J J Calvete

Summary

Country: Spain

Publications

  1. ncbi request reprint Venom proteomes of closely related Sistrurus rattlesnakes with divergent diets
    Libia Sanz
    Instituto de Biomedicina de Valencia, C S I C, Jaume Roig 11, 46010 Valencia, Spain
    J Proteome Res 5:2098-112. 2006
  2. pmc Interchain and intrachain disulphide bonds in human platelet glycoprotein IIb. Localization of the epitopes for several monoclonal antibodies
    J J Calvete
    Instituto de Quimica Fisica, Consejo Superior de Investigaciones Cientificas, Serrano, Madrid, Spain
    Biochem J 261:551-60. 1989
  3. ncbi request reprint Crystal structures of Cratylia floribunda seed lectin at acidic and basic pHs. Insights into the structural basis of the pH-dependent dimer-tetramer transition
    Francisca Gallego Del Sol
    Instituto de Biomedicina de Valencia, C S I C, E 46010 Valencia, Spain
    J Struct Biol 158:1-9. 2007
  4. ncbi request reprint Proteomic profiling of a snake venom using high mass detection MALDI-TOF mass spectrometry
    Oscar Yanes
    Institut de Biotecnologia i Biomedicina and Departament de Bioquimica i Biol Mol, Universitat Autonoma de Barcelona, Bellaterra Barcelona, Spain
    J Am Soc Mass Spectrom 18:600-6. 2007
  5. ncbi request reprint Snake venomics of Bitis gabonica gabonica. Protein family composition, subunit organization of venom toxins, and characterization of dimeric disintegrins bitisgabonin-1 and bitisgabonin-2
    Juan J Calvete
    Instituto de Biomedicina de Valencia, C S I C, Jaume Roig 11, 46010 Valencia, Spain
    J Proteome Res 6:326-36. 2007
  6. ncbi request reprint Next-generation snake venomics: protein-locus resolution through venom proteome decomplexation
    Juan J Calvete
    Instituto de Biomedicina de Valencia, Consejo Superior de Investigaciones Cientificas, Jaime Roig 11, 46010 Valencia, Spain 34 963 391 778 34 963 690 800
    Expert Rev Proteomics 11:315-29. 2014
  7. doi request reprint Snake venomics of Macrovipera mauritanica from Morocco, and assessment of the para-specific immunoreactivity of an experimental monospecific and a commercial antivenoms
    Bouchra Makran
    Venoms and Toxins Laboratory, Pasteur Institute of Morocco, 1 Place Louis Pasteur, 20100 Casablanca, Morocco
    J Proteomics 75:2431-41. 2012
  8. doi request reprint The 752delG26 mutation in the RFXANK gene associated with major histocompatibility complex class II deficiency: evidence for a founder effect in the Moroccan population
    Hamid Naamane
    Laboratory of Immunology and Molecular Biology, Pasteur Institute, Casablanca, Morocco
    Eur J Pediatr 169:1069-74. 2010
  9. ncbi request reprint Snake venomics: from the inventory of toxins to biology
    Juan J Calvete
    Venomics and Structural Proteomics Laboratory, Instituto de Biomedicina de Valencia, CSIC, Jaume Roig 11, 46010 Valencia, Spain Electronic address
    Toxicon 75:44-62. 2013
  10. pmc Integrated "omics" profiling indicates that miRNAs are modulators of the ontogenetic venom composition shift in the Central American rattlesnake, Crotalus simus simus
    Jordi Durbán
    Instituto de Biomedicina de Valencia, Consejo Superior de Investigaciones Cientificas, Jaime Roig 11, Valencia 46010, Spain
    BMC Genomics 14:234. 2013

Collaborators

Detail Information

Publications93

  1. ncbi request reprint Venom proteomes of closely related Sistrurus rattlesnakes with divergent diets
    Libia Sanz
    Instituto de Biomedicina de Valencia, C S I C, Jaume Roig 11, 46010 Valencia, Spain
    J Proteome Res 5:2098-112. 2006
    ....
  2. pmc Interchain and intrachain disulphide bonds in human platelet glycoprotein IIb. Localization of the epitopes for several monoclonal antibodies
    J J Calvete
    Instituto de Quimica Fisica, Consejo Superior de Investigaciones Cientificas, Serrano, Madrid, Spain
    Biochem J 261:551-60. 1989
    ..Biol. Chem. 262, 8476-8482]...
  3. ncbi request reprint Crystal structures of Cratylia floribunda seed lectin at acidic and basic pHs. Insights into the structural basis of the pH-dependent dimer-tetramer transition
    Francisca Gallego Del Sol
    Instituto de Biomedicina de Valencia, C S I C, E 46010 Valencia, Spain
    J Struct Biol 158:1-9. 2007
    ..Our data provide new insights into the participation of specific amino acid residues in the mechanism of the quaternary association of Diocleinae lectins...
  4. ncbi request reprint Proteomic profiling of a snake venom using high mass detection MALDI-TOF mass spectrometry
    Oscar Yanes
    Institut de Biotecnologia i Biomedicina and Departament de Bioquimica i Biol Mol, Universitat Autonoma de Barcelona, Bellaterra Barcelona, Spain
    J Am Soc Mass Spectrom 18:600-6. 2007
    ..Furthermore, the sensitivity reached with the high mass cryodetection MS technology clearly exceeds the detection limit of standard high-sensitivity staining methods...
  5. ncbi request reprint Snake venomics of Bitis gabonica gabonica. Protein family composition, subunit organization of venom toxins, and characterization of dimeric disintegrins bitisgabonin-1 and bitisgabonin-2
    Juan J Calvete
    Instituto de Biomedicina de Valencia, C S I C, Jaume Roig 11, 46010 Valencia, Spain
    J Proteome Res 6:326-36. 2007
    ..Cell adhesion inhibition assays showed that bitisgabonin-1 (RGD-RGD) is a potent inhibitor of integrin alpha5beta1, whereas bitisgabonin-2 (MLD-RGD) is a better antagonist of integrins alpha4beta1 and alpha9beta1...
  6. ncbi request reprint Next-generation snake venomics: protein-locus resolution through venom proteome decomplexation
    Juan J Calvete
    Instituto de Biomedicina de Valencia, Consejo Superior de Investigaciones Cientificas, Jaime Roig 11, 46010 Valencia, Spain 34 963 391 778 34 963 690 800
    Expert Rev Proteomics 11:315-29. 2014
    ..Understanding evolutionary trends across venoms represents the Rosetta Stone for generating broad-ranging polyspecific antivenoms. ..
  7. doi request reprint Snake venomics of Macrovipera mauritanica from Morocco, and assessment of the para-specific immunoreactivity of an experimental monospecific and a commercial antivenoms
    Bouchra Makran
    Venoms and Toxins Laboratory, Pasteur Institute of Morocco, 1 Place Louis Pasteur, 20100 Casablanca, Morocco
    J Proteomics 75:2431-41. 2012
    ..Both antivenoms very efficiently immunodepleted homologous venom toxins and displayed a high degree of paraspecificity, suggesting the clinical utility of the two antivenoms for treating bites of both M. mauritanica or M. lebetina...
  8. doi request reprint The 752delG26 mutation in the RFXANK gene associated with major histocompatibility complex class II deficiency: evidence for a founder effect in the Moroccan population
    Hamid Naamane
    Laboratory of Immunology and Molecular Biology, Pasteur Institute, Casablanca, Morocco
    Eur J Pediatr 169:1069-74. 2010
    ..These findings should facilitate the establishment of molecular diagnosis and improve genetic counselling for affected Moroccan families...
  9. ncbi request reprint Snake venomics: from the inventory of toxins to biology
    Juan J Calvete
    Venomics and Structural Proteomics Laboratory, Instituto de Biomedicina de Valencia, CSIC, Jaume Roig 11, 46010 Valencia, Spain Electronic address
    Toxicon 75:44-62. 2013
    ..Selected applications of venomics and antivenomics for exploring the chemical space and immunological profile of venoms will illustrate the author's views on the impact these proteomics tools may have in the field of toxinology...
  10. pmc Integrated "omics" profiling indicates that miRNAs are modulators of the ontogenetic venom composition shift in the Central American rattlesnake, Crotalus simus simus
    Jordi Durbán
    Instituto de Biomedicina de Valencia, Consejo Superior de Investigaciones Cientificas, Jaime Roig 11, Valencia 46010, Spain
    BMC Genomics 14:234. 2013
    ..Here we have explored the basis and level of regulation of the ontogenetic shift in the venom composition of the Central American rattlesnake, Crotalus s. simus using a combined proteomics and transcriptomics approach...
  11. doi request reprint The continuing saga of snake venom disintegrins
    Juan J Calvete
    Instituto de Biomedicina de Valencia, CSIC, Jaime Roig 11, 46010 Valencia, Spain
    Toxicon 62:40-9. 2013
    ..Completing the continuing story of the disintegrin family by applying novel research approaches may hold the key to learn how to use deadly toxins as therapeutic agents...
  12. doi request reprint Snake venomics of two poorly known Hydrophiinae: Comparative proteomics of the venoms of terrestrial Toxicocalamus longissimus and marine Hydrophis cyanocinctus
    Juan J Calvete
    Consejo Superior de Investigaciones Cientificas, Valencia, Spain
    J Proteomics 75:4091-101. 2012
    ....
  13. pmc Snake venomics of Crotalus tigris: the minimalist toxin arsenal of the deadliest Neartic rattlesnake venom. Evolutionary Clues for generating a pan-specific antivenom against crotalid type II venoms
    Juan J Calvete
    Departamento de Biotecnologia, Universidad Politecnica de Valencia, Valencia, Spain
    J Proteome Res 11:1382-90. 2012
    ....
  14. pmc Profiling the venom gland transcriptomes of Costa Rican snakes by 454 pyrosequencing
    Jordi Durbán
    Consejo Superior de Investigaciones Cientificas, Jaime Roig, Valencia, Spain
    BMC Genomics 12:259. 2011
    ..In the absence of genome sequence, transcriptomes represent also valuable searchable databases for proteomic projects...
  15. ncbi request reprint Insights into structure-function correlations of ungulate seminal plasma proteins
    Juan J Calvete
    Instituto de Biomedicina de Valencia CSIC, Jaime Roig 11, 46010 Valencia, Spain
    Soc Reprod Fertil Suppl 65:201-15. 2007
    ....
  16. ncbi request reprint Snake venomics. Strategy and applications
    Juan J Calvete
    Instituto de Biomedicina de Valencia, CSIC, Jaume Roig 11, 46010 Valencia, Spain
    J Mass Spectrom 42:1405-14. 2007
    ..The combined strategy allows us to assign unambiguously all the isolated venom toxins representing over 0.05% of the total venom proteins to known protein families. Protocols and applications of snake venomics are reviewed and discussed...
  17. ncbi request reprint Snake venomics of Bitis species reveals large intragenus venom toxin composition variation: application to taxonomy of congeneric taxa
    Juan J Calvete
    Instituto de Biomedicina de Valencia, C S I C, Jaume Roig 11, 46010 Valencia, Spain
    J Proteome Res 6:2732-45. 2007
    ..g. rhinoceros as a separate species, Bitis rhinoceros. Moreover, our proteomic data fit better to a weighted phylogram based on overall genetic distances than to an unweighted maximum-parsimony tree...
  18. ncbi request reprint Snake venom disintegrins: evolution of structure and function
    Juan J Calvete
    Instituto de Biomedicina de Valencia, C S I C, Jaume Roig 11, 46010 Valencia, Spain
    Toxicon 45:1063-74. 2005
    ..The role of the composition, conformation, and dynamics of the integrin inhibitory loop acting in concert with the C-terminal tail in determining the selective inhibition of integrin receptors is discussed...
  19. ncbi request reprint Structure-function correlations of snake venom disintegrins
    Juan J Calvete
    Instituto de Biomedicina de Valencia, C S I C, Valencia, Spain
    Curr Pharm Des 11:829-35. 2005
    ..The role of the composition, conformation and concerted dynamics of the integrin inhibitory loop and the C-terminal tail in determining the selective inhibition of integrin receptors is discussed...
  20. ncbi request reprint KTS and RTS-disintegrins: anti-angiogenic viper venom peptides specifically targeting the alpha 1 beta 1 integrin
    Juan J Calvete
    Instituto de Biomedicina de Valencia, C S I C, Jaime Roig 11, 46010 Valencia, Spain
    Curr Pharm Des 13:2853-9. 2007
    ..Here we discuss structure-function correlations of the novel group of specific (K/R)TS-disintegrins targeting the alpha(1)beta(1) integrin...
  21. doi request reprint Meeting report MPSA 2007
    Juan J Calvete
    Instituto de Biomedicina de Valencia, CSIC, Jaime Roig 11, 46010 Valencia, Spain
    J Proteomics 71:4-10. 2008
  22. doi request reprint Venoms, venomics, antivenomics
    Juan J Calvete
    Instituto de Biomedicina de Valencia, C S I C, Jaume Roig 11, 46010 Valencia, Spain
    FEBS Lett 583:1736-43. 2009
    ..Further, the high degree of target specificity makes toxins valuable scaffolds for drug development...
  23. doi request reprint Exploring the venom proteome of the western diamondback rattlesnake, Crotalus atrox, via snake venomics and combinatorial peptide ligand library approaches
    Juan J Calvete
    Instituto de Biomedicina de Valencia, Consejo Superior de Investigaciones Cientificas CSIC, Jaume Roig 11, 46010 Valencia, Spain
    J Proteome Res 8:3055-67. 2009
    ....
  24. doi request reprint Snake venomics and antivenomics of Bothrops colombiensis, a medically important pitviper of the Bothrops atrox-asper complex endemic to Venezuela: Contributing to its taxonomy and snakebite management
    Juan J Calvete
    Instituto de Biomedicina de Valencia, C S I C, 46010 Valencia, Spain
    J Proteomics 72:227-40. 2009
    ..colombiensis and B. asper venom components, most notably medium-size disintegrins, bradykinin-potentiating peptides, PLA(2) proteins, and PI Zn(2+)-metalloproteinases...
  25. doi request reprint Antivenomics and venom phenotyping: A marriage of convenience to address the performance and range of clinical use of antivenoms
    Juan J Calvete
    Laboratorio de Proteinómica Estructural, Instituto de Biomedicina de Valencia, CSIC, Jaime Roig 11, 46010 Valencia, Spain
    Toxicon 56:1284-91. 2010
    ..Recent work showing how the knowledge of evolutionary trends along with venom phenotyping may have an impact in designing a mixture of venoms for immunization aimed to produce a pan-American anti-crotalic antivenom is discussed...
  26. doi request reprint Snake population venomics and antivenomics of Bothrops atrox: Paedomorphism along its transamazonian dispersal and implications of geographic venom variability on snakebite management
    Juan J Calvete
    Instituto de Biomedicina de Valencia, C S I C, Jaume Roig 11, 46010 Valencia, Spain
    J Proteomics 74:510-27. 2011
    ..The degree of immunodepletion achieved suggests that each of these antivenoms may be effective against envenomations by paedomorphic, and some ontogenetic, B. atrox venoms...
  27. doi request reprint Proteomic tools against the neglected pathology of snake bite envenoming
    Juan J Calvete
    Instituto de Biomedicina de Valencia, Consejo Superior de Investigaciones Cientificas, Jaime Roig 11, 46010 Valencia, Spain
    Expert Rev Proteomics 8:739-58. 2011
    ..Antivenomics and venom phenotyping may also contribute to expand the clinical range of currently existing antidotes...
  28. ncbi request reprint Structures of integrin domains and concerted conformational changes in the bidirectional signaling mechanism of alphaIIbbeta3
    Juan J Calvete
    Instituto de Biomedicina de Valencia, C S I C, 46010, Valencia, Spain
    Exp Biol Med (Maywood) 229:732-44. 2004
    ....
  29. pmc Snake venom disintegrins: novel dimeric disintegrins and structural diversification by disulphide bond engineering
    Juan J Calvete
    Instituto de Biomedicina de Valencia, Consejo Superior de Investigaciones Cientificas, Jaime Roig 11, Spain
    Biochem J 372:725-34. 2003
    ....
  30. ncbi request reprint The amino acid sequence of the agglutinin isolated from the red marine alga Bryothamnion triquetrum defines a novel lectin structure
    J J Calvete
    Instituto de Biomedicina de Valencia, Spain
    Cell Mol Life Sci 57:343-50. 2000
    ..The primary structure of the B. triquetrum lectin does not show amino acid sequence similarity with known plant and animal lectin structures. Hence, this protein may be the paradigm of a novel lectin family...
  31. pmc Antivenomic assessment of the immunological reactivity of EchiTAb-Plus-ICP, an antivenom for the treatment of snakebite envenoming in sub-Saharan Africa
    Juan J Calvete
    Instituto de Biomedicina de Valencia, C S I C, Jaume Roig 11, 46010 Valencia, Spain
    Am J Trop Med Hyg 82:1194-201. 2010
    ....
  32. pmc The disulfide bond pattern of catrocollastatin C, a disintegrin-like/cysteine-rich protein isolated from Crotalus atrox venom
    J J Calvete
    Instituto de Biomedicina, C S I C, Valencia, Spain
    Protein Sci 9:1365-73. 2000
    ..These results provide structural evidence for a redefinition of the disintegrin-like and cysteine-rich domain boundaries. An evolutionary pathway for ADAMs, PIII, and PII SVMPs based on disulfide bond engineering is also proposed...
  33. pmc Disulphide-bond pattern and molecular modelling of the dimeric disintegrin EMF-10, a potent and selective integrin alpha5beta1 antagonist from Eristocophis macmahoni venom
    J J Calvete
    Instituto de Biomedicina de Valencia, C S I C, Jaime Roig 11, E 46010 Valencia, Spain
    Biochem J 345:573-81. 2000
    ..In the EMF-10 model the N-terminal polypeptide of EMF-10B is close to the RGD-loop of the EMF-10A subunit, suggesting that the N-terminal region of the B-subunit could potentially influence the biological activity of the A-subunit...
  34. ncbi request reprint Platelet integrin GPIIb/IIIa: structure-function correlations. An update and lessons from other integrins
    J J Calvete
    Instituto de Biomedicina de Valencia, CSIC, Spain
    Proc Soc Exp Biol Med 222:29-38. 1999
    ....
  35. ncbi request reprint Characterisation of the conformational and quaternary structure-dependent heparin-binding region of bovine seminal plasma protein PDC-109
    J J Calvete
    Instituto de Biomedicina de Valencia, C S I C, Spain
    FEBS Lett 444:260-4. 1999
    ..In full-length PDC-109, Arg64 and Lys68 are both located in the intervening polypeptide between domains a and b. Our data suggest possible quaternary structure arrangements of PDC-109 molecules to form a heparin-binding oligomer...
  36. ncbi request reprint Biochemical and conformational characterisation of HSP-3, a stallion seminal plasma protein of the cysteine-rich secretory protein (CRISP) family
    L Magdaleno
    Instituto de Quimica Fisica Rocasolano, C S I C, Madrid, Spain
    FEBS Lett 420:179-85. 1997
    ..Analysis of the spectroscopic and calorimetric data indicates the occurrence of aggregation of denatured HSP-3 molecules and suggests the monomer as the cooperative unfolding unit...
  37. ncbi request reprint Binding of mannose-6-phosphate and heparin by boar seminal plasma PSP-II, a member of the spermadhesin protein family
    D Solis
    Instituto de Quimica Fisica Rocasolano, C S I C, Madrid, Spain
    FEBS Lett 431:273-8. 1998
    ..In the proposed models, PSP-II Arg43 appears to play a pivotal role in both heparin- and mannose-6-phosphate-complexation as well as in heterodimer formation...
  38. ncbi request reprint Molecular cloning of Echis ocellatus disintegrins reveals non-venom-secreted proteins and a pathway for the evolution of ocellatusin
    Paula Juárez
    Instituto de Biomedicina de Valencia, CSIC, Jaime Roig 11, 46010, Valencia, Spain
    J Mol Evol 63:183-93. 2006
    ....
  39. ncbi request reprint Molecular cloning of disintegrin-like transcript BA-5A from a Bitis arietans venom gland cDNA library: a putative intermediate in the evolution of the long-chain disintegrin bitistatin
    Paula Juárez
    Instituto de Biomedicina de Valencia, CSIC, Jaime Roig 11, 46010 Valencia, Spain
    J Mol Evol 63:142-52. 2006
    ..Whether BA-5A plays a physiological role or represents an orphan protein which could eventually evolve a role in the adaptation of snakes to changing ecological niches and prey habits deserves further investigation...
  40. pmc Molecular cloning of disintegrins from Cerastes vipera and Macrovipera lebetina transmediterranea venom gland cDNA libraries: insight into the evolution of the snake venom integrin-inhibition system
    Libia Sanz
    Instituto de Biomedicina de Valencia, C S I C, Jaume Roig 11, 46010 Valencia, Spain
    Biochem J 395:385-92. 2006
    ....
  41. doi request reprint Snake venomics of the Armenian mountain vipers Macrovipera lebetina obtusa and Vipera raddei
    Libia Sanz
    Instituto de Biomedicina de Valencia, C S I C, Valencia, Spain
    J Proteomics 71:198-209. 2008
    ..l. obtusa and M. l. transmediterranea is less than 4%. Although this result would support the classification of M.l. obtusa and M.l. transmediterranea as different species, additional detailed genomic analyses are also required...
  42. doi request reprint Evolution of snake venom disintegrins by positive Darwinian selection
    Paula Juárez
    Instituto de Biomedicina de Valencia, Consejo Superior de Investigaciones Biológicas, Valencia, Spain
    Mol Biol Evol 25:2391-407. 2008
    ..Perhaps, this represents a consequence of the neofunctionalization potential of the disintegrin domain, a feature that may underlie its recruitment into the venom proteome followed by its successful transformation into a toxin...
  43. pmc Assignment of disulphide bonds in human platelet GPIIIa. A disulphide pattern for the beta-subunits of the integrin family
    J J Calvete
    Instituto de Quimica Fisica, C S I C, Madrid, Spain
    Biochem J 274:63-71. 1991
    ....
  44. ncbi request reprint cDNA cloning and functional expression of jerdostatin, a novel RTS-disintegrin from Trimeresurus jerdonii and a specific antagonist of the alpha1beta1 integrin
    Libia Sanz
    Instituto de Biomedicina de Valencia, C S I C, Jaime Roig 11, 46010 Valencia, Spain
    J Biol Chem 280:40714-22. 2005
    ....
  45. ncbi request reprint The presence of the WGD motif in CC8 heterodimeric disintegrin increases its inhibitory effect on alphaII(b)beta3, alpha(v)beta3, and alpha5beta1 integrins
    Juan J Calvete
    Instituto de Biomedicina de Valencia, CSIC, Valencia 46010, Spain
    Biochemistry 41:2014-21. 2002
    ..We conclude that the WGD motif enhances the inhibitory activity of disintegrins toward alphaII(b)beta3, alpha(v)beta3, and alpha5beta1 integrins...
  46. ncbi request reprint NMR solution structure of the non-RGD disintegrin obtustatin
    M Paz Moreno-Murciano
    Departamento de Qui mica Fi sica, Universitat de Valencia, C Dr Moliner, 50, 46100 Burjassot, Valencia, Spain
    J Mol Biol 329:135-45. 2003
    ..These distinct structural features, along with its different electrostatic surface potential in relation to other known disintegrins, may confer to obtustatin its reported alpha(1)beta(1) integrin inhibitory selectivity...
  47. ncbi request reprint Proteomic identification of actin-derived oligopeptides in dry-cured ham
    Miguel Angel Sentandreu
    Instituto de Agroquimica y Tecnologia de Alimentos, CSIC, P O Box 73, 46100 Burjassot Valencia, Spain
    J Agric Food Chem 55:3613-9. 2007
    ..4.23.5), thus supporting a relevant action of this enzyme during the processing of dry-cured ham...
  48. pmc Conformation and concerted dynamics of the integrin-binding site and the C-terminal region of echistatin revealed by homonuclear NMR
    Daniel Monleon
    Departamento de Quimica Fisica, Universitat de Valencia, Dr Moliner 50, 46100 Burjassot, Valencia, Spain
    Biochem J 387:57-66. 2005
    ..A full-length echistatin-alpha(v)beta3 docking model suggests that echistatin's C-terminal amino acids may contact alpha(v)-subunit residues and provides new insights to delineate structure-function correlations...
  49. ncbi request reprint Insights into the structural basis of the pH-dependent dimer-tetramer equilibrium through crystallographic analysis of recombinant Diocleinae lectins
    Celso S Nagano
    Instituto de Biomedicina de Valencia, C S I C, Jaime Roig 11, 46010 Valencia, Spain
    Biochem J 409:417-28. 2008
    ....
  50. doi request reprint Snake venomics of the Central American rattlesnake Crotalus simus and the South American Crotalus durissus complex points to neurotoxicity as an adaptive paedomorphic trend along Crotalus dispersal in South America
    Juan J Calvete
    Instituto de Biomedicina de Valencia, CSIC, Jaume Roig 11, 46010 Valencia, Spain
    J Proteome Res 9:528-44. 2010
    ..simus envenomations. The identification of evolutionary trends among tropical Crotalus, as reported here, may have an impact in defining the mixture of venoms for immunization to produce an effective pan-American anti-Crotalus antivenom...
  51. ncbi request reprint Snake venomics: characterization of protein families in Sistrurus barbouri venom by cysteine mapping, N-terminal sequencing, and tandem mass spectrometry analysis
    Paula Juárez
    Instituto de Biomedicina de Valencia, C S I C, Valencia, Spain
    Proteomics 4:327-38. 2004
    ..Our results show that the venom proteome of the pigmy rattlesnake S. barbouri is composed of proteins belonging to a few protein families, which can be structurally characterized by their disulfide bond contents...
  52. pmc Amino acid sequence and homology modeling of obtustatin, a novel non-RGD-containing short disintegrin isolated from the venom of Vipera lebetina obtusa
    M Paz Moreno-Murciano
    Instituto de Biomedicina de Valencia, C S I C, Spain
    Protein Sci 12:366-71. 2003
    ..A three-dimensional model of obtustatin, built by homology-modeling structure calculations using different templates and alignments, strongly indicates that the novel KTS motif may reside at the tip of a flexible loop...
  53. ncbi request reprint Sperm coating mechanism from the 1.8 A crystal structure of PDC-109-phosphorylcholine complex
    David A Wah
    Centro de Investigaciones Biologicas, CSIC, Madrid, Spain
    Structure 10:505-14. 2002
    ..The structure of the PDC-109-oPC complex provides a structural ground for the sperm membrane-coating mechanism underlying PDC-109-induced capacitation...
  54. doi request reprint Recombinant expression in human cells of active integrin alpha 1 beta 1-blocking RTS-disintegrin jerdostatin
    Paula Juárez
    Instituto de Biomedicina de Valencia, CSIC, Jaime Roig 11, 46010 Valencia, Spain
    Toxicon 56:1052-8. 2010
    ..Our results highlight pRc-CMV/FLAG-rJerd as a suitable construct for expressing soluble active alpha(1)beta(1)-blocking jerdostatin in a mammalian cell system...
  55. doi request reprint Snake venomics of the South and Central American Bushmasters. Comparison of the toxin composition of Lachesis muta gathered from proteomic versus transcriptomic analysis
    Libia Sanz
    Instituto de Biomedicina de Valencia, C S I C, Valencia, Spain
    J Proteomics 71:46-60. 2008
    ..Our data may contribute to a deeper understanding of the biology and ecology of these snakes, and may also serve as a starting point for studying structure-function correlations of individual toxins...
  56. ncbi request reprint Zinc ions induce the unfolding and self-association of boar spermadhesin PSP-I, a protein with a single CUB domain architecture, and promote its binding to heparin
    Maria A Campanero-Rhodes
    Instituto de Quimica Fisica Rocasolano, CSIC, Serrano 119, 28006 Madrid, Spain
    Biochemistry 45:8227-35. 2006
    ..Thus, the modulation of the structural organization and heparin-binding ability of PSP-I by Zn2+ might be a physiological phenomenon in seminal plasma...
  57. doi request reprint Analysis of O-glycosylation
    Juan J Calvete
    Instituto de Biomedicina de Valencia, CSIC, Valencia, Spain
    Methods Mol Biol 446:281-92. 2008
    ..Nonclustered sites had a sequence context different from that of clustered sites, and charged residues were disfavored at position -1 and +3...
  58. ncbi request reprint Spermadhesin PSP-I/PSP-II heterodimer and its isolated subunits induced neutrophil migration into the peritoneal cavity of rats
    Ana Maria S Assreuy
    Departamento de Ciências Fisiológicas CCS Universidade Estadual do Ceará, Instituto de Biomedicina de Valencia, Spain
    Biol Reprod 67:1796-803. 2002
    ....
  59. ncbi request reprint The first crystal structure of a Mimosoideae lectin reveals a novel quaternary arrangement of a widespread domain
    Francisca Gallego del Sol
    Instituto de Biomedicina de Valencia, CSIC, E 46010 Valencia, Spain
    J Mol Biol 353:574-83. 2005
    ..The cyclic structure of the P.platycephala lectin points to the convergent evolution of a structural principle for the construction of lectins involved in host defense or in attacking other organisms...
  60. ncbi request reprint Crystallization and preliminary X-ray diffraction analysis of the seed lectin from Parkia platycephala
    Francisca Gallego del Sol
    Instituto de Biomedicina de Valencia, CSIC, Jaime Roig 11, E 46010 Valencia, Spain
    Acta Crystallogr D Biol Crystallogr 58:167-9. 2002
    ..Isomorphous heavy-atom derivatives are currently being produced to solve the complete structure of the P. platycephala seed lectin...
  61. ncbi request reprint Conformational diversity of the Goodpasture antigen, the noncollagenous-1 domain of the alpha3 chain of collagen IV
    Juan J Calvete
    Consejo Superior de Investigaciones Cientificas, Instituto de Biomedicina de Valencia, Valencia, Spain
    Proteomics 6:S237-44. 2006
    ..The hitherto unrecognized conformational diversification of the Goodpasture autoantigen may be of relevance in pathogenesis...
  62. pmc Crystallization and preliminary X-ray diffraction analysis of HML, a lectin from the red marine alga Hypnea musciformis
    Celso S Nagano
    Instituto de Biomedicina de Valencia, CSIC, Valencia, Spain
    Acta Crystallogr Sect F Struct Biol Cryst Commun 61:997-9. 2005
    ..A complete data set was collected at 2.4 A resolution. HML is the first marine alga lectin to be crystallized...
  63. ncbi request reprint Analysis of O-glycosylation
    Juan J Calvete
    Instituto de Biomedicina de Valencia, C S I C, Valencia, Spain
    Methods Mol Biol 194:89-100. 2002
  64. doi request reprint Proteomic analysis of phosphorylated nuclear proteins underscores novel roles for rapid actions of retinoic acid in the regulation of mRNA splicing and translation
    Emilio J Laserna
    Biology of Hormone Action Unit, Instituto de Biomedicina de Valencia, Consejo Superior de Investigaciones Cientificas, corrected E 46010 Valencia, Spain
    Mol Endocrinol 23:1799-814. 2009
    ..We conclude that RA activation of signaling pathways can indeed regulate mRNA processing as part of a cellular response orchestrated by the nuclear receptor RAR...
  65. ncbi request reprint Does seminal plasma PSP-I/PSP-II spermadhesin modulate the ability of boar spermatozoa to penetrate homologous oocytes in vitro?
    Ignacio Caballero
    Department of Medicine and Surgery, Faculty of Veterinary Medicine, University of Murcia, Murcia, Spain
    J Androl 25:1004-12. 2004
    ..Such an effect of cryopreservation seems to a certain extent reversible, since cleansing of the sperm surface decreased, at least partially, this blocking effect, increasing both penetration and the monospermic rates...
  66. ncbi request reprint Lebectin, a novel C-type lectin from Macrovipera lebetina venom, inhibits integrin-mediated adhesion, migration and invasion of human tumour cells
    Sameh Sarray
    Laboratoire des Venins et Toxines, Institut Pasteur de Tunis, Tunis, Tunisia
    Lab Invest 84:573-81. 2004
    ..In addition, lebectin proved to be a potent inhibitor of tumour cell proliferation. Although the specific integrins affected by lebectin are not identified in this study, the integrin alpha 5 beta 1 might be involved...
  67. ncbi request reprint Structural requirements of MLD-containing disintegrins for functional interaction with alpha 4 beta 1 and alpha 9 beta1 integrins
    Stanislawa Bazan-Socha
    Department of Biology, College of Science and Technology, Temple University, Philadelphia, Pennsylvania 19122, USA
    Biochemistry 43:1639-47. 2004
    ..These data describe a useful set of potent and selective integrin antagonists and suggest conformational requirements of human and mouse integrins for interaction with ligands...
  68. ncbi request reprint Characterization of a monomeric disintegrin, ocellatusin, present in the venom of the Nigerian carpet viper, Echis ocellatus
    J Bryan Smith
    Department of Pharmacology, Sol Sherry Thrombosis Research Center, Temple University, School of Medicine, Philadelphia, PA 19140, USA
    FEBS Lett 512:111-5. 2002
    ..These mutations may modulate the conformation and/or recognition properties of the integrin-binding loop of ocellatusin...
  69. doi request reprint Snake venomics of the Lesser Antillean pit vipers Bothrops caribbaeus and Bothrops lanceolatus: correlation with toxicological activities and immunoreactivity of a heterologous antivenom
    Jose Maria Gutierrez
    Instituto Clodomiro Picado, Facultad de Microbiologia, Universidad de Costa Rica, San Jose, Costa Rica
    J Proteome Res 7:4396-408. 2008
    ..caribbaeus and B. lanceolatus venoms, and was effective in neutralizing the lethal, hemorrhagic, phospholipase A 2 and proteolytic activities of these venoms...
  70. ncbi request reprint Concerted motions of the integrin-binding loop and the C-terminal tail of the non-RGD disintegrin obtustatin
    Daniel Monleon
    Departamento de Química Física and Servicio Central de Soporte a la Investigación Experimental, Universitat de Valencia, Dr Moliner 50, 46100 Burjassot Valencia, Spain
    J Biol Chem 278:45570-6. 2003
    ..This network of interactions explains the restrained mobility of threonine 22 and suggests that its functional importance lies in maintaining the active conformation of the alpha1beta1 inhibitory loop...
  71. ncbi request reprint Structural determinants of the selectivity of KTS-disintegrins for the alpha1beta1 integrin
    Dariusz G Kisiel
    Center for Neurovirology and Cancer Biology, Temple University, College of Science and Technology, 1900 N, 12th Street, Philadelphia, PA 19122, USA
    FEBS Lett 577:478-82. 2004
    ....
  72. doi request reprint Snake venomics of the lancehead pitviper Bothrops asper: geographic, individual, and ontogenetic variations
    Alberto Alape-Giron
    Instituto Clodomiro Picado, Universidad de Costa Rica, San Jose, Costa Rica
    J Proteome Res 7:3556-71. 2008
    ..asper populations, and highlights the necessity of using pooled venoms as a statistically representative venom for antivenom production...
  73. ncbi request reprint Characterizing the tick carboxypeptidase inhibitor: molecular basis for its two-domain nature
    Joan L Arolas
    Institut de Biotecnologia i Biomedicina and Departament de Bioquimica i Biologia Molecular, Universitat Autonoma de Barcelona, 08193 Bellaterra, Barcelona
    J Biol Chem 281:22906-16. 2006
    ..The results obtained herein provide valuable information for the design of more potent and selective TCI molecules...
  74. ncbi request reprint Crystal structure of a prostate kallikrein isolated from stallion seminal plasma: a homologue of human PSA
    Ana L Carvalho
    REQUIMTE CQFB, Departamento de Quimica, Fac de Ciencias e Tecnologia, Universidade Nova de Lisboa, 2829 516 Caparica, Portugal
    J Mol Biol 322:325-37. 2002
    ..Crystal soaking experiments revealed a binding site for Zn(2+) and Hg(2+), two known PSA inhibitors...
  75. ncbi request reprint Production and characterisation of recombinant forms of human pulmonary surfactant protein C (SP-C): Structure and surface activity
    Dunja Lukovic
    Departament de Bioquimica i Biologia Molecular, Universitat de Valencia, E 46 100 Burjassot Valencia, Spain
    Biochim Biophys Acta 1758:509-18. 2006
    ....
  76. ncbi request reprint Analysis of porcine peripheral blood mononuclear cells proteome by 2-DE and MS: analytical and biological variability in the protein expression level and protein identification
    María Ramírez-Boo
    Unidad Mixta C S I C UCO Marcadores Genéticos Moleculares en Animales Domésticos, Departamento de Genetica, Universidad de Cordoba, Campus Rabanales, Cordoba, Spain
    Proteomics 6:S215-25. 2006
    ..Finally, other proteins implicated in the cell signaling process, transport or apoptosis were also identified giving a wide overview of the porcine PBMC protein map...
  77. ncbi request reprint Isolation of two novel mannan- and L-fucose-binding lectins from the green alga Enteromorpha prolifera: biochemical characterization of EPL-2
    Andrea L Ambrosio
    Instituto de Química y Fisicoquímica Biológicas UBA CONICET, Facultad de Farmacia y Bioquimica, Junin 956, 1113 Buenos Aires, Argentina
    Arch Biochem Biophys 415:245-50. 2003
    ....
  78. ncbi request reprint Loss of introns along the evolutionary diversification pathway of snake venom disintegrins evidenced by sequence analysis of genomic DNA from Macrovipera lebetina transmediterranea and Echis ocellatus
    Amine Bazaa
    Laboratoire des Venins et Toxines, Institut Pasteur de Tunis, B P 74, 1002, Tunis Belvedere, Tunisia
    J Mol Evol 64:261-71. 2007
    ..However, a comparative analysis of currently available disintegrin(-like) genes outlines the view that a minimization of both the gene organization and the protein structure underlies the evolution of the snake venom disintegrin family...
  79. ncbi request reprint Snake venomics: comparative analysis of the venom proteomes of the Tunisian snakes Cerastes cerastes, Cerastes vipera and Macrovipera lebetina
    Amine Bazaa
    Laboratoire des Venins et Toxines, Institut Pasteur de Tunis, Tunis Belvedere, Tunisia
    Proteomics 5:4223-35. 2005
    ....
  80. ncbi request reprint Role of an intrasubunit disulfide in the association state of the cytosolic homo-oligomer methionine adenosyltransferase
    Gabino F Sanchez-Perez
    Instituto de Investigaciones Biomedicas Alberto Sols, Consejo Superior de Investigaciones Científicas CSIC Universidad Autónoma de Madrid, Arturo Duperier 4, 28029 Madrid, Spain
    J Biol Chem 278:7285-93. 2003
    ..In addition, a kinetic intermediate in the MAT I dissociation to MAT III has been detected. The physiological importance of these results is discussed in light of the structural and regulatory data available...
  81. ncbi request reprint Snake venomics of Central American pitvipers: clues for rationalizing the distinct envenomation profiles of Atropoides nummifer and Atropoides picadoi
    Yamileth Angulo
    Instituto Clodomiro Picado, Facultad de Microbiologia, Universidad de Costa Rica, San Jose, Costa Rica
    J Proteome Res 7:708-19. 2008
    ..nummifer snakebite in comparison to other crotaline snake venoms and the high hemorrhagic activity of A. picadoi...
  82. doi request reprint A new type of metal-binding site in cobalt- and zinc-containing adenylate kinases isolated from sulfate-reducers Desulfovibrio gigas and Desulfovibrio desulfuricans ATCC 27774
    Olga Yu Gavel
    REQUIMTE, Departamento de Quimica, Centro de Quimica Fina e Biotecnologia, Faculdade de Ciencias e Tecnologia, Universidade Nova de Lisboa, 2829 516 Caparica, Portugal
    J Inorg Biochem 102:1380-95. 2008
    ..gigas is involved in metal coordination and represents a new type of binding motif that differs from other known zinc-binding sites of AK. Cobalt and zinc play a structural role in stabilizing the LID domain...
  83. doi request reprint Point mutations abolishing the mannose-binding capability of boar spermadhesin AQN-1
    Mahnaz Ekhlasi-Hundrieser
    Department of Reproductive Biology, University of Veterinary Medicine Hannover, Foundation, Bünteweg 2, 30559 Hannover, Germany
    Biochim Biophys Acta 1784:856-62. 2008
    ..This indicates that different spermadhesin molecules have evolved non-equivalent carbohydrate-binding capabilities, which may underlie their distinct patterns of biological activities...
  84. doi request reprint Snake venomics and antivenomics of the arboreal neotropical pitvipers Bothriechis lateralis and Bothriechis schlegelii
    Bruno Lomonte
    Instituto Clodomiro Picado, Facultad de Microbiologia, Universidad de Costa Rica, San Jose, Costa Rica
    J Proteome Res 7:2445-57. 2008
    ....
  85. ncbi request reprint Obtustatin: a potent selective inhibitor of alpha1beta1 integrin in vitro and angiogenesis in vivo
    Cezary Marcinkiewicz
    Temple University, School of Medicine, Thrombosis Research Center, Philadelphia, Pennsylvania 19140, USA
    Cancer Res 63:2020-3. 2003
    ....
  86. ncbi request reprint Influence of seminal plasma PSP-I/PSP-II spermadhesin on pig gamete interaction
    Ignacio Caballero
    Department of Medicine and Surgery, Faculty of Veterinary Medicine, University of Murcia, Murcia, Spain
    Zygote 13:11-6. 2005
    ..In addition, when PSP-I/PSP-II was added to the IVM medium, oocyte maturation rates were significantly reduced. In conclusion, the results suggest that PSP-I/PSP-II, when present in vitro, blocks sperm-ZP binding...
  87. ncbi request reprint Dissecting the protective effect of the seminal plasma spermadhesin PSP-I/PSP-II on boar sperm functionality
    Eva M Garcia
    Departamento de Medicina y Cirugia Animal, Facultad de Veterinaria, Universidad de Murcia, Campus de Espinardo, 30071 Murcia, Spain
    J Androl 27:434-43. 2006
    ....
  88. ncbi request reprint Immunolocalization and possible functional role of PSP-I/PSP-II heterodimer in highly extended boar spermatozoa
    Ignacio Caballero
    Departamento de Medicina y Cirugia Animal, Facultad de Veterinaria, Universidad de Murcia, Murcia, Spain
    J Androl 27:766-73. 2006
    ..In conclusion, the protective effect of the heterodimer appears to be related to its adhesion to the acrosomal area, and the loss of this protective effect coincides with a stepwise redistribution of PSP-I/PSP-II during incubation...
  89. ncbi request reprint Seed lectin from pisum arvense: isolation, biochemical characterization and amino acid sequence
    Benildo S Cavada
    Departamento de Bioquimica e Biologia Molecular, Campus do Pici, Caixa Postal 6033, Fortaleza Ceará CEP 60451 970, Brazil
    Protein Pept Lett 10:607-17. 2003
    ..It is discussed that P. arvense should be considered as a synonym of P. sativum. Furthermore, like pea lectin, PAL discriminates biantennary fucosylated glycan, determined by surface plasmon resonance...
  90. ncbi request reprint Boar spermatozoa in the oviduct
    Heriberto Rodriguez-Martinez
    Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine and Animal Science, Swedish University of Agricultural Sciences SLU, Ullsvägen 14C, Clinical Centre, Ultuna, Uppsala, Sweden
    Theriogenology 63:514-35. 2005
    ....
  91. ncbi request reprint Influence of porcine spermadhesins on the susceptibility of boar spermatozoa to high dilution
    Fernando Centurion
    Departamento de Medicina y Cirugia Animal, Facultad de Veterinaria, Universidad de Murcia, Murcia, Spain
    Biol Reprod 69:640-6. 2003
    ....
  92. ncbi request reprint Isolation and characterization of the main small heat shock proteins induced in tomato pericarp by thermal treatment
    Gustavo A Polenta
    Instituto de Tecnologia de Alimentos, Instituto Nacional de Tecnologia Agropecuaria, Castelar, Argentina
    FEBS J 274:6447-55. 2007
    ..Our data are valuable for the future development of analytical methods to evaluate the optimal protection induced by heat treatment in different fruits...
  93. ncbi request reprint Crystallization and preliminary X-ray diffraction analysis of the lectin from Canavalia gladiata seeds
    Frederico B M B Moreno
    Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Ceara, Fortaleza, CE 60451 970, Brazil
    Acta Crystallogr D Biol Crystallogr 60:1493-5. 2004
    ..3 A resolution. The preliminary crystal structure of the lectin, determined by molecular replacement, had a correlation coefficient of 0.569 and an R factor of 0.412...