Mark Patrick Nicol
Affiliation: University of Cape Town
Country: South Africa
- Rapid genotypic assays to identify drug-resistant Mycobacterium tuberculosis in South AfricaJoanna Evans
Division of Medical Microbiology, Institute of Infectious Diseases and Molecular Medicine, University of Cape Town, Cape Town, South Africa
J Antimicrob Chemother 63:11-6. 2009..To evaluate the efficacy of such assays in this setting, the GenoType MTBDRplus assay (HAIN Lifescience) and multiplex allele-specific PCR assays were carried out...
- Determine TB-LAM lateral flow urine antigen assay for HIV-associated tuberculosis: recommendations on the design and reporting of clinical studiesStephen D Lawn
Department of Clinical Research, Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK
BMC Infect Dis 13:407. 2013..Finally, we consider how the assay could be used in combination with other assays and outline the types of studies that are required to build the evidence base concerning its use. ..
- Modern lineages of Mycobacterium tuberculosis exhibit lineage-specific patterns of growth and cytokine induction in human monocyte-derived macrophagesRajesh Sarkar
Division of Medical Microbiology, University of Cape Town, Cape Town, South Africa
PLoS ONE 7:e43170. 2012..There is, however, little information available as to whether these patterns of immune subversion are features of individual strains or characteristic of broad clonal lineages of M. tuberculosis...
- Advances in the diagnosis of pulmonary tuberculosis in HIV-infected and HIV-uninfected childrenTom G Connell
Infectious Diseases Unit, Department of General Medicine and Murdoch Children s Research Institute, Royal Children s Hospital, Melbourne, Australia
J Infect Dis 204:S1151-8. 2011..This review will focus on a number of recent developments for the diagnosis of tuberculosis, with a specific emphasis on the application of these new tests to children in settings where tuberculosis is endemic...
- Impact of a novel molecular TB diagnostic system in patients at high risk of TB mortality in rural South Africa (Uchwepheshe): study protocol for a cluster randomised trialRichard J Lessells
Department of Clinical Research, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK
Trials 14:170. 2013..We present a cluster randomised trial designed to evaluate whether the positioning of this diagnostic system within the health system has an impact on important patient-level outcomes...
- Xpert MTB/RIF testing of stool samples for the diagnosis of pulmonary tuberculosis in childrenMark P Nicol
Division of Medical Microbiology and Institute for Infectious Diseases and Molecular Medicine, University of Cape Town, and National Health Laboratory Service of South Africa, South Africa
Clin Infect Dis 57:e18-21. 2013..Sputum Xpert detected 11/17 (65%) cases. Stool holds promise for PTB diagnosis in HIV-infected children. ..
- High levels of multidrug resistant tuberculosis in new and treatment-failure patients from the Revised National Tuberculosis Control Programme in an urban metropolis (Mumbai) in Western IndiaDesiree T B D'souza
The Foundation for Medical Research, 84 A, R, G, Thadani Marg, Worli, Mumbai 400 018, India
BMC Public Health 9:211. 2009..Drug susceptibility testing (DST) is not routinely performed as a part of programme policy and public health laboratory infrastructure, is limited and poorly equipped to cope with large scale testing...
- Reversion and conversion of Mycobacterium tuberculosis IFN-gamma ELISpot results during anti-tuberculous treatment in HIV-infected childrenTom G Connell
Institute of Infectious Diseases and Molecular Medicine, University of Cape Town, Cape Town, South Africa
BMC Infect Dis 10:138. 2010..The kinetics of IFN-gamma responses to Mycobacterium tuberculosis (MTB) antigens in HIV-infected children during treatment have not however been previously investigated...
- Enhanced anti-mycobacterial immunity in children with erythema nodosum and a positive tuberculin skin testMark P Nicol
School of Child and Adolescent Health, Red Cross Children s Hospital, University of Cape Town, Cape Town, Western Cape, South Africa
J Invest Dermatol 127:2152-7. 2007..Children presenting with EN and a positive TST show evidence of responses associated with enhanced anti-mycobacterial immunity...
- Accuracy of the Xpert MTB/RIF test for the diagnosis of pulmonary tuberculosis in children admitted to hospital in Cape Town, South Africa: a descriptive studyMark P Nicol
Division of Medical Microbiology and Institute for Infectious Diseases and Molecular Medicine, University of Cape Town, Cape Town, South Africa
Lancet Infect Dis 11:819-24. 2011..We aimed to assess the accuracy of the test for the diagnosis of pulmonary tuberculosis in children in an area with high tuberculosis and HIV prevalences...
- New specimens and laboratory diagnostics for childhood pulmonary TB: progress and prospectsMark P Nicol
Division of Medical Microbiology, Department of Clinical Laboratory Sciences, Institute for Infectious Diseases and Molecular Medicine, University of Cape Town and National Health Laboratory Service of South Africa
Paediatr Respir Rev 12:16-21. 2011..The recent development of an integrated specimen processing and real-time PCR testing platform for M. tuberculosis and rifampicin resistance is an important advance that requires evaluation in childhood TB...
- Comparison of T-SPOT.TB assay and tuberculin skin test for the evaluation of young children at high risk for tuberculosis in a community settingMark P Nicol
School of Child and Adolescent Health, University of Cape Town, Cape Town, South Africa
Pediatrics 123:38-43. 2009..TB; Oxford Immunotec, Oxford, United Kingdom) and the tuberculin skin test for the detection of tuberculosis infection in very young children being evaluated for active tuberculosis in a rural community setting...
- MVA-85A, a novel candidate booster vaccine for the prevention of tuberculosis in children and adultsMark Patrick Nicol
University of Cape Town, Institute of Infectious Diseases and Molecular Medicine, South Africa
Curr Opin Mol Ther 12:124-34. 2010....
- The clinical consequences of strain diversity in Mycobacterium tuberculosisMark P Nicol
Institute of Infectious Diseases and Molecular Medicine, Faculty of Health Sciences, University of Cape Town, Anzio Road, Observatory, 7925, Cape Town, South Africa
Trans R Soc Trop Med Hyg 102:955-65. 2008..tuberculosis, based on increased availability of sequence data from the major strain lineages, will allow a structured approach to understand further the consequences of strain diversity in M. tuberculosis...
- Detection of tuberculosis in HIV-infected children using an enzyme-linked immunospot assayMary Ann Davies
Red Cross Children s Hospital, School of Child and Adolescent Health, University of Cape Town, Cape Town, South Africa
AIDS 23:961-9. 2009..To evaluate an enzyme-linked immunospot assay (ELISPOT) for the diagnosis of tuberculosis (TB) in HIV-infected children with suspected TB and to compare the performance of ELISPOT with the tuberculin skin test (TST)...
- Distribution of strain families of Mycobacterium tuberculosis causing pulmonary and extrapulmonary disease in hospitalized children in Cape Town, South AfricaMark P Nicol
Institute of Infectious Disease and Molecular Medicine, Faculty of Health Sciences, University of Cape Town, Anzio Road, Observatory 7925, Cape Town, South Africa
J Clin Microbiol 43:5779-81. 2005..Two strain families, LAM3/F11 and W-Beijing, predominated; but there was no overall association with extrapulmonary disease...
- Recent and rapid emergence of W-Beijing strains of Mycobacterium tuberculosis in Cape Town, South AfricaDonna Cowley
Institute of Infectious Diseases and Molecular Medicine, University of Cape Town, Cape Town, South Africa
Clin Infect Dis 47:1252-9. 2008..Our goal was to determine whether W-Beijing strains are expanding in a region of South Africa where rates of tuberculosis are among the highest in the world...
- Type 1 helper T cells and FoxP3-positive T cells in HIV-tuberculosis-associated immune reconstitution inflammatory syndromeGraeme Meintjes
F R C P, Institute of Infectious Diseases and Molecular Medicine, University of Cape Town, Observatory 7925, South Africa
Am J Respir Crit Care Med 178:1083-9. 2008..Tuberculosis-associated immune reconstitution inflammatory syndrome (TB-IRIS) induced by combination antiretroviral therapy (cART) has been attributed to dysregulated expansion of tuberculin PPD-specific IFN-gamma-secreting CD4(+) T cells...
- Reconstitution of antimycobacterial immune responses in HIV-infected children receiving HAARTBeate Kampmann
School of Child and Adolescent Health, University of Cape Town, and Red Cross Children s Hospital, Rondebosch, Cape Town 7701, Republic of South Africa
AIDS 20:1011-8. 2006..We investigated whether changes in mycobacterial-specific immune responses can be demonstrated in children after commencing antiretroviral therapy...
- Failure to control growth of mycobacteria in blood from children infected with human immunodeficiency virus and its relationship to T cell functionGwen N Tena
School of Child and Adolescent Health, Red Cross Children s Hospital, University of Cape Town, Cape Town, Republic of South Africa
J Infect Dis 187:1544-51. 2003..Impaired control of infection was not reconstituted by the addition of exogenous IFN-gamma. The whole-blood assay provides an important tool for monitoring and dissecting of human immune responses to mycobacterial infection...
- A deletion defining a common Asian lineage of Mycobacterium tuberculosis associates with immune subversionSandra M Newton
Wellcome Trust Center for Research in Clinical Tropical Medicine, Center for Molecular Microbiology and Infection, and Kennedy Institute of Rheumatology, Faculty of Medicine, Imperial College London, London W2 1PG, United Kingdom
Proc Natl Acad Sci U S A 103:15594-8. 2006..The Rv1519 polymorphism in M. tuberculosis confers an immune subverting phenotype that contributes to the persistence and outbreak potential of this lineage...
- Transmission of Mycobacterium tuberculosis undetected by tuberculin skin testingSuzanne T Anderson
M B B S, Department of Pediatrics, Division of Medicine, Wright Fleming Institute, Imperial College London, London W2 1PG, UK
Am J Respir Crit Care Med 173:1038-42. 2006..The development of tuberculin skin test (TST) positivity following infection by Mycobacterium tuberculosis is not invariable and may depend on bacillary as well as host factors...
- Evidence guiding the treatment of children with mycobacterial diseasesMark P Nicol
Clin Infect Dis 44:1065-6. 2007