J F Aparicio

Summary

Country: Spain

Publications

  1. ncbi request reprint A complex multienzyme system encoded by five polyketide synthase genes is involved in the biosynthesis of the 26-membered polyene macrolide pimaricin in Streptomyces natalensis
    J F Aparicio
    Institute of Biotechnology INBIOTEC, Parque Cientifico de Leon, Leon, France
    Chem Biol 7:895-905. 2000
  2. ncbi request reprint Polyene antibiotic biosynthesis gene clusters
    J F Aparicio
    INBIOTEC, Institute of Biotechnology of León, Av Real 1, 24006, Leon, Spain
    Appl Microbiol Biotechnol 61:179-88. 2003
  3. ncbi request reprint Polyene macrolide antibiotic biosynthesis
    J F Aparicio
    Institute of Biotechnology INBIOTEC, Parque Cientifico de Leon, and Area of Microbiology, Faculty of Biology, University of Leon, Leon, Spain
    Curr Med Chem 11:1645-56. 2004
  4. ncbi request reprint Engineered biosynthesis of novel polyenes: a pimaricin derivative produced by targeted gene disruption in Streptomyces natalensis
    M V Mendes
    Institute of Biotechnology INBIOTEC, , Spain
    Chem Biol 8:635-44. 2001
  5. ncbi request reprint Characterization of the ask-asd operon in aminoethoxyvinylglycine-producing Streptomyces sp. NRRL 5331
    Y Cuadrado
    , , Avda del Real no 1, 24006, , Spain
    Appl Microbiol Biotechnol 64:228-36. 2004
  6. ncbi request reprint Nitrate regulation of alpha-aminoadipate reductase formation and lysine inhibition of its activity in Penicillium chrysogenum and Acremonium chrysogenum
    M J Hijarrubia
    Instituto de Biotecnologia de Leon, INBIOTEC, Parque Cientifico de Leon, Avda del Real, No 1, Spain
    Appl Microbiol Biotechnol 59:270-7. 2002
  7. ncbi request reprint Characterization of the lys2 gene of Acremonium chrysogenum encoding a functional alpha-aminoadipate activating and reducing enzyme
    M J Hijarrubia
    Institute of Biotechnology INBIOTEC, Science Park of León, Spain
    Mol Gen Genet 264:755-62. 2001
  8. ncbi request reprint Organisation of the biosynthetic gene cluster for rapamycin in Streptomyces hygroscopicus: analysis of genes flanking the polyketide synthase
    I Molnar
    Cambridge Centre for Molecular Recognition, Department of Biochemistry, University of Cambridge, UK
    Gene 169:1-7. 1996

Collaborators

  • J F Martin
  • M J Hijarrubia
  • Y Cuadrado
  • E Recio
  • M V Mendes
  • I Molnar
  • R Luiten
  • R Fouces
  • J Casqueiro
  • T Schwecke
  • L E Khaw
  • A Konig
  • P F Leadlay
  • J Staunton
  • S F Haydock

Detail Information

Publications8

  1. ncbi request reprint A complex multienzyme system encoded by five polyketide synthase genes is involved in the biosynthesis of the 26-membered polyene macrolide pimaricin in Streptomyces natalensis
    J F Aparicio
    Institute of Biotechnology INBIOTEC, Parque Cientifico de Leon, Leon, France
    Chem Biol 7:895-905. 2000
    ..We set out to clone, sequence and analyse the gene cluster responsible for the biosynthesis of this tetraene...
  2. ncbi request reprint Polyene antibiotic biosynthesis gene clusters
    J F Aparicio
    INBIOTEC, Institute of Biotechnology of León, Av Real 1, 24006, Leon, Spain
    Appl Microbiol Biotechnol 61:179-88. 2003
    ..Successful genetic manipulation of the producing organisms leading to production of polyene analogues indicates good prospects for generating improved antifungal compounds via genetic engineering...
  3. ncbi request reprint Polyene macrolide antibiotic biosynthesis
    J F Aparicio
    Institute of Biotechnology INBIOTEC, Parque Cientifico de Leon, and Area of Microbiology, Faculty of Biology, University of Leon, Leon, Spain
    Curr Med Chem 11:1645-56. 2004
    ....
  4. ncbi request reprint Engineered biosynthesis of novel polyenes: a pimaricin derivative produced by targeted gene disruption in Streptomyces natalensis
    M V Mendes
    Institute of Biotechnology INBIOTEC, , Spain
    Chem Biol 8:635-44. 2001
    ..This novel epoxidase could prove to be valuable for the introduction of epoxy substituents into designer macrolides...
  5. ncbi request reprint Characterization of the ask-asd operon in aminoethoxyvinylglycine-producing Streptomyces sp. NRRL 5331
    Y Cuadrado
    , , Avda del Real no 1, 24006, , Spain
    Appl Microbiol Biotechnol 64:228-36. 2004
    ..This is the first report in which a gene cluster for the first two steps of aminoethoxyvinylglycine biosynthesis is characterized...
  6. ncbi request reprint Nitrate regulation of alpha-aminoadipate reductase formation and lysine inhibition of its activity in Penicillium chrysogenum and Acremonium chrysogenum
    M J Hijarrubia
    Instituto de Biotecnologia de Leon, INBIOTEC, Parque Cientifico de Leon, Avda del Real, No 1, Spain
    Appl Microbiol Biotechnol 59:270-7. 2002
    ..Nitrate has a strong negative effect on alpha-AAR formation as shown by immunoblotting studies of alpha-AAR. The nitrate effect was reversed by lysine...
  7. ncbi request reprint Characterization of the lys2 gene of Acremonium chrysogenum encoding a functional alpha-aminoadipate activating and reducing enzyme
    M J Hijarrubia
    Institute of Biotechnology INBIOTEC, Science Park of León, Spain
    Mol Gen Genet 264:755-62. 2001
    ..chrysogenum C10 (the chromosome that contains the "late" cephalosporin cluster) and is transcribed as a monocistronic 4.5-kb mRNA although at relatively low levels compared with the beta-actin gene...
  8. ncbi request reprint Organisation of the biosynthetic gene cluster for rapamycin in Streptomyces hygroscopicus: analysis of genes flanking the polyketide synthase
    I Molnar
    Cambridge Centre for Molecular Recognition, Department of Biochemistry, University of Cambridge, UK
    Gene 169:1-7. 1996
    ..Adjacent genes have putative roles in Rp regulation and export. The codon usage of the PKS biosynthetic genes is markedly different from that of the flanking genes of the cluster...