Francisco Garcia-del Portillo

Summary

Country: Spain

Publications

  1. pmc A novel antisense RNA from the Salmonella virulence plasmid pSLT expressed by non-growing bacteria inside eukaryotic cells
    Jesús Gonzalo-Asensio
    Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Científicas CNB CSIC, Madrid, Spain
    PLoS ONE 8:e77939. 2013
  2. pmc A new view to intracellular pathogens and host responses in the South of Spain
    Francisco Garcia-del Portillo
    Centro Nacional de Biotecnologia CNB CSIC, Madrid, Spain
    EMBO Mol Med 4:160-4. 2012
  3. doi request reprint Heterogeneity in tissue culture infection models: a source of novel host-pathogen interactions?
    Francisco Garcia-del Portillo
    Departamento de Biotecnologia Microbiana, Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Cientificas, 28049 Madrid, Spain
    Microbes Infect 10:1063-6. 2008
  4. pmc Genome expression analysis of nonproliferating intracellular Salmonella enterica serovar Typhimurium unravels an acid pH-dependent PhoP-PhoQ response essential for dormancy
    Cristina Núñez-Hernández
    Departamento de Biotecnologia Microbiana, Centro Nacional de Biotecnología Consejo Superior de Investigaciones Científicas, Madrid, Spain
    Infect Immun 81:154-65. 2013
  5. doi request reprint Occurrence of mutations impairing sigma factor B (SigB) function upon inactivation of Listeria monocytogenes genes encoding surface proteins
    Juan J Quereda
    Centro Nacional de Biotecnología Consejo Superior de Investigaciones Científicas CNB CSIC, 28049 Madrid, Spain
    Microbiology 159:1328-39. 2013
  6. ncbi request reprint Analysis of the Listeria cell wall proteome by two-dimensional nanoliquid chromatography coupled to mass spectrometry
    Enrique Calvo
    Unidad de Proteómica, Fundación Centro Nacional de Investigaciones Cardiovasculares, Tres Cantos, Madrid, Spain
    Proteomics 5:433-43. 2005
  7. pmc Dormant intracellular Salmonella enterica serovar Typhimurium discriminates among Salmonella pathogenicity island 2 effectors to persist inside fibroblasts
    Cristina Núñez-Hernández
    Departamento de Biotecnologia Microbiana, Centro Nacional de Biotecnología Consejo Superior de Investigaciones Científicas CNB CSIC, Madrid, Spain
    Infect Immun 82:221-32. 2014
  8. ncbi request reprint Repression of the RcsC-YojN-RcsB phosphorelay by the IgaA protein is a requisite for Salmonella virulence
    Gustavo Domínguez-Bernal
    Departamento de Biotecnologia Microbiana, Centro Nacional de Biotecnologia Consejo Superior de Investigaciones Cientificas CSIC, Campus de Cantoblanco, 28049 Madrid, Spain
    Mol Microbiol 53:1437-49. 2004
  9. pmc Association of ActA to peptidoglycan revealed by cell wall proteomics of intracellular Listeria monocytogenes
    Francisco Garcia-del Portillo
    Departamento de Biotecnologia Microbiana, Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Cientificas, 28049 Madrid, Spain
    J Biol Chem 286:34675-89. 2011
  10. ncbi request reprint Inverse regulation in the metabolic genes pckA and metE revealed by proteomic analysis of the Salmonella RcsCDB regulon
    Alberto Paradela
    Laboratorio de Proteómica, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas CSIC, Madrid, Spain
    J Proteome Res 10:3386-98. 2011

Collaborators

Detail Information

Publications22

  1. pmc A novel antisense RNA from the Salmonella virulence plasmid pSLT expressed by non-growing bacteria inside eukaryotic cells
    Jesús Gonzalo-Asensio
    Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Científicas CNB CSIC, Madrid, Spain
    PLoS ONE 8:e77939. 2013
    ..As exemplified by IesR-1, some of these sRNAs may contribute to diminish the relative levels of proteins, such as PSLT047, which are probably dispensable for the intracellular lifestyle. ..
  2. pmc A new view to intracellular pathogens and host responses in the South of Spain
    Francisco Garcia-del Portillo
    Centro Nacional de Biotecnologia CNB CSIC, Madrid, Spain
    EMBO Mol Med 4:160-4. 2012
    ....
  3. doi request reprint Heterogeneity in tissue culture infection models: a source of novel host-pathogen interactions?
    Francisco Garcia-del Portillo
    Departamento de Biotecnologia Microbiana, Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Cientificas, 28049 Madrid, Spain
    Microbes Infect 10:1063-6. 2008
    ..Despite its obvious impact in data interpretation, the basis of this heterogeneity remains in most cases unknown. Addressing this issue is encouraging since may contribute to uncover novel interactions in the host-pathogen scenario...
  4. pmc Genome expression analysis of nonproliferating intracellular Salmonella enterica serovar Typhimurium unravels an acid pH-dependent PhoP-PhoQ response essential for dormancy
    Cristina Núñez-Hernández
    Departamento de Biotecnologia Microbiana, Centro Nacional de Biotecnología Consejo Superior de Investigaciones Científicas, Madrid, Spain
    Infect Immun 81:154-65. 2013
    ..Typhimurium restrains intracellular growth in vivo and support a model in which dormant intracellular bacteria could sense vacuolar acidification to stimulate the PhoP-PhoQ system for preventing intracellular overgrowth...
  5. doi request reprint Occurrence of mutations impairing sigma factor B (SigB) function upon inactivation of Listeria monocytogenes genes encoding surface proteins
    Juan J Quereda
    Centro Nacional de Biotecnología Consejo Superior de Investigaciones Científicas CNB CSIC, 28049 Madrid, Spain
    Microbiology 159:1328-39. 2013
    ..monocytogenes prior to any subsequent phenotypic analysis. This test may be even more justified when generating deletions affecting cell envelope components. ..
  6. ncbi request reprint Analysis of the Listeria cell wall proteome by two-dimensional nanoliquid chromatography coupled to mass spectrometry
    Enrique Calvo
    Unidad de Proteómica, Fundación Centro Nacional de Investigaciones Cardiovasculares, Tres Cantos, Madrid, Spain
    Proteomics 5:433-43. 2005
    ..This information will be useful to study novel protein-peptidoglycan associations and to rapidly identify new targets in the surface of important bacterial pathogens...
  7. pmc Dormant intracellular Salmonella enterica serovar Typhimurium discriminates among Salmonella pathogenicity island 2 effectors to persist inside fibroblasts
    Cristina Núñez-Hernández
    Departamento de Biotecnologia Microbiana, Centro Nacional de Biotecnología Consejo Superior de Investigaciones Científicas CNB CSIC, Madrid, Spain
    Infect Immun 82:221-32. 2014
    ..We propose that the S. enterica serovar Typhimurium nonproliferative intracellular lifestyle is sustained by selection of SPI-2 effectors that are produced in tightly defined amounts and delivered to phagosome-confined locations. ..
  8. ncbi request reprint Repression of the RcsC-YojN-RcsB phosphorelay by the IgaA protein is a requisite for Salmonella virulence
    Gustavo Domínguez-Bernal
    Departamento de Biotecnologia Microbiana, Centro Nacional de Biotecnologia Consejo Superior de Investigaciones Cientificas CSIC, Campus de Cantoblanco, 28049 Madrid, Spain
    Mol Microbiol 53:1437-49. 2004
    ..To our knowledge, IgaA exemplifies the first protein that contributes to virulence by exclusively acting as a negative regulator upon host colonization...
  9. pmc Association of ActA to peptidoglycan revealed by cell wall proteomics of intracellular Listeria monocytogenes
    Francisco Garcia-del Portillo
    Departamento de Biotecnologia Microbiana, Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Cientificas, 28049 Madrid, Spain
    J Biol Chem 286:34675-89. 2011
    ..Some of these changes include alterations in the relative amount and the mode of association of certain surface proteins...
  10. ncbi request reprint Inverse regulation in the metabolic genes pckA and metE revealed by proteomic analysis of the Salmonella RcsCDB regulon
    Alberto Paradela
    Laboratorio de Proteómica, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas CSIC, Madrid, Spain
    J Proteome Res 10:3386-98. 2011
    ..Collectively, these findings provide new insights into post-transcriptional regulatory mechanisms involving the RcsCDB system and its control over metabolic functions...
  11. ncbi request reprint Analysis of Salmonella invasion protein-peptidoglycan interactions
    M Graciela Pucciarelli
    Department of Microbial Biotechnology, Centro Nacional de Biotecnologia CSIC, 28049 Madrid, Spain
    Methods Enzymol 358:393-409. 2002
  12. ncbi request reprint Envelope instability in DNA adenine methylase mutants of Salmonella enterica
    M Graciela Pucciarelli
    Departamento de Biotecnologia Microbiana, Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Cientificas CSIC, Campus de Cantoblanco, 28049 Madrid, Spain
    Microbiology 148:1171-82. 2002
    ..Altogether, these results indicate that, besides its role in modulating the secretion of effectors by the SPI-1-encoded type III apparatus, Dam methylation controls cell envelope integrity in S. enterica...
  13. ncbi request reprint New concepts in Salmonella virulence: the importance of reducing the intracellular growth rate in the host
    Alberto Tierrez
    Departamento de Biotecnologia Microbiana, Centro Nacional de Biotecnologia Consejo Superior de Investigaciones Cientificas CSIC, Darwin 3, 28049 Madrid, Spain
    Cell Microbiol 7:901-9. 2005
    ..These responses could play a critical role in modulating the delicate balance between disease and persistence...
  14. ncbi request reprint Protein-peptidoglycan interactions modulate the assembly of the needle complex in the Salmonella invasion-associated type III secretion system
    M Graciela Pucciarelli
    Departamento de Biotecnologia Microbiana, Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Cientificas CSIC, Campus de Cantoblanco, 28049 Madrid, Spain
    Mol Microbiol 48:573-85. 2003
    ..In addition, we demonstrate that these protein-peptidoglycan interactions are critical for an efficient and correct assembly of this specialized organelle...
  15. doi request reprint Dynamics of Salmonella small RNA expression in non-growing bacteria located inside eukaryotic cells
    Alvaro D Ortega
    Departamento de Biotecnologia Microbiana, Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Cientificas CSIC, Madrid, Spain
    RNA Biol 9:469-88. 2012
    ..Typhimurium sRNA expression in intracellular bacteria and provide the first insights into sRNAs that may direct pathogen adaptation to a non-proliferative state inside the host cell...
  16. pmc Increased excision of the Salmonella prophage ST64B caused by a deficiency in Dam methylase
    Ana Alonso
    Departamento Biotecnología Microbiana, Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Cientificas CSIC, Madrid, Spain
    J Bacteriol 187:7901-11. 2005
    ..The exacerbated excision of ST64B does not however contribute to the incapacity of dam mutants to cause disease...
  17. ncbi request reprint The Listeria monocytogenes LPXTG surface protein Lmo1413 is an invasin with capacity to bind mucin
    Javier F Mariscotti
    Departamento de Biotecnologia Microbiana, Centro Nacional de Biotecnología Consejo Superior de Investigaciones Científicas CNB CSIC, Darwin 3, 28049 Madrid, Spain
    Int J Med Microbiol 304:393-404. 2014
    ..Taken together, these data indicate that Lmo1413, which we rename LmiA, for Listeria-mucin-binding invasin-A, may promote interaction of bacteria with adhesive host protective components and, in this manner, facilitate bacterial entry. ..
  18. pmc Salmonella enterica serovar typhimurium invades fibroblasts by multiple routes differing from the entry into epithelial cells
    Ana Aiastui
    Departamento de Biotecnologia Microbiana, Centro Nacional de Biotecnología Consejo Superior de Investigaciones Científicas, Darwin 3, 28049 Madrid, Spain
    Infect Immun 78:2700-13. 2010
    ..Collectively, these data reveal a marked diversity in the modes used by S. Typhimurium to enter into fibroblasts...
  19. ncbi request reprint Identification of substrates of the Listeria monocytogenes sortases A and B by a non-gel proteomic analysis
    M Graciela Pucciarelli
    Departamento de Biotecnologia Microbiana, Centro Nacional de Biotecnologia Consejo Superior de Investigaciones Cientificas CSIC, 28049 Madrid, Spain
    Proteomics 5:4808-17. 2005
    ..Taken together, these data demonstrate that non-gel proteomics is a powerful technique to rapidly identify sortase substrates and to gain insights on potential sorting motifs...
  20. pmc The Salmonella membrane protein IgaA modulates the activity of the RcsC-YojN-RcsB and PhoP-PhoQ regulons
    Alberto Tierrez
    Departamento de Biotecnologia Microbiana, Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Cientificas, Campus de Cantoblanco, Madrid, Spain
    J Bacteriol 186:7481-9. 2004
    ..Consistent with the phenotypes observed in fibroblast cells, IgaA is predicted to favor expression of the entire PhoP-PhoQ regulon based on its repression of the RcsC-YojN-RcsB phosphorelay...
  21. ncbi request reprint Hijacking of eukaryotic functions by intracellular bacterial pathogens
    Ana Alonso
    Department of Microbial Biotechnology, National Center for Biotechnology, CSIC, Madrid, Spain
    Int Microbiol 7:181-91. 2004
    ..These new technologies are yielding valuable clues as to how these specialized bacterial pathogens manipulate the mammalian host cell...
  22. doi request reprint Evaluation of isotope-coded protein labeling (ICPL) in the quantitative analysis of complex proteomes
    Alberto Paradela
    Laboratório de Proteômica, Centro Nacional de Biotecnologia, Consejo Superior de Investigaciones Cientificas CSIC, Madrid, Spain
    Talanta 80:1496-502. 2010
    ..In addition, our results suggest that labeling at the peptide level instead of following the standard ICPL workflow should increase both the number of proteins quantified and the reliability of the quantification...