Thomas Graf

Summary

Affiliation: Center for Genomic Regulation
Country: Spain

Publications

  1. doi request reprint B young again
    Thomas Graf
    Center for Genomic Regulation, Carrer Dr Aiguader 88, E 08003 Barcelona, Spain
    Immunity 28:606-8. 2008
  2. doi request reprint Forcing cells to change lineages
    Thomas Graf
    Center for Genomic Regulation and ICREA, 08003 Barcelona, Spain
    Nature 462:587-94. 2009
  3. pmc Induced pluripotent stem cell-derived human platelets: one step closer to the clinic
    Christos Gekas
    Differentiation and Cancer Program, Center for Genomic Regulation, 08003 Barcelona, Spain
    J Exp Med 207:2781-4. 2010
  4. pmc Fibroblast-derived induced pluripotent stem cells show no common retroviral vector insertions
    Florencio Varas
    Differentiation and Cancer, Center for Genomic Regulation and Pompeu Fabra University, Barcelona, Spain
    Stem Cells 27:300-6. 2009
  5. doi request reprint Heterogeneity of embryonic and adult stem cells
    Thomas Graf
    Center for Genomic Regulation, ICREA and Pompeu Fabra University, 08003 Barcelona, Spain
    Cell Stem Cell 3:480-3. 2008
  6. doi request reprint Historical origins of transdifferentiation and reprogramming
    Thomas Graf
    ICREA Professor, Center for Genomic Regulation and Pompeu Fabra University, 08003 Barcelona, Spain
    Cell Stem Cell 9:504-16. 2011
  7. doi request reprint C/EBPa-mediated activation of microRNAs 34a and 223 inhibits Lef1 expression to achieve efficient reprogramming into macrophages
    Javier Rodriguez-Ubreva
    Chromatin and Disease Group, Cancer Epigenetics and Biology Program PEBC, Bellvitge Biomedical Research Institute IDIBELL, L Hospitalet de Llobregat, Barcelona, Spain
    Mol Cell Biol 34:1145-57. 2014
  8. pmc Tet2 facilitates the derepression of myeloid target genes during CEBPα-induced transdifferentiation of pre-B cells
    Eric M Kallin
    Gene Regulation, Stem Cells and Cancer Program, Center for Genomic Regulation, and Pompeu Fabra University, Barcelona, Spain
    Mol Cell 48:266-76. 2012
  9. pmc HDAC7 is a repressor of myeloid genes whose downregulation is required for transdifferentiation of pre-B cells into macrophages
    Bruna Barneda-Zahonero
    Cancer Epigenetics and Biology Program PEBC, Bellvitge Biomedical Research Institute IDIBELL, Barcelona, Spain
    PLoS Genet 9:e1003503. 2013
  10. doi request reprint CD41 expression marks myeloid-biased adult hematopoietic stem cells and increases with age
    Christos Gekas
    Gene Regulation, Stem Cells and Cancer Program, Centre for Genomic Regulation, Barcelona, Spain
    Blood 121:4463-72. 2013

Collaborators

Detail Information

Publications37

  1. doi request reprint B young again
    Thomas Graf
    Center for Genomic Regulation, Carrer Dr Aiguader 88, E 08003 Barcelona, Spain
    Immunity 28:606-8. 2008
    ..Hanna et al. (2008) report in a recent issue of Cell that a defined set of transcription factors can reprogram mature B cells back to pluripotent stem cells...
  2. doi request reprint Forcing cells to change lineages
    Thomas Graf
    Center for Genomic Regulation and ICREA, 08003 Barcelona, Spain
    Nature 462:587-94. 2009
    ..The lessons learnt provide a rubric for how cells may be manipulated for therapeutic purposes...
  3. pmc Induced pluripotent stem cell-derived human platelets: one step closer to the clinic
    Christos Gekas
    Differentiation and Cancer Program, Center for Genomic Regulation, 08003 Barcelona, Spain
    J Exp Med 207:2781-4. 2010
    ..Notably, their lack of nucleus renders platelets unable to retain the pluripotent or tumorigenic properties of iPS cells...
  4. pmc Fibroblast-derived induced pluripotent stem cells show no common retroviral vector insertions
    Florencio Varas
    Differentiation and Cancer, Center for Genomic Regulation and Pompeu Fabra University, Barcelona, Spain
    Stem Cells 27:300-6. 2009
    ..We conclude that Oct4, Sox2, Klf4, and c-Myc are sufficient to promote fibroblast-to-iPS cell reprogramming and propose that the observed low reprogramming frequencies may have alternative explanations...
  5. doi request reprint Heterogeneity of embryonic and adult stem cells
    Thomas Graf
    Center for Genomic Regulation, ICREA and Pompeu Fabra University, 08003 Barcelona, Spain
    Cell Stem Cell 3:480-3. 2008
    ..These cell states show a bias in their differentiation potential and correlate with specific patterns of transcription factor expression and chromatin modifications...
  6. doi request reprint Historical origins of transdifferentiation and reprogramming
    Thomas Graf
    ICREA Professor, Center for Genomic Regulation and Pompeu Fabra University, 08003 Barcelona, Spain
    Cell Stem Cell 9:504-16. 2011
    ..In this article, I give a historical and personal perspective of the events that set the stage for our current understanding of cellular reprogramming...
  7. doi request reprint C/EBPa-mediated activation of microRNAs 34a and 223 inhibits Lef1 expression to achieve efficient reprogramming into macrophages
    Javier Rodriguez-Ubreva
    Chromatin and Disease Group, Cancer Epigenetics and Biology Program PEBC, Bellvitge Biomedical Research Institute IDIBELL, L Hospitalet de Llobregat, Barcelona, Spain
    Mol Cell Biol 34:1145-57. 2014
    ....
  8. pmc Tet2 facilitates the derepression of myeloid target genes during CEBPα-induced transdifferentiation of pre-B cells
    Eric M Kallin
    Gene Regulation, Stem Cells and Cancer Program, Center for Genomic Regulation, and Pompeu Fabra University, Barcelona, Spain
    Mol Cell 48:266-76. 2012
    ..Activation of these target genes was accompanied by rapid increases of promoter hydroxy-methylation. Our observations indicate that Tet2 helps CEBPα rapidly derepress myeloid genes during the conversion of pre-B cells into macrophages...
  9. pmc HDAC7 is a repressor of myeloid genes whose downregulation is required for transdifferentiation of pre-B cells into macrophages
    Bruna Barneda-Zahonero
    Cancer Epigenetics and Biology Program PEBC, Bellvitge Biomedical Research Institute IDIBELL, Barcelona, Spain
    PLoS Genet 9:e1003503. 2013
    ..Thus, in B cells HDAC7 is a transcriptional repressor of undesirable genes. Our findings uncover a novel role for HDAC7 in maintaining the identity of a particular cell type by silencing lineage-inappropriate genes...
  10. doi request reprint CD41 expression marks myeloid-biased adult hematopoietic stem cells and increases with age
    Christos Gekas
    Gene Regulation, Stem Cells and Cancer Program, Centre for Genomic Regulation, Barcelona, Spain
    Blood 121:4463-72. 2013
    ..In summary, our data provide a novel marker to identify a myeloid-biased HSC subtype that becomes prevalent with age and highlights the dogma of HSC regulation by their progeny...
  11. pmc CCAAT/enhancer binding protein alpha (C/EBP(alpha))-induced transdifferentiation of pre-B cells into macrophages involves no overt retrodifferentiation
    Alessandro di Tullio
    Cancer and Differentiation Program, Center for Genomic Regulation and Pompeu Fabra University, 08003 Barcelona, Spain
    Proc Natl Acad Sci U S A 108:17016-21. 2011
    ..Together, our findings are consistent with the notion that the conversion from pre-B cells to macrophages is mostly direct and does not involve overt retrodifferentiation...
  12. doi request reprint C/EBPα poises B cells for rapid reprogramming into induced pluripotent stem cells
    Bruno Di Stefano
    1 Gene Regulation, Stem Cells and Cancer Programme, Centre for Genomic Regulation CRG, Dr Aiguader 88, 08003 Barcelona, Spain 2 Universitat Pompeu Fabra UPF, Dr Aiguader 88, 08003 Barcelona, Spain
    Nature 506:235-9. 2014
    ..The rapid iPS reprogramming approach described here should help to fully elucidate the process and has potential clinical applications. ..
  13. doi request reprint C/EBPα induces highly efficient macrophage transdifferentiation of B lymphoma and leukemia cell lines and impairs their tumorigenicity
    Francesca Rapino
    Center for Genomic Regulation, Universidad Pompeu Fabra and Institució Catalana de Recerca i Estudis Avançats, Dr Aiguader 88, 08003 Barcelona, Spain
    Cell Rep 3:1153-63. 2013
    ....
  14. doi request reprint Musashi 2 is a regulator of the HSC compartment identified by a retroviral insertion screen and knockout mice
    Luisa de Andres-Aguayo
    Differentiation and Cancer Program, Center for Genomic Regulation and UPF, Barcelona, Spain
    Blood 118:554-64. 2011
    ..Our data indicate that Msi2 maintains the stem cell compartment mainly by regulating the proliferation of primitive progenitors downstream of LT-HSCs...
  15. doi request reprint Zrf1 is required to establish and maintain neural progenitor identity
    Luigi Aloia
    Centre for Genomic Regulation CRG, Universitat Pompeu Fabra, 08003 Barcelona, Spain
    Genes Dev 28:182-97. 2014
    ..Depletion of Zrf1 in vivo impairs the expression of key self-renewal regulators and Wnt ligand genes in RGCs. Thus, we demonstrate that Zrf1 plays an essential role in NPC generation and maintenance. ..
  16. pmc Pre-B cell to macrophage transdifferentiation without significant promoter DNA methylation changes
    Javier Rodriguez-Ubreva
    Chromatin and Disease Group, Cancer Epigenetics and Biology Programme PEBC, Bellvitge Biomedical Research Institute IDIBELL, 08907 L Hospitalet de Llobregat, Barcelona, Spain
    Nucleic Acids Res 40:1954-68. 2012
    ....
  17. doi request reprint C/EBPα bypasses cell cycle-dependency during immune cell transdifferentiation
    Alessandro di Tullio
    Gene Regulation, Stem Cells and Cancer Program, Center for Genomic Regulation and Pompeu Fabra University, Barcelona, Spain
    Cell Cycle 11:2739-46. 2012
    ....
  18. ncbi request reprint Stepwise reprogramming of B cells into macrophages
    Huafeng Xie
    Department of Developmental and Molecular Biology, Albert Einstein College of Medicine Cancer Research Center, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Cell 117:663-76. 2004
    ..Our observations indicate that C/EBPalpha and beta remodel the transcription network of B cells into that of macrophages through a series of parallel and sequential changes that require endogenous PU.1...
  19. ncbi request reprint Determinants of lymphoid-myeloid lineage diversification
    Catherine V Laiosa
    Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Annu Rev Immunol 24:705-38. 2006
    ....
  20. pmc PU.1 and C/EBPalpha/beta convert fibroblasts into macrophage-like cells
    Ru Feng
    Albert Einstein College of Medicine, 1300 Morris Park Avenue, New York, NY 10461, USA
    Proc Natl Acad Sci U S A 105:6057-62. 2008
    ..Our data suggest that it might become possible to induce the transdifferentiation of skin-derived fibroblasts into cell types desirable for tissue regeneration...
  21. doi request reprint Immunology: blood lines redrawn
    Thomas Graf
    Nature 452:702-3. 2008
  22. pmc Making eosinophils through subtle shifts in transcription factor expression
    Kelly McNagny
    The Biomedical Research Centre, University of British Columbia, Vancouver, BC V6T 1Z3, Canada
    J Exp Med 195:F43-7. 2002
  23. ncbi request reprint Myeloid or lymphoid promiscuity as a critical step in hematopoietic lineage commitment
    Toshihiro Miyamoto
    Departments of Pathology and Developmental Biology, Stanford University School of Medicine, Palo Alto, CA 94305, USA
    Dev Cell 3:137-47. 2002
    ..Thus, the accessibility for multiple myeloid or lymphoid programs promiscuously may allow flexibility in fate commitments at these multipotent stages...
  24. ncbi request reprint Distinguishable live erythroid and myeloid cells in beta-globin ECFP x lysozyme EGFP mice
    Susanne Heck
    Department of Molecular and Developmental Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Blood 101:903-6. 2003
    ..The new mouse lines should become useful tools to dissect the branching between erythroid and myelomonocytic cells during in vitro differentiation of definitive multipotent progenitors...
  25. ncbi request reprint E26 leukemia virus converts primitive erythroid cells into cycling multilineage progenitors
    Kelly M McNagny
    Biomedical Research Centre, Department of Medical Genetics, University of British Columbia, Vancouver, Canada
    Blood 101:1103-10. 2003
    ..These results suggest that the Myb-Ets oncoprotein of the E26 leukemia virus converts primitive erythroid cells into proliferating definitive-type multipotent hematopoietic progenitors...
  26. ncbi request reprint Increased inflammation in lysozyme M-deficient mice in response to Micrococcus luteus and its peptidoglycan
    Tomas Ganz
    Department of Medicine, David Geffen School of Medicine, Los Angeles, CA 90095 1690, USA
    Blood 101:2388-92. 2003
    ..Our data indicate that tissue injury is normally limited by prompt degradation of bacterial macromolecules that trigger innate immunity and inflammation...
  27. ncbi request reprint MafB deficiency causes defective respiratory rhythmogenesis and fatal central apnea at birth
    Bruno Blanchi
    Centre d immunologie de Marseille Luminy, CNRS INSERM Université Mediterrané, Campus de Luminy, Case 906, 13288 Marseille Cedex 09, France
    Nat Neurosci 6:1091-100. 2003
    ..Our results show an essential role of MafB in central respiratory control, possibly involving the specification of rhythmogenic preBötC neurons...
  28. ncbi request reprint A paracrine loop between tumor cells and macrophages is required for tumor cell migration in mammary tumors
    Jeffrey Wyckoff
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Cancer Res 64:7022-9. 2004
    ..This work provides the first direct evidence for a synergistic interaction between macrophages and tumor cells during cell migration in vivo and indicates a mechanism for how macrophages may contribute to metastasis...
  29. ncbi request reprint Fluorescent protein-cell labeling and its application in time-lapse analysis of hematopoietic differentiation
    Matthias Stadtfeld
    Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, Bronx, NY, USA
    Methods Mol Med 105:395-412. 2005
    ..Finally, we make recommendations about which of these filters to choose when working with specific fluorescent proteins...
  30. pmc PU.1 is not strictly required for B cell development and its absence induces a B-2 to B-1 cell switch
    Min Ye
    Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Exp Med 202:1411-22. 2005
    ..Furthermore, we found that B-2 cells express substantially more PU.1 than B-1 cells, which is consistent with the idea that maintenance of the B-2 cell phenotype requires relatively high levels of PU.1, but B-1 cells require little...
  31. pmc Characterization of the megakaryocyte demarcation membrane system and its role in thrombopoiesis
    Harald Schulze
    Dana Farber Cancer Institute, One Jimmy Fund Way, Boston, MA 02115, USA
    Blood 107:3868-75. 2006
    ..These observations collectively suggest a signaling pathway wherein PI-4,5-P(2) might facilitate DMS development and local assembly of actin fibers in preparation for platelet biogenesis...
  32. ncbi request reprint Reprogramming of committed T cell progenitors to macrophages and dendritic cells by C/EBP alpha and PU.1 transcription factors
    Catherine V Laiosa
    Department of Developmental and Molecular Biology and the Albert Einstein Cancer Center, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Immunity 25:731-44. 2006
    ..1 in multilineage precursors...
  33. ncbi request reprint Klf2 is an essential regulator of vascular hemodynamic forces in vivo
    John S Lee
    Department of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
    Dev Cell 11:845-57. 2006
    ..These findings identify Klf2 as an essential hemodynamic regulator in vivo and suggest that hemodynamic regulation in response to fluid shear stress is required for cardiovascular development and function...
  34. ncbi request reprint Early decisions in lymphoid development
    Min Ye
    Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, New York Bronx, NY 10461, USA
    Curr Opin Immunol 19:123-8. 2007
    ..In conclusion, although a lot is known about B and T cell commitment, more work needs to be done to clarify the earliest steps in lymphoid specification...
  35. ncbi request reprint CD41-YFP mice allow in vivo labeling of megakaryocytic cells and reveal a subset of platelets hyperreactive to thrombin stimulation
    Jinghang Zhang
    Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, Bronx, NY, USA
    Exp Hematol 35:490-499. 2007
    ..Development of a mouse line permitting live imaging of cells expressing CD41/GpIIb as a means to study megakaryopoiesis...
  36. doi request reprint Reciprocal activation of GATA-1 and PU.1 marks initial specification of hematopoietic stem cells into myeloerythroid and myelolymphoid lineages
    Yojiro Arinobu
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    Cell Stem Cell 1:416-27. 2007
    ..1 primarily organizes the hematopoietic lineage fate decision to form the earliest hematopoietic branchpoint that comprises isolatable myeloerythroid and myelolymphoid progenitor populations...
  37. ncbi request reprint Differentiation plasticity of hematopoietic cells
    Thomas Graf
    Albert Einstein College of Medicine, Bronx, NY, USA
    Blood 99:3089-101. 2002