Rafael Canton

Summary

Country: Spain

Publications

  1. ncbi Resistance trends in Moraxella catarrhalis (PROTEKT years 1-3 [1999-2002])
    R Canton
    Servicio de Microbiologia, Hospital Universitario Ramon y Cajal, Carretera de Colmenar Km 9 100, 28034 Madrid, Spain
    J Chemother 16:63-70. 2004
  2. pmc Integron content of extended-spectrum-beta-lactamase-producing Escherichia coli strains over 12 years in a single hospital in Madrid, Spain
    Elisabete Machado
    Hospital Universitario Ramon y Cajal, IMSALUD, Madrid 28034, Spain
    Antimicrob Agents Chemother 49:1823-9. 2005
  3. pmc Clinical and microbiological features of a cystic fibrosis patient chronically colonized with Pandoraea sputorum identified by combining 16S rRNA sequencing and matrix-assisted laser desorption ionization-time of flight mass spectrometry
    A Fernández-Olmos
    Servicio de Microbiología and CIBER en Epidemiología y Salud Pública CIBERESP, Instituto Ramón y Cajal de Investigación Sanitaria IRYCIS and Hospital Universitario Ramón y Cajal, Madrid, Spain
    J Clin Microbiol 50:1096-8. 2012
  4. pmc Fecal carriage of carbapenemase-producing Enterobacteriaceae: a hidden reservoir in hospitalized and nonhospitalized patients
    Desirèe Gijón
    Servicio de Microbiología and CIBER en Epidemiología y Salud Pública and Instituto Ramón y Cajal de Investigación Sanitaria, Madrid, Spain
    J Clin Microbiol 50:1558-63. 2012
  5. doi Global antimicrobial resistance: from surveillance to stewardship. Part 2: stewardship initiatives
    Rafael Canton
    Instituto Ramón y Cajal de Investigación Sanitaria, Hospital Universitario Ramon y Cajal, Carretera de Colmenar Km 9 1, 28034 Madrid, Spain
    Expert Rev Anti Infect Ther 10:1375-7. 2012
  6. doi Global antimicrobial resistance: from surveillance to stewardship. Part 1: surveillance and risk factors for resistance
    Rafael Canton
    Hospital Universitario Ramon y Cajal, Madrid, Spain
    Expert Rev Anti Infect Ther 10:1269-71. 2012
  7. pmc Th1 and Th17 hypercytokinemia as early host response signature in severe pandemic influenza
    Jesus F Bermejo-Martin
    National Centre of Influenza, Hospital Clinico Universitario de Valladolid, Valladolid, Spain
    Crit Care 13:R201. 2009
  8. pmc A potential role for daptomycin in enterococcal infections: what is the evidence?
    Rafael Canton
    Servicio de Microbiologia, Hospital Universitario Ramón y Cajal and Instituto Ramón y Cajal de Investigación Sanitaria IRYCIS, Madrid, Spain
    J Antimicrob Chemother 65:1126-36. 2010
  9. ncbi [Proteus penneri]
    Rafael Canton
    Servicio de Microbiologia, Hospital Universitario Ramon y Cajal, Madrid, Espana
    Enferm Infecc Microbiol Clin 24:8-13. 2006
  10. doi [Interpretive reading of the antibiogram: a clinical necessity]
    Rafael Canton
    Servicio de Microbiologia, Hospital Ramon y Cajal, Madrid, Espana
    Enferm Infecc Microbiol Clin 28:375-85. 2010

Detail Information

Publications95

  1. ncbi Resistance trends in Moraxella catarrhalis (PROTEKT years 1-3 [1999-2002])
    R Canton
    Servicio de Microbiologia, Hospital Universitario Ramon y Cajal, Carretera de Colmenar Km 9 100, 28034 Madrid, Spain
    J Chemother 16:63-70. 2004
  2. pmc Integron content of extended-spectrum-beta-lactamase-producing Escherichia coli strains over 12 years in a single hospital in Madrid, Spain
    Elisabete Machado
    Hospital Universitario Ramon y Cajal, IMSALUD, Madrid 28034, Spain
    Antimicrob Agents Chemother 49:1823-9. 2005
    ....
  3. pmc Clinical and microbiological features of a cystic fibrosis patient chronically colonized with Pandoraea sputorum identified by combining 16S rRNA sequencing and matrix-assisted laser desorption ionization-time of flight mass spectrometry
    A Fernández-Olmos
    Servicio de Microbiología and CIBER en Epidemiología y Salud Pública CIBERESP, Instituto Ramón y Cajal de Investigación Sanitaria IRYCIS and Hospital Universitario Ramón y Cajal, Madrid, Spain
    J Clin Microbiol 50:1096-8. 2012
    ..Decreased lung function improved after 1 year of azithromycin and inhaled 7%-hypertonic saline treatment...
  4. pmc Fecal carriage of carbapenemase-producing Enterobacteriaceae: a hidden reservoir in hospitalized and nonhospitalized patients
    Desirèe Gijón
    Servicio de Microbiología and CIBER en Epidemiología y Salud Pública and Instituto Ramón y Cajal de Investigación Sanitaria, Madrid, Spain
    J Clin Microbiol 50:1558-63. 2012
    ..Dissemination of bla(VIM-1) class-1 integrons within highly transferable plasmids in a polyclonal population has potentially contributed to the maintenance and spread of CPE...
  5. doi Global antimicrobial resistance: from surveillance to stewardship. Part 2: stewardship initiatives
    Rafael Canton
    Instituto Ramón y Cajal de Investigación Sanitaria, Hospital Universitario Ramon y Cajal, Carretera de Colmenar Km 9 1, 28034 Madrid, Spain
    Expert Rev Anti Infect Ther 10:1375-7. 2012
    ..The authors also report new data on antibiotic prescribing in primary care and latest research in the development of novel antibiotics for the future...
  6. doi Global antimicrobial resistance: from surveillance to stewardship. Part 1: surveillance and risk factors for resistance
    Rafael Canton
    Hospital Universitario Ramon y Cajal, Madrid, Spain
    Expert Rev Anti Infect Ther 10:1269-71. 2012
    ..The authors also focus on the spread of nonmetallo-β-lactamase carbapenemases, and likely risk factors for resistance...
  7. pmc Th1 and Th17 hypercytokinemia as early host response signature in severe pandemic influenza
    Jesus F Bermejo-Martin
    National Centre of Influenza, Hospital Clinico Universitario de Valladolid, Valladolid, Spain
    Crit Care 13:R201. 2009
    ..We utilize here systemic cytokine and antibody levels in evaluating differences in early immune response in both mild and severe patients infected with nvH1N1...
  8. pmc A potential role for daptomycin in enterococcal infections: what is the evidence?
    Rafael Canton
    Servicio de Microbiologia, Hospital Universitario Ramón y Cajal and Instituto Ramón y Cajal de Investigación Sanitaria IRYCIS, Madrid, Spain
    J Antimicrob Chemother 65:1126-36. 2010
    ..This growing body of evidence provides useful insights regarding the efficacy of daptomycin against enterococci in clinical settings...
  9. ncbi [Proteus penneri]
    Rafael Canton
    Servicio de Microbiologia, Hospital Universitario Ramon y Cajal, Madrid, Espana
    Enferm Infecc Microbiol Clin 24:8-13. 2006
    ..This phenotype resembles the production of an extended spectrum beta-lactamase. Like other Proteus species, P. penneri is resistant to tetracyclines and should be considered resistant to nitrofurantoin...
  10. doi [Interpretive reading of the antibiogram: a clinical necessity]
    Rafael Canton
    Servicio de Microbiologia, Hospital Ramon y Cajal, Madrid, Espana
    Enferm Infecc Microbiol Clin 28:375-85. 2010
    ..The interpretive reading of the antibiogram transcends the microbiologist's clinical role and is essential for clinical decisions...
  11. ncbi Role of the microbiology laboratory in infectious disease surveillance, alert and response
    R Canton
    Hospital Universitario Ramon y Cajal, Madrid, Spain
    Clin Microbiol Infect 11:3-8. 2005
    ....
  12. doi [Current panorama of the teaching of microbiology and parasitology in Spain]
    Rafael Canton
    CIBER en Investigación y Salud Pública, Instituto Ramón y Cajal de Investigación Sanitaria, Hospital Universitario Ramon y Cajal, Madrid, Espana
    Enferm Infecc Microbiol Clin 28:16-24. 2010
    ..Essential elements in the training of specialists in clinical microbiology are a close relationship between the laboratory and the clinical center and collaboration with other specialists...
  13. doi [Chronic bronchial infection: the problem of Pseudomonas aeruginosa]
    Rafael Canton
    Servicio de Microbiología y CIBER en Epidemiología y Salud Pública, Instituto Ramón y Cajal de Investigación Sanitaria, Hospital Universitario Ramon y Cajal, Madrid, Espana
    Arch Bronconeumol 47:8-13. 2011
    ..Antimicrobial treatment in patients with bronchiectasis and P. aeruginosa colonization should be based on antimicrobial agents, alone or in combination, that do not lose activity when acting on biofilms...
  14. doi Emerging resistance in Gram-negative pathogens and implications for clinical practice
    Rafael Canton
    Universitario Ramón y Cajal, Madrid, Spain
    Future Microbiol 6:19-22. 2011
    ....
  15. ncbi Antimicrobial therapy for pulmonary pathogenic colonisation and infection by Pseudomonas aeruginosa in cystic fibrosis patients
    R Canton
    Servicio de Microbiologia, Hospital Universitario Ramon y Cajal, Madrid, Spain
    Clin Microbiol Infect 11:690-703. 2005
    ..If multiresistant P. aeruginosa is isolated, antimicrobial agents that retain activity are recommended and epidemiological control measures should be established...
  16. doi Emergence and spread of antibiotic resistance following exposure to antibiotics
    Rafael Canton
    Servicio de Microbiología and CIBER en Epidemiología y Salud Pública, Hospital Universitario Ramón y Cajal and Instituto Ramón y Cajal de Investigación Sanitaria, Madrid, Spain
    FEMS Microbiol Rev 35:977-91. 2011
    ..Emergence of a resistant bacterial subpopulation within a susceptible wild-type population can be restricted with a regimen using an antibiotic dose that is sufficiently high to inhibit both susceptible and resistant bacteria...
  17. doi Co-resistance: an opportunity for the bacteria and resistance genes
    Rafael Canton
    Servicio de Microbiología and CIBER en Epidemiología y Salud Pública, Hospital Universitario Ramón y Cajal and Instituto Ramón y Cajal de Investigación Sanitaria, Carretera Colmenar Km 9 1 28034 Madrid, Spain
    Curr Opin Pharmacol 11:477-85. 2011
    ..Co-resistance and co-selection processes increase the opportunity for persistence of the bacteria and resistance genes and should be considered when designing strategies for decreasing antimicrobial resistance...
  18. ncbi [Interpretation of the antiobiogram for the antibiotic choice and form of administration]
    R Cantón Moreno
    Servicio de Microbiologia, Hospital Universitario Ramon y Cajal, Madrid
    Rev Clin Esp 203:608-11. 2003
  19. doi Antibiotic resistance genes from the environment: a perspective through newly identified antibiotic resistance mechanisms in the clinical setting
    R Canton
    Servicio de Microbiologia, Hospital Universitario Ramón y Cajal and Departamento de Microbiología, Facultad de Farmacia, Universidad Complutense, Madrid, Spain
    Clin Microbiol Infect 15:20-5. 2009
    ....
  20. ncbi Antimicrobial susceptibility of Gram-negative organisms from intraabdominal infections and evolution of isolates with extended spectrum β-lactamases in the SMART study in Spain (2002-2010)
    Rafael Canton
    Servicio de Microbiologia, Hospital Universitario Ramon y Cajal, Carretera de Colmenar Km 9 1, 28034 Madrid, Spain
    Rev Esp Quimioter 24:223-32. 2011
    ....
  21. ncbi Antimicrobial susceptibility profile of molecular typed cystic fibrosis Stenotrophomonas maltophilia isolates and differences with noncystic fibrosis isolates
    Rafael Canton
    Servicio de Microbiologia, Hospital Ramon y Cajal, Madrid, Spain
    Pediatr Pulmonol 35:99-107. 2003
    ..maltophilia isolates when compared with respiratory isolates from non-CF patients. Moreover, persistently recovered CF S. maltophilia isolates were more resistant than sporadic non-CF isolates...
  22. pmc Quality control for beta-lactam susceptibility testing with a well-defined collection of Enterobacteriaceae and Pseudomonas aeruginosa strains in Spain
    Rafael Canton
    Servicio de Microbiologia, Hospital Universitario Ramon y Cajal, 28034 Madrid
    J Clin Microbiol 41:1912-8. 2003
    ..A K1-producing K. oxytoca was misidentified by 10% of laboratories as an ESBL producer. The use of well-defined resistant strains is useful for improving proficiency in susceptibility testing in clinical laboratories...
  23. ncbi Prevalence and spread of extended-spectrum beta-lactamase-producing Enterobacteriaceae in Europe
    R Canton
    Servicio de Microbiologia, Hospital Universitario Ramon y Cajal, Madrid, Spain
    Clin Microbiol Infect 14:144-53. 2008
    ....
  24. ncbi Multi-resistant Gram-negative bacilli: from epidemics to endemics
    Rafael Canton
    Department of Microbiology, Ramon y Cajal University Hospital, Madrid, Spain
    Curr Opin Infect Dis 16:315-25. 2003
    ..This review focuses on recent data that may help to understand the emergence and dissemination of multi-drug resistant bacilli and the current trend from epidemic to endemic situations...
  25. ncbi IRT and CMT beta-lactamases and inhibitor resistance
    R Canton
    Servicio de Microbiologia, Hospital Universitario Ramon y Cajal, Madrid, Spain
    Clin Microbiol Infect 14:53-62. 2008
    ..CTX-M enzymes with the IRT phenotype have not been detected to date. New studies of IRT enzymes, including population structure, association with virulence traits and plasmid dispersion, are needed...
  26. ncbi Antibacterial resistance patterns in Streptococcus pneumoniae isolated from elderly patients: PROTEKT years 1-5 (1999-2004)
    Rafael Canton
    Hospital Universitario Ramon y Cajal, Servicio de Microbiologia, Carretera de Colmenar Km 9 1, 28034 Madrid, Spain
    Int J Antimicrob Agents 30:546-50. 2007
    ..Antibacterial resistance was highest in the Far East. Telithromycin resistance was rare (0.12%). Appropriate alternative empirical first-line therapies may be required for treating community-acquired RTIs in the elderly...
  27. pmc Dissemination and persistence of blaCTX-M-9 are linked to class 1 integrons containing CR1 associated with defective transposon derivatives from Tn402 located in early antibiotic resistance plasmids of IncHI2, IncP1-alpha, and IncFI groups
    Angela Novais
    Hospital Universitario Ramon y Cajal, IMSALUD, Madrid 28034, Spain
    Antimicrob Agents Chemother 50:2741-50. 2006
    ..The presence of bla(CTX-M-9) on broad-host-range IncP-1alpha plasmids might contribute to its dissemination to hosts that were not members of the family Enterobacteriaceae...
  28. doi High clonal diversity in erythromycin-resistant Streptococcus pneumoniae invasive isolates in Madrid, Spain (2000-07)
    Elia Gómez G de la Pedrosa
    Servicio de Microbiologia, Hospital Ramón y Cajal y CIBER en Epidemiología y Salud Pública CIBERESP, Madrid, Spain
    J Antimicrob Chemother 64:1165-9. 2009
    ..All 78 erythromycin-resistant S. pneumoniae isolates collected from blood cultures in our hospital (2000-07) were studied and the population structure was analysed by using different mathematical diversity indexes...
  29. doi Characterization of plasmids encoding blaESBL and surrounding genes in Spanish clinical isolates of Escherichia coli and Klebsiella pneumoniae
    Karol Diestra
    Servicio de Microbiologia, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain
    J Antimicrob Chemother 63:60-6. 2009
    ..The aim of the study was to characterize plasmids that harbour blaESBL genes and their genetic environment in Escherichia coli and Klebsiella pneumoniae clones circulating in Spain...
  30. pmc Fluoroquinolone-resistant Streptococcus pneumoniae in Spain: activities of garenoxacin against clinical isolates including strains with altered topoisomerases
    María Isabel Morosini
    Servicio de Microbiologia, Hospital Universitario Ramon y Cajal, Madrid, Spain
    Antimicrob Agents Chemother 47:2692-5. 2003
    ..5 to 1 micro g/ml) found in four isolates. The low impact of these mutations on garenoxacin activity envisages the possible coverage of S. pneumoniae populations resistant to preexisting quinolones...
  31. pmc Longer intestinal persistence of Enterococcus faecalis compared to Enterococcus faecium clones in intensive-care-unit patients
    Patricia Ruiz-Garbajosa
    Servicio de Microbiologia, Hospital Universitario Ramon y Cajal, Madrid, Spain
    J Clin Microbiol 47:345-51. 2009
    ..faecalis showed a cumulative increase in CDPD, whereas E. faecium did not. CDPD and CPR were useful for measuring the dynamics of intestinal colonization with enterococcal clones...
  32. doi Emergence of bla KPC-3-Tn4401a associated with a pKPN3/4-like plasmid within ST384 and ST388 Klebsiella pneumoniae clones in Spain
    Tânia Curiao
    Servicio de Microbiologia, Hospital Universitario Ramon y Cajal, CIBER en Epidemiología y Salud Pública CIBERESP and Instituto Ramón y Cajal de Investigación Sanitaria IRYCIS, Madrid, Spain
    J Antimicrob Chemother 65:1608-14. 2010
    ..KPC carbapenemase-producing Klebsiella pneumoniae isolates have been increasingly detected in Europe. We report the first KPC-producing isolates characterized in Madrid, Spain...
  33. pmc Antibiotic coresistance in extended-spectrum-beta-lactamase-producing Enterobacteriaceae and in vitro activity of tigecycline
    María Isabel Morosini
    Servicio de Microbiologia, Hospital Universitario Ramon y Cajal, 28034 Madrid, Spain
    Antimicrob Agents Chemother 50:2695-9. 2006
    ..However, this in vitro promise requires support from clinical studies...
  34. doi High prevalence in cystic fibrosis patients of multiresistant hospital-acquired methicillin-resistant Staphylococcus aureus ST228-SCCmecI capable of biofilm formation
    Auxiliadora Molina
    Servicio de Microbiologia, Hospital Universitario Ramón y Cajal y CIBER en Epidemiología y Salud Pública, 28034 Madrid, Spain
    J Antimicrob Chemother 62:961-7. 2008
    ..We analysed all MRSA isolates recovered from respiratory secretions of patients attending our CF unit (1994-2006)...
  35. pmc Population analysis and epidemiological features of inhibitor-resistant-TEM-beta-lactamase-producing Escherichia coli isolates from both community and hospital settings in Madrid, Spain
    Oihane Martín
    Servicio de Microbiologia, Hospital Universitario Ramon y Cajal, Madrid, Spain
    J Clin Microbiol 48:2368-72. 2010
    ..Despite this situation, clonal expansion and/or gene dispersion was not observed, denoting the independent emergence of these enzymes...
  36. pmc Global spread of the hyl(Efm) colonization-virulence gene in megaplasmids of the Enterococcus faecium CC17 polyclonal subcluster
    Ana R Freitas
    Servicio de Microbiologia, Hospital Universitario Ramon y Cajal, Carretera de Colmenar, Madrid 28034, Spain
    Antimicrob Agents Chemother 54:2660-5. 2010
    ..faecium isolates with different origins...
  37. pmc Wide dispersion of ST175 clone despite high genetic diversity of carbapenem-nonsusceptible Pseudomonas aeruginosa clinical strains in 16 Spanish hospitals
    María García-Castillo
    Servicio de Microbiologia, Hospital Universitario Ramon y Cajal, Ctra Colmenar Km 9 1, 28034 Madrid, Spain
    J Clin Microbiol 49:2905-10. 2011
    ..aeruginosa clones at high risk for epidemic spread, such as the Liverpool, Manchester, and Melbourne clones previously found in cystic fibrosis patients and ST235 in the nosocomial setting...
  38. pmc Polyclonal population structure of Streptococcus pneumoniae isolates in Spain carrying mef and mef plus erm(B)
    Elia Gómez G de la Pedrosa
    Servicio de Microbiologia, Hospital Universitario Ramon y Cajal, and CIBER ESP, Madrid, Spain
    Antimicrob Agents Chemother 52:1964-9. 2008
    ..A polyclonal population structure was demonstrated within our Spanish mef-positive S. pneumoniae isolates, with few clonal complexes overrepresented within this collection...
  39. ncbi Streptococcus pyogenes isolates with characterized macrolide resistance mechanisms in Spain: in vitro activities of telithromycin and cethromycin
    María Isabel Morosini
    Servicio de Microbiologia, Hospital Universitario Ramon y Cajal, Ctra de Colmenar, Km 9, 1, 28034 Madrid, Spain
    J Antimicrob Chemother 52:50-5. 2003
    ..According to the present results, telithromycin and cethromycin offer a wide coverage against S. pyogenes isolates in a geographic area with a high incidence of resistance to currently used macrolides...
  40. ncbi [Comparative in vitro activity of daptomycin against gram-positive microorganisms: SENTRY surveillance program, Spain (2002-2006)]
    Elena Loza
    Servicio de Microbiologia, Hospital Universitario Ramon y Cajal, Madrid, Espana
    Enferm Infecc Microbiol Clin 26:489-94. 2008
    ..The possible therapeutic role of daptomycin is addressed in the light of Spanish susceptibility data...
  41. doi Extended-spectrum beta-lactamase-producing Escherichia coli in Spain belong to a large variety of multilocus sequence typing types, including ST10 complex/A, ST23 complex/A and ST131/B2
    Jesús Oteo
    Laboratório de Antibióticos, Servicio de Bacteriologia, Centro Nacional de Microbiologia, Instituto de Salud Carlos III, Madrid, Spain
    Int J Antimicrob Agents 34:173-6. 2009
    ..coli ST10 complex carried five ESBLs and E. coli ST23 complex carried four ESBLs. A great diversity of MLST types was observed among Spanish ESBL-producing E. coli isolates...
  42. pmc Population structure and antimicrobial susceptibility of both nonpersistent and persistent Pseudomonas aeruginosa isolates recovered from cystic fibrosis patients
    Ana Fernández-Olmos
    Servicio de Microbiología and Unidad de Resistencia a Antibióticos y Virulencia Bacteriana Asociada al Consejo Superior de Investigaciones Científicas, Hospital Universitario Ramón y Cajal and Instituto Ramón y Cajal de Investigación Sanitaria IRYCIS, Madrid, Spain
    J Clin Microbiol 51:2761-5. 2013
    ..International CF epidemic clones were not identified, but the high-risk clone ST274, also found circulating in Spanish hospitals, was present. Persistent isolates were more resistant to antibiotics than nonpersistent isolates...
  43. pmc Population structure, antimicrobial resistance, and mutation frequencies of Streptococcus pneumoniae isolates from cystic fibrosis patients
    Rosa del Campo
    Servicio de Microbiología y Unidad de Fibrosis Quistica, Hospital Universitario, Ramon y Cajal, 28034 Madrid, Spain
    J Clin Microbiol 43:2207-14. 2005
    ..pneumoniae to the CF lung environment...
  44. pmc Dramatic increase in prevalence of fecal carriage of extended-spectrum beta-lactamase-producing Enterobacteriaceae during nonoutbreak situations in Spain
    Aranzazu Valverde
    Servicio de Microbiologia, Hospital Universitario Ramon y Cajal, Madrid, Spain
    J Clin Microbiol 42:4769-75. 2004
    ....
  45. doi In vitro prevention of Pseudomonas aeruginosa early biofilm formation with antibiotics used in cystic fibrosis patients
    Ana Fernández-Olmos
    Servicio de Microbiologia, and CIBER en Epidemiología y Salud Pública CIBERESP, Instituto Ramón y Cajal de Investigación Sanitaria IRYCIS and Hospital Universitario Ramón y Cajal, 28034 Madrid, Spain
    Int J Antimicrob Agents 40:173-6. 2012
    ..aeruginosa isolates recovered during early colonisation stages in CF patients supports early aggressive antimicrobial treatment guidelines in first P. aeruginosa-colonised CF patients...
  46. doi Assessment of prevalence and changing epidemiology of extended-spectrum beta-lactamase-producing Enterobacteriaceae fecal carriers using a chromogenic medium
    Raquel Paniagua
    Servicio de Microbiologia, Hospital Universitario Ramón y Cajal and CIBER en Epidemiología y Salud Pública CIBERESP, Instituto de Investigación Ramón y Cajal IRYCIS, 28034 Madrid, Spain
    Diagn Microbiol Infect Dis 67:376-9. 2010
    ..The chromID ESBL medium was reliable to screen ESBL fecal carriers with a general decrease in the laboratory workload. Time-to-time monitoring of ESBL fecal carriers is useful to ascertain current trend of ESBL epidemiology...
  47. pmc High rate of intestinal colonization with extended-spectrum-beta-lactamase-producing organisms in household contacts of infected community patients
    Aranzazu Valverde
    Servicio de Microbiologia, Hospital Universitario Ramon y Cajal, 28034 Madrid, Spain
    J Clin Microbiol 46:2796-9. 2008
    ..Patients with community infections and members of their households represent a reservoir for ESBL producers, increasing dispersal of resistance in healthy people...
  48. doi [Extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae in Spanish hospitals: 2nd multicenter study (GEIH-BLEE project, 2006)]
    Miguel Angel Díaz
    Servicio de Microbiologia, Hospital Universitario Virgen Macarena, Sevilla, Espana
    Enferm Infecc Microbiol Clin 27:503-10. 2009
    ..The 2nd nationwide study was developed in 2006 to investigate the evolution of this problem...
  49. pmc Spread of bla(CTX-M-14) is driven mainly by IncK plasmids disseminated among Escherichia coli phylogroups A, B1, and D in Spain
    Aranzazu Valverde
    Servicio de Microbiologia, Hospital Universitario Ramon y Cajal, Madrid, Spain
    Antimicrob Agents Chemother 53:5204-12. 2009
    ..The increase in the incidence of such isolates is mostly due to the dissemination of IncK plasmids among E. coli isolates of phylogroups A, B1, and D...
  50. pmc International spread and persistence of TEM-24 is caused by the confluence of highly penetrating enterobacteriaceae clones and an IncA/C2 plasmid containing Tn1696::Tn1 and IS5075-Tn21
    Angela Novais
    Servicio de Microbiologia, Hospital Universitario Ramon y Cajal, Carretera de Colmenar, Km 9 1, Madrid 28034, Spain
    Antimicrob Agents Chemother 54:825-34. 2010
    ....
  51. pmc Preservation of integron types among Enterobacteriaceae producing extended-spectrum beta-lactamases in a Spanish hospital over a 15-year period (1988 to 2003)
    Elisabete Machado
    Servicio de Microbiologia, Hospital Universitario Ramón y Cajal Carretera de Colmenar, km 9 1 Madrid 28034, Spain
    Antimicrob Agents Chemother 51:2201-4. 2007
    ..Some integrons were identical to those found during decades worldwide, illustrating the preservation of the genetic elements carrying them...
  52. pmc High genetic diversity among Stenotrophomonas maltophilia strains despite their originating at a single hospital
    Sylvia Valdezate
    Servicio de Microbiologia, Hospital Universitario Ramon y Cajal, Madrid 28034, Spain
    J Clin Microbiol 42:693-9. 2004
    ..However, few clones could be transmitted among different patients, yielding outbreak situations...
  53. pmc Breakpoints for predicting Pseudomonas aeruginosa susceptibility to inhaled tobramycin in cystic fibrosis patients: use of high-range Etest strips
    María I Morosini
    Servicio de Microbiologia, Hospital Universitario Ramon y Cajal, Madrid, Spain
    J Clin Microbiol 43:4480-5. 2005
    ..CLSI breakpoints should still be followed when intravenous tobramycin is used in CF patients, particularly during the course of exacerbations...
  54. ncbi Telithromycin activity is reduced by efflux in Streptococcus pyogenes
    Rafael Canton
    Servicio de Microbiologia, Hospital Universitario Ramon y Cajal, Carretera de Colmenar, Km 9 1, 28034 Madrid, Spain
    J Antimicrob Chemother 55:489-95. 2005
    ..To investigate whether telithromycin is a substrate for efflux pumps in Streptococcus pyogenes...
  55. pmc CTX-M-10 linked to a phage-related element is widely disseminated among Enterobacteriaceae in a Spanish hospital
    Antonio Oliver
    Servicio de Microbiologia, Hospital Son Dureta, C Andrea Doria No 55, 07014 Palma de Mallorca, Spain
    Antimicrob Agents Chemother 49:1567-71. 2005
    ..This is the first report showing an extended-spectrum-beta-lactamase gene linked to a phage-related element...
  56. ncbi Differences in biofilm development and antibiotic susceptibility among Streptococcus pneumoniae isolates from cystic fibrosis samples and blood cultures
    María García-Castillo
    Servicio de Microbiologia, Hospital Universitario Ramón y Cajal Ctra Colmenar Km 9 1, Madrid 28034, Spain
    J Antimicrob Chemother 59:301-4. 2007
    ....
  57. pmc Mutational events in cefotaximase extended-spectrum beta-lactamases of the CTX-M-1 cluster involved in ceftazidime resistance
    Angela Novais
    Hospital Universitario Ramon y Cajal, IMSALUD, Madrid, Spain
    Antimicrob Agents Chemother 52:2377-82. 2008
    ..This suggests that in this context this mutation might be considered a primary site involved in resistance to broad-spectrum cephalosporins...
  58. pmc Emergence of a mutL mutation causing multilocus sequence typing-pulsed-field gel electrophoresis discrepancy among Pseudomonas aeruginosa isolates from a cystic fibrosis patient
    María García-Castillo
    Servicio de Microbiología and CIBER en Epidemiología y Salud Pública, Instituto Ramón y Cajal de Investigación Sanitaria, Hospital Universitario Ramon y Cajal, Madrid, Spain
    J Clin Microbiol 50:1777-8. 2012
    ....
  59. pmc In vitro selection of variants resistant to beta-lactams plus beta-lactamase inhibitors in CTX-M beta-lactamases: predicting the in vivo scenario?
    Aida Ripoll
    Servicio de Microbiología and CIBER en Epidemiología y Salud Pública CIBERESP, Instituto Ramón y Cajal de Investigación Sanitaria IRYCIS, Madrid, Spain
    Antimicrob Agents Chemother 55:4530-6. 2011
    ..In summary, IR-CTX-M enzymes might evolve under exposure to BBLIs, and the probability is higher for enzymes belonging to the CTX-M-1 cluster. However, this process could be delayed by antagonistic pleiotropy...
  60. ncbi The CTX-M beta-lactamase pandemic
    Rafael Canton
    Servicio de Microbiologia, Hospital Ramon y Cajal, 28034 Madrid, Spain
    Curr Opin Microbiol 9:466-75. 2006
    ..All these events might have contributed to the current pandemic CTX-M beta-lactamase scenario...
  61. pmc Population structure of Enterococcus faecium causing bacteremia in a Spanish university hospital: setting the scene for a future increase in vancomycin resistance?
    Teresa M Coque
    Departamento de Microbiologíca, Servicio de Microbiologia, Hospital Universitario Ramon y Cajal, Carretera de Colmenar, Km 9 1, Madrid 28034, Spain
    Antimicrob Agents Chemother 49:2693-700. 2005
    ..001). These findings reflect the importance of local environmental differences in the evolution of this CC, suggesting that the emergence of vancomycin resistance among AREF strains in Spain may be a question of time...
  62. pmc Evolutionary trajectories of beta-lactamase CTX-M-1 cluster enzymes: predicting antibiotic resistance
    Angela Novais
    Hospital Universitario Ramon y Cajal, IMSALUD, Madrid, Spain
    PLoS Pathog 6:e1000735. 2010
    ....
  63. ncbi Complex molecular epidemiology of extended-spectrum beta-lactamases in Klebsiella pneumoniae: a long-term perspective from a single institution in Madrid
    Aranzazu Valverde
    Servicio de Microbiologia, Hospital Universitario Ramón y Cajal and CIBER ESP, Madrid, Spain
    J Antimicrob Chemother 61:64-72. 2008
    ..To analyse all extended-spectrum-beta-lactamase (ESBL)-producing Klebsiella pneumoniae isolates recovered from 2001 to 2004 in our institution and to compare this period with that of 1989 to 2000...
  64. ncbi Use of tigecycline for the treatment of prolonged bacteremia due to a multiresistant VIM-1 and SHV-12 beta--lactamase-producing Klebsiella pneumoniae epidemic clone
    Javier Cobo
    Servicio de Enfermedades Infecciosas, Hospital Universitario Ramon y Cajal, 28034 Madrid, Spain
    Diagn Microbiol Infect Dis 60:319-22. 2008
    ..Time-kill studies demonstrated that the combination of both compounds was synergistic along the first 12 h, suppressing the regrowth observed after 3 to 6 h when colistin was tested alone...
  65. pmc Epidemiology of extended-spectrum beta-lactamase-producing Enterobacter isolates in a Spanish hospital during a 12-year period
    Rafael Canton
    Servicio de Microbiología Hospital Ramón y Cajal, Madrid, Spain
    J Clin Microbiol 40:1237-43. 2002
    ..The presence of the TEM-24-producing E. aerogenes French clone in our institution also demonstrates the intercountry dissemination of ESBL-producing isolates...
  66. pmc Dynamics of long-term colonization of respiratory tract by Haemophilus influenzae in cystic fibrosis patients shows a marked increase in hypermutable strains
    Federico Román
    Centro Nacional de Microbiologia, Instituto de Salud Carlos III, Majadahonda Department of Microbiology, Hospital Universitario Ramon y Cajal, Madrid, Spain
    J Clin Microbiol 42:1450-9. 2004
    ..These results suggest that H. influenzae bronchial colonization in CF patients is a dynamic process, but better-adapted clones can persist for long periods of time...
  67. pmc Laboratory detection of Haemophilus influenzae with decreased susceptibility to nalidixic acid, ciprofloxacin, levofloxacin, and moxifloxacin due to GyrA and ParC mutations
    María Pérez-Vázquez
    Centro Nacional de Microbiologia, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain
    J Clin Microbiol 42:1185-91. 2004
    ..influenzae isolates for the detection of decreased susceptibilities to quinolones due to the acquisition of two amino acid changes in the QRDRs of GyrA and ParC (sensitivity, >95%; specificity, >80%)...
  68. pmc Dissemination of clonally related Escherichia coli strains expressing extended-spectrum beta-lactamase CTX-M-15
    Teresa M Coque
    Hospital Universitario Ramon y Cajal, Madrid, Spain
    Emerg Infect Dis 14:195-200. 2008
    ..Most plasmids contained blaOXA-1, aac(6')-Ib-cr, and blaTEM-1. Worldwide dissemination of CTX-M-15 seems to be determined by clonal complexes ST131 and ST405 and multidrug-resistant IncFII plasmids...
  69. doi [Microbiological diagnosis of bronchopulmonary colonization-infection in cystic fibrosis]
    Antonio Oliver
    Servicio de Microbiologia, Hospital Son Dureta, Palma de Mallorca, Espana
    Enferm Infecc Microbiol Clin 27:89-104. 2009
    ....
  70. pmc Dispersal of carbapenemase blaVIM-1 gene associated with different Tn402 variants, mercury transposons, and conjugative plasmids in Enterobacteriaceae and Pseudomonas aeruginosa
    Marta Tato
    Servicio de Microbiologia, Hospital Universitario Ramon y Cajal, Carretera de Colmenar, Km 9 1, Madrid 28034, Spain
    Antimicrob Agents Chemother 54:320-7. 2010
    ..Knowledge of the complex epidemiology of bla(VIM-1) is necessary to control this emerging threat...
  71. pmc Association of composite IS26-sul3 elements with highly transmissible IncI1 plasmids in extended-spectrum-beta-lactamase-producing Escherichia coli clones from humans
    Tânia Curiao
    Servicio de Microbiologia, IRYCIS, Madrid, Spain
    Antimicrob Agents Chemother 55:2451-7. 2011
    ....
  72. pmc Multilocus sequence typing scheme for Enterococcus faecalis reveals hospital-adapted genetic complexes in a background of high rates of recombination
    Patricia Ruiz-Garbajosa
    Servicio de Microbiologia, Hospital Universitario Ramon y Cajal, Madrid, Spain
    J Clin Microbiol 44:2220-8. 2006
    ..faecalis. Our novel MLST scheme provides an excellent tool for investigating local and short-term epidemiology as well as global epidemiology, population structure, and genetic evolution of E. faecalis...
  73. pmc Contribution of IncFII and broad-host IncA/C and IncN plasmids to the local expansion and diversification of phylogroup B2 Escherichia coli ST131 clones carrying blaCTX-M-15 and qnrS1 genes
    Angela Novais
    Servicio de Microbiología and CIBER en Epidemiología y Salud Pública, Instituto Ramón y Cajal de Investigación Sanitaria and Hospital Universitario Ramón y Cajal, Madrid, Spain
    Antimicrob Agents Chemother 56:2763-6. 2012
    ....
  74. pmc In117, an unusual In0-like class 1 integron containing CR1 and bla(CTX-M-2) and associated with a Tn21-like element
    Aranzazu Valverde
    Hospital Universitario Ramon y Cajal, IMSALUD, Madrid, Spain
    Antimicrob Agents Chemother 50:799-802. 2006
    ..This element constitutes one of the few examples of CR1-bearing class 1 integrons that has been fully characterized...
  75. ncbi Combinations of PBPs and MurM protein variants in early and contemporary high-level penicillin-resistant Streptococcus pneumoniae isolates in Spain
    Rosa del Campo
    Servicio de Microbiologia, Hospital Universitario Ramon y Cajal, Ctra Colmenar Km 9 1, Madrid 28034, Spain
    J Antimicrob Chemother 57:983-6. 2006
    ..In this work, we determined whether specific changes in PBPs were associated with specific MurM variants...
  76. pmc Polymorphic mutation frequencies of clinical and environmental Stenotrophomonas maltophilia populations
    María Carmen Turrientes
    Servicio de Microbiologia, Hospital Universitario Ramón y Cajal and Unidad Asociada al CSIC Resistencia a los Antibióticos y Virulencia Bacteriana, Madrid, Spain
    Appl Environ Microbiol 76:1746-58. 2010
    ..In silico analysis of the amino acid changes present in the MutS proteins of these hypermutator strains in comparison with the normomutator isolates suggests that the cause of the defect in MutS might be a H683P amino acid change...
  77. ncbi [Characterization and molecular epidemiology of ESBL in Escherichia coli and Klebsiella pneumoniae in 11 Spanish hospitals (2004)]
    Karol Diestra
    Servicio de Microbiologı a, Hospital de la Santa Creu i Sant Pau, Departament de Gen etica i Microbiologia, Universitat Autonoma de Barcelona, Bellaterra, Cerdanyola del Valles, Barcelona, Spain
    Enferm Infecc Microbiol Clin 26:404-10. 2008
    ..The epidemiological distribution of extended-spectrum beta-lactamase (ESBL) types in Escherichia coli and Klebsiella pneumoniae was evaluated in various hospitals in Spain and compared with previous studies...
  78. pmc Reidentification of Streptococcus bovis isolates causing bacteremia according to the new taxonomy criteria: still an issue?
    Beatriz Romero
    Servicio de Microbiologia, Hospital Universitario Ramon y Cajal, Ctra Colmenar, Km 9, 1, 28034 Madrid, Spain
    J Clin Microbiol 49:3228-33. 2011
    ..We conclude that sequencing of the sodA gene was the most discriminatory method and that S. gallolyticus subsp. pasteurianus appears to have a higher genetic diversity than S. gallolyticus subsp. gallolyticus...
  79. pmc Emergence and dissemination of Enterobacteriaceae isolates producing CTX-M-1-like enzymes in Spain are associated with IncFII (CTX-M-15) and broad-host-range (CTX-M-1, -3, and -32) plasmids
    Angela Novais
    Servicio de Microbiologia, Hospital Universitario Ramon y Cajal, IMSALUD, Carretera de Colmenar, Km 9 1, Madrid 28034, Spain
    Antimicrob Agents Chemother 51:796-9. 2007
    ..The identical genetic surroundings of blaCTX-M-32 and blaCTX-M-1 and their locations on related 40-kb IncN plasmids indicate the in vivo evolution of this element...
  80. ncbi [Antimicrobial use in Spanish hospitals (EPINE, 1990-1999)]
    Angel Asensio
    Hospital Universitario Puerta de Hierro, Madrid, Spain
    Med Clin (Barc) 118:731-6. 2002
    ..Monitoring of antimicrobial use and knowledge of prescription habits are some of the strategies recommended to contain the resistance to antimicrobials in hospitals...
  81. pmc Genes encoding TEM-4, SHV-2, and CTX-M-10 extended-spectrum beta-lactamases are carried by multiple Klebsiella pneumoniae clones in a single hospital (Madrid, 1989 to 2000)
    Teresa M Coque
    Servicio de Microbiologia, Hospital Ramon y Cajal, Madrid, Spain
    Antimicrob Agents Chemother 46:500-10. 2002
    ....
  82. doi [Tolerance and heteroresistance in Gram-positive microorganisms]
    María Isabel Morosini
    Servicio de Microbiología y CIBER de Investigación en Salud Pública, Instituto Ramón y Cajal de Investigación Sanitaria IRYCIS, Hospital Universitario Ramon y Cajal, Madrid, Espana
    Med Clin (Barc) 135:16-22. 2010
    ..New antimicrobials, including daptomycin, are less affected by these phenomena and should be considered as the treatment of choice when these events are demonstrated or suspected...
  83. doi Unorthodox long-term aerosolized ampicillin use for methicillin-susceptible Staphylococcus aureus lung infection in a cystic fibrosis patient
    Luis Maiz
    Cystic Fibrosis Unit, Department of Pulmonology, Hospital Ramon y Cajal, Madrid, Spain
    Pediatr Pulmonol 44:512-5. 2009
    ..We report a successful long-term aerosolized ampicillin treatment of a 14-year-old girl with chronic symptomatic S. aureus lung infection...
  84. ncbi [Recommendations for selecting antimicrobial agents for in vitro susceptibility studies using automatic and semiautomatic systems]
    Rafael Canton
    Servicio de Microbiologia, Hospital Universitario Ramon y Cajal, Madrid, Espana
    Enferm Infecc Microbiol Clin 25:394-400. 2007
    ..This approach will lead to more accurate susceptibility testing results with better detection of resistance mechanisms, and allowing to reach the clinical goal of the antibiogram...
  85. ncbi Antimicrobial resistance amongst isolates of Streptococcus pyogenes and Staphylococcus aureus in the PROTEKT antimicrobial surveillance programme during 1999-2000
    Rafael Canton
    Servicio de Microbiologia, Hospital Universitario Ramon y Cajal, Ctra Colmenar, Km 9, 100, 28034 Madrid, Spain
    J Antimicrob Chemother 50:9-24. 2002
    ..06-0.25 mg/L) in Australia, Indonesia, Hungary, Austria, Germany, the Netherlands, Portugal, Sweden and Switzerland. Regardless of methicillin susceptibility, resistance to linezolid, teicoplanin or vancomycin was not apparent globally...
  86. doi Antibiotics and antibiotic resistance in water environments
    Fernando Baquero
    Department of Microbiology, Ramon y Cajal University Hospital, CIBER ESP, Spain
    Curr Opin Biotechnol 19:260-5. 2008
    ..A policy for preventing mixing human-originated and animal-originated bacteria with environmental organisms seems advisable...
  87. ncbi [Prevalence of infection by carbapenem-resistant Acinetobacter baumannii in Spain (1999-2005)]
    Angel Asensio
    Servicio de Medicina Preventiva, Hospital Universitario Puerta de Hierro, Madrid, Espana
    Enferm Infecc Microbiol Clin 26:199-204. 2008
    ..This study determines the prevalence of infections by carbapenem-resistant Acinetobacter baumannii (CRAB) in Spain, and identifies trends over time, geographical variations, and factors associated with resistance...
  88. ncbi High occurrence of esp among ampicillin-resistant and vancomycin-susceptible Enterococcus faecium clones from hospitalized patients
    Teresa M Coque
    Servicio de Microbiologia, Hospital Ramon y Cajal, Carretera de Colmenar, Km 9 1, Madrid 28034, Spain
    J Antimicrob Chemother 50:1035-8. 2002
    ..faecium clones. This observation indicates that antibiotic-resistant variants may frequently arise under antibiotic selective pressure among esp-positive clones reaching ecological abundance in the nosocomial habitat...
  89. ncbi [High-risk clonal complexes CC2 and CC9 are widely distributed among Enterococcus faecalis hospital isolates recovered in Spain]
    Patricia Ruiz-Garbajosa
    Servicio de Microbiología Hospital Universitario Ramón y Cajal Madrid España
    Enferm Infecc Microbiol Clin 25:513-8. 2007
    ..The purpose of this study was to define the presence of CC2 and CC9 among E. faecalis strains isolated in Spain...
  90. ncbi [Comparative in vitro activity of garenoxacin (BMS-284756). Sentry program, Spain (1999-2000)]
    Elena Loza
    Servicio de Microbiología Hospital Ramón y Cajal Madrid Spain
    Enferm Infecc Microbiol Clin 21:404-9. 2003
    ....
  91. ncbi Evaluation of 4 swab transport systems for the recovery of ATCC and clinical strains with characterized resistance mechanisms
    María Isabel Morosini
    Servicio de Microbiologia, Hospital Universitario Ramon y Cajal, 28034 Madrid, Spain
    Diagn Microbiol Infect Dis 56:19-24. 2006
    ..Resistance mechanisms in clinical isolates do not affect survival with any of the STS...
  92. pmc Dissemination of blaKPC-2 by the spread of Klebsiella pneumoniae clonal complex 258 clones (ST258, ST11, ST437) and plasmids (IncFII, IncN, IncL/M) among Enterobacteriaceae species in Brazil
    Leonardo Neves Andrade
    Faculdade de Ciencias Farmaceuticas de Ribeirao Preto, Universidade de Sao Paulo, Ribeirao Preto, Brazil
    Antimicrob Agents Chemother 55:3579-83. 2011
    ..It also constitutes the first description of sequence type 258 (ST258) in Brazil, which was associated with a nosocomial hospital outbreak in Ribeirao Preto city...
  93. ncbi Preservation of topoisomerase genetic sequences during in vivo and in vitro development of high-level resistance to ciprofloxacin in isogenic Stenotrophomonas maltophilia strains
    Sylvia Valdezate
    Departamento de Bacteriologia, Centro Nacional de Microbiologia, Instituto Carlos III, Majadahonda, Madrid, Spain
    J Antimicrob Chemother 56:220-3. 2005
    ..To ascertain the participation of topoisomerase mutations in the development of ciprofloxacin resistance in isogenic Stenotrophomonas maltophilia mutants...
  94. ncbi Respiratory tract infections: at-risk patients, who are they? Implications for their management with levofloxacin
    Rafael Canton
    Servicio de Microbiologia, Hospital Universitario Ramon y Cajal, Madrid, Spain
    Int J Antimicrob Agents 28:S115-27. 2006
    ....
  95. ncbi [Etiology of hospital-acquired infections in Spanish hospitals (EPINE, 1990-1999)]
    Angel Asensio
    Hospital Universitario Puerta de Hierro, Madrid, Spain
    Med Clin (Barc) 118:725-30. 2002
    ..Estimate of prevalence of infection was calculated by means of the percent distribution of every organism regarding overall identified organisms and infections...