Balbino Alarcon

Summary

Country: Spain

Publications

  1. pmc MTOC translocation modulates IS formation and controls sustained T cell signaling
    Noa B Martín-Cófreces
    Servicio de Inmunologia, Hospital de la Princesa, Universidad Autonoma de Madrid, 28006 Madrid, Spain
    J Cell Biol 182:951-62. 2008
  2. doi request reprint H-ras and N-ras are dispensable for T-cell development and activation but critical for protective Th1 immunity
    Salvador Iborra
    Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientificas, Departamento de Biologia Molecular, Universidad Autonoma de Madrid, Cantoblanco, Madrid, Spain
    Blood 117:5102-11. 2011
  3. pmc RRas2, RhoG and T-cell phagocytosis
    Balbino Alarcon
    Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientificas, Universidad Autonoma de Madrid, Madrid, Spain
    Small Gtpases 3:97-101. 2012
  4. doi request reprint Two receptors, two kinases, and T cell lineage determination
    Balbino Alarcon
    Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientificas, Universidad Autonoma de Madrid, Cantoblanco, Madrid 28049, Spain
    Sci Signal 3:pe11. 2010
  5. ncbi request reprint Initiation of TCR signaling: regulation within CD3 dimers
    Balbino Alarcon
    Centro de Biologia Molecular Severo Ochoa, CSIC Universidad Autonoma de Madrid, Madrid, Spain
    Immunol Rev 191:38-46. 2003
  6. pmc The immunological synapse: a cause or consequence of T-cell receptor triggering?
    Balbino Alarcon
    Centro de Biologia Molecular Severo Ochoa, CSIC UAM, Madrid, Spain
    Immunology 133:420-5. 2011
  7. ncbi request reprint Ligand-induced conformational change in the T-cell receptor associated with productive immune synapses
    Ruth M Risueno
    Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientificas, Universidad Autonoma de Madrid, Cantoblanco Madrid 28049, Spain
    Blood 106:601-8. 2005
  8. pmc A conformational change senses the strength of T cell receptor-ligand interaction during thymic selection
    Ruth M Risueno
    Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientificas, Universidad Autonoma de Madrid, 28049 Madrid, Spain
    Proc Natl Acad Sci U S A 103:9625-30. 2006
  9. doi request reprint T cell receptor internalization from the immunological synapse is mediated by TC21 and RhoG GTPase-dependent phagocytosis
    Nuria Martínez-Martín
    Centro de Biologia Molecular Severo Ochoa, CSIC UAM, 28049 Madrid, Spain
    Immunity 35:208-22. 2011
  10. pmc T cell receptor engagement triggers its CD3epsilon and CD3zeta subunits to adopt a compact, locked conformation
    Ruth M Risueno
    Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientificas, Universidad Autonoma de Madrid, Madrid, Spain
    PLoS ONE 3:e1747. 2008

Collaborators

Detail Information

Publications25

  1. pmc MTOC translocation modulates IS formation and controls sustained T cell signaling
    Noa B Martín-Cófreces
    Servicio de Inmunologia, Hospital de la Princesa, Universidad Autonoma de Madrid, 28006 Madrid, Spain
    J Cell Biol 182:951-62. 2008
    ..Together, these data reveal MTOC translocation as an important mechanism underlying IS formation and sustained T cell signaling...
  2. doi request reprint H-ras and N-ras are dispensable for T-cell development and activation but critical for protective Th1 immunity
    Salvador Iborra
    Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientificas, Departamento de Biologia Molecular, Universidad Autonoma de Madrid, Cantoblanco, Madrid, Spain
    Blood 117:5102-11. 2011
    ..Furthermore, we demonstrate for the first time that H-ras and N-ras act as critical controllers of Th1 responses, mostly by transmitting TCR signals for Th1 priming of CD4⁺ T cells...
  3. pmc RRas2, RhoG and T-cell phagocytosis
    Balbino Alarcon
    Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientificas, Universidad Autonoma de Madrid, Madrid, Spain
    Small Gtpases 3:97-101. 2012
    ..In this view, we discuss the participation of TC21 in the specific process of T cell antigen receptor internalization from the immunological synapse and acquisition of membrane fragments from the antigen presenting cells by phagocytosis...
  4. doi request reprint Two receptors, two kinases, and T cell lineage determination
    Balbino Alarcon
    Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientificas, Universidad Autonoma de Madrid, Cantoblanco, Madrid 28049, Spain
    Sci Signal 3:pe11. 2010
    ..This finding, together with the known importance of Lck in the determination of CD4(+) and CD8(+) lineages, enables us to propose that a balance between the activation of these two kinases by the TCR determines lineage decisions...
  5. ncbi request reprint Initiation of TCR signaling: regulation within CD3 dimers
    Balbino Alarcon
    Centro de Biologia Molecular Severo Ochoa, CSIC Universidad Autonoma de Madrid, Madrid, Spain
    Immunol Rev 191:38-46. 2003
    ..How the presence of TCR in clusters influences TCR avidity and propagation of TCR signals is something that has yet to be investigated...
  6. pmc The immunological synapse: a cause or consequence of T-cell receptor triggering?
    Balbino Alarcon
    Centro de Biologia Molecular Severo Ochoa, CSIC UAM, Madrid, Spain
    Immunology 133:420-5. 2011
    ..Here, we shall review the historical evolution of the role of the cSMAC in T-cell activation, finally discussing our most recent data indicating that the cSMAC serves to internalize exhausted TCRs by phagocytosis...
  7. ncbi request reprint Ligand-induced conformational change in the T-cell receptor associated with productive immune synapses
    Ruth M Risueno
    Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientificas, Universidad Autonoma de Madrid, Cantoblanco Madrid 28049, Spain
    Blood 106:601-8. 2005
    ..Therefore, these results demonstrate that the TCR undergoes a conformational change on MHCp binding in vitro and in vivo, and they establish a molecular correlate for productive TCR engagement...
  8. pmc A conformational change senses the strength of T cell receptor-ligand interaction during thymic selection
    Ruth M Risueno
    Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientificas, Universidad Autonoma de Madrid, 28049 Madrid, Spain
    Proc Natl Acad Sci U S A 103:9625-30. 2006
    ..Thus, the conformational change in the TCR may underlie the discrimination of ligands leading to positive and negative selection...
  9. doi request reprint T cell receptor internalization from the immunological synapse is mediated by TC21 and RhoG GTPase-dependent phagocytosis
    Nuria Martínez-Martín
    Centro de Biologia Molecular Severo Ochoa, CSIC UAM, 28049 Madrid, Spain
    Immunity 35:208-22. 2011
    ..Therefore, TC21 and RhoG dependence underlie the existence of a common phagocytic mechanism that drives TCR internalization from the IS together with its peptide-MHC ligand...
  10. pmc T cell receptor engagement triggers its CD3epsilon and CD3zeta subunits to adopt a compact, locked conformation
    Ruth M Risueno
    Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientificas, Universidad Autonoma de Madrid, Madrid, Spain
    PLoS ONE 3:e1747. 2008
    ..These results are consistent with a model in which transduction of the conformational change induced upon TCR triggering promotes condensation and shielding of the CD3 cytoplasmic tails...
  11. pmc End-binding protein 1 controls signal propagation from the T cell receptor
    Noa B Martín-Cófreces
    Servicio de Inmunologia, Hospital Universitario de la Princesa, Universidad Autonoma de Madrid, Instituto Investigación Sanitaria Princesa IIS IP, Madrid, Spain
    EMBO J 31:4140-52. 2012
    ..These results identify a role for EB1 interaction with the TCR in controlling TCR sorting and its connection with the LAT/PLCγ1 signalosome...
  12. doi request reprint Relevance of Nck-CD3{varepsilon} Interaction for T Cell Activation In Vivo
    Aldo Borroto
    Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientificas, Universidad Autonoma de Madrid, Cantoblanco, Madrid 28049, Spain
    J Immunol 192:2042-53. 2014
    ..These data suggest that Nck recruitment to the TCR is fundamental to mount an efficient T cell response in vivo, and that the Nck-CD3ε interaction may represent a target for pharmacological modulation of the immune response. ..
  13. pmc Nck recruitment to the TCR required for ZAP70 activation during thymic development
    Aldo Borroto
    Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientificas, Universidad Autonoma de Madrid, Cantoblanco, Madrid 28049, Spain
    J Immunol 190:1103-12. 2013
    ..Differences in the extent of PRS mutation could explain the phenotypic differences in both knockin mice...
  14. ncbi request reprint Engaged and bystander T cell receptors are down-modulated by different endocytotic pathways
    Alicia Monjas
    Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientificas, Universidad Autonoma de Madrid, Cantoblanco, Madrid 28049, Spain
    J Biol Chem 279:55376-84. 2004
    ..Finally, we propose that although both mechanisms coexist, the predominance of one or the other mechanisms will depend on the dose of ligand...
  15. doi request reprint Increased sensitivity of antigen-experienced T cells through the enrichment of oligomeric T cell receptor complexes
    Rashmi Kumar
    Departamento de Biología Celular e Inmunología, Centro Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientificas, Universidad Autonoma de Madrid, 28049 Madrid, Spain
    Immunity 35:375-87. 2011
    ..Thus, we propose that an "avidity maturation" mechanism underlies T cell antigenic memory...
  16. pmc Activation outcomes induced in naïve CD8 T-cells by macrophages primed via "phagocytic" and nonphagocytic pathways
    Isabel María Olazabal
    Servicio de Inmunologia, Hospital de la Princesa, Universidad Autonoma de Madrid, 28006 Madrid, Spain
    Mol Biol Cell 19:701-10. 2008
    ..These results suggest that the pathway used for antigen uptake may modulate the antigen density presented on MHC-I, resulting in different signals induced in naïve CD8 T-cells, leading either to CD8 T-cell activation or anergy...
  17. doi request reprint Cooperativity between T cell receptor complexes revealed by conformational mutants of CD3epsilon
    Nuria Martínez-Martín
    Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientificas, Universidad Autonoma de Madrid, Cantoblanco, Madrid 28049, Spain
    Sci Signal 2:ra43. 2009
    ..These results emphasize the importance of the conformational change in CD3epsilon for the activation of T cells and suggest the existence of unforeseen cooperativity between TCR complexes...
  18. doi request reprint Conformational model
    Ruth M Risueno
    Centro de Biologia Molecular Severo Ochoa, CSIC Universidad Autonoma de Madrid, Cantoblanco, Madrid 28049, Spain
    Adv Exp Med Biol 640:103-12. 2008
    ....
  19. pmc Coexistence of multivalent and monovalent TCRs explains high sensitivity and wide range of response
    Wolfgang W A Schamel
    Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientificas, Universidad Autonoma de Madrid, Madrid 28049, Spain
    J Exp Med 202:493-503. 2005
    ..Thus, besides resolving TCR stoichiometry, these data can explain how T cells respond to a wide range of MHCp concentrations while maintaining high sensitivity...
  20. doi request reprint Expression of a modified form of CD4 results in the release of an anti-HIV factor derived from the Env sequence
    Irene Zaldivar
    Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientificas Universidad Autonoma de Madrid, Spain
    J Immunol 183:1188-96. 2009
    ..The presence of EDAF improves the therapeutic potential of the CD4epsilon15 gene and it opens new possibilities for antiviral treatment and vaccine development...
  21. doi request reprint Essential function for the GTPase TC21 in homeostatic antigen receptor signaling
    Pilar Delgado
    Centro de Biologia Molecular Severo Ochoa, Universidad Autonoma de Madrid, Cantoblanco, Spain
    Nat Immunol 10:880-8. 2009
    ..Consequently, we propose TC21 directly links antigen receptors to PI(3)K-mediated survival pathways...
  22. ncbi request reprint Role of Fyn in the rearrangement of tubulin cytoskeleton induced through TCR
    Noa B Martín-Cófreces
    Servicio de Inmunologia, Hospital de la Princesa, Universidad Autonoma de Madrid, Spain
    J Immunol 176:4201-7. 2006
    ..In addition, Fyn deficiency significantly affects the MTOC relocation mediated by agonist peptide stimulation. These results reveal Fyn to be a key regulator of tubulin cytoskeleton reorganization in T cells...
  23. pmc An orderly inactivation of intracellular retention signals controls surface expression of the T cell antigen receptor
    Pilar Delgado
    Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientificas, Universidad Autonoma de Madrid, Madrid 28049, Spain
    J Exp Med 201:555-66. 2005
    ..We propose that the stepwise assembly of the TCR complex guarantees that all assembly intermediates have at least one functional ER retention signal and that only a full signaling-competent TCR complex is expressed on the cell surface...
  24. ncbi request reprint Overlapping functions of human CD3delta and mouse CD3gamma in alphabeta T-cell development revealed in a humanized CD3gamma-mouse
    Edgar Fernández-Malavé
    Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientificas, Universidad Autonoma de Madrid, Cantoblanco, 28049 Madrid, Spain
    Blood 108:3420-7. 2006
    ..Taken together, these data demonstrate that CD3delta and CD3gamma play a different role in humans and mice in pre-TCR and TCR function during alphabeta T-cell development...
  25. ncbi request reprint Recruitment of Nck by CD3 epsilon reveals a ligand-induced conformational change essential for T cell receptor signaling and synapse formation
    Diana Gil
    Centro de Biologia Molecular Severo Ochoa, Consejo Superior de Investigaciones Cientificas, Universidad Autonoma de Madrid, Cantoblanco, Madrid 20849, Spain
    Cell 109:901-12. 2002
    ..Finally, by interfering with Nck-CD3 epsilon association in vivo, we demonstrate that TCR-CD3 recruitment of Nck is critical for maturation of the immune synapse and for T cell activation...