Wendy S Stevens

Summary

Affiliation: University of the Witwatersrand
Country: South Africa

Publications

  1. pmc Metabolic and anthropometric parameters contribute to ART-mediated CD4+ T cell recovery in HIV-1-infected individuals: an observational study
    Livio Azzoni
    HIV 1 Immunopathogenesis Laboratory, The Wistar Institute, Philadelphia, PA, USA
    J Int AIDS Soc 14:37. 2011
  2. pmc The net cost of incorporating resistance testing into HIV/AIDS treatment in South Africa: a Markov model with primary data
    Sydney Rosen
    Center for Global Health and Development, Boston University, Boston, MA, USA
    J Int AIDS Soc 14:24. 2011
  3. pmc Association between HIV replication and serum leptin levels: an observational study of a cohort of HIV-1-infected South African women
    Livio Azzoni
    The Wistar Institute, Philadelphia, PA, USA
    J Int AIDS Soc 13:33. 2010
  4. pmc Utility of clinical parameters to identify HIV infection in infants below ten weeks of age in South Africa: a prospective cohort study
    Heather B Jaspan
    Children s Infectious Diseases Clinical Research Unit, Department of Paediatrics and Child Health and Centre for Infectious Diseases, Stellenbosch University, Tygerberg, South Africa
    BMC Pediatr 11:104. 2011
  5. pmc A comparative analysis of HIV drug resistance interpretation based on short reverse transcriptase sequences versus full sequences
    Kim Steegen
    Department of Infectious Disease and Biomarkers, Tibotec Virco Virology BVBA, Beerse, Belgium
    AIDS Res Ther 7:38. 2010
  6. pmc Polychromatic immunophenotypic characterization of T cell profiles among HIV-infected patients experiencing immune reconstitution inflammatory syndrome (IRIS)
    David M Murdoch
    Division of Pulmonary and Critical Care Medicine, Duke University Medical Center, Durham, North Carolina, USA
    AIDS Res Ther 6:16. 2009
  7. pmc Multi-analyte profiling of ten cytokines in South African HIV-infected patients with Immune Reconstitution Inflammatory Syndrome (IRIS)
    Catherine M Worsley
    Department of Molecular Medicine and Haematology, Faculty of Health Sciences, University of the Witwatersrand and National Health Laboratory Services, Johannesburg, South Africa
    AIDS Res Ther 7:36. 2010
  8. pmc Ultra-high-throughput, automated nucleic acid detection of human immunodeficiency virus (HIV) for infant infection diagnosis using the Gen-Probe Aptima HIV-1 screening assay
    Wendy S Stevens
    Department of Molecular Medicine and Haematology, University of the Witwatersrand, Parktown, Johannesburg, South Africa
    J Clin Microbiol 47:2465-9. 2009
  9. doi request reprint Challenges in implementing HIV load testing in South Africa
    Wendy S Stevens
    Department of Molecular Medicine and Haematology, National Health Laboratory Service and University of the Witwatersrand, Johannesburg, South Africa
    J Infect Dis 201:S78-84. 2010
  10. doi request reprint Quantifying HIV for monitoring antiretroviral therapy in resource-poor settings
    Wendy S Stevens
    Department of Molecular Medicine and Haematology, University of Witwatersrand, Johannesburg, South Africa
    J Infect Dis 201:S16-26. 2010

Collaborators

Detail Information

Publications39

  1. pmc Metabolic and anthropometric parameters contribute to ART-mediated CD4+ T cell recovery in HIV-1-infected individuals: an observational study
    Livio Azzoni
    HIV 1 Immunopathogenesis Laboratory, The Wistar Institute, Philadelphia, PA, USA
    J Int AIDS Soc 14:37. 2011
    ..We sought to determine if adiposity and molecules associated with lipid metabolism may affect the response to ART and the degree of subsequent immune reconstitution, and to assess their ability to predict CD4 recovery...
  2. pmc The net cost of incorporating resistance testing into HIV/AIDS treatment in South Africa: a Markov model with primary data
    Sydney Rosen
    Center for Global Health and Development, Boston University, Boston, MA, USA
    J Int AIDS Soc 14:24. 2011
    ..We modelled the net cost of adding resistance testing for patients with virological failure and retaining patients without resistance on first-line therapy, rather than switching all failures to second-line therapy...
  3. pmc Association between HIV replication and serum leptin levels: an observational study of a cohort of HIV-1-infected South African women
    Livio Azzoni
    The Wistar Institute, Philadelphia, PA, USA
    J Int AIDS Soc 13:33. 2010
    ..Advanced HIV infection can result in lipoatrophy and wasting, even in the absence of ongoing opportunistic infections, suggesting that HIV may directly affect adipose tissue amount and distribution...
  4. pmc Utility of clinical parameters to identify HIV infection in infants below ten weeks of age in South Africa: a prospective cohort study
    Heather B Jaspan
    Children s Infectious Diseases Clinical Research Unit, Department of Paediatrics and Child Health and Centre for Infectious Diseases, Stellenbosch University, Tygerberg, South Africa
    BMC Pediatr 11:104. 2011
    ..Receiver Operating Characteristic (ROC) curves were compiled using combinations of clinical findings...
  5. pmc A comparative analysis of HIV drug resistance interpretation based on short reverse transcriptase sequences versus full sequences
    Kim Steegen
    Department of Infectious Disease and Biomarkers, Tibotec Virco Virology BVBA, Beerse, Belgium
    AIDS Res Ther 7:38. 2010
    ..abstract:..
  6. pmc Polychromatic immunophenotypic characterization of T cell profiles among HIV-infected patients experiencing immune reconstitution inflammatory syndrome (IRIS)
    David M Murdoch
    Division of Pulmonary and Critical Care Medicine, Duke University Medical Center, Durham, North Carolina, USA
    AIDS Res Ther 6:16. 2009
    ..CONCLUSION: CD4+ and CD8+ T cell subset maturational phenotypes were heterogeneous among IRIS cases and controls. However, IRIS cases demonstrated significant increases in activation of CD8+ T cell effector subpopulations...
  7. pmc Multi-analyte profiling of ten cytokines in South African HIV-infected patients with Immune Reconstitution Inflammatory Syndrome (IRIS)
    Catherine M Worsley
    Department of Molecular Medicine and Haematology, Faculty of Health Sciences, University of the Witwatersrand and National Health Laboratory Services, Johannesburg, South Africa
    AIDS Res Ther 7:36. 2010
    ..The immune system's role in HIV infected patients is complex with cytokine expression strongly influencing HIV infection and replication...
  8. pmc Ultra-high-throughput, automated nucleic acid detection of human immunodeficiency virus (HIV) for infant infection diagnosis using the Gen-Probe Aptima HIV-1 screening assay
    Wendy S Stevens
    Department of Molecular Medicine and Haematology, University of the Witwatersrand, Parktown, Johannesburg, South Africa
    J Clin Microbiol 47:2465-9. 2009
    ..The ability to process 1,900 samples in a 24-h period on the Tigris instrument makes the Aptima assay an attractive option for high-volume, centralized laboratories...
  9. doi request reprint Challenges in implementing HIV load testing in South Africa
    Wendy S Stevens
    Department of Molecular Medicine and Haematology, National Health Laboratory Service and University of the Witwatersrand, Johannesburg, South Africa
    J Infect Dis 201:S78-84. 2010
    ..The development of appropriate solutions for each laboratory tier in South Africa will require close collaboration between researchers in the field and partners in industry...
  10. doi request reprint Quantifying HIV for monitoring antiretroviral therapy in resource-poor settings
    Wendy S Stevens
    Department of Molecular Medicine and Haematology, University of Witwatersrand, Johannesburg, South Africa
    J Infect Dis 201:S16-26. 2010
    ..The field is rapidly changing as new technology platforms are made available for evaluation. This article presents an up to date summary of the assays available for viral load monitoring and suggests approaches for their implementation...
  11. pmc Performance of a novel human immunodeficiency virus (HIV) type 1 total nucleic acid-based real-time PCR assay using whole blood and dried blood spots for diagnosis of HIV in infants
    Wendy Stevens
    University of the Witwatersrand and the National Health Laboratory Services, Johannesburg 2000, South Africa
    J Clin Microbiol 46:3941-5. 2008
    ..The Cobas AmpliPrep/Cobas TaqMan HIV-1 Qual test will support early, reliable diagnosis of HIV in children in routine laboratory settings...
  12. ncbi request reprint HIV/AIDS prevalence testing--merits, methodology and outcomes of a survey conducted at a large mining organisation in South Africa
    W Stevens
    Department of Molecular Medicine and Haematology, University of the Witwatersrand, Johannesburg
    S Afr Med J 96:134-9. 2006
    ..To determine the HIV prevalence rate at Anglo Platinum, a large, multinational organisation operating in South Africa (Gauteng, Limpopo and North-West), and to assess the merits, methodology and outcomes of the survey...
  13. ncbi request reprint Good clinical laboratory practice (GCLP): the need for a hybrid of good laboratory practice and good clinical practice guidelines/standards for medical testing laboratories conducting clinical trials in developing countries
    W Stevens
    Department of Molecular Medicine and Hematology, University of Witwatersrand and National Health Laboratory Service, Saxonwold, South Africa
    Qual Assur 10:83-9. 2003
    ..A specific set of minimum standards or requirements for practical implementation of clinical trial requirements in medical testing laboratories in the developing world is urgently required...
  14. ncbi request reprint Evaluation of the NucliSens EasyQ assay in HIV-1-infected individuals in South Africa
    W Stevens
    Department of Molecular Medicine and Haematology, School of Pathology, Faculty of Health Sciences, University of Witwatersrand and the National Health Laboratory Service, South Africa
    J Virol Methods 124:105-10. 2005
    ..Factors considered during this evaluation included time taken to perform the assay, volume requirements, number of required repeats, potential for contamination...
  15. pmc Evaluation of two commercially available, inexpensive alternative assays used for assessing viral load in a cohort of human immunodeficiency virus type 1 subtype C-infected patients from South Africa
    G Stevens
    Department of Molecular Medicine and Haematology, School of Pathology, University of the Witwatersrand Medical School, Faculty of Health Science and the NHLS, Johannesburg 2193, South Africa
    J Clin Microbiol 43:857-61. 2005
    ..However, both assays provide a less expensive alternative to the Roche viral load assay and demonstrate the same trends during treatment...
  16. doi request reprint Genotypic characterization and comparison of full-length envelope glycoproteins from South African HIV type 1 subtype C primary isolates that utilize CCR5 and/or CXCR4
    Katherine Michler
    HIV Pathogenesis Research Laboratory, Department of Molecular Medicine and Haematology, University of the Witwatersrand Medical School, Parktown, Johannesburg, 2193, South Africa
    AIDS Res Hum Retroviruses 24:743-51. 2008
    ..Further correlation of the env genotype to functionally relevant motifs is necessary to elucidate the relationship between biologically and immunologically relevant sites and aid vaccine and novel drug design...
  17. doi request reprint Emergence of X4 usage among HIV-1 subtype C: evidence for an evolving epidemic in South Africa
    Bridgette J Connell
    HIV Pathogenesis Research Laboratory, Department of Molecular Medicine and Haematology, University of Witwatersrand Medical School, Johannesburg, South Africa
    AIDS 22:896-9. 2008
    ..Results imply that the frequency of CXCR4-utilizing subtype C is increasing with time...
  18. ncbi request reprint Dried blood spots improve access to HIV diagnosis and care for infants in low-resource settings
    Gayle G Sherman
    Department of Molecular Medicine and Haematology, National Health Laboratory Service and University of the Witwatersrand, Johannesburg, South Africa
    J Acquir Immune Defic Syndr 38:615-7. 2005
    ..The Roche Amplicor assay yielded a sensitivity of 100% and a specificity of 99.6%. HIV DNA PCR tests on dried blood spots have the potential to improve health care delivery to HIV-affected children in low-resource settings right now...
  19. doi request reprint Natural polymorphisms of integrase among HIV type 1-infected South African patients
    Muhammad Q Fish
    HIV Pathogenesis Research Laboratory, Department of Molecular Medicine and Haematology, University of Witwatersrand Medical School, Parktown, Johannesburg, South Africa
    AIDS Res Hum Retroviruses 26:489-93. 2010
    ..However, the impact of E157Q and other naturally occurring polymorphisms warrants further phenotypic investigation...
  20. pmc Evaluation of dried whole blood spots obtained by heel or finger stick as an alternative to venous blood for diagnosis of human immunodeficiency virus type 1 infection in vertically exposed infants in the routine diagnostic laboratory
    Janet C Patton
    Wits Pediatric HIV Clinics, Wits Health Consortium, University of the Witwatersrand, Johannesburg, Gauteng, South Africa
    Clin Vaccine Immunol 14:201-3. 2007
    ..The methodologies transfer successfully from the labor-intensive research laboratory to the high-throughput automated routine laboratory...
  21. ncbi request reprint Low risk of contamination with automated and manual excision of dried blood spots for HIV DNA PCR testing in the routine laboratory
    Glenn A Driver
    Wits Paediatric HIV Clinics, Wits Health Consortium, 8 Blackwood Avenue, Parktown, Johannesburg, South Africa
    J Virol Methods 146:397-400. 2007
    ....
  22. pmc Ultrasensitive human immunodeficiency virus type 1 p24 antigen assay modified for use on dried whole-blood spots as a reliable, affordable test for infant diagnosis
    Janet C Patton
    Wits Paediatric HIV Unit, Wits Health Consortium, University of the Witwatersrand, 7 York Rd, Parktown 2193, Johannesburg, South Africa
    Clin Vaccine Immunol 13:152-5. 2006
    ..1 filter paper. The modified assay was found to be reliable and accurate, making it an affordable tool for pediatric HIV diagnosis in developing countries...
  23. doi request reprint Characterization of Lymphomas in a high prevalence HIV setting
    Hamakwa Mantina
    Department of Molecular Medicine and Haematology, Johannesburg, South Africa
    J Acquir Immune Defic Syndr 53:656-60. 2010
    ..HIV infection has been associated with an increased risk of malignancy, both AIDS defining and non-AIDS defining...
  24. doi request reprint Emergence of drug resistance in HIV-1 subtype C infected children failing the South African national antiretroviral roll-out program
    Carole L Wallis
    Department of Molecular Medicine and Haematology, Wits Medical School, University of the Witwatersrand, Parktown, South Africa
    Pediatr Infect Dis J 28:1123-5. 2009
    ..Ninety-eight percent presented with known drug resistance mutations with M184V (82%) and K103N (44%), the dominant mutations...
  25. doi request reprint Changing pattern of lymphoma subgroups at a tertiary academic complex in a high-prevalence HIV setting: a South African perspective
    Tracey M Wiggill
    Departments of Molecular Medicine and Haematology, National Health Laboratory Service and University of the Witwatersrand, Johannesburg, South Africa
    J Acquir Immune Defic Syndr 56:460-6. 2011
    ..Despite the high burden of HIV infection in sub-Saharan regions, published data on HIV and malignancies are sparse from these areas...
  26. pmc Performance evaluation of the Pima™ point-of-care CD4 analyser using capillary blood sampling in field tests in South Africa
    Deborah K Glencross
    Department of Molecular Medicine and Haematology, Faculty of Health Sciences, University of the Witwatersrand, 7 York Road, Parktown, 2198, Johannesburg, South Africa
    J Int AIDS Soc 15:3. 2012
    ..This study evaluated performance of capillary blood sampling using the point-of-care Pima™ CD4 device in representative primary health care clinics doing HIV testing...
  27. ncbi request reprint Affordable diagnosis of human immunodeficiency virus infection in infants by p24 antigen detection
    Gayle G Sherman
    Department of Molecular Medicine and Haematology, National Health Laboratory Service, University of the Witwatersrand, Johannesburg, South Africa
    Pediatr Infect Dis J 23:173-6. 2004
    ..A sensitivity of 98.1% and specificity of 98.7% was obtained testing 203 samples from 24 HIV-infected and 66 uninfected infants born to HIV subtype C-infected women...
  28. pmc HIV-1 phenotypic reverse transcriptase inhibitor drug resistance test interpretation is not dependent on the subtype of the virus backbone
    Michelle Bronze
    Department of Molecular Medicine and Haematology, University of the Witwatersrand, Johannesburg, South Africa
    PLoS ONE 7:e34708. 2012
    ..In conclusion the HXB2 virus backbone remains an acceptable vector for phenotyping HIV-1 subtype C pol amplicons...
  29. pmc Abbott RealTime HIV-1 m2000rt viral load testing: manual extraction versus the automated m2000sp extraction
    Lesley E Scott
    University of the Witwatersrand, Department of Molecular Medicine and Haematology, School of Pathology, Faculty of Health Science, 7 York Rd Parktown, Johannesburg 2000, South Africa
    J Virol Methods 172:78-80. 2011
    ..Overall, this study found no clinically relevant differences in viral load, if samples were extracted manually...
  30. pmc Evaluation of the performance of the automated NucliSENS easyMAG and EasyQ systems versus the Roche AmpliPrep-AMPLICOR combination for high-throughput monitoring of human immunodeficiency virus load
    W Stevens
    Department of Molecular Medicine and Hematology, University of the Witwatersrand and the National Health Laboratory Service, Johannesburg, South Africa
    J Clin Microbiol 45:1244-9. 2007
    ..Additional negative controls have been added to the kit to monitor for contamination based on the South African experience. This assay thus presents a real option for monitoring HIV load assays in high-volume testing environments...
  31. pmc FOXP3 expression is upregulated in CD4T cells in progressive HIV-1 infection and is a marker of disease severity
    Melinda S Suchard
    Haematology and Molecular Medicine, National Health Laboratory Service and University of the Witwatersrand, Johannesburg, South Africa
    PLoS ONE 5:e11762. 2010
    ..To date, it is unclear whether alterations in regulatory T cell (Treg) function are contributory to progression of HIV infection...
  32. doi request reprint African regional external quality assessment for CD4 T-cell enumeration: development, outcomes, and performance of laboratories
    Deborah K Glencross
    Department of Molecular Medicine and Haematology, University of the Witwatersrand, Faculty of Health Sciences and the South African National Health Laboratory Service, Johannesburg, South Africa
    Cytometry B Clin Cytom 74:S69-79. 2008
    ..The overall performance from 2002 to 2006 (Trials 1-20) is reported, together with cumulative longitudinal performance of the different CD4 methods used...
  33. ncbi request reprint Molecular characterization of the HIV type 1 subtype C accessory genes vif, vpr, and vpu
    Catherine M Bell
    HIV Pathogenesis Research Laboratory, Department of Molecular Medicine and Haematology, University of the Witwatersrand Medical School, 7 York Road, Parktown 2193, Johannesburg, South Africa
    AIDS Res Hum Retroviruses 23:322-30. 2007
    ..These results indicate that antiviral drugs that target the conserved functional domains within Vif, Vpr or Vpu could be active against all circulating subtypes, including HIV-1 subtype C...
  34. pmc Early antiretroviral therapy and mortality among HIV-infected infants
    Avy Violari
    Perinatal HIV Research Unit, University of the Witwatersrand, Johannesburg, South Africa
    N Engl J Med 359:2233-44. 2008
    ..We investigated antiretroviral-treatment strategies in the Children with HIV Early Antiretroviral Therapy (CHER) trial...
  35. ncbi request reprint Tuberculosis in children dying with HIV-related lung disease: clinical-pathological correlations
    W P Rennert
    Chris Hani Baragwanath Hospital, Department of Paediatrics, University of the Witwatersrand, Soweto, South Africa
    Int J Tuberc Lung Dis 6:806-13. 2002
    ..Chris Hani Baragwanath Hospital, Soweto, South Africa...
  36. ncbi request reprint Malaria prevention in The Gambia: patterns of expenditure and determinants of demand at the household level
    V Wiseman
    Gates Malaria Partnership, Gates Malaria Partnership and Health Policy Unit, London School of Hygiene and Tropical Medicine, London, UK
    Trop Med Int Health 11:419-31. 2006
    ..To provide a better understanding of (1) the amounts households in The Gambia spend on a wide variety of malaria prevention measures, (2) how expenditure fluctuates throughout the year and (3) the main determinants of expenditure...
  37. ncbi request reprint Risk factors for defaulting from tuberculosis treatment: a prospective cohort study of 301 cases in the Gambia
    P C Hill
    Department of Tuberculosis, Medical Research Council Laboratories, Banjul, The Gambia
    Int J Tuberc Lung Dis 9:1349-54. 2005
    ..An urban tuberculosis (TB) clinic, The Gambia...
  38. ncbi request reprint The challenge of laboratory monitoring of HIV
    D K Glencross
    S Afr Med J 92:248. 2002
  39. ncbi request reprint Kaposi sarcoma-associated herpesvirus/human herpesvirus 8 and multiple myeloma in South Africa
    M Patel
    Haematology Unit, Department of Medicine, Chris Hani Baragwanth Hospital, Soweto, South Africa
    Diagn Mol Pathol 10:95-9. 2001
    ..Taken together, the finding of a positive result in 4 of 17 patients (23.5%), which is similar to the background seroprevalence rate, does not support a clear association between myeloma and KSHV in blacks in South Africa...