José Antonio Daròs
Cordero T, Aragonés V, Daròs J. Large-scale Production of Recombinant RNAs on a Circular Scaffold Using a Viroid-derived System in Escherichia coli. J Vis Exp. 2018;: pubmed publisher
..The recombinant RNA can be extracted from the bacterial cells and separated from the bulk of bacterial RNAs taking advantage of its circularity. ..
Majer E, Navarro J, DarÃ²s J. A potyvirus vector efficiently targets recombinant proteins to chloroplasts, mitochondria and nuclei in plant cells when expressed at the amino terminus of the polyprotein. Biotechnol J. 2015;10:1792-802 pubmed publisher
..These observations will help in the design of potyvirus-based vectors able to coexpress several proteins while targeting different subcellular localizations, as required in plant metabolic engineering. ..
Carbonell A, López C, Daròs J. Fast-Forward Identification of Highly Effective Artificial Small RNAs Against Different Tomato spotted wilt virus Isolates. Mol Plant Microbe Interact. 2019;32:142-156 pubmed publisher
..These results highlight the usefulness of this high-throughput methodology for the fast-forward identification of artificial sRNAs with high antiviral activity prior to time-consuming generation of stably transformed plants. ..
DarÃ²s J. Eggplant latent viroid: a friendly experimental system in the family Avsunviroidae. Mol Plant Pathol. 2016;17:1170-7 pubmed publisher
..A very narrow host range seems to be a common property in members of the family Avsunviroidae. ELVd infections of eggplants are apparently symptomless. ELVd is transmitted mechanically and by seed. http://subviral.med.uottawa.ca. ..
MartÃnez F, Rodrigo G, AragonÃ©s V, Ruiz M, Lodewijk I, FernÃ¡ndez U, et al
. Interaction network of tobacco etch potyvirus NIa protein with the host proteome during infection. BMC Genomics. 2016;17:87 pubmed publisher
..Many of these proteins are connected with the phytohormone ethylene. Potyviral NIa targets many host elements during infection, establishing a network in which information is efficiently transmitted. ..
Daròs J, Aragonés V, Cordero T. A viroid-derived system to produce large amounts of recombinant RNA in Escherichia coli. Sci Rep. 2018;8:1904 pubmed publisher
..The mature viroid forms and the protein likely form a ribonucleoprotein complex that transitorily accumulates in E. coli cells at extraordinarily amounts. ..
Gas M, Molina Serrano D, Hernandez C, Flores R, Daròs J. Monomeric linear RNA of citrus exocortis viroid resulting from processing in vivo has 5'-phosphomonoester and 3'-hydroxyl termini: implications for the RNase and RNA ligase involved in replication. J Virol. 2008;82:10321-5 pubmed publisher
Martinez F, Daròs J. Tobacco etch virus protein P1 traffics to the nucleolus and associates with the host 60S ribosomal subunits during infection. J Virol. 2014;88:10725-37 pubmed publisher
..Our experiments also showed that P1 protein binds host ribosomes during infection. Based on these findings and other in vitro experiments we propose that P1 protein stimulates translation of viral proteins during infection. ..
Rodrigo G, Daròs J, Elena S. Virus-host interactome: Putting the accent on how it changes. J Proteomics. 2017;156:1-4 pubmed publisher