producción Agropecuaria, Consejeria de Agricultura, Junta de Comunidades de Castilla La Mancha, 19180 Marchamalo, Guadalajara, Spain
    J Invertebr Pathol 97:76-8. 2008
    ..Spore infectivity is shown after artificial infection of Nosema-free adult bees. The epidemiological consequences of the presence of Nosema spores in corbicular pollen require more study and must be considered in beekeeping practices...
  6. doi request reprint Regurgitated pellets of Merops apiaster as fomites of infective Nosema ceranae (Microsporidia) spores
    Mariano Higes
    Regional Apicultural Center, Dirección General de la Producción Agropecuaria, Consejeria de Agricultura, Junta de Comunidades de Castilla La Mancha, Marchamalo, Guadalajara, Spain
    Environ Microbiol 10:1374-9. 2008
    ..ceranae spores still remain viable and their infectivity is shown after artificial infection of Nosema-free 8-day-old adult bees. The epidemiological consequences of the presence of Nosema spores in this fomites are discussed...
  7. doi request reprint The effect of induced queen replacement on Nosema spp. infection in honey bee (Apis mellifera iberiensis) colonies
    Cristina Botías
    Laboratorio de Patología Apícola, Centro Apicola Regional, JCCM, 19180 Marchamalo, Spain
    Environ Microbiol 14:845-59. 2012
    ..The ovaries and ventriculi of queens in infected colonies revealed no signs of Nosema infection and there were no lesions in ovarioles or epithelial ventricular cells...
  8. doi request reprint The prevalence of Acarapis woodi in Spanish honey bee (Apis mellifera) colonies
    Encarna Garrido-Bailón
    Bee Pathology laboratory, Centro Apicola Regional, Junta de Comunidades de Castilla La Mancha, 19180 Marchamalo, Spain
    Exp Parasitol 132:530-6. 2012
    ..woodi and other species (Acarapis dorsalis and Acarapis externus) at the molecular level. Furthermore, the patterns of genetic divergence across sequences raised serious doubts about the current classification of these organisms...
  9. ncbi request reprint Screening alternative therapies to control Nosemosis type C in honey bee (Apis mellifera iberiensis) colonies
    Cristina Botías
    Laboratorio de Patología Apícola, Centro Apícola Regional CAR, Junta de Comunidades de Castilla La Mancha, 19180 Marchamalo, Spain Electronic address
    Res Vet Sci 95:1041-5. 2013
    ....
  10. doi request reprint Microsporidia infecting Apis mellifera: coexistence or competition. Is Nosema ceranae replacing Nosema apis?
    Raquel Martín-Hernández
    Bee Pathology laboratory, Centro Apicola Regional, JCCM, 19180 Marchamalo, Spain
    Environ Microbiol 14:2127-38. 2012
    ..This IPC provides a useful tool for laboratory detection of honeybee pathogens...
  11. doi request reprint The growing prevalence of Nosema ceranae in honey bees in Spain, an emerging problem for the last decade
    Cristina Botías
    Centro Apícola Regional CAR, Dirección General de la Producción Agropecuaria, Consejeria de Agricultura, Junta de Comunidades de Castilla La Mancha, 19180 Marchamalo, Spain
    Res Vet Sci 93:150-5. 2012
    ..The current prevalence of nosemosis, primarily due to N. ceranae, has reached epidemic levels in Spain as confirmed by the analysis of worker honey bees and commercial honey...
  12. pmc Nosema spp. infection and its negative effects on honey bees (Apis mellifera iberiensis) at the colony level
    Cristina Botías
    Laboratorio de Patología Apícola, Centro Apicola Regional, CAR, Junta de Comunidades de Castilla La Mancha, Marchamalo 19180, Spain
    Vet Res 44:25. 2013
    ..Further research is necessary to identify possible treatments or beekeeping techniques that will limit the rapid spread of this dangerous emerging disease...
  13. pmc Outcome of colonization of Apis mellifera by Nosema ceranae
    Raquel Martín-Hernández
    Regional Apicultural Center, Junta de Comunidades de Castilla La Mancha, 19180 Marchamalo, Spain
    Appl Environ Microbiol 73:6331-8. 2007
    ..A significant causative association between the presence of N. ceranae and hive depopulation clearly indicates that the colonization of Apis mellifera by N. ceranae is related to bee losses...
  14. pmc Effect of temperature on the biotic potential of honeybee microsporidia
    Raquel Martín-Hernández
    Centro Apicola Regional, Bee Pathology laboratory, Dirección General de Desarrollo Rural, Consejeria de Agricultura, Junta de Comunidades de Castilla La Mancha, 19180 Marchamalo, Spain
    Appl Environ Microbiol 75:2554-7. 2009
    ..ceranae was higher than that of N. apis. The better adaptation of N. ceranae to complete its endogenous cycle at different temperatures clearly supports the observation of the different epidemiological patterns...
  15. pmc The prevalence of the honeybee brood pathogens Ascosphaera apis, Paenibacillus larvae and Melissococcus plutonius in Spanish apiaries determined with a new multiplex PCR assay
    Encarna Garrido-Bailón
    Bee Pathology laboratory, Centro Apícola Regional CAR, Junta de Comunidades de Castilla La Mancha, 19180, Marchamalo, Spain
    Microb Biotechnol 6:731-9. 2013
    ..This technique was then used to evaluate the prevalence of these pathogens in Spanish honeybee colonies in 2006 and 2007, revealing our results a low prevalence of these pathogens in most of the geographic areas studied. ..
  16. ncbi request reprint Further evidence of an oriental origin for Nosema ceranae (Microsporidia: Nosematidae)
    Cristina Botías
    Laboratorio de Patología Apícola, Centro Apicola Regional, CAR, Dirección General de la Producción Agropecuaria, Consejeria de Agricultura, Junta de Comunidades de Castilla La Mancha, 19180 Marchamalo, Spain
    J Invertebr Pathol 110:108-13. 2012
    ..koschevnikovi from Borneo. Our findings provide further support for the hypothesis that N. ceranae has only recently emerged from Asia to become a parasite of A. mellifera...
  17. doi request reprint How natural infection by Nosema ceranae causes honeybee colony collapse
    Mariano Higes
    Bee Pathology laboratory, Centro Apicola Regional, JCCM, 19180 Marchamalo, Spain
    Environ Microbiol 10:2659-69. 2008
    ....
  18. doi request reprint Nosema spp. parasitization decreases the effectiveness of acaricide strips (Apivar(®) ) in treating varroosis of honey bee (Apis mellifera iberiensis) colonies
    Cristina Botías
    Laboratorio de Patología Apícola, Centro Apicola Regional, CAR, Junta de Comunidades de Castilla La Mancha, 19180 Marchamalo, Spain
    Environ Microbiol Rep 4:57-65. 2012
    ..These results should be considered when assessing acaricide treatments in field conditions due to the high prevalence of both pathogens worldwide...
  19. doi request reprint Honeybee colony collapse due to Nosema ceranae in professional apiaries
    Mariano Higes
    Bee Pathology Laboratory and Hive Products Laboratory, Centro Apicola Regional, JCCM, 19180 Marchamalo, Spain Animal Medicine and Surgery Department and Animal Health Department, Veterinary School, Universidad Complutense de Madrid, 28040 Madrid, Spain Analitical Chemistry Department, Sciences School, Universidad de Valladolid, 47005 Valladolid, Spain
    Environ Microbiol Rep 1:110-3. 2009
    ..The treatment with fumagillin avoided the loss of surviving weak colonies. This is the first case report of honeybee colony collapse due to N. ceranae in professional apiaries in field conditions reported worldwide...
  20. doi request reprint Comparison of the energetic stress associated with experimental Nosema ceranae and Nosema apis infection of honeybees (Apis mellifera)
    Raquel Martín-Hernández
    Bee Pathology laboratory, Centro Apicola Regional, JCCM, San Martín s n, 19180 Marchamalo, Guadalajara, Spain
    Parasitol Res 109:605-12. 2011
    ..The repercussions of this increased energetic stress may even explain the changes in host behavior due to starvation, lack of thermoregulatory capacity, or higher rates of trophallaxis, which might enhance transmission and bee death...
  21. doi request reprint Critical aspects of the Nosema spp. diagnostic sampling in honey bee (Apis mellifera L.) colonies
    Cristina Botías
    Laboratorio de Patología Apícola, Centro Apícola Regional CAR, Junta de Comunidades de Castilla La Mancha, San Martín s n 19180, Marchamalo, Guadalajara, Spain
    Parasitol Res 110:2557-61. 2012
    ....
  22. doi request reprint The differential development of microsporidia infecting worker honey bee (Apis mellifera) at increasing incubation temperature
    Mariano Higes
    Centro Apicola Regional, Bee Pathology laboratory, Consejeria de Agricultura, Junta de Comunidades de Castilla La Mancha, 19180 Marchamalo, Spain Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain
    Environ Microbiol Rep 2:745-8. 2010
    ..apis was inhibited. This and other published data suggest that N. ceranae is eurythermal whereas N. apis is stenothermal. The higher temperature tolerance recorded may be related to the higher prevalence of N. ceranae reported worldwide...
  23. doi request reprint A preliminary study of the epidemiological factors related to honey bee colony loss in Spain
    Mariano Higes
    Bee Pathology Laboratory and Hive Products Laboratory, Centro Apicola Regional, JCCM, 19180 Marchamalo, Spain Epidemiology Department, TRAGSEGA, Madrid, Spain Animal Health Department, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain I U CINQUIMA, Analytical Chemistry Group, Facultad de Ciencias, Universidad de Valladolid, 47011 Valladolid, Spain
    Environ Microbiol Rep 2:243-50. 2010
    ..ceranae is a key factor in the colony losses detected over recent years in Spain. However, more detailed studies that permit subgroup analyses will be necessary to contrast these findings...
  24. doi request reprint Horizontal transmission of Nosema ceranae (Microsporidia) from worker honeybees to queens (Apis mellifera)
    Mariano Higes
    Bee Pathology laboratory, Centro Apicola Regional, JCCM, 19180 Marchamalo, Spain Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain
    Environ Microbiol Rep 1:495-8. 2009
    ..The presented data may explain the role of house honeybees in natural queen infection, although it is probably that a high proportion of infected house bees must be required to infect the queen...
  25. doi request reprint High levels of genetic diversity in Nosema ceranae within Apis mellifera colonies
    Tamara Gómez-Moracho
    Laboratorio de Patología Apícola, Centro Apicola Regional, JCCM, 19180 Marchamalo, Spain
    Parasitology 141:475-81. 2014
    ..ceranae infecting honeybee colonies in the field which complicates, and must not be overlooked, further analysis of host-parasite interactions...
  26. doi request reprint Ribosomal gene polymorphism in small genomes: analysis of different 16S rRNA sequences expressed in the honeybee parasite Nosema ceranae (Microsporidia)
    Soledad Sagastume
    Centro Apicola Regional, Bee Pathology laboratory, Marchamalo, Guadalajara, 19180, Spain
    J Eukaryot Microbiol 61:42-50. 2014
    ..The fact that the polymorphisms in such a minimal organism as N. ceranae are maintained in samples collected worldwide suggest that the existence of differently expressed rRNA may play an adaptive role in the microsporidian. ..
  27. ncbi request reprint A sensitive one-step real-time RT-PCR method for detection of deformed wing virus and black queen cell virus in honeybee Apis mellifera
    Deborah Kukielka
    Animal Health Department, Faculty of Veterinary Science, Universidad Complutense de Madrid, Avda Puerta de Hierro s n, 28040 Madrid, Spain
    J Virol Methods 147:275-81. 2008
    ....