D E Rawlings

Summary

Affiliation: University of Stellenbosch
Country: South Africa

Publications

  1. ncbi request reprint Heavy metal mining using microbes
    Douglas E Rawlings
    Department of Microbiology, University of Stellenbosch, Private Bag X1, South Africa
    Annu Rev Microbiol 56:65-91. 2002
  2. ncbi request reprint The evolution of pTF-FC2 and pTC-F14, two related plasmids of the IncQ-family
    Douglas E Rawlings
    Department of Microbiology, Stellenbosch University, Private Bag X1, Matieland 7602, South Africa
    Plasmid 53:137-47. 2005
  3. ncbi request reprint Biomineralization of metal-containing ores and concentrates
    Douglas E Rawlings
    Department of Microbiology, University of Stellenbosch, Private Bag X1, 7602, Matieland, South Africa
    Trends Biotechnol 21:38-44. 2003
  4. pmc Comparative biology of IncQ and IncQ-like plasmids
    D E Rawlings
    Department of Microbiology, University of Stellenbosch, Matieland 7602, South Africa
    Microbiol Mol Biol Rev 65:481-96, table of contents. 2001
  5. pmc Cloning, nucleotide sequence, and enzymatic characterization of an alpha-amylase from the ruminal bacterium Butyrivibrio fibrisolvens H17c
    E Rumbak
    Department of Microbiology, University of Cape Town, South Africa
    J Bacteriol 173:4203-11. 1991
  6. pmc Structure and function of Tn5467, a Tn21-like transposon located on the Thiobacillus ferrooxidans broad-host-range plasmid pTF-FC2
    A M Clennel
    Department of Microbiology, University of Cape Town, Rondebosch, South Africa
    Appl Environ Microbiol 61:4223-9. 1995
  7. ncbi request reprint Proteic toxin-antitoxin, bacterial plasmid addiction systems and their evolution with special reference to the pas system of pTF-FC2
    D E Rawlings
    Department of Microbiology, University of Stellenbosch, Matieland, South Africa
    FEMS Microbiol Lett 176:269-77. 1999
  8. pmc The chromosomal arsenic resistance genes of Thiobacillus ferrooxidans have an unusual arrangement and confer increased arsenic and antimony resistance to Escherichia coli
    B G Butcher
    Department of Microbiology, University of Stellenbosch, Matieland, South Africa 7602
    Appl Environ Microbiol 66:1826-33. 2000
  9. pmc A Tn7-like transposon is present in the glmUS region of the obligately chemoautolithotrophic bacterium Thiobacillus ferrooxidans
    J C Oppon
    Department of Microbiology, University of Cape Town, Rondebosch 7700, South Africa
    J Bacteriol 180:3007-12. 1998
  10. pmc Isolation of a new broad-host-range IncQ-like plasmid, pTC-F14, from the acidophilic bacterium Acidithiobacillus caldus and analysis of the plasmid replicon
    M N Gardner
    Department of Microbiology, University of Stellenbosch, Matieland 7602, South Africa
    J Bacteriol 183:3303-9. 2001

Collaborators

  • S M Deane
  • D B Johnson
  • B P Rosen
  • I Marla Tuffin
  • Wesley Loftie-Eaton
  • Leonardo J van Zyl
  • Nicolette J Coram
  • Gwynneth F Matcher
  • Leonard J van Zyl
  • Jie Qin
  • Andre A Kotze
  • Murray N Gardner
  • M N Gardner
  • B G Butcher
  • Lilly Ann Louw
  • Jolanda M van Munster
  • Jun Ye
  • Timothy L Stemmler
  • Hsueh Liang Fu
  • Krisztina Z Bencze
  • A S Smith
  • J C Oppon
  • Stanton B Hector
  • Peter de Groot
  • C N Dominy
  • A M Clennel
  • N L Craig
  • R J Sarnovsky
  • B Johnston
  • E Rumbak
  • D R Woods
  • G G Lindsey

Detail Information

Publications29

  1. ncbi request reprint Heavy metal mining using microbes
    Douglas E Rawlings
    Department of Microbiology, University of Stellenbosch, Private Bag X1, South Africa
    Annu Rev Microbiol 56:65-91. 2002
    ..The development of higher-temperature processes will extend the variety of minerals that can be commercially processed...
  2. ncbi request reprint The evolution of pTF-FC2 and pTC-F14, two related plasmids of the IncQ-family
    Douglas E Rawlings
    Department of Microbiology, Stellenbosch University, Private Bag X1, Matieland 7602, South Africa
    Plasmid 53:137-47. 2005
    ..Other aspects of the evolution of the two plasmids are described and the danger of making the assumption that incompatibly of IncQ-like plasmids is a reflection of the degree of relatedness of two plasmids is discussed...
  3. ncbi request reprint Biomineralization of metal-containing ores and concentrates
    Douglas E Rawlings
    Department of Microbiology, University of Stellenbosch, Private Bag X1, 7602, Matieland, South Africa
    Trends Biotechnol 21:38-44. 2003
    ....
  4. pmc Comparative biology of IncQ and IncQ-like plasmids
    D E Rawlings
    Department of Microbiology, University of Stellenbosch, Matieland 7602, South Africa
    Microbiol Mol Biol Rev 65:481-96, table of contents. 2001
    ..This review covers the ecology, classification, and evolution of IncQ and IncQ-like plasmids...
  5. pmc Cloning, nucleotide sequence, and enzymatic characterization of an alpha-amylase from the ruminal bacterium Butyrivibrio fibrisolvens H17c
    E Rumbak
    Department of Microbiology, University of Cape Town, South Africa
    J Bacteriol 173:4203-11. 1991
    ..Maltotriose was the major initial hydrolysis product from starch, although extended incubation resulted in its hydrolysis to maltose and glucose...
  6. pmc Structure and function of Tn5467, a Tn21-like transposon located on the Thiobacillus ferrooxidans broad-host-range plasmid pTF-FC2
    A M Clennel
    Department of Microbiology, University of Cape Town, Rondebosch, South Africa
    Appl Environ Microbiol 61:4223-9. 1995
    ..Tn5467 appears to be a defective transposon which belongs to the Tn21 subgroup of the Tn3 family...
  7. ncbi request reprint Proteic toxin-antitoxin, bacterial plasmid addiction systems and their evolution with special reference to the pas system of pTF-FC2
    D E Rawlings
    Department of Microbiology, University of Stellenbosch, Matieland, South Africa
    FEMS Microbiol Lett 176:269-77. 1999
    ..Recent findings regarding toxin-antitoxin systems and questions that have arisen as a result of these findings are reviewed...
  8. pmc The chromosomal arsenic resistance genes of Thiobacillus ferrooxidans have an unusual arrangement and confer increased arsenic and antimony resistance to Escherichia coli
    B G Butcher
    Department of Microbiology, University of Stellenbosch, Matieland, South Africa 7602
    Appl Environ Microbiol 66:1826-33. 2000
    ..This divergent orientation was conserved in the four T. ferrooxidans strains investigated...
  9. pmc A Tn7-like transposon is present in the glmUS region of the obligately chemoautolithotrophic bacterium Thiobacillus ferrooxidans
    J C Oppon
    Department of Microbiology, University of Cape Town, Rondebosch 7700, South Africa
    J Bacteriol 180:3007-12. 1998
    ..coli is a heterotroph, ancestors of the Tn7-like transposons must have been active in a variety of physiologically different bacteria so that their descendants are now found in bacteria that occupy very different ecological niches...
  10. pmc Isolation of a new broad-host-range IncQ-like plasmid, pTC-F14, from the acidophilic bacterium Acidithiobacillus caldus and analysis of the plasmid replicon
    M N Gardner
    Department of Microbiology, University of Stellenbosch, Matieland 7602, South Africa
    J Bacteriol 183:3303-9. 2001
    ..This is the second time a plasmid stability system of this type has been found on an IncQ-like plasmid...
  11. pmc Efficiency of the pTF-FC2 pas poison-antidote stability system in Escherichia coli is affected by the host strain, and antidote degradation requires the lon protease
    A S Smith
    Department of Microbiology, University of Cape Town, Rondebosch 7700, South Africa
    J Bacteriol 180:5458-62. 1998
    ..A decrease in host cell death following plasmid loss from an E. coli lon mutant and a decrease in plasmid stability suggested that the Lon protease plays a role in the rate of turnover of PasA antidote...
  12. pmc Characterization of the Butyrivibrio fibrisolvens glgB gene, which encodes a glycogen-branching enzyme with starch-clearing activity
    E Rumbak
    Department of Microbiology, University of Cape Town, South Africa
    J Bacteriol 173:6732-41. 1991
    ..The enzymatic properties of the purified enzyme were investigated. The enzyme transferred chains of 5 to 10 (optimum, 7) glucose units, using amylose and amylopetin as substrates, to produce a highly branched polymer...
  13. pmc Resistance determinants of a highly arsenic-resistant strain of Leptospirillum ferriphilum isolated from a commercial biooxidation tank
    I Marla Tuffin
    Department of Microbiology, Stellenbosch University, Private Bag X1, Matieland 7602, South Africa
    Appl Environ Microbiol 72:2247-53. 2006
    ..The second set of arsenic resistance genes conferred very low levels of resistance in E. coli and appeared to be poorly expressed in both L. ferriphilum and E. coli...
  14. ncbi request reprint The microbiology of biomining: development and optimization of mineral-oxidizing microbial consortia
    Douglas E Rawlings
    Department of Microbiology, University of Stellenbosch, Private Bag X1, Matieland 7602, South Africa
    Microbiology 153:315-24. 2007
    ..This review discusses theoretical and pragmatic aspects of assembling microbial consortia to process different mineral ores and concentrates, and the challenges for using constructed consortia in non-sterile industrial-scale operations...
  15. pmc Two large, related, cryptic plasmids from geographically distinct isolates of Sulfobacillus thermotolerans
    S M Deane
    Department of Microbiology, University of Stellenbosch, Private Bag X1, Matieland 7602, South Africa
    Appl Environ Microbiol 77:8175-80. 2011
    ..This analysis revealed a common "backbone" region coding for a potential plasmid stability system plus a nonpheromone conjugation system containing homologues of both type IV and type II (tight adherence, or Tad-like) secretion systems...
  16. ncbi request reprint Evolution of compatible replicons of the related IncQ-like plasmids, pTC-F14 and pTF-FC2
    Murray N Gardner
    Department of Microbiology, University of Stellenbosch, Private Bag X1, Matieland 7602, South Africa
    Microbiology 150:1797-808. 2004
    ..However, the RepA and RepB proteins have the ability to function with either replicon...
  17. doi request reprint The effect of the location of the proteic post-segregational stability system within the replicon of plasmid pTF-FC2 on the fine regulation of plasmid replication
    Gwynneth F Matcher
    Department of Microbiology, University of Stellenbosch, Matieland, South Africa
    Plasmid 62:98-107. 2009
    ....
  18. pmc Presence of a family of plasmids (29 to 65 kilobases) with a 26-kilobase common region in different strains of the sulfur-oxidizing bacterium Acidithiobacillus caldus
    Leonard J van Zyl
    Department of Microbiology, University of Stellenbosch, Private Bag X1, Matieland 7602, South Africa
    Appl Environ Microbiol 74:4300-8. 2008
    ..Genes coding for arsenic resistance, plasmid mobilization, plasmid stability, and a putative restriction-modification system occur within these mosaic regions...
  19. pmc Plasmid evolution and interaction between the plasmid addiction stability systems of two related broad-host-range IncQ-like plasmids
    Shelly M Deane
    Department of Microbiology, University of Stellenbosch, Matieland 7602, South Africa
    J Bacteriol 186:2123-33. 2004
    ..coli host cell. Plasmid curing resulted in the arrest of cell growth but did not cause cell death, and plasmid stability was not influenced by the E. coli mazEF genes...
  20. pmc Molecular relationship between two groups of the genus Leptospirillum and the finding that Leptospirillum ferriphilum sp. nov. dominates South African commercial biooxidation tanks that operate at 40 degrees C
    Nicolette J Coram
    Department of Microbiology, University of Stellenbosch, Matieland, 7602, South Africa
    Appl Environ Microbiol 68:838-45. 2002
    ..ferrooxidans), were capable of growth at 45 degrees C. All the leptospirilla isolated from commercial biooxidation tanks in South Africa were from group II...
  21. pmc Isolation, sequence analysis, and comparison of two plasmids (28 and 29 kilobases) from the biomining bacterium Leptospirillum ferrooxidans ATCC 49879
    Nicolette J Coram
    Department of Microbiology, University of Stellenbosch, Private Bag X1, Matieland 7602, South Africa
    Appl Environ Microbiol 71:7515-22. 2005
    ..Twenty-four ORFs had products with no homologues in the GenBank/NCBI database. An analysis of the ORFs and other features of the two plasmids, the first to be isolated from a bacterium of the genus Leptospirillum, is presented...
  22. ncbi request reprint A geographically widespread plasmid from Thiobacillus ferrooxidans has genes for ferredoxin-, FNR-, prismane- and NADH-oxidoreductase-like proteins which are also located on the chromosome
    C N Dominy
    Department of Microbiology, University of Cape Town, South Africa
    Microbiology 143:3123-36. 1997
    ..This region contained no ORFs and was identical to that previously reported for the replicon of a 9.8 kb T. ferrooxidans plasmid, pTF191...
  23. pmc Construction of arsB and tetH mutants of the sulfur-oxidizing bacterium Acidithiobacillus caldus by marker exchange
    Leonardo J van Zyl
    Department of Microbiology, University of Stellenbosch, Private Bag X1, Matieland 7602, South Africa
    Appl Environ Microbiol 74:5686-94. 2008
    ..caldus arsB and tetH mutants, and the effects of these mutations on cell growth in the presence of arsenic and by means of tetrathionate oxidation were demonstrated...
  24. pmc Comparative biology of two natural variants of the IncQ-2 family plasmids, pRAS3.1 and pRAS3.2
    Wesley Loftie-Eaton
    Department of Microbiology, University of Stellenbosch, Private Bag X1, Matieland 7602, South Africa
    J Bacteriol 191:6436-46. 2009
    ..We propose that the pRAS3 plasmids represent a third, gamma incompatibility group within the IncQ-2 family plasmids...
  25. ncbi request reprint Cloning and characterization of the chromosomal arsenic resistance genes from Acidithiobacillus caldus and enhanced arsenic resistance on conjugal transfer of ars genes located on transposon TnAtcArs
    Andre A Kotze
    Department of Microbiology, University of Stellenbosch, Private Bag X1, Matieland 7602, South Africa
    Microbiology 152:3551-60. 2006
    ..caldus ars genes of chromosomal origin. Plasmid pSa-mediated conjugal transfer of TnAtcArs from E. coli to At. caldus strains lacking the transposon was successful, and resulted in greatly increased levels of resistance to arsenite...
  26. ncbi request reprint An unusual Tn21-like transposon containing an ars operon is present in highly arsenic-resistant strains of the biomining bacterium Acidithiobacillus caldus
    I Marla Tuffin
    Department of Microbiology, Stellenbosch University, Private Bag X1, Matieland 7602, South Africa
    Microbiology 151:3027-39. 2005
    ....
  27. pmc Analysis of the mobilization region of the broad-host-range IncQ-like plasmid pTC-F14 and its ability to interact with a related plasmid, pTF-FC2
    Leonardo J van Zyl
    Department of Microbiology, University of Stellenbosch, Matieland 7600, South Africa
    J Bacteriol 185:6104-11. 2003
    ..No evidence for any negative effect on the transfer of one plasmid by the related, potentially competitive plasmid was obtained...
  28. pmc Evolutionary competitiveness of two natural variants of the IncQ-like plasmids, pRAS3.1 and pRAS3.2
    Wesley Loftie-Eaton
    Department of Microbiology, University of Stellenbosch, Private Bag X1, Matieland, South Africa
    J Bacteriol 192:6182-90. 2010
    ..8% less of a metabolic burden on an E. coli host population, and therefore, pRAS3.2 has a competitive advantage over pRAS3.1 at the population level, as pRAS3.2-containing cells would be expected to outgrow pRAS3.1-containing cells...
  29. pmc Convergent evolution of a new arsenic binding site in the ArsR/SmtB family of metalloregulators
    Jie Qin
    Department of Biochemistry and Molecular Biology, Wayne State University, School of Medicine, Detroit, MI 48201, USA
    J Biol Chem 282:34346-55. 2007
    ..These results suggest that the As(III)-S(3) binding sites in AfArsR and R773 ArsR arose independently at spatially distinct locations in their three-dimensional structures...