Ricardo Escalante

Summary

Country: Spain

Publications

  1. ncbi request reprint Autophagy dysfunction and ubiquitin-positive protein aggregates in Dictyostelium cells lacking Vmp1
    Javier Calvo-Garrido
    Instituto de Investigaciones Biomedicas Alberto Sols, CSIC UAM, Madrid, Spain
    Autophagy 6:100-9. 2010
  2. ncbi request reprint A Serum Response Factor homolog is required for spore differentiation in Dictyostelium
    R Escalante
    Instituto de Investigaciones Biomedicas del Consejo Superior de Investigaciones Cientificas, c Arturo Duperier 4, Spain
    Development 125:3801-8. 1998
  3. ncbi request reprint Dictyostelium discoideum: a model system for differentiation and patterning
    R Escalante
    Instituto de Investigaciones Biomedicas CSIC UAM, Madrid, Spain
    Int J Dev Biol 44:819-35. 2000
  4. ncbi request reprint The MADS-box gene srfA is expressed in a complex pattern under the control of alternative promoters and is essential for different aspects of Dictyostelium development
    R Escalante
    Instituto de Investigaciones Biomedicas, C S I C U A M, c Arturo Duperier 4, 28029 Madrid, Spain
    Dev Biol 235:314-29. 2001
  5. ncbi request reprint cAMP and DIF-1 repress the expression of the Dictyostelium MADS-box gene srfA at early stages of development
    R Escalante
    Instituto de Investigaciones Biomedicas del Consejo Superior de Investigaciones Cientificas, C Arturo Duperier, 4, 28029 Madrid, Spain
    Biochem Biophys Res Commun 285:820-4. 2001
  6. ncbi request reprint Regulated expression of the MADS-box transcription factor SrfA mediates activation of gene expression by protein kinase A during Dictyostelium sporulation
    Ricardo Escalante
    Instituto de Investigaciones Biomedicas, CSIC UAM, c Arturo Duperier 4, 28029 Madrid, Spain
    Mech Dev 117:201-8. 2002
  7. pmc Dictyostelium discoideum developmentally regulated genes whose expression is dependent on MADS box transcription factor SrfA
    Ricardo Escalante
    Instituto de Investigaciones Biomedicas, CSIC UAM, 28029 Madrid, Spain
    Eukaryot Cell 2:1327-35. 2003
  8. pmc Structural and functional studies of a family of Dictyostelium discoideum developmentally regulated, prestalk genes coding for small proteins
    Juan J Vicente
    Instituto de Investigaciones Biomedicas, CSIC UAM, C Arturo Duperier, 4, 28029, Madrid, Spain
    BMC Microbiol 8:1. 2008
  9. pmc Ndufaf5 deficiency in the Dictyostelium model: new roles in autophagy and development
    Sergio Carilla-Latorre
    Instituto de Investigaciones Biomedicas Alberto Sols, Consejo Superior de Investigaciones Cientificas Universidad Autonoma de Madrid, 28029 Madrid, Spain
    Mol Biol Cell 24:1519-28. 2013
  10. pmc Vacuole membrane protein 1 is an endoplasmic reticulum protein required for organelle biogenesis, protein secretion, and development
    Javier Calvo-Garrido
    Instituto de Investigaciones Biomedicas Alberto Sols, Consejo Superior de Investigaciones Cientificas and Universidad Autonoma de Madrid, 28029 Madrid, Spain
    Mol Biol Cell 19:3442-53. 2008

Collaborators

Detail Information

Publications24

  1. ncbi request reprint Autophagy dysfunction and ubiquitin-positive protein aggregates in Dictyostelium cells lacking Vmp1
    Javier Calvo-Garrido
    Instituto de Investigaciones Biomedicas Alberto Sols, CSIC UAM, Madrid, Spain
    Autophagy 6:100-9. 2010
    ..Our results suggest that Vmp1 is required for the clearance of these ubiquitinated protein aggregates through autophagy and highlight a potential role for Vmp1 in protein-aggregation diseases...
  2. ncbi request reprint A Serum Response Factor homolog is required for spore differentiation in Dictyostelium
    R Escalante
    Instituto de Investigaciones Biomedicas del Consejo Superior de Investigaciones Cientificas, c Arturo Duperier 4, Spain
    Development 125:3801-8. 1998
    ....
  3. ncbi request reprint Dictyostelium discoideum: a model system for differentiation and patterning
    R Escalante
    Instituto de Investigaciones Biomedicas CSIC UAM, Madrid, Spain
    Int J Dev Biol 44:819-35. 2000
    ....
  4. ncbi request reprint The MADS-box gene srfA is expressed in a complex pattern under the control of alternative promoters and is essential for different aspects of Dictyostelium development
    R Escalante
    Instituto de Investigaciones Biomedicas, C S I C U A M, c Arturo Duperier 4, 28029 Madrid, Spain
    Dev Biol 235:314-29. 2001
    ..Our results demonstrate that the correct spatial and temporal pattern of expression of srfA is essential for the different functions that this transcription factor plays in development...
  5. ncbi request reprint cAMP and DIF-1 repress the expression of the Dictyostelium MADS-box gene srfA at early stages of development
    R Escalante
    Instituto de Investigaciones Biomedicas del Consejo Superior de Investigaciones Cientificas, C Arturo Duperier, 4, 28029 Madrid, Spain
    Biochem Biophys Res Commun 285:820-4. 2001
    ..Addition of other extracellular compounds such as ammonia, adenosine, SDF-1, and SDF-2 had no effect. srfA promoter P2, specifically induced during slug migration, was responsible for this regulation by extracellular compounds...
  6. ncbi request reprint Regulated expression of the MADS-box transcription factor SrfA mediates activation of gene expression by protein kinase A during Dictyostelium sporulation
    Ricardo Escalante
    Instituto de Investigaciones Biomedicas, CSIC UAM, c Arturo Duperier 4, 28029 Madrid, Spain
    Mech Dev 117:201-8. 2002
    ..The correct temporal and spatial pattern of srfA expression appears to be part of a mechanism that ensures the adequate coordination of gene expression and morphogenesis...
  7. pmc Dictyostelium discoideum developmentally regulated genes whose expression is dependent on MADS box transcription factor SrfA
    Ricardo Escalante
    Instituto de Investigaciones Biomedicas, CSIC UAM, 28029 Madrid, Spain
    Eukaryot Cell 2:1327-35. 2003
    ..Strains in which either sigA, sigB, or sigD is disrupted were isolated and found to form spores that are not detectably different from those of wild-type strains...
  8. pmc Structural and functional studies of a family of Dictyostelium discoideum developmentally regulated, prestalk genes coding for small proteins
    Juan J Vicente
    Instituto de Investigaciones Biomedicas, CSIC UAM, C Arturo Duperier, 4, 28029, Madrid, Spain
    BMC Microbiol 8:1. 2008
    ..The MADS-box transcription factor SrfA is required for several stages of development, including slug migration and spore terminal differentiation...
  9. pmc Ndufaf5 deficiency in the Dictyostelium model: new roles in autophagy and development
    Sergio Carilla-Latorre
    Instituto de Investigaciones Biomedicas Alberto Sols, Consejo Superior de Investigaciones Cientificas Universidad Autonoma de Madrid, 28029 Madrid, Spain
    Mol Biol Cell 24:1519-28. 2013
    ..This phenomenon was also observed in a Dictyostelium mutant lacking MidA (C2orf56/PRO1853/Ndufaf7), another CI assembly factor, suggesting that autophagy activation might be a common feature in mitochondrial CI dysfunction...
  10. pmc Vacuole membrane protein 1 is an endoplasmic reticulum protein required for organelle biogenesis, protein secretion, and development
    Javier Calvo-Garrido
    Instituto de Investigaciones Biomedicas Alberto Sols, Consejo Superior de Investigaciones Cientificas and Universidad Autonoma de Madrid, 28029 Madrid, Spain
    Mol Biol Cell 19:3442-53. 2008
    ..In addition to these defects observed at the vegetative stage, the onset of multicellular development and early developmental gene expression are also compromised...
  11. doi request reprint Monitoring autophagy in Dictyostelium
    Ana Mesquita
    Instituto de Investigaciones Biomedicas Alberto Sols CSIC UAM, Madrid, Spain
    Methods Mol Biol 983:461-70. 2013
    ..The first one is a proteolytic cleavage assay of cytosolic markers such as GFP-PgkA and the second the visualization of the RFP-GFP-Atg8 marker by confocal microscopy...
  12. pmc Identification of genes dependent on the MADS box transcription factor SrfA in Dictyostelium discoideum development
    Ricardo Escalante
    Instituto de Investigaciones Biomedicas CSIC UAM, 28029 Madrid, Spain
    Eukaryot Cell 3:564-6. 2004
    ..In wild-type cells, all of these genes are induced late in development. At least four of them are necessary for proper spore differentiation, stability, and/or germination...
  13. pmc Optimization of a large-scale gene disruption protocol in Dictyostelium and analysis of conserved genes of unknown function
    Patricia Torija
    Instituto de Investigaciones Biomédicas Alberto Sols C S I C U A M, Calle Arturo Duperier 4, 28029 Madrid, Spain
    BMC Microbiol 6:75. 2006
    ....
  14. ncbi request reprint The MADS-box transcription factor SrfA is required for actin cytoskeleton organization and spore coat stability during Dictyostelium sporulation
    Ricardo Escalante
    Instituto de Investigaciones Biomedicas CSIC UAM, Gene Expression, Arturo Duperier, 4, 28029 Madrid, Spain
    Mech Dev 121:51-6. 2004
    ..Taken together, these results suggest that SrfA is involved in late events of spore maturation necessary for spore stability...
  15. doi request reprint MidA is a putative methyltransferase that is required for mitochondrial complex I function
    Sergio Carilla-Latorre
    Instituto de Investigaciones Biomedicas Alberto Sols CSIC UAM, Arturo Duperier 4, 28029 Madrid, Spain
    J Cell Sci 123:1674-83. 2010
    ..We found that these aspects of the phenotype are mediated by a chronic activation of AMPK, revealing a possible role of AMPK signaling in complex I cytopathology...
  16. doi request reprint A proteolytic cleavage assay to monitor autophagy in Dictyostelium discoideum
    Javier Calvo-Garrido
    Instituto de Investigaciones Biomedicas Alberto Sols, Consejo Superior de Investigaciones Cientificas Universidad Autonoma de Madrid, Madrid, Spain
    Autophagy 7:1063-8. 2011
    ..Moreover, treatment with NH4Cl also facilitates the assessment of autophagic flux by confocal microscopy using the marker RFP-GFP-Atg8...
  17. ncbi request reprint Functional genomics in Dictyostelium: MidA, a new conserved protein, is required for mitochondrial function and development
    Patricia Torija
    Instituto de Investigaciones Biomédicas Alberto Sols C S I C U A M, Calle Arturo Duperier 4, 28029 Madrid, Spain
    J Cell Sci 119:1154-64. 2006
    ..This work exemplifies the potential of Dictyostelium as a model system for functional genomic studies...
  18. ncbi request reprint Vacuole membrane protein 1, autophagy and much more
    Javier Calvo-Garrido
    Instituto de Investigaciones Biomedicas Alberto Sols, Consejo Superior de Investigaciones Cientificas Universidad Autonoma de Madrid, Madrid, Spain
    Autophagy 4:835-7. 2008
    ..We are taking the first steps in understanding the function of this fascinating protein and recent studies have brought us more questions than answers about its basic function and its role in human pathology...
  19. ncbi request reprint Autophagy in Dictyostelium: genes and pathways, cell death and infection
    Javier Calvo-Garrido
    Instituto de Investigaciones Biomedicas Alberto Sols CSIC UAM, Arturo Duperier 4, Madrid, Spain
    Autophagy 6:686-701. 2010
    ..This review focuses on the identification and annotation of the putative Dictyostelium autophagy genes and on the role of autophagy in development, cell death and infection by bacterial pathogens...
  20. pmc Dictyostelium transcriptional responses to Pseudomonas aeruginosa: common and specific effects from PAO1 and PA14 strains
    Sergio Carilla-Latorre
    Instituto de Investigaciones Biomedicas Alberto Sols, Universidad Autónoma de Madrid Consejo Superior de Investigaciones Científicas, Madrid, Spain
    BMC Microbiol 8:109. 2008
    ..aeruginosa and other pathogens, we used available genomic tools in this model system to study the transcriptional host response to P. aeruginosa infection...
  21. ncbi request reprint Investigating gene expression: in situ hybridization and reporter genes
    Ricardo Escalante
    Instituto de Investigaciones Biomedicas, CSIC UAM, Madrid, Spain
    Methods Mol Biol 346:247-60. 2006
    ..Generally used beta-galactosidase detection methods are described...
  22. pmc Role of SP65 in assembly of the Dictyostelium discoideum spore coat
    Talibah Metcalf
    Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
    Eukaryot Cell 6:1137-49. 2007
    ....
  23. ncbi request reprint Overexpression of the multidrug efflux pump SmeDEF impairs Stenotrophomonas maltophilia physiology
    Ana Alonso
    Departamento de Biotecnologia Microbiana, Centro Nacional de Biotecnologia, CSIC, Campus Universidad Autonoma de Madrid, Cantoblanco, 28049 Madrid
    J Antimicrob Chemother 53:432-4. 2004
    ..SmeDEF confers resistance to antibiotics belonging to different structural families, and its overexpression is associated with an antibiotic resistance phenotype in clinical isolates of S. maltophilia...
  24. pmc Molecular systematics of dictyostelids: 5.8S ribosomal DNA and internal transcribed spacer region analyses
    Maria Romeralo
    Real Jardín Botánico CSIC, Plaza de Murillo 2, 28014 Madrid, Spain
    Eukaryot Cell 6:110-6. 2007
    ..The nucleotide sequences of the ribosomal internal transcribed spacers and the 5.8S ribosomal DNA gene have been determined for 28 isolates, and their utility to discriminate between different species and genera has been shown...