Affiliation: University of Pretoria
Country: South Africa
Bhoora R, Franssen L, Oosthuizen M, Guthrie A, Zweygarth E, Penzhorn B, et al
. Sequence heterogeneity in the 18S rRNA gene within Theileria equi and Babesia caballi from horses in South Africa. Vet Parasitol. 2009;159:112-20 pubmed publisher
..caballi is required prior to the development of molecular diagnostic tests to detect these parasites in horses. Species-specific probes must be in designed in regions of the gene that are both conserved within and unique to each species...
Bhoora R, Quan M, Franssen L, Butler C, van der Kolk J, Guthrie A, et al
. Development and evaluation of real-time PCR assays for the quantitative detection of Babesia caballi and Theileria equi infections in horses from South Africa. Vet Parasitol. 2010;168:201-11 pubmed publisher
..equi-specific qPCR assay could positively detect T. equi DNA in 80% of the samples. These results suggest that the qPCR assays are more sensitive than the RLB assay for the detection of T. equi and B. caballi infections in field samples...
Bhoora R, Quan M, Matjila P, Zweygarth E, Guthrie A, Collins N. Sequence heterogeneity in the equi merozoite antigen gene (ema-1) of Theileria equi and development of an ema-1-specific TaqMan MGB assay for the detection of T. equi. Vet Parasitol. 2010;172:33-45 pubmed publisher
..It is possible that the different T. equi ema-1 genotypes could be related to antigenic variability and pathogenicity and may be associated with clinical differences in equine piroplasmosis cases, but this remains to be elucidated. ..