Jose M Guisan


Country: Spain


  1. Pérez M, Gonçalves E, Salgado J, Rocha M, Almeida P, Vici A, et al. Production of Omegas-6 and 9 from the Hydrolysis of Açaí and Buriti Oils by Lipase Immobilized on a Hydrophobic Support. Molecules. 2018;23: pubmed publisher
    ..The results showed the potential of these immobilized lipases to obtain omegas-6 and 9 from Brazilian natural oils. This work may improve the enzymatic methodologies for obtaining foods and drugs enriched with fatty acids. ..
  2. Abreu Silveira E, Moreno Perez S, Basso A, Serban S, Pestana Mamede R, Tardioli P, et al. Biocatalyst Engineering of Thermomyces Lanuginosus Lipase Adsorbed on Hydrophobic Supports: Modulation of Enzyme Properties for Ethanolysis of Oil in Solvent-Free Systems. J Biotechnol. 2018;: pubmed publisher
    ..Macroporous and highly hydrophobic supports (e.g., Purolite C-18) seem to be very useful to prepare optimal immobilized biocatalysts for ethanolysis of oils by TLL in solvent-free systems. ..
  3. Rocha Martin J, Acosta A, Berenguer J, Guisan J, Lopez Gallego F. Selective oxidation of glycerol to 1,3-dihydroxyacetone by covalently immobilized glycerol dehydrogenases with higher stability and lower product inhibition. Bioresour Technol. 2014;170:445-453 pubmed publisher
    ..This is one of the few examples where the same immobilization protocol has minimized enzyme product inhibition and maximized thermal stability. ..
  4. Fernandez Lorente G, Bolivar J, Rocha Martin J, Curiel J, Muñoz R, de las Rivas B, et al. Synthesis of propyl gallate by transesterification of tannic acid in aqueous media catalysed by immobilised derivatives of tannase from Lactobacillus plantarum. Food Chem. 2011;128:214-7 pubmed publisher
    ..0. The product was chromatographically pure, and the reaction by-product was 55% pure gallic acid. On the other hand, immobilised tannase was fairly stable under optimal reaction conditions. ..
  5. García García P, Rocha Martin J, Fernandez Lorente G, Guisan J. Co-localization of oxidase and catalase inside a porous support to improve the elimination of hydrogen peroxide: Oxidation of biogenic amines by amino oxidase from Pisum sativum. Enzyme Microb Technol. 2018;115:73-80 pubmed publisher
    ..When enzymes are immobilized in close proximity to each other in a co-localized pattern, the generation of byproducts as H2O2 is strongly reduced. ..
  6. Romero Fernández M, Moreno Perez S, Martins de Oliveira S, Santamaría R, Guisan J, Rocha Martin J. Preparation of a robust immobilized biocatalyst of ?-1,4-endoxylanase by surface coating with polymers for production of xylooligosaccharides from different xylan sources. N Biotechnol. 2018;44:50-58 pubmed publisher
    ..Maximum values of xylooligosaccharides were found for beechwood at 20.6?mg/mL, wheat at 12.5?mg/mL and corncob at 30.4?mg/mL. The optimized biocatalyst was reused for 15 reaction cycles without affecting its catalytic activity. ..
  7. Benavente R, Pessela B, Curiel J, de las Rivas B, Muñoz R, Guisán J, et al. Improving Properties of a Novel β-Galactosidase from Lactobacillus plantarum by Covalent Immobilization. Molecules. 2015;20:7874-89 pubmed publisher
  8. Orrego A, Ghobadi R, Moreno Perez S, Mendoza A, Fernandez Lorente G, Guisan J, et al. Stabilization of Immobilized Lipases by Intense Intramolecular Cross-Linking of Their Surfaces by Using Aldehyde-Dextran Polymers. Int J Mol Sci. 2018;19: pubmed publisher
    ..The TLL and the CALB were 40-fold and 4-fold more stable than the unmodified conjugate. ..
  9. Rocha Martin J, Vega D, Bolivar J, Godoy C, Hidalgo A, Berenguer J, et al. New biotechnological perspectives of a NADH oxidase variant from Thermus thermophilus HB27 as NAD+-recycling enzyme. BMC Biotechnol. 2011;11:101 pubmed publisher
    ..The high stability along with its capability to be reactivated makes this biocatalyst highly re-useable for cofactor recycling in redox biotransformations. ..

More Information


  1. Turati D, Morais Júnior W, Terrasan C, Moreno Perez S, Pessela B, Fernandez Lorente G, et al. Immobilization of Lipase from Penicillium sp. Section Gracilenta (CBMAI 1583) on Different Hydrophobic Supports: Modulation of Functional Properties. Molecules. 2017;22: pubmed publisher
    ..Despite having identical mechanisms of immobilization, different hydrophobic supports seem to promote different shapes of the adsorbed open active site of the lipase and hence different functional properties. ..
  2. Moreno Pérez S, Orrego A, Romero Fernández M, Trobo Maseda L, Martins DeOliveira S, Munilla R, et al. Intense PEGylation of Enzyme Surfaces: Relevant Stabilizing Effects. Methods Enzymol. 2016;571:55-72 pubmed publisher
  3. Orrego A, Trobo Maseda L, Rocha Martin J, Guisan J. Immobilization-stabilization of a complex multimeric sucrose synthase from Nitrosomonas europaea. Synthesis of UDP-glucose. Enzyme Microb Technol. 2017;105:51-58 pubmed publisher
    ..This optimal biocatalyst was modified with a polycationic polymer, polyethyleneimine (PEI), increasing its stability in the presence of the organic co-solvents necessary to glycosylate apolar antioxidants by GTs coupled to SuSy. ..
  4. Pereira M, Velasco Lozano S, Moreno Perez S, Polizeli A, Heinen P, Facchini F, et al. Different Covalent Immobilizations Modulate Lipase Activities of Hypocrea pseudokoningii. Molecules. 2017;22: pubmed publisher
    ..This work shows the importance of immobilization as a tool to increase the lipase stability to temperature and organic solvents, thus enabling the possibility of their application at large scale processes. ..
  5. Trobo Maseda L, Orrego A, Moreno Perez S, Fernandez Lorente G, Guisan J, Rocha Martin J. Stabilization of multimeric sucrose synthase from Acidithiobacillus caldus via immobilization and post-immobilization techniques for synthesis of UDP-glucose. Appl Microbiol Biotechnol. 2018;102:773-787 pubmed publisher
    ..This biocatalyst produced 256 mM of UDP-glucose in a single batch, accumulating 1 M after five reaction cycles. Therefore, this immobilized enzyme can be of great interest as a biocatalyst to synthesize UDP-glucose. ..
  6. Abreu Silveira E, Moreno Perez S, Basso A, Serban S, Pestana Mamede R, Tardioli P, et al. Modulation of the regioselectivity of Thermomyces lanuginosus lipase via biocatalyst engineering for the Ethanolysis of oil in fully anhydrous medium. BMC Biotechnol. 2017;17:88 pubmed publisher
    ..The absence of acyl migrations under identical reaction conditions is clearly observed and hence the different activities and regioselectivities seem to be due to the different catalytic properties of different derivatives of TLL. ..