J M Gonzalez

Summary

Country: Spain

Publications

  1. ncbi request reprint Counterselection of prokaryotic ribosomal RNA during reverse transcription using non-random hexameric oligonucleotides
    J M Gonzalez
    IRNAS CSIC, Avda Reina Mercedes 10, 41012 Sevilla, Spain
    J Microbiol Methods 71:288-91. 2007
  2. pmc Microbial community fingerprinting by differential display-denaturing gradient gel electrophoresis
    M C Portillo
    Instituto de Recursos Naturales y Agrobiologia, IRNAS CSIC, Seville, Spain
    Appl Environ Microbiol 77:351-4. 2011
  3. ncbi request reprint Bacterial degradation of dichloromethane in cultures and natural environments
    Valentina I Krausova
    Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region 142290, Russia
    J Microbiol Methods 54:419-22. 2003
  4. pmc Amplification by PCR artificially reduces the proportion of the rare biosphere in microbial communities
    Juan M Gonzalez
    Institute of Natural Resources and Agrobiology, Spanish Council for Research, IRNAS CSIC, Sevilla, Spain
    PLoS ONE 7:e29973. 2012
  5. ncbi request reprint Metabolically active Crenarchaeota in Altamira Cave
    Juan M Gonzalez
    Instituto de Recursos Naturales y Agrobiologia, CSIC, Apartado 1052, 41080 Sevilla, Spain
    Naturwissenschaften 93:42-5. 2006
  6. ncbi request reprint Multiple displacement amplification as a pre-polymerase chain reaction (pre-PCR) to process difficult to amplify samples and low copy number sequences from natural environments
    Juan M Gonzalez
    Instituto de Recursos Naturales y Agrobiologia, CSIC, Apartado, Sevilla, Spain
    Environ Microbiol 7:1024-8. 2005
  7. ncbi request reprint Pectinatus portalensis nov. sp., a relatively fast-growing, coccoidal, novel Pectinatus species isolated from a wastewater treatment plant
    Juan M Gonzalez
    Instituto de Recursos Naturales y Agrobiologia, CSIC, Apartado 1052, 41080 Sevilla, Spain
    Antonie Van Leeuwenhoek 86:241-7. 2004
  8. ncbi request reprint A simple fluorimetric method for the estimation of DNA-DNA relatedness between closely related microorganisms by thermal denaturation temperatures
    Juan M Gonzalez
    Instituto de Recursos Naturales y Agrobiologia, CSIC, P O Box 1052, 41080 Sevilla, Spain
    Extremophiles 9:75-9. 2005
  9. ncbi request reprint Evaluating putative chimeric sequences from PCR-amplified products
    Juan M Gonzalez
    Instituto de Recursos Naturales y Agrobiologia, CSIC Apartado 1052, 41080 Sevilla, Spain
    Bioinformatics 21:333-7. 2005
  10. ncbi request reprint Microbial diversity in biodeteriorated monuments as studied by denaturing gradient gel electrophoresis
    Juan M Gonzalez
    Instituto de Recursos Naturales y Agrobiologia, CSIC, Apdo 1052, 41080 Sevilla, Spain
    J Sep Sci 27:174-80. 2004

Collaborators

  • C Saiz-Jimenez
  • F Almazan
  • Alex Mira
  • C Smerdou
  • M Reina
  • I Groth
  • F Raposo
  • S Alonso
  • L I Evtushenko
  • C Sanchez
  • M C Portillo
  • L Laiz
  • S Sanchez-Moral
  • V Jurado
  • V Sririn
  • W Kanoksilapatham
  • S Cuezva
  • L Enjuanes
  • B Rincon
  • M Shahamat
  • A Ortiz-Martinez
  • Valentina I Krausova
  • E Calvo
  • I Sola
  • M Santana
  • Z Penzes
  • A Izeta
  • A Corzo
  • D Villahermosa
  • R Alloza
  • E Porca
  • A Dionisio
  • A Z Miller
  • J C Cañaveras
  • E Akatova
  • M F Macedo
  • L Serrano
  • R Borja
  • V Soler
  • M Hernandez-Marine
  • D Benavente
  • M Valens
  • L Luque
  • J E M Watts
  • K R Sowers
  • M Alavi
  • D W Maeder
  • F T Robb
  • Frank T Robb
  • L L Peters
  • J M Sanchez
  • J Ortego
  • D Escors
  • C Riquelme
  • S L Alper
  • M Hanspal
  • N Mohandas
  • K M John
  • C Brugnara
  • B Gwynn
  • S C Liu
  • S H Orkin
  • S E Lux
  • R A Shivdasani

Detail Information

Publications31

  1. ncbi request reprint Counterselection of prokaryotic ribosomal RNA during reverse transcription using non-random hexameric oligonucleotides
    J M Gonzalez
    IRNAS CSIC, Avda Reina Mercedes 10, 41012 Sevilla, Spain
    J Microbiol Methods 71:288-91. 2007
    ..We designed specific hexamers (HD/DHTTTT) to prime reverse transcription reactions resulting in a selective synthesis of cDNA corresponding to mRNA from prokaryotic total RNA extractions...
  2. pmc Microbial community fingerprinting by differential display-denaturing gradient gel electrophoresis
    M C Portillo
    Instituto de Recursos Naturales y Agrobiologia, IRNAS CSIC, Seville, Spain
    Appl Environ Microbiol 77:351-4. 2011
    ..By adding a nucleotide to the 3' ends of PCR primers, 16 primer pairs and fingerprints were generated per community. Complexity reduction in each partial fingerprint facilitates sample comparison...
  3. ncbi request reprint Bacterial degradation of dichloromethane in cultures and natural environments
    Valentina I Krausova
    Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region 142290, Russia
    J Microbiol Methods 54:419-22. 2003
    ..Herein, we present an original and easy spectrophotometric method to estimate DCM concentrations in cultures and environmental samples during DCM biodegradation experiments...
  4. pmc Amplification by PCR artificially reduces the proportion of the rare biosphere in microbial communities
    Juan M Gonzalez
    Institute of Natural Resources and Agrobiology, Spanish Council for Research, IRNAS CSIC, Sevilla, Spain
    PLoS ONE 7:e29973. 2012
    ..The structure of microbial communities from PCR-based surveys is clearly biased against low abundant taxa which are required to decipher the complete extent of microbial diversity in nature...
  5. ncbi request reprint Metabolically active Crenarchaeota in Altamira Cave
    Juan M Gonzalez
    Instituto de Recursos Naturales y Agrobiologia, CSIC, Apartado 1052, 41080 Sevilla, Spain
    Naturwissenschaften 93:42-5. 2006
    ..Although low-temperature Crenarchaeota detected in a variety of ecosystems by using molecular techniques remain uncultured, this RNA-based study confirms an active participation of the Crenarchaeota in cave biogeochemical cycles...
  6. ncbi request reprint Multiple displacement amplification as a pre-polymerase chain reaction (pre-PCR) to process difficult to amplify samples and low copy number sequences from natural environments
    Juan M Gonzalez
    Instituto de Recursos Naturales y Agrobiologia, CSIC, Apartado, Sevilla, Spain
    Environ Microbiol 7:1024-8. 2005
    ....
  7. ncbi request reprint Pectinatus portalensis nov. sp., a relatively fast-growing, coccoidal, novel Pectinatus species isolated from a wastewater treatment plant
    Juan M Gonzalez
    Instituto de Recursos Naturales y Agrobiologia, CSIC, Apartado 1052, 41080 Sevilla, Spain
    Antonie Van Leeuwenhoek 86:241-7. 2004
    ..portalensis nov. sp. Both strain B6 and the two described species of Pectinatus grow on beers and wines. These results provide insights about the origin and reservoirs of Pectinatus species and spoiling alcoholic beverages...
  8. ncbi request reprint A simple fluorimetric method for the estimation of DNA-DNA relatedness between closely related microorganisms by thermal denaturation temperatures
    Juan M Gonzalez
    Instituto de Recursos Naturales y Agrobiologia, CSIC, P O Box 1052, 41080 Sevilla, Spain
    Extremophiles 9:75-9. 2005
    ..The method proves to be an easy, rapid, and inexpensive alternative to estimate DNA-DNA relatedness between closely related species...
  9. ncbi request reprint Evaluating putative chimeric sequences from PCR-amplified products
    Juan M Gonzalez
    Instituto de Recursos Naturales y Agrobiologia, CSIC Apartado 1052, 41080 Sevilla, Spain
    Bioinformatics 21:333-7. 2005
    ..Thus, chimeric sequences could lead to the discovery of non-existent microbial species and false diversity estimates...
  10. ncbi request reprint Microbial diversity in biodeteriorated monuments as studied by denaturing gradient gel electrophoresis
    Juan M Gonzalez
    Instituto de Recursos Naturales y Agrobiologia, CSIC, Apdo 1052, 41080 Sevilla, Spain
    J Sep Sci 27:174-80. 2004
    ..In this report, we describe DGGE profiling techniques, show the most common applications, and present some examples of their use in microbial diversity studies on cultural heritage...
  11. ncbi request reprint An efficient strategy for screening large cloned libraries of amplified 16S rDNA sequences from complex environmental communities
    J M Gonzalez
    Instituto de Recursos Naturales y Agrobiologia, Consejo Superior de Investigaciones Cientificas, P O Box 1052, 41080, Seville, Spain
    J Microbiol Methods 55:459-63. 2003
    ..It consists of processing sets of multiple clones simultaneously. This strategy saves up to 90% of the costs and labor spent in the process of screening a 16S rDNA library...
  12. ncbi request reprint Optical thermal cycler for use as a fluorimetric plate reader to estimate DNA concentrations
    Juan M Gonzalez
    Instituto de Recursos Naturales y Agrobiología CSIC, Apdo 1052, 41080, Sevilla, Spain
    Biotechniques 34:710-2. 2003
  13. ncbi request reprint A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature
    J M Gonzalez
    Instituto de Recursos Naturales y Agrobiologia, CSIC, Apartado 1052, 41080 Sevilla, Spain
    Environ Microbiol 4:770-3. 2002
    ..This protocol proves to be a rapid and inexpensive method to estimate DNA base ratios of novel microorganisms...
  14. ncbi request reprint Metabolically active microbial communities of yellow and grey colonizations on the walls of Altamira Cave, Spain
    M C Portillo
    Instituto de Recursos Naturales y Agrobiologia, CSIC, Avda Reina Mercedes, Sevilla, Spain
    J Appl Microbiol 104:681-91. 2008
    ..To determine the major components of total and metabolically active microbial communities of yellow and grey colonizations threatening the conservation of palaeolithic paintings in Altamira Cave (Spain)...
  15. doi request reprint Isolation of five Rubrobacter strains from biodeteriorated monuments
    L Laiz
    Instituto de Recursos Naturales y Agrobiologia, CSIC, Apartado 1052, 41080, Seville, Spain
    Naturwissenschaften 96:71-9. 2009
    ..The isolates reproduced some biodeterioration processes in the laboratory and revealed their biomediation in crystal formation...
  16. doi request reprint Differential microbial communities in hot spring mats from Western Thailand
    M C Portillo
    Institute of Natural Resources and Agrobiology, IRNAS CSIC, Avda Reina Mercedes 10, 41012, Sevilla, Spain
    Extremophiles 13:321-31. 2009
    ..Differently colored mat layers showed characteristic bacterial communities and the major components of the metabolically active fraction of these communities have been identified...
  17. doi request reprint Pigment profiles and bacterial communities from Thailand thermal mats
    M C Portillo
    Institute of Natural Resources and Agrobiology, IRNAS CSIC, Avda Reina Mercedes 10, 41012 Sevilla, Spain
    Antonie Van Leeuwenhoek 96:559-67. 2009
    ..Differently colored mat layers showed characteristic bacterial communities which could be discriminated from pigment profiles and molecular surveys...
  18. doi request reprint Statistical differences between relative quantitative molecular fingerprints from microbial communities
    M C Portillo
    Instituto de Recursos Naturales y Agrobiologia, CSIC, Avda Reina Mercedes 10, 41012 Sevilla, Spain
    Antonie Van Leeuwenhoek 94:157-63. 2008
    ..The method was able to discriminate among different samples based on their molecular fingerprints, and confirmed the lack of differences between aliquots from a single sample...
  19. ncbi request reprint Phyllobacterium catacumbae sp. nov., a member of the order 'Rhizobiales' isolated from Roman catacombs
    V Jurado
    Instituto de Recursos Naturales y Agrobiologia, CSIC, Apartado 1052, 41080 Sevilla, Spain
    Int J Syst Evol Microbiol 55:1487-90. 2005
    ..The species Phyllobacterium catacumbae sp. nov. is proposed. The type strain is CSC19(T) (=CECT 5680(T)=LMG 22520(T))...
  20. doi request reprint Three different phototrophic microbial communities colonizing a single natural shelter containing prehistoric paintings
    M C Portillo
    Instituto de Recursos Naturales y Agrobiologia, CSIC, Avda Reina Mercedes 10, 41012 Sevilla, Spain
    Sci Total Environ 407:4876-81. 2009
    ....
  21. doi request reprint CRISPR elements in the Thermococcales: evidence for associated horizontal gene transfer in Pyrococcus furiosus
    M C Portillo
    Institute for Natural Resources and Agrobiology, IRNAS CSIC, Sevilla, Spain
    J Appl Genet 50:421-30. 2009
    ..furiosus genome. These results represent the first evidence suggesting that repeated CRISPR elements can be involved in horizontal gene transfer and genomic differentiation of hyperthermophilic Archaea...
  22. ncbi request reprint Reclassification of Agromyces fucosus subsp. hippuratus as Agromyces hippuratus sp. nov., comb. nov. and emended description of Agromyces fucosus
    A Ortiz-Martinez
    Instituto de Recursos Naturales y Agrobiologia de Sevilla, CSIC, Apartado 1052, 41080 Sevilla, Spain
    Int J Syst Evol Microbiol 54:1553-6. 2004
    ..fucosus subsp. hippuratus as a separate species, Agromyces hippuratus sp. nov., comb. nov. The type strain is VKM Ac-1352T (= JCM 9087T). An emended description of Agromyces fucosus is given...
  23. doi request reprint Role of specific microbial communities in the bioavailability of iron in Doñana National Park
    M C Portillo
    Institute of Natural Resources and Agrobiology, CSIC, Avda Reina Mercedes 10, 41012 Sevilla, Spain
    Environ Geochem Health 30:165-70. 2008
    ..In addition, they provide significant insights into the bioavailability of these elements with potential interest in the role of microorganisms for metal sequestration in natural environments and utilization in bioremediation processes...
  24. ncbi request reprint Coronavirus derived expression systems
    L Enjuanes
    Department of Molecular and Cell Biology, Centro Nacional de Biotecnologia, CSIC, Campus Universidad Autonoma, Cantoblanco, 28049, Madrid, Spain
    J Biotechnol 88:183-204. 2001
    ..Thus, coronaviruses are promising virus vectors for vaccine development and, possibly, for gene therapy...
  25. doi request reprint Is the availability of different nutrients a critical factor for the impact of bacteria on subterraneous carbon budgets?
    M C Portillo
    Instituto de Recursos Naturales y Agrobiologia, IRNAS CSIC, Avda Reina Mercedes 10, Sevilla 41012, Spain
    Naturwissenschaften 96:1035-42. 2009
    ....
  26. ncbi request reprint Performance and microbial communities of a continuous stirred tank anaerobic reactor treating two-phases olive mill solid wastes at low organic loading rates
    B Rincon
    Instituto de la Grasa, CSIC, Avda Padre Garcia Tejero 4, 41012 Sevilla, Spain
    J Biotechnol 121:534-43. 2006
    ..Other bacterial groups detected in the bioreactor were the Actinobacteria, Bacteroidetes and Deferribacteres. Among the Archaea, the methanogen Methanosaeta concilii was the most representative species...
  27. doi request reprint Presence and potential role of thermophilic bacteria in temperate terrestrial environments
    M C Portillo
    IRNAS CSIC, Avda Reina Mercedes 10, Sevilla, 41012, Spain
    Naturwissenschaften 99:43-53. 2012
    ..These results support a potential role of thermophilic bacteria in temperate terrestrial environments by mineralizing organic sulfur and nitrogen ruled by the existence and length of warm periods...
  28. ncbi request reprint Complete genome sequence of transmissible gastroenteritis coronavirus PUR46-MAD clone and evolution of the purdue virus cluster
    Z Penzes
    , CSIC, Department of Molecular and Cell Biology, Cantoblaico, Madrid, Spain
    Virus Genes 23:105-18. 2001
    ..Comparison of the TGEV polymerase sequence with that of other RNA viruses revealed high sequence homology with the A-E domains of the palm subdomain of nucleic acid polymerases...
  29. ncbi request reprint Deterioration of building materials in Roman catacombs: the influence of visitors
    S Sanchez-Moral
    Departamento de Geologia, Museo Nacional de Ciencias Naturales CSIC, Jose Gutierrez Abascal 2, Madrid, Spain
    Sci Total Environ 349:260-76. 2005
    ..In addition, biofilm distribution was governed by the type of material to be colonised. This study shows that countermeasures are needed to prevent deterioration of hypogean environments...
  30. pmc Development of two PCR-based techniques for detecting helical and coccoid forms of Helicobacter pylori
    M Shahamat
    Center of Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore, MD 21202, USA
    J Clin Microbiol 42:3613-9. 2004
    ..pylori and closely related Helicobacter spp., the primers for sequences flanking the 16S rRNA gene can confirm the presence of H. pylori and locate the potential source of this bacterium...
  31. ncbi request reprint Anion exchanger 1 (band 3) is required to prevent erythrocyte membrane surface loss but not to form the membrane skeleton
    L L Peters
    The Jackson Laboratory, Bar Harbor, Maine 04609, USA
    Cell 86:917-27. 1996
    ..We postulate that stabilization is achieved through AE1-lipid interactions and that loss of these interactions is a key pathogenic event in hereditary spherocytosis...