E Domingo

Summary

Country: Spain

Publications

  1. ncbi request reprint Viruses as quasispecies: biological implications
    E Domingo
    Centro de Biologia Molecular, Severo Ochoa, CSIC UAM, Universidad Autonoma de Madrid, Cantoblanco, 28049 Madrid, Spain
    Curr Top Microbiol Immunol 299:51-82. 2006
  2. pmc Potential benefits of sequential inhibitor-mutagen treatments of RNA virus infections
    Celia Perales
    Departamento de Virología y Microbiología, Centro de Biologia Molecular Severo Ochoa CSIC UAM, Consejo Superior de Investigaciones Cientificas CSIC, Madrid, Spain
    PLoS Pathog 5:e1000658. 2009
  3. pmc Counteracting quasispecies adaptability: extinction of a ribavirin-resistant virus mutant by an alternative mutagenic treatment
    Celia Perales
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, Consejo Superior de Investigaciones Cientificas CSIC, Campus de Cantoblanco, Madrid, Spain
    PLoS ONE 4:e5554. 2009
  4. pmc Viral genome segmentation can result from a trade-off between genetic content and particle stability
    Samuel Ojosnegros
    Centro de Biologia Molecular Severo Ochoa, CSIC UAM, Madrid, Spain
    PLoS Genet 7:e1001344. 2011
  5. pmc Mutagenesis-mediated virus extinction: virus-dependent effect of viral load on sensitivity to lethal defection
    Hector Moreno
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, Cantoblanco, Madrid, Spain
    PLoS ONE 7:e32550. 2012
  6. pmc A multi-step process of viral adaptation to a mutagenic nucleoside analogue by modulation of transition types leads to extinction-escape
    Rubén Agudo
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, Cantoblanco, Madrid, Spain
    PLoS Pathog 6:e1001072. 2010
  7. pmc Extinction of hepatitis C virus by ribavirin in hepatoma cells involves lethal mutagenesis
    Ana M Ortega-Prieto
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, Consejo Superior de Investigaciones Cientificas CSIC, Campus de Cantoblanco, Madrid, Spain
    PLoS ONE 8:e71039. 2013
  8. pmc Arenaviruses and lethal mutagenesis. Prospects for new ribavirin-based interventions
    Hector Moreno
    Centro de Biologia Molecular Severo Ochoa, Campus de Cantoblanco 28049, Madrid, Spain
    Viruses 4:2786-805. 2012
  9. pmc Viral quasispecies evolution
    Esteban Domingo
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, C Nicolás Cabrera, Universidad Autonoma de Madrid, Cantoblanco, Madrid, Spain
    Microbiol Mol Biol Rev 76:159-216. 2012
  10. pmc Microarray-based identification of antigenic variants of foot-and-mouth disease virus: a bioinformatics quality assessment
    Veronica Martin
    Centro de Biologia Molecular Severo Ochoa, Universidad Autonoma de Madrid, Cantoblanco, 28049, Madrid, Spain
    BMC Genomics 7:117. 2006

Collaborators

Detail Information

Publications141 found, 100 shown here

  1. ncbi request reprint Viruses as quasispecies: biological implications
    E Domingo
    Centro de Biologia Molecular, Severo Ochoa, CSIC UAM, Universidad Autonoma de Madrid, Cantoblanco, 28049 Madrid, Spain
    Curr Top Microbiol Immunol 299:51-82. 2006
    ..Also, mutant spectra are the target on which selection and random drift act to shape the long-term evolution of viruses. The biological relevance of mutant spectra is the central topic of this chapter...
  2. pmc Potential benefits of sequential inhibitor-mutagen treatments of RNA virus infections
    Celia Perales
    Departamento de Virología y Microbiología, Centro de Biologia Molecular Severo Ochoa CSIC UAM, Consejo Superior de Investigaciones Cientificas CSIC, Madrid, Spain
    PLoS Pathog 5:e1000658. 2009
    ..The results may impact lethal mutagenesis-based protocols, as well as current antiviral therapies involving ribavirin...
  3. pmc Counteracting quasispecies adaptability: extinction of a ribavirin-resistant virus mutant by an alternative mutagenic treatment
    Celia Perales
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, Consejo Superior de Investigaciones Cientificas CSIC, Campus de Cantoblanco, Madrid, Spain
    PLoS ONE 4:e5554. 2009
    ..Previously, we characterized a mutant of foot-and-mouth disease virus that included in its RNA-dependent RNA polymerase replacement M296I that decreased the sensitivity of the virus to the mutagenic nucleoside analogue ribavirin...
  4. pmc Viral genome segmentation can result from a trade-off between genetic content and particle stability
    Samuel Ojosnegros
    Centro de Biologia Molecular Severo Ochoa, CSIC UAM, Madrid, Spain
    PLoS Genet 7:e1001344. 2011
    ..Genome segmentation allows maximizing the genetic content without the detrimental effect in stability derived from incresing genome length...
  5. pmc Mutagenesis-mediated virus extinction: virus-dependent effect of viral load on sensitivity to lethal defection
    Hector Moreno
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, Cantoblanco, Madrid, Spain
    PLoS ONE 7:e32550. 2012
    ..Viral load and virus fitness are known to influence virus extinction. Here we examine the effect or the multiplicity of infection (MOI) on progeny production of several RNA viruses under enhanced mutagenesis...
  6. pmc A multi-step process of viral adaptation to a mutagenic nucleoside analogue by modulation of transition types leads to extinction-escape
    Rubén Agudo
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, Cantoblanco, Madrid, Spain
    PLoS Pathog 6:e1001072. 2010
    ....
  7. pmc Extinction of hepatitis C virus by ribavirin in hepatoma cells involves lethal mutagenesis
    Ana M Ortega-Prieto
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, Consejo Superior de Investigaciones Cientificas CSIC, Campus de Cantoblanco, Madrid, Spain
    PLoS ONE 8:e71039. 2013
    ..We discuss some possibilities to explain disparate results on ribavirin mutagenesis of HCV. ..
  8. pmc Arenaviruses and lethal mutagenesis. Prospects for new ribavirin-based interventions
    Hector Moreno
    Centro de Biologia Molecular Severo Ochoa, Campus de Cantoblanco 28049, Madrid, Spain
    Viruses 4:2786-805. 2012
    ..Given recent progress in the development of new inhibitors of arenavirus replication, our results suggest new options of ribavirin-based anti-arenavirus treatments...
  9. pmc Viral quasispecies evolution
    Esteban Domingo
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, C Nicolás Cabrera, Universidad Autonoma de Madrid, Cantoblanco, Madrid, Spain
    Microbiol Mol Biol Rev 76:159-216. 2012
    ..Finally, extensions of quasispecies to nonviral systems are briefly mentioned to emphasize the broad applicability of quasispecies theory...
  10. pmc Microarray-based identification of antigenic variants of foot-and-mouth disease virus: a bioinformatics quality assessment
    Veronica Martin
    Centro de Biologia Molecular Severo Ochoa, Universidad Autonoma de Madrid, Cantoblanco, 28049, Madrid, Spain
    BMC Genomics 7:117. 2006
    ..These results encourage the development of new oligonucleotide microarrays to probe the fine genetic and antigenic composition of FMDV for diagnosis, vaccine design, and to gain insight into the molecular epidemiology of this pathogen...
  11. pmc Topology of evolving, mutagenized viral populations: quasispecies expansion, compression, and operation of negative selection
    Samuel Ojosnegros
    Centro de Biologia Molecular Severo Ochoa, UAM CSIC, Campus de Cantoblanco, 28049, Madrid, Spain
    BMC Evol Biol 8:207. 2008
    ....
  12. ncbi request reprint Virus population dynamics, fitness variations and the control of viral disease: an update
    E Domingo
    Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientificas, Universidad Autonoma de Madrid, Cantoblanco, Spain
    Prog Drug Res 57:77-115. 2001
    ..Future trends in multiple target antiviral therapy and new approaches based on virus entry into error catastrophe (extinction mutagenesis) are discussed...
  13. ncbi request reprint Quasispecies and the implications for virus persistence and escape
    E Domingo
    Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientificas, Universidad Autonoma de Madrid, Cantoblanco, Spain
    Clin Diagn Virol 10:97-101. 1998
    ..In striking similarity with the proposals for such early replicons, present-day RNA viruses consist of complex distributions of nonidentical but closely related genomes termed quasispecies...
  14. ncbi request reprint Future prospects for the treatment of rapidly evolving viral pathogens: insights from evolutionary biology
    Esteban Domingo
    Universidad Autonoma de Madrid, Centro de Biologia Molecular Severo Ochoa CSIC UAM, Madrid, Spain
    Expert Opin Biol Ther 8:1455-60. 2008
    ..An alternative design, termed lethal mutagenesis or virus entry into error catastrophe is presented. It exploits the high error rates inherent in RNA virus replication, to provoke virus extinction through excess of mutations...
  15. ncbi request reprint Coxsackieviruses and quasispecies theory: evolution of enteroviruses
    E Domingo
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, Universidad Autonoma de Madrid, Cantoblanco, 28049 Madrid, Spain
    Curr Top Microbiol Immunol 323:3-32. 2008
    ....
  16. ncbi request reprint Emergence and selection of RNA virus variants: memory and extinction
    Esteban Domingo
    Centro de Biologia Molecular, Severo Ochoa CSIC UAM, Universidad Autonoma de Madrid, Cantoblanco, Spain
    Virus Res 82:39-44. 2002
    ..These observations, based on the theoretical prediction of the existence of a copying error-threshold for maintenance of genetic information, may contribute to the development of a new antiviral strategy...
  17. ncbi request reprint Quasispecies dynamics and RNA virus extinction
    Esteban Domingo
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, Consejo Superior de Investigaciones Cientificas, Universidad Autonoma de Madrid, Cantoblanco, 28049 Madrid, Spain
    Virus Res 107:129-39. 2005
    ..The results suggest procedures to master key parameters to develop effective antiviral strategies based on virus entry into error catastrophe...
  18. ncbi request reprint Evolution of foot-and-mouth disease virus
    Esteban Domingo
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, Universidad Autonoma de Madrid, Cantoblanco, 28049 Madrid, Spain
    Virus Res 91:47-63. 2003
    ..Studies with FMDV have contributed to the understanding of quasispecies dynamics and some of its biological implications...
  19. ncbi request reprint Quasispecies and the development of new antiviral strategies
    Esteban Domingo
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, Universidad Autonoma de Madrid, Cantoblanco, 28049 Madrid, Spain
    Prog Drug Res 60:133-58. 2003
    ....
  20. ncbi request reprint Quasispecies and its impact on viral hepatitis
    Esteban Domingo
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, Universidad Autonoma de Madrid, Cantoblanco, 28049 Madrid, Spain
    Virus Res 127:131-50. 2007
    ....
  21. pmc Paradoxical interplay of viral and cellular functions
    Esteban Domingo
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, C Nicolás Cabrera, 1, Cantoblanco, Madrid 28049, Spain
    Viruses 3:272-7. 2011
    ....
  22. ncbi request reprint Viruses at the edge of adaptation
    E Domingo
    Centro de Biologia Molecular Severo Ochoa, CSIC UAM Universidad Autonoma de Madrid, Cantoblanco, Madrid, 28049, Spain
    Virology 270:251-3. 2000
    ..New information has been recently obtained on the two sides of the survival line: the edge of adaptation and the edge of extinction...
  23. ncbi request reprint [Variability and development of viral populations: assessment and implications]
    E Baranowski
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, Universidad Autonoma de Madrid, Cantoblanco, Espana
    Med Trop (Mars) 59:430-4. 1999
    ..Finally the quasi-species concept provides the basis for defining the selective factors that could influence the evolution of RNA virus and promote the emergence or reemergence of viral diseases...
  24. ncbi request reprint Biochemical and structural studies with neutralizing antibodies raised against foot-and-mouth disease virus
    E Domingo
    Centro de Biologá Molecular Servero Ochoa, Universidad Autonoma de Madrid, Cantoblanco, Spain
    Virus Res 62:169-75. 1999
    ..Essential residues at one stage of virus evolution may become dispensable at another not very distant point in the evolutionary landscape. Implications for FMDV evolution and vaccine design are discussed...
  25. ncbi request reprint Foot-and-mouth disease virus
    Esteban Domingo
    Centro de Biologia Molecular Severo Ochoa, Universidad Autonoma de Madrid, Cantoblanco, Spain
    Comp Immunol Microbiol Infect Dis 25:297-308. 2002
    ..This is relevant to the current debate on the adequacy of non-vaccination versus vaccination policies for the control of FMD...
  26. pmc Mechanisms of viral emergence
    Esteban Domingo
    Centro de Biologia Molecular Severo Ochoa, Madrid, Spain
    Vet Res 41:38. 2010
    ..This article reviews some of the mechanisms that have been identified in viral emergence, with a focus on the importance of genetic variation of viruses, and on the general concept of biological complexity...
  27. pmc Quasispecies structure and persistence of RNA viruses
    E Domingo
    Centro de Biologia Molecular Severo Ochoa, Universidad Autonoma de Madrid, Cantoblanco, Spain
    Emerg Infect Dis 4:521-7. 1998
    ....
  28. doi request reprint 5-fluorouracil in lethal mutagenesis of foot-and-mouth disease virus
    Rubén Agudo
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, Consejo Superior de Investigaciones Cientificas CSIC, Campus de Cantoblanco, 28049 Madrid, Spain
    Future Med Chem 1:529-39. 2009
    ..We suggest that structural and biochemical studies can contribute to the lead to new design of base or nucleoside analogues targeted specifically to viral polymerases...
  29. pmc Response of foot-and-mouth disease virus to increased mutagenesis: influence of viral load and fitness in loss of infectivity
    S Sierra
    Centro de Biologia Molecular Severo Ochoa, Universidad Autonoma de Madrid, Cantoblanco, 28049 Madrid, Spain
    J Virol 74:8316-23. 2000
    ..The results suggest that strategies to combine reductions of viral load and viral fitness could be effectively associated with extinction mutagenesis as a potential new antiviral strategy...
  30. pmc Antigenic heterogeneity of a foot-and-mouth disease virus serotype in the field is mediated by very limited sequence variation at several antigenic sites
    M G Mateu
    Centro de Biologia Molecular CSIC UAM, Universidad Autonoma de Madrid, Cantoblanco, Madrid, Spain
    J Virol 68:1407-17. 1994
    ..The results suggest that within a serotype of FMDV, antigenically highly divergent viruses can arise in the field by very limited sequence variation at exposed key residues of each of several antigenic sites...
  31. pmc Cell recognition by foot-and-mouth disease virus that lacks the RGD integrin-binding motif: flexibility in aphthovirus receptor usage
    E Baranowski
    Centro de Biolog ia Molecular Severo Ochoa, Universidad Aut onoma de Madrid, Cantoblanco, 28049 Madrid, Spain
    J Virol 74:1641-7. 2000
    ..These results indicate at least three different mechanisms for cell recognition by FMDV and suggest a potential for this virus to use multiple, alternative receptors for entry even into the same cell type...
  32. ncbi request reprint Antigenic properties and population stability of a foot-and-mouth disease virus with an altered Arg-Gly-Asp receptor-recognition motif
    C M Ruiz-Jarabo
    Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientificas, Universidad Autonoma de Madrid, Spain
    J Gen Virol 80:1899-909. 1999
    ..These critical replacements may yield viruses whose stability allows them to replicate efficiently and to expand the sequence repertoire of an antigenic site...
  33. ncbi request reprint Reactivity with monoclonal antibodies of viruses from an episode of foot-and-mouth disease
    M G Mateu
    Centro de Biologia Molecular CSIC UAM, Universidad Autonoma, Madrid, Spain
    Virus Res 8:261-74. 1987
    ..Thus, epidemiologically related FMDVs differ in at least one epitope critical for virus neutralization...
  34. ncbi request reprint A single amino acid substitution affects multiple overlapping epitopes in the major antigenic site of foot-and-mouth disease virus of serotype C
    M G Mateu
    Centro de Biologia Molecular, Universidad Autonoma de Madrid, Spain
    J Gen Virol 71:629-37. 1990
    ..This indicates that a minimum genetic change can result in a highly amplified phenotypic effect, as regards the antigenicity of FMDV...
  35. pmc Implications of a quasispecies genome structure: effect of frequent, naturally occurring amino acid substitutions on the antigenicity of foot-and-mouth disease virus
    M G Mateu
    Centro de Biologia Molecular, Universidad Autonoma de Madrid, Spain
    Proc Natl Acad Sci U S A 86:5883-7. 1989
    ..The results implicate the extreme heterogeneity of foot-and-mouth disease virus as an important element of viral pathogenesis...
  36. ncbi request reprint A multiply substituted G-H loop from foot-and-mouth disease virus in complex with a neutralizing antibody: a role for water molecules
    W F Ochoa
    Instituto Biología Molecular de Barcelona, Consejo Superior de Investigaciones Cientificas, Jordi Girona 18 26, 08034 Barcelona, Spain
    J Gen Virol 81:1495-505. 2000
    ..The constancy of the structure of an antigenic domain despite multiple amino acid substitutions has implications for vaccine design...
  37. pmc A single amino acid substitution in nonstructural protein 3A can mediate adaptation of foot-and-mouth disease virus to the guinea pig
    J I Núñez
    Centro de Biologia Molecular Severo Ochoa, Universidad Autonoma de Madrid, 28049 Madrid, Spain
    J Virol 75:3977-83. 2001
    ....
  38. ncbi request reprint Two mechanisms of antigenic diversification of foot-and-mouth disease virus
    M A Martinez
    Centro de Biologia Molecular CSIC UAM, Universidad Autonoma de Madrid, Spain
    Virology 184:695-706. 1991
    ..However, the screening of isolates from six decades suggests that the virus, even within the confines of a single serotype, has exploited a minimum of its potential for antigenic variation...
  39. ncbi request reprint Genetic and immunogenic variations among closely related isolates of foot-and-mouth disease virus
    M A Martinez
    Instituto Nacional de Investigaciones Agrarias, Madrid, Spain
    Gene 62:75-84. 1988
    ..Vaccines prepared with two of the FMDV C1 analyzed induce complete protection against an homologous virus but only partial protection against an heterologous virus in swine, the host from which these viruses were isolated...
  40. ncbi request reprint Molecular evolution of aphthoviruses
    E Domingo
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, Madrid, Spain
    Virus Genes 11:197-207. 1995
    ..We review recent evidence to support this proposal, and we suggest that similar concepts may apply to other RNA viruses as well...
  41. pmc Memory in viral quasispecies
    C M Ruiz-Jarabo
    Centro de Biologia Molecular Severo Ochoa, Universidad Autonoma de Madrid, Cantoblanco, 28049 Madrid, Spain
    J Virol 74:3543-7. 2000
    ..The molecular memory of the viral quasispecies influenced both the nature and the intensity of the response of the virus to a selective constraint...
  42. pmc Rapid selection of genetic and antigenic variants of foot-and-mouth disease virus during persistence in cattle
    F Gebauer
    Centro de Biologia Molecular, Universidad Autonoma de Madrid, Spain
    J Virol 62:2041-9. 1988
    ..We suggest that persistent, inapparent infections of ruminants, in addition to being a reservoir of virus, may promote the rapid selection of antigenically variant FMDVs...
  43. pmc Rapid selection in modified BHK-21 cells of a foot-and-mouth disease virus variant showing alterations in cell tropism
    C Escarmis
    Centro de Biologia Molecular Severo Ochoa, Universidad Autonoma de Madrid, 28049 Madrid, Spain
    J Virol 72:10171-9. 1998
    ..These results prove that infection of partially permissive cells can promote the rapid selection of virus variants that show alterations in cell tropism and are highly virulent for the same cells...
  44. ncbi request reprint Antibodies raised in a natural host and monoclonal antibodies recognize similar antigenic features of foot-and-mouth disease virus
    M G Mateu
    Centro de Biologia Molecular, Severo Ochoa, CSIC UAM, Universidad Autonoma de Madrid, Spain
    Virology 210:120-7. 1995
    ..In addition, the results encourage the use of extended panels of well-characterized MAbs for a precise molecular analysis of the antigenic variation of FMDV, and of other viruses, in the field...
  45. pmc Unique amino acid substitutions in the capsid proteins of foot-and-mouth disease virus from a persistent infection in cell culture
    J Diez
    Centro de Biologia Molecular, Universidad Autonoma de Madrid, Spain
    J Virol 64:5519-28. 1990
    ....
  46. pmc Evolution of the capsid protein genes of foot-and-mouth disease virus: antigenic variation without accumulation of amino acid substitutions over six decades
    M A Martinez
    Centro de Biologia Molecular, Universidad Autonoma de Madrid, Spain
    J Virol 66:3557-65. 1992
    ..Thus, the antigenic variation attained by FMDV type C over 6 decades was due to fluctuations among limited combinations of amino acid residues without net accumulation of amino acid replacements over time...
  47. ncbi request reprint Foot-and-mouth disease virus lacking the VP1 G-H loop: the mutant spectrum uncovers interactions among antigenic sites for fitness gain
    E Baranowski
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, Universidad Autonoma de Madrid, Cantoblanco, 28049 Madrid, Spain
    Virology 288:192-202. 2001
    ..The possibility of producing viable viruses lacking antigenic site A may find application in the design of new anti-FMD vaccines...
  48. pmc Unprocessed foot-and-mouth disease virus capsid precursor displays discontinuous epitopes involved in viral neutralization
    J C Saiz
    Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientificas, Universidad Autonoma de Madrid, Spain
    J Virol 68:4557-64. 1994
    ....
  49. ncbi request reprint Selection of antigenic variants of foot-and-mouth disease virus in the absence of antibodies, as revealed by an in situ assay
    J Diez
    Centro de Biologia Molecular CSIC UAM, Universidad Autonoma, Madrid, Spain
    J Gen Virol 70:3281-9. 1989
    ..We discuss models for the selection of antigenic variants of FMDV in the absence of antibodies, and implications for the antigenic diversification of RNA viruses...
  50. ncbi request reprint Direct evaluation of the immunodominance of a major antigenic site of foot-and-mouth disease virus in a natural host
    M G Mateu
    Centro de Biologia Molecular Severo Ochoa, Universidad Autonoma de Madrid, Spain
    Virology 206:298-306. 1995
    ..Thus, the major antigenic site of FMDV shows heterogeneous dominance in a natural host. Possible implications for evolution of viral quasispecies are discussed...
  51. pmc Coevolution of cells and viruses in a persistent infection of foot-and-mouth disease virus in cell culture
    J C de La Torre
    Centro de Biología Molecular Consejo Superior de Investigaciones Científicas, Universidad Autonoma de Madrid, Spain
    J Virol 62:2050-8. 1988
    ..The results suggest that coevolution of BHK-21 cells and FMDV contributes to the maintenance of persistence in cell culture...
  52. ncbi request reprint Modifications of the 5' untranslated region of foot-and-mouth disease virus after prolonged persistence in cell culture
    C Escarmis
    Centro de Biologia Molecular CSIC UAM, Universidad Autonoma, Canto Blanco, Madrid, Spain
    Virus Res 26:113-25. 1992
    ..This long poly C of R100 RNA includes a few uridine residues interspersed at fairly regular intervals. This is the longest highly homopolymeric tract described in a viral genome and, to our knowledge, in any informational biomolecule...
  53. pmc Fitness alteration of foot-and-mouth disease virus mutants: measurement of adaptability of viral quasispecies
    M A Martinez
    Departamento de Sanidad Animal, Instituto Nacional de Investigaciones Agrarias, Madrid, Spain
    J Virol 65:3954-7. 1991
    ..Thus, variants that were previously kept at low frequency in the mutant spectrum of a viral quasispecies rapidly became the master sequence of a new genomic distribution and dominated the viral population...
  54. pmc Efficient virus extinction by combinations of a mutagen and antiviral inhibitors
    N Pariente
    , , Cantoblanco, 28049 Madrid, Spain
    J Virol 75:9723-30. 2001
    ..The results suggest that combinations of mutagenic agents and antiviral inhibitors can effectively drive high-fitness virus into extinction...
  55. pmc Multiple virulence determinants of foot-and-mouth disease virus in cell culture
    E Baranowski
    Centro de Biologia Molecular Severo Ochoa, Universidad Autonoma de Madrid, 28049 Madrid, Spain
    J Virol 72:6362-72. 1998
    ....
  56. ncbi request reprint Extensive antigenic heterogeneity of foot-and-mouth disease virus of serotype C
    M G Mateu
    Centro de Biologia Molecular CSIC UAM, Universidad Autonoma de Madrid, Spain
    Virology 167:113-24. 1988
    ..It is concluded that variation of epitopes involved in neutralization of FMDV is extensive among subtypes of serotype C and also among individual isolates of one subtype...
  57. pmc A single nucleotide substitution in the internal ribosome entry site of foot-and-mouth disease virus leads to enhanced cap-independent translation in vivo
    E Martinez-Salas
    Centro de Biologia Molecular Severo Ochoa, Universidad Autonoma de Madrid, Cantoblanco, Spain
    J Virol 67:3748-55. 1993
    ..The results show that a single point mutation in an IRES element of a picornavirus can cause an increase in translation efficiency...
  58. ncbi request reprint Generation of a subtype-specific neutralization epitope in foot-and-mouth disease virus of a different subtype
    J Hernandez
    Centro de Biologia Molecular, Universidad Autonoma de Madrid, Spain
    J Gen Virol 73:213-6. 1992
    ....
  59. ncbi request reprint Antibody and host cell recognition of foot-and-mouth disease virus (serotype C) cleaved at the Arg-Gly-Asp (RGD) motif: a structural interpretation
    J Hernandez
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, Universidad Autonoma de Madrid, Cantoblanco, Spain
    J Gen Virol 77:257-64. 1996
    ....
  60. ncbi request reprint Non-additive effects of multiple amino acid substitutions on antigen-antibody recognition
    M G Mateu
    Centro de Biologia Molecular CSIC UAM, Universidad Autonoma de Madrid, Cantoblanco, Spain
    Eur J Immunol 22:1385-9. 1992
    ..The non-additive effects of multiple replacements may modulate the extent of antigenic diversification of highly variable RNA viruses, and keep viruses confined within antigenic groups by precluding linear antigenic divergence...
  61. ncbi request reprint Mutational analysis of discontinuous epitopes of foot-and-mouth disease virus using an unprocessed capsid protomer precursor
    M G Mateu
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, Cantoblanco, Madrid, Spain
    Virus Res 53:27-37. 1998
    ..The results validate site-directed mutagenesis of constructs encoding capsid precursors as an approach to probe the structure of viral discontinuous epitopes not amenable to analysis with synthetic peptides...
  62. ncbi request reprint Molecular intermediates of fitness gain of an RNA virus: characterization of a mutant spectrum by biological and molecular cloning
    A Arias
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, Universidad Autonoma de Madrid, Cantoblanco, 28049 Madrid, Spain
    J Gen Virol 82:1049-60. 2001
    ..The results underline the observation that greater insight into evolutionary processes of viruses may be gained from detailed clonal analyses of the mutant swarms at the sequence level...
  63. ncbi request reprint Long-term, large-population passage of aphthovirus can generate and amplify defective noninterfering particles deleted in the leader protease gene
    N Charpentier
    Centro de Biologia Molecular, Severo Ochoa CSIC UAM, Universidad Autonoma de Madrid, Cantoblanco, Spain
    Virology 223:10-8. 1996
    ..This is the first description of naturally occurring, defective particles of FMDV...
  64. pmc Rapid cell variation can determine the establishment of a persistent viral infection
    A M Martin Hernandez
    Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientificas UAM, Universidad Autonoma de Madrid, Spain
    Proc Natl Acad Sci U S A 91:3705-9. 1994
    ..The possible relevance of this mechanism to viral persistence in vivo is discussed...
  65. pmc Identification of an essential region for internal initiation of translation in the aphthovirus internal ribosome entry site and implications for viral evolution
    E Martinez-Salas
    Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientificas Universidad Autonoma de Madrid, Spain
    J Virol 70:992-8. 1996
    ..The results indicate a critical participation of the base of domain 3 in the activity of the aphthovirus IRES, with a strong effect of secondary or higher-order structures and minor effects of primary structure...
  66. ncbi request reprint RNA virus mutations and fitness for survival
    E Domingo
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, Universidad Autonoma de Madrid, Cantoblanco, Spain
    Annu Rev Microbiol 51:151-78. 1997
    ..Viruses occupy only a tiny portion of their potential sequence space. Such limited tolerance to mutations may open new avenues for combating viral infections...
  67. pmc Suppression of viral infectivity through lethal defection
    Ana Grande-Pérez
    Centro de Biologia Molecular Severo Ochoa, Universidad Autonoma de Madrid, Cantoblanco, 28049 Madrid, Spain
    Proc Natl Acad Sci U S A 102:4448-52. 2005
    ..The results bear on a potential application of increased mutagenesis as an antiviral strategy in that low doses of a mutagenic agent may suffice to drive persistent virus to extinction...
  68. ncbi request reprint Systematic replacement of amino acid residues within an Arg-Gly-Asp-containing loop of foot-and-mouth disease virus and effect on cell recognition
    M G Mateu
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, Universidad Autonoma de Madrid, Cantoblanco, 28049 Madrid, Spain
    J Biol Chem 271:12814-9. 1996
    ..The results support the proposal that, in spite of their involvement in antibody recognition, RGD and other FMDV loop residues are remarkably conserved because of their essential role in cell recognition...
  69. pmc Extensive cell heterogeneity during persistent infection with foot-and-mouth disease virus
    J C de La Torre
    Centro de Biologia Molecular, Universidad Autonoma, Madrid, Spain
    J Virol 63:59-63. 1989
    ..We suggest that cell heterogeneity confers a selective advantage for long-term virus and cell survival by providing the cell population with a range of responses toward FMDV...
  70. ncbi request reprint An RNA virus can adapt to the multiplicity of infection
    N Sevilla
    Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientificas, Universidad Autonoma de Madrid, Spain
    J Gen Virol 79:2971-80. 1998
    ..Therefore, different viral mutants from quasispecies replicating in vivo may be selected depending on the number of variant viruses relative to the number of susceptible cells...
  71. ncbi request reprint Large deletions in the 5'-untranslated region of foot-and-mouth disease virus of serotype C
    C Escarmis
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, Universidad Autonoma de Madrid, Spain
    Virus Res 35:155-67. 1995
    ..Implications for FMDV evolution are discussed...
  72. ncbi request reprint Genomic nucleotide sequence of a foot-and-mouth disease virus clone and its persistent derivatives. Implications for the evolution of viral quasispecies during a persistent infection
    M Toja
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, Universidad Autonoma de Madrid, 28049 Cantoblanco, Madrid, Spain
    Virus Res 64:161-71. 1999
    ....
  73. ncbi request reprint Foot-and-mouth disease virus evolution: exploring pathways towards virus extinction
    E Domingo
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, Universidad Autonoma de Madrid, Cantoblanco, 28049 Madrid, Spain
    Curr Top Microbiol Immunol 288:149-73. 2005
    ..Such a comparison is contributing to the development of new antiviral strategies based on the transition of viral replication into error catastrophe...
  74. ncbi request reprint Evolution of cell recognition by viruses
    E Baranowski
    , , Cantoblanco, 28049 Madrid, Spain
    Science 292:1102-5. 2001
    ..A capacity to exploit alternative entry pathways may reflect the ancient evolutionary origins of viruses and a possible role as agents of horizontal gene transfers among cells...
  75. pmc Evolution subverting essentiality: dispensability of the cell attachment Arg-Gly-Asp motif in multiply passaged foot-and-mouth disease virus
    M A Martinez
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, Universidad Autonoma de Madrid, 28049 Cantoblanco, Madrid, Spain
    Proc Natl Acad Sci U S A 94:6798-802. 1997
    ..Thus, variability of highly conserved motifs, even those that viruses have adapted from functional cellular motifs, can contribute to phenotypic flexibility of RNA viruses in nature...
  76. pmc Evolution of a persistent aphthovirus in cytolytic infections: partial reversion of phenotypic traits accompanied by genetic diversification
    N Sevilla
    Centro de Biologia Molecular Severo Ochoa, Universidad Autonoma de Madrid, Spain
    J Virol 70:6617-24. 1996
    ..Implications for the evolution of RNA viruses are discussed...
  77. doi request reprint An interfering activity against lymphocytic choriomeningitis virus replication associated with enhanced mutagenesis
    Veronica Martin
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, C Nicolás Cabrera, 1, Universidad Autonoma de Madrid, Cantoblanco, 28049 Madrid, Spain
    J Gen Virol 91:990-1003. 2010
    ..The results provide support for a specific interference associated with LCMV when the virus replicates in the presence of FU. The excess of interference relative to that observed in the absence of FU is necessary for LCMV extinction...
  78. ncbi request reprint Lethal mutagenesis of the prototypic arenavirus lymphocytic choriomeningitis virus (LCMV)
    Carmen M Ruiz-Jarabo
    Centro de Biología Molecular Severo Ochoa CSIC, UAM, Universidad Autonoma de Madrid, Cantoblanco, 28049 Madrid, Spain
    Virology 308:37-47. 2003
    ..Moreover, FU treatment prevented the establishment of LCMV persistent infection in mice deficient in B and T cells, suggesting the feasibility in vivo of lethal mutagenesis as a novel antiviral strategy...
  79. ncbi request reprint Multiple molecular pathways for fitness recovery of an RNA virus debilitated by operation of Muller's ratchet
    C Escarmis
    Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientificas, Cantoblanco, Universidad Autonoma de Madrid, 28049, Spain
    J Mol Biol 285:495-505. 1999
    ..The results document that an RNA virus can find multiple pathways to reach alternative high fitness peaks on the fitness landscape...
  80. ncbi request reprint Prevalence and genetic heterogeneity of the reverse transcriptase T69S-S-X insertion in pretreated HIV-infected patients
    C Briones
    Service of Infectious Diseases, Hospital Carlos III, Instituto de Salud Carlos III, Madrid, Spain
    Intervirology 44:339-43. 2001
    ..It was always found coupled to the T69S mutation. The extra amino acids were S-S in 2 subjects and S-G in the other 2. The presence of the insert was seen only in subjects previously exposed to AZT monotherapy for at least 6 months...
  81. ncbi request reprint Curing of foot-and-mouth disease virus from persistently infected cells by ribavirin involves enhanced mutagenesis
    Antero Airaksinen
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, Universidad Autonoma de Madrid, Cantoblanco, 28049 Madrid, Spain
    Virology 311:339-49. 2003
    ..The results suggest that the increased mutation frequencies were not dependent on nucleotide pool imbalances or due to selection of preexisting genomes but they were produced by a mutagenic action of ribavirin...
  82. ncbi request reprint Dynamics of mutation and recombination in a replicating population of complementing, defective viral genomes
    Juan García-Arriaza
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, Cantoblanco, E 28049 Madrid, Spain
    J Mol Biol 360:558-72. 2006
    ..The results extend the concept of a complex spectrum of mutant genomes to a complex spectrum of defective genomes in some evolutionary transitions of RNA viruses...
  83. doi request reprint Molecular characterization of a dual inhibitory and mutagenic activity of 5-fluorouridine triphosphate on viral RNA synthesis. Implications for lethal mutagenesis
    Rubén Agudo
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, Universidad Autonoma de Madrid, Cantoblanco, 28049, Madrid, Spain
    J Mol Biol 382:652-66. 2008
    ..The dual mutagenic and inhibitory activity of 5-fluorouridine triphosphate may contribute to the effective extinction of FMDV by 5-FU through virus entry into error catastrophe...
  84. ncbi request reprint Resistance to extinction of low fitness virus subjected to plaque-to-plaque transfers: diversification by mutation clustering
    Cristina Escarmis
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, Cantoblanco, Universidad Autonoma de Madrid, 28049, Spain
    J Mol Biol 315:647-61. 2002
    ..The results document resistance of viral clones to extinction, and suggest that mutation clustering may be a mechanism of genetic diversification of low fitness virus...
  85. ncbi request reprint A highly divergent antigenic site of foot-and-mouth disease virus retains its immunodominance
    B Borrego
    Centro de Biologia Molecular Severo Ochoa, Universidad Autonoma, Madrid, Spain
    Viral Immunol 8:11-8. 1995
    ..These cocktail formulations may be required to control diseases caused by FMDV and, generally, by highly variable RNA viruses, since single specificity peptides may trigger selection of vaccine-escape viral mutants...
  86. ncbi request reprint Persistence of foot-and-mouth disease virus in cell culture revisited: implications for contingency in evolution
    Monica Herrera
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, Universidad Autonoma de Madrid, Cantoblanco, 28049 Madrid, Spain
    J Gen Virol 89:232-44. 2008
    ....
  87. doi request reprint No evidence of selection for mutational robustness during lethal mutagenesis of lymphocytic choriomeningitis virus
    Veronica Martin
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, C Nicolás Cabrera, Universidad Autonoma de Madrid, Cantoblanco, 28049, Madrid, Spain
    Virology 378:185-92. 2008
    ..We consider mechanisms by which viruses could escape extinction by lethal mutagenesis, and provide evidence that mutational robustness is unlikely to be one of them...
  88. pmc Mutagenesis-induced, large fitness variations with an invariant arenavirus consensus genomic nucleotide sequence
    Ana Grande-Pérez
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, Madrid, Spain
    J Virol 79:10451-9. 2005
    ..The results suggest that low-level mutagenesis to enrich the viral population with defector, interfering genomes harboring limited numbers of mutations may mediate the loss of infectivity that accompanies viral extinction...
  89. ncbi request reprint Repeated bottleneck transfers can lead to non-cytocidal forms of a cytopathic virus: implications for viral extinction
    Cristina Escarmis
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, Campus de Cantoblanco, 28049 Madrid, Spain
    J Mol Biol 376:367-79. 2008
    ..The results document a major phenotypic transition of a virus as a result of serial bottleneck events...
  90. doi request reprint Influence of the mutant spectrum in viral evolution: focused selection of antigenic variants in a reconstructed viral quasispecies
    Veronica Martin
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, C Nicolás Cabrera, Universidad Autonoma de Madrid, Cantoblanco, Madrid, Spain
    Mol Biol Evol 25:1544-54. 2008
    ....
  91. ncbi request reprint RNA virus evolution, population dynamics, and nutritional status
    E Domingo
    Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientificas, Universidad Autonoma de Madrid, Spain
    Biol Trace Elem Res 56:23-30. 1997
    ..Recent results suggest links between nutritional deficiencies and the generation of variant viruses, a possibility that is addressed in the present article...
  92. ncbi request reprint Viral fitness can influence the repertoire of virus variants selected by antibodies
    Veronica Martin
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, Cantoblanco, E 28049 Madrid, Spain
    J Mol Biol 362:44-54. 2006
    ..We discuss the significance of these results in relation to antibody selection, and to other selective forces likely encountered by viral quasispecies in vivo...
  93. pmc Foot-and-mouth disease virus mutant with decreased sensitivity to ribavirin: implications for error catastrophe
    Macarena Sierra
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, Cantoblanco, E 28049 Madrid, Spain
    J Virol 81:2012-24. 2007
    ..Implications for lethal mutagenesis are discussed...
  94. pmc Guinea pig-adapted foot-and-mouth disease virus with altered receptor recognition can productively infect a natural host
    Jose I Núñez
    Centro de Biologia Molecular Severo Ochoa, Cantoblanco, 28049 Madrid, Spain
    J Virol 81:8497-506. 2007
    ..These observations illustrate how the appearance of minority variant viruses in an unnatural host can result in the dominance of these viruses on reinfection of the original host species...
  95. pmc Deletion mutants of VPg reveal new cytopathology determinants in a picornavirus
    Armando Arias
    Departamento de Virología y Microbiología, Centro de Biologia Molecular Severo Ochoa CSIC UAM, Consejo Superior de Investigaciones Cientificas CSIC, Campus de Cantoblanco, Madrid, Spain
    PLoS ONE 5:e10735. 2010
    ..Foot-and-mouth disease virus (FMDV) is exceptional among picornaviruses in that its genome encodes 3 copies of VPg. Why FMDV encodes three VPgs is unknown...
  96. pmc Competition-colonization dynamics in an RNA virus
    Samuel Ojosnegros
    Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientificas Universidad Autonoma de Madrid, Campus de Cantoblanco, 28049 Madrid, Spain
    Proc Natl Acad Sci U S A 107:2108-12. 2010
    ..Our results suggest a model for the evolution of virulence in viruses based on internal interactions within mutant spectra of viral quasispecies...
  97. pmc Molecular basis for a lack of correlation between viral fitness and cell killing capacity
    Monica Herrera
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, Cantoblanco, Madrid, Spain
    PLoS Pathog 3:e53. 2007
    ..This lack of correlation is relevant to current models of attenuation and virulence in that virus de-adaptation need not entail a decrease of virulence...
  98. ncbi request reprint Invariant aphthovirus consensus nucleotide sequence in the transition to error catastrophe
    Claudia González-López
    Centro de Biologia Molecular Severo Ochoa CSIC UAM, Universidad Autonoma de Madrid, Cantoblanco, Spain
    Infect Genet Evol 5:366-74. 2005
    ..Thus, increase in mutant spectrum complexity and invariance of the consensus sequence characterizes FMDV extinction through error catastrophe...
  99. ncbi request reprint Expansion of host-cell tropism of foot-and-mouth disease virus despite replication in a constant environment
    Carmen M Ruiz-Jarabo
    Centro de Biologia Molecular Severo Ochoa, Universidad Autonoma de Madrid, Cantoblanco, 28049 Madrid, Spain
    J Gen Virol 85:2289-97. 2004
    ....
  100. pmc Mutagenesis versus inhibition in the efficiency of extinction of foot-and-mouth disease virus
    Nonia Pariente
    Centro de Biologia Molecular Severo Ochoa, Universidad Autonoma de Madrid, Cantoblanco, 28049 Madrid, Spain
    J Virol 77:7131-8. 2003
    ....
  101. pmc Modeling viral genome fitness evolution associated with serial bottleneck events: evidence of stationary states of fitness
    Ester Lázaro
    Centro de Astrobiologia CSIC INTA, 28850 Torrejon de Ardoz, Madrid, Spain
    J Virol 76:8675-81. 2002
    ..In particular, the size of the bottleneck is critical for determining the trend of fitness evolution...