Africa Holguin

Summary

Country: Spain

Publications

  1. ncbi request reprint Efficacy of antiretroviral therapy in individuals infected with HIV-1 non-B subtypes
    Africa Holguin
    Department of Infectious Diseases, Hospital Carlos III, Madrid, Spain
    AIDS Rev 8:98-107. 2006
  2. doi request reprint Drug resistance prevalence in human immunodeficiency virus type 1 infected pediatric populations in Honduras and El Salvador during 1989-2009
    Africa Holguin
    HIV 1 Molecular Epidemiology Laboratory, Department of Microbiology and Parasitology, FiBIO IRYCIS Hospital Ramón y Cajal and CIBER ESP, Madrid, Spain
    Pediatr Infect Dis J 30:e82-7. 2011
  3. ncbi request reprint Natural polymorphisms in the protease gene modulate the replicative capacity of non-B HIV-1 variants in the absence of drug pressure
    Africa Holguin
    Service of Infectious Diseases, Hospital Carlos III, Madrid, Spain
    J Clin Virol 36:264-71. 2006
  4. ncbi request reprint Amino acid conservation in the gp41 transmembrane protein and natural polymorphisms associated with enfuvirtide resistance across HIV-1 variants
    Africa Holguin
    Service of Infectious Diseases, Hospital Carlos III, Madrid, Spain
    AIDS Res Hum Retroviruses 23:1067-74. 2007
  5. ncbi request reprint Susceptibility of HIV-1 non-B subtypes and recombinant variants to Enfuvirtide
    Africa Holguin
    Service of Infectious Diseases, Hospital Carlos III, Madrid, Spain
    J Clin Virol 38:176-80. 2007
  6. ncbi request reprint Differences in the length of gag proteins among different HIV type 1 subtypes
    Africa Holguin
    Department of Infectious Diseases, Hospital Carlos III, 28029 Madrid, Spain
    AIDS Res Hum Retroviruses 21:886-93. 2005
  7. doi request reprint Genetic characterization of complex inter-recombinant HIV-1 strains circulating in Spain and reliability of distinct rapid subtyping tools
    Africa Holguin
    Department of Infectious Diseases, Hospital Carlos III, Madrid, Spain
    J Med Virol 80:383-91. 2008
  8. ncbi request reprint Heterogeneous nature of HIV-1 recombinants spreading in Spain
    Africa Holguin
    Department of Infectious Diseases, Hospital Carlos III, Madrid, Spain
    J Med Virol 75:374-80. 2005
  9. pmc Performance of three commercial viral load assays, Versant human immunodeficiency virus type 1 (HIV-1) RNA bDNA v3.0, Cobas AmpliPrep/Cobas TaqMan HIV-1, and NucliSens HIV-1 EasyQ v1.2, testing HIV-1 non-B subtypes and recombinant variants
    Africa Holguin
    Department of Infectious Diseases, Hospital Carlos III, Madrid, Spain
    J Clin Microbiol 46:2918-23. 2008
  10. ncbi request reprint Increase of non-B subtypes and recombinants among newly diagnosed HIV-1 native Spaniards and immigrants in Spain
    Africa Holguin
    HIV 1 Molecular Epidemiology Group, Service of Infectious Diseases, Hospital Carlos III, Madrid, Spain
    Curr HIV Res 6:327-34. 2008

Collaborators

Detail Information

Publications41

  1. ncbi request reprint Efficacy of antiretroviral therapy in individuals infected with HIV-1 non-B subtypes
    Africa Holguin
    Department of Infectious Diseases, Hospital Carlos III, Madrid, Spain
    AIDS Rev 8:98-107. 2006
    ..All these aspects explain why the response to antiretroviral therapy might vary in subjects infected with different HIV-1 clades...
  2. doi request reprint Drug resistance prevalence in human immunodeficiency virus type 1 infected pediatric populations in Honduras and El Salvador during 1989-2009
    Africa Holguin
    HIV 1 Molecular Epidemiology Laboratory, Department of Microbiology and Parasitology, FiBIO IRYCIS Hospital Ramón y Cajal and CIBER ESP, Madrid, Spain
    Pediatr Infect Dis J 30:e82-7. 2011
    ..Human immunodeficiency virus type-1 (HIV-1) variants and drug-resistance mutations were identified in naive and antiretroviral drug-experienced children with virologic failure, in Honduras and El Salvador...
  3. ncbi request reprint Natural polymorphisms in the protease gene modulate the replicative capacity of non-B HIV-1 variants in the absence of drug pressure
    Africa Holguin
    Service of Infectious Diseases, Hospital Carlos III, Madrid, Spain
    J Clin Virol 36:264-71. 2006
    ..Several amino acid substitutions occur frequently in some non-B viruses at positions associated with drug resistance in clade B viruses. The clinical relevance of those polymorphisms is unclear...
  4. ncbi request reprint Amino acid conservation in the gp41 transmembrane protein and natural polymorphisms associated with enfuvirtide resistance across HIV-1 variants
    Africa Holguin
    Service of Infectious Diseases, Hospital Carlos III, Madrid, Spain
    AIDS Res Hum Retroviruses 23:1067-74. 2007
    ....
  5. ncbi request reprint Susceptibility of HIV-1 non-B subtypes and recombinant variants to Enfuvirtide
    Africa Holguin
    Service of Infectious Diseases, Hospital Carlos III, Madrid, Spain
    J Clin Virol 38:176-80. 2007
    ..Data on susceptibility of HIV-1 non-B subtypes to Enfuvirtide (ENF) is rather limited...
  6. ncbi request reprint Differences in the length of gag proteins among different HIV type 1 subtypes
    Africa Holguin
    Department of Infectious Diseases, Hospital Carlos III, 28029 Madrid, Spain
    AIDS Res Hum Retroviruses 21:886-93. 2005
    ..The effect of Gag variation on the viral cycle among different HIV-1 clades needs to be further investigated...
  7. doi request reprint Genetic characterization of complex inter-recombinant HIV-1 strains circulating in Spain and reliability of distinct rapid subtyping tools
    Africa Holguin
    Department of Infectious Diseases, Hospital Carlos III, Madrid, Spain
    J Med Virol 80:383-91. 2008
    ..This is the first description of CRF02_AG/CRF06_ cpx recombinants in Spain. The results highlight the tremendous heterogeneity of HIV-1 recombinant strains currently in circulation...
  8. ncbi request reprint Heterogeneous nature of HIV-1 recombinants spreading in Spain
    Africa Holguin
    Department of Infectious Diseases, Hospital Carlos III, Madrid, Spain
    J Med Virol 75:374-80. 2005
    ..This study demonstrates that most viruses circulating in Spain with clade G sequences at the pol-coding region are in fact inter-subtype recombinants, with CRF02_AG being the most prevalent virus...
  9. pmc Performance of three commercial viral load assays, Versant human immunodeficiency virus type 1 (HIV-1) RNA bDNA v3.0, Cobas AmpliPrep/Cobas TaqMan HIV-1, and NucliSens HIV-1 EasyQ v1.2, testing HIV-1 non-B subtypes and recombinant variants
    Africa Holguin
    Department of Infectious Diseases, Hospital Carlos III, Madrid, Spain
    J Clin Microbiol 46:2918-23. 2008
    ..Our data emphasize the need to use the same method for monitoring patients on therapy and also the convenience of HIV-1 subtyping...
  10. ncbi request reprint Increase of non-B subtypes and recombinants among newly diagnosed HIV-1 native Spaniards and immigrants in Spain
    Africa Holguin
    HIV 1 Molecular Epidemiology Group, Service of Infectious Diseases, Hospital Carlos III, Madrid, Spain
    Curr HIV Res 6:327-34. 2008
    ..HIV-1 non-B subtypes are increasingly present among newly diagnosed HIV-1 individuals in Madrid, representing a third of cases in 2007, whereas 10% of newly diagnosed HIV-1 native Spaniards had non-B viruses...
  11. pmc Reliability of rapid subtyping tools compared to that of phylogenetic analysis for characterization of human immunodeficiency virus type 1 non-B subtypes and recombinant forms
    Africa Holguin
    Department of Microbiology, HIV 1 Molecular Epidemiology Laboratory, Hospital Carlos III, Madrid, Spain
    J Clin Microbiol 46:3896-9. 2008
    ..Before entering routine clinical use, rapid subtyping tools should be optimized and updated periodically. Larger numbers of different non-B subtypes and CRF sequences should be included...
  12. doi request reprint Performance of OraQuick Advance Rapid HIV-1/2 Antibody Test for detection of antibodies in oral fluid and serum/plasma in HIV-1+ subjects carrying different HIV-1 subtypes and recombinant variants
    Africa Holguin
    Department of Infectious Diseases, Hospital Carlos III, Madrid, Spain
    J Clin Virol 45:150-2. 2009
    ..Rapid, simple, low-cost, sensitive, and specific tests are needed to detect antibodies to all HIV-1 subtypes, especially in developing countries...
  13. doi request reprint Clinical differences and viral diversity between newly HIV type 1-diagnosed African and non-African patients in Spain (2005-2007)
    Gonzalo Yebra
    HIV 1 Molecular Epidemiology Laboratory, Service of Microbiology, Hospital Ramón y Cajal and CIBER ESP, Madrid, Spain
    AIDS Res Hum Retroviruses 25:37-44. 2009
    ..More advanced immunodeficiency and the coexistence of parasitic diseases and infections with a large diversity of HIV-1 non-B and recombinant variants are expected...
  14. ncbi request reprint Molecular and epidemiological characteristics of blood-borne virus infections among recent immigrants in Spain
    Carlos Toro
    Service of Infectious Diseases, Hospital Carlos III, Madrid, Spain
    J Med Virol 78:1599-608. 2006
    ..In contrast, Latin Americans with high-risk sexual practices may be a particularly vulnerable collective to acquire blood-borne viruses in the receptor country...
  15. doi request reprint Trends in drug resistance prevalence in HIV-1-infected children in Madrid: 1993 to 2010 analysis
    Miguel de Mulder
    HIV 1 Molecular Epidemiology Laboratory, Microbiology and Parasitology Unit, Hospital Universitario Ramón y Cajal IRyCIS and CIBER ESP, Spain
    Pediatr Infect Dis J 31:e213-21. 2012
    ..Drug resistance mutations compromise antiretroviral treatment (ART) effectiveness in HIV-1-infected children. Trends in drug resistance prevalence have not been previously evaluated in HIV-infected children in Spain...
  16. ncbi request reprint Genetic analysis of regulatory, promoter, and TAR regions of LTR sequences belonging to HIV type 1 Non-B subtypes
    Eva Ramírez de Arellano
    Department of Infectious Diseases, Hospital Carlos III, 28035 Madrid Spain
    AIDS Res Hum Retroviruses 21:949-54. 2005
    ..To our knowledge, this is the first characterization of LTR sequences belonging to subtypes J and H...
  17. ncbi request reprint High prevalence of HIV-1 subtype G and natural polymorphisms at the protease gene among HIV-infected immigrants in Madrid
    Africa Holguin
    Service of Infectious Diseases, Hospital Carlos III, Instituto de Salud Carlos III, Madrid, Spain
    AIDS 16:1163-70. 2002
    ..Genetic characterization of HIV-1 subtypes among immigrants and natives infected overseas...
  18. doi request reprint Drastic decrease of transcription activity due to hypermutated long terminal repeat (LTR) region in different HIV-1 subtypes and recombinants
    Eva Ramírez de Arellano
    Department of Infectious Diseases, Hospital Carlos III, Madrid, Spain National Center for Microbiology, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain
    Antiviral Res 88:152-9. 2010
    ..G to A hypermutation at some important transcription binding-factor sites within LTR compromised the activity of the viral promoter, decreasing the in vitro viral transcription of the luciferase gene...
  19. ncbi request reprint Prevalence of HIV-1 non-B subtypes, syphilis, HTLV, and hepatitis B and C viruses among immigrant sex workers in Madrid, Spain
    Maite Gutiérrez
    Microbiology Unit, Hospital Carlos III, Madrid, Spain
    J Med Virol 74:521-7. 2004
    ..A relatively high proportion of immigrant sex workers in Madrid were infected with HIV-1 and syphilis, whereas infections with hepatitis viruses or HTLV were uncommon...
  20. ncbi request reprint HIV-positive immigrants in the Canary Islands, Spain: implications for public health in Europe
    Africa Holguin
    Service of Infectious Diseases, Hospital Carlos III, Madrid
    HIV Clin Trials 4:184-92. 2003
    ..The presence of HIV-1 non-B variants has already been reported in Spain and other European countries, mostly among African immigrants...
  21. ncbi request reprint Impact of ethnicity and HIV type 1 subtype on response to first-line antiretroviral therapy
    Eva Ramírez de Arellano
    Department of Infectious Diseases, Hospital Carlos III, Madrid, Spain
    AIDS Res Hum Retroviruses 23:891-4. 2007
    ..In a multivariate analysis neither HIV subtype nor ethnicity was associated with different CD4 gains at any given time point, suggesting that reconstitution of CD4+ T cells under HAART is not influenced by ethnicity...
  22. pmc Description of HIV-1 group M molecular epidemiology and drug resistance prevalence in Equatorial Guinea from migrants in Spain
    Gonzalo Yebra
    HIV 1 Molecular Epidemiology Laboratory, Dept of Microbiology, Hospital Ramón y Cajal IRYCIS and CIBERESP, Madrid, Spain
    PLoS ONE 8:e64293. 2013
    ..We aimed to describe the HIV-1 group M (HIV-1M) variants and drug-resistance mutations in GQ using sequences sampled in this country and in Spain, a frequent destination of Equatoguinean migrants...
  23. ncbi request reprint HIV-1 subtype J recombinant viruses in Spain
    Africa Holguin
    Service of Infectious Diseases, Hospital Carlos III, Instituto de Salud Carlos III, 28035 Madrid, Spain
    AIDS Res Hum Retroviruses 18:523-9. 2002
    ..However, env sequences clustered with subtypes A and C, respectively. This work represents the first characterization of HIV-1 containing subtype J-like genomic regions in Spain...
  24. ncbi request reprint Impact of frequent natural polymorphisms at the protease gene on the in vitro susceptibility to protease inhibitors in HIV-1 non-B subtypes
    Africa Holguin
    Service of Infectious Diseases, Hospital Carlos III, Madrid, Spain
    J Clin Virol 31:215-20. 2004
    ....
  25. pmc Most HIV type 1 non-B infections in the Spanish cohort of antiretroviral treatment-naïve HIV-infected patients (CoRIS) are due to recombinant viruses
    Gonzalo Yebra
    HIV 1 Molecular Epidemiology Laboratory, Microbiology Department, Hospital Ramon y Cajal, Madrid, Spain
    J Clin Microbiol 50:407-13. 2012
    ..An increase in the frequency of mosaic viruses, reflecting the increasing heterogeneity of the HIV epidemic in our country, is expected...
  26. ncbi request reprint Introduction of non-B subtypes among Spaniards newly diagnosed with HIV type 1 in the Canary Islands
    Africa Holguin
    Service of Infectious Diseases, Hospital Carlos III, Calle Sinesio Delgado 10, 28029 Madrid, Spain
    AIDS Res Hum Retroviruses 23:498-502. 2007
    ..HIV-1 non-B subtypes are frequently recognized in HIV+ Africans arriving in the Canary islands and continuously introduced into the newly diagnosed HIV-1+ native population living in the archipelago...
  27. ncbi request reprint [Regulation of transcription in different HIV-1 subtypes]
    Eva Ramirez de Arellano
    Laboratorio de Biologia Molecular, Servicio de Enfermedades Infecciosas, Hospital Carlos III, Madrid, Spain
    Enferm Infecc Microbiol Clin 23:156-62. 2005
    ..In some circumstances, a relationship between replication capacity in vitro and changes in binding sequences for transcription factors located at the LTR has been proven...
  28. pmc High drug resistance prevalence among vertically HIV-infected patients transferred from pediatric care to adult units in Spain
    Miguel de Mulder
    HIV 1 Molecular Epidemiology Laboratory, Microbiology and Parasitology Department, Hospital Universitario Ramon y Cajal, IRYCIS and CIBER ESP, Madrid, Spain
    PLoS ONE 7:e52155. 2012
    ....
  29. doi request reprint Drug resistance prevalence and HIV-1 variant characterization in the naive and pretreated HIV-1-infected paediatric population in Madrid, Spain
    Miguel de Mulder
    HIV 1 Molecular Epidemiology Laboratory, FiBIO IRYCIS, Hospital Ramon y Cajal, Madrid, Spain
    J Antimicrob Chemother 66:2362-71. 2011
    ..HIV-1 variants and drug resistance mutations were identified in HIV-infected children from Madrid, Spain...
  30. ncbi request reprint Variability in the P6gag domains of HIV-1 involved in viral budding
    Africa Holguin
    Service of Infectious Diseases, Hospital Carlos III, Madrid, Spain
    AIDS 20:624-7. 2006
    ..P6gag variability across HIV-1 subtypes and after antiretroviral exposure may influence interactions with host cells involved in viral budding...
  31. ncbi request reprint Resistance to antiretroviral agents in individuals with HIV-1 non-B subtypes
    Africa Holguin
    Service of Infectious Diseases, Instituto de Salud Carlos III, Madrid, Spain
    HIV Clin Trials 3:403-11. 2002
    ....
  32. doi request reprint Phylogenetic and demographic characterization of HIV-1 transmission in Madrid, Spain
    Gonzalo Yebra
    HIV 1 Molecular Epidemiology Laboratory, Department of Microbiology, Hospital Ramón y Cajal IRYCIS and CIBERESP, Ctra Colmenar Viejo, Km 9 100, 28034 Madrid, Spain
    Infect Genet Evol 14:232-9. 2013
    ..The Community of Madrid represents a challenge for the control of HIV epidemic in Spain given its high HIV prevalence and increasing proportion of immigrant people among HIV-infected population...
  33. pmc Molecular surveillance of HIV-1 in Madrid, Spain: a phylogeographic analysis
    José M González-Alba
    Servicio de Microbiologia, Instituto Ramón y Cajal de Investigación Sanitaria IRYCIS, Madrid, Spain
    J Virol 85:10755-63. 2011
    ..These results suggest that the HIV-1 epidemic will evolve toward a more complex epidemiological landscape...
  34. ncbi request reprint Genetic analysis of the long terminal repeat (LTR) promoter region in HIV-1-infected individuals with different rates of disease progression
    Eva Ramirez de Arellano
    Department of Infectious Diseases, Hospital Carlos III, C Sinesio Delgado, 10, 28029, Madrid, Spain
    Virus Genes 34:111-6. 2007
    ..This is the first study examining the genetic variability of the HIV-1 LTR region in long-term non-progressors showing further divergent outcomes...
  35. pmc Increase of transmitted drug resistance among HIV-infected sub-Saharan Africans residing in Spain in contrast to the native population
    Gonzalo Yebra
    HIV 1 Molecular Epidemiology Laboratory, Microbiology Department, IRYCIS Hospital Ramón y Cajal and CIBER ESP, Madrid, Spain
    PLoS ONE 6:e26757. 2011
    ..The prevalence of transmitted HIV drug resistance (TDR) is stabilizing or decreasing in developed countries. However, this trend is not specifically evaluated among immigrants from regions without well-implemented antiretroviral strategies...
  36. ncbi request reprint The maturation inhibitor bevirimat (PA-457) can be active in patients carrying HIV type-1 non-B subtypes and recombinants
    Gonzalo Yebra
    HIV 1 Molecular Epidemiology Laboratory, Department of Microbiology, Hospital Universitario Ramón y Cajal and CIBERESP, Madrid, Spain
    Antivir Ther 13:1083-5. 2008
    ..Thus, genotypic resistance in non-B subtypes still requires analysis...
  37. ncbi request reprint Evaluation of three rapid tests for detection of antibodies to HIV-1 non-B subtypes
    Africa Holguin
    Infectious Diseases Department, Hospital Carlos III, Calle Sinesio Delgado 10, 28029 Madrid, Spain
    J Virol Methods 115:105-7. 2004
    ..The Determine HIV-1/2, Equipar HIV, and Multispot HIV-1/2 tests were 100% sensitive and specific for the detection of HIV-1 antibodies. Multispot showed 92.7% specificity for HIV-2...
  38. ncbi request reprint New findings on transcription regulation across different HIV-1 subtypes
    Eva Ramirez de Arellano
    Department of Infectious Diseases, Hospital Carlos III, Madrid, Spain
    AIDS Rev 8:9-16. 2006
    ..Finally, the knowledge of the molecular mechanisms of transcription regulation events could help in the search for new compounds targeting the critical steps of viral transcription...
  39. doi request reprint [Epidemiology of drug-resistant HIV-1 transmission in naïve patients in Spain]
    Gonzalo Yebra
    Laboratorio de Epidemiología Molecular del virus de la inmunodeficiencia humana de tipo 1, Servicio de Microbiologia, Hospital Ramon y Cajal, CIBER ESP, Madrid, Spain
    Med Clin (Barc) 135:561-7. 2010
    ....
  40. pmc Dried blood as an alternative to plasma or serum for Trypanosoma cruzi IgG detection in screening programs
    Africa Holguin
    HIV 1 Molecular Epidemiology Laboratory, Microbiology Department, IRYCIS Hospital Ramón y Cajal and CIBER ESP, Madrid, Spain
    Clin Vaccine Immunol 20:1197-202. 2013
    ..This method may also reduce the false-negative rates observed with some rapid diagnostic tests. The lower relative sensitivity compared to the reference method may be increased by lowering the optical density threshold. ..
  41. doi request reprint Prevalence of primary resistance mutations to integrase inhibitors in treatment-naïve and -experienced patients infected with B and non-B HIV-1 variants
    Carolina Gutierrez
    Infectious Diseases Department, Hospital Universitario Ramón y Cajal and IRYCIS, Madrid, Spain
    HIV Clin Trials 14:10-6. 2013
    ..Secondary mutations with little effect on INSTI susceptibility and additional substitutions with an uncertain role have also been described especially in HIV-1 non-B variants...