Joaquin Arino

..This review will summarize the latest advances in our understanding about how these phosphatases are regulated and fulfil their functions in the yeast cell...

..In this review we will examine these responses, their possible interactions, and the role that they play in tolerance to high pH stress...

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..In this work we review the available literature on fungal PP2Cs and explore sequence databases to provide a comprehensive overview of these enzymes in fungi...

..These results point to a complex role of Ypi1 on the regulation of cation homeostasis, largely mediated by the calcineurin phosphatase...

..In contrast, the mutation of His378 did not. This finding suggests that Hal3 may have both Ppz1-dependent and independent functions involving different structural elements...

..Remarkably, the evidence collected indicates that the role of Ref2 in cation homoeostasis may be unrelated to its previously identified function in the formation of mRNA via the APT (for associated with Pta1) complex...

..These results indicate that, besides its role as Ppz1 inhibitory subunit, Vhs3 (and probably Hal3) might have important Ppz-independent functions...

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..We postulate that lack of Pif1 forces aconitase to play its DNA protective role as a nucleoid protein and that this triggers a domino effect on iron homoeostasis resulting in increased zinc tolerance...

..Our results unveil Hal3 and Vhs3 as moonlighting proteins involved in both CoA biosynthesis and protein phosphatase regulation...

..This suggests that the control exerted by calcineurin on Hal5 expression may be biologically relevant for Trk1 regulation...

..These results indicate that Ptc1p modulates the function of Ena1p by regulating the Hal3/Ppz1,2 pathway. In conclusion, overexpression of PTC3 and lack of PTC1 affect lithium tolerance in yeast, although through different mechanisms...

..All of these results provide the first evidence showing that a type 2C protein phosphatase is required for the normal functioning of the TOR pathway...

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..Thus, the transcriptional response to alkaline stress of the ENA1 gene integrates three different signaling pathways...

..Deletion of SWE1 restored normal Cdc11 levels in the absence of Ypi1. These results demonstrate that Ypi1 plays an important role in the morphogenesis checkpoint, possibly by regulating Swe1...

..At any rate, this study paves the way for further application of gene signatures to more stringent biopsy discrimination challenges...

..These findings suggest that high zinc levels may alter the assembly and/or function of iron-sulphur-containing proteins, as well as the biosynthesis of haem groups, thus establishing a link between zinc, iron and sulphur metabolism...

..This is particularly useful given the large variety of important biological processes performed by these of enzymes, often necessaries for cell survival, which makes in some cases infeasible the generation of null mutants...

..A subset of 27 representative mutants were selected and subjected to diverse biochemical tests that, in some cases, allowed us to postulate the basis for the observed phenotypes...

..In conclusion, we have identified a novel role for calcineurin, but not alkalinization, in the control of ENA1 expression in ppz1 mutants...

..This model highlights an unsuspected link between potassium homeostasis and these important morphogenetic events...

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..We propose that alkalinization of the medium results in impaired glucose utilization and that activation of certain glucose-metabolizing genes by calcineurin contributes to yeast survival under this stress situation...

..However, the relevance of attenuation of PKA in high pH tolerance is probably not restricted to regulation of Msn2 function...

..The later is rather conserved in other related antiporters, while the former is not...

..Herewith, we demonstrate that primary brain tumor types can be discriminated using microarray data in an automated and objective way...

..Therefore, its use in the past should be revisited and utilization of this marker should be avoided in future analyses...

..Our results are consistent with a model in which CK2 plays a key role in cell division and cell expansion, with compelling effects on Arabidopsis development...

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..The possible relationship between the known functions of these suppressor genes and the progress through the G(1)-S transition is discussed...

..Genes non-previously related to prion diseases should be taken into consideration for further investigations...

..Thus, even in a scenario of low coverage the concordance in the measurements is quite acceptable. On the other hand, the comparatively longer read size obtained by pyrosequencing allows detecting unconventional splicing forms...

..These results demonstrate that the transcriptional response to alkaline pH involves different signalling mechanisms, and that calcium signalling is a relevant component of this response...