A I Louw
Affiliation: University of Pretoria
Country: South Africa
- Heterologous expression of plasmodial proteins for structural studies and functional annotationLyn Marie Birkholtz
Department of Biochemistry, University of Pretoria, Pretoria, South Africa
Malar J 7:197. 2008..Here the status quo of heterologous production of plasmodial proteins is presented, constraints are highlighted and alternative strategies and hosts for functional expression and annotation of plasmodial proteins are reviewed...
- Deletion mutagenesis of large areas in Plasmodium falciparum genes: a comparative studyMarni Williams
Department of Biochemistry, School of Biological Sciences, Faculty of Natural and Agricultural Sciences, University of Pretoria, Pretoria, South Africa
Malar J 6:64. 2007..A comparative study of five widely used PCR-based mutagenesis methods identified a modified inverse PCR method as particularly suitable for the deletion of large areas (>100 bp) in malaria parasite genes...
- Plasmodium falciparum spermidine synthase inhibition results in unique perturbation-specific effects observed on transcript, protein and metabolite levelsJohn V W Becker
CSIR Biosciences, PO Box 395, Pretoria, 0001, South Africa
BMC Genomics 11:235. 2010..falciparum spermidine synthase (PfSpdSyn) were examined on a morphological, transcriptomic, proteomic and metabolic level...
- MADIBA: a web server toolkit for biological interpretation of Plasmodium and plant gene clustersPhilip J Law
Bioinformatics and Computational Biology Unit, African Centre for Gene Technologies ACGT, Department of Biochemistry, Faculty of Natural and Agricultural Sciences, University of Pretoria, Pretoria, 0002, South Africa
BMC Genomics 9:105. 2008..While many algorithms and software have been developed for analysing gene expression, the extraction of relevant information from experimental data is still a substantial challenge, requiring significant time and skill...
- The influence of tick behavior, biotope and host specificity on concerted evolution of the platelet aggregation inhibitor savignygrin, from the soft tick Ornithodoros savignyiB J Mans
Department of Biochemistry, University of Pretoria, Pretoria 0002, South Africa
Insect Biochem Mol Biol 33:623-9. 2003..Our results suggest that environmental factors and host associations do play an important role in the evolution of anti-hemostatic components in ticks...