Roman Jerala

Summary

Affiliation: National Institute of Chemistry
Country: Slovenia

Publications

  1. Lapenta F, Aupic J, Strmšek Ž, Jerala R. Coiled coil protein origami: from modular design principles towards biotechnological applications. Chem Soc Rev. 2018;47:3530-3542 pubmed publisher
    ..The CCPO platform therefore has potential for diverse applications in biomedicine and biotechnology, from drug delivery to molecular cages. ..
  2. Pohar J, Kužnik Krajnik A, Jerala R, Benčina M. Minimal sequence requirements for oligodeoxyribonucleotides activating human TLR9. J Immunol. 2015;194:3901-8 pubmed publisher
    ..On the basis of sequence requirements, we conclude that two binding sites with different affinities for CG are formed in the human TLR9 dimer, with a very stringent binding site interacting with the 5' CpG motif. ..
  3. Manček Keber M, Frank Bertoncelj M, Hafner Bratkovič I, Smole A, Zorko M, Pirher N, et al. Toll-like receptor 4 senses oxidative stress mediated by the oxidation of phospholipids in extracellular vesicles. Sci Signal. 2015;8:ra60 pubmed publisher
    ..Together, these data suggest that EVs act as an oxidative stress-induced endogenous danger signal that underlies the pervasive role of TLR4 in inflammatory diseases. ..
  4. Pohar J, Yamamoto C, Fukui R, Cajnko M, Miyake K, Jerala R, et al. Selectivity of Human TLR9 for Double CpG Motifs and Implications for the Recognition of Genomic DNA. J Immunol. 2017;198:2093-2104 pubmed publisher
  5. Golob Urbanc A, Rajcevic U, Strmšek Ž, Jerala R. Design of split superantigen fusion proteins for cancer immunotherapy. J Biol Chem. 2019;294:6294-6305 pubmed publisher
    ..This design selectively activated T cells by split SEA-scFv fusion binding to target cells. ..
  6. Fink T, Lonzarić J, Praznik A, Plaper T, Merljak E, Leben K, et al. Design of fast proteolysis-based signaling and logic circuits in mammalian cells. Nat Chem Biol. 2019;15:115-122 pubmed publisher
    ..The designed split-protease-cleavable orthogonal-CC-based (SPOC) logic circuits enable response to chemical or biological signals within minutes rather than hours and should be useful for diverse medical and nonmedical applications. ..
  7. Hafner Bratkovic I, Sušjan P, Lainšček D, Tapia Abellán A, Cerović K, Kadunc L, et al. NLRP3 lacking the leucine-rich repeat domain can be fully activated via the canonical inflammasome pathway. Nat Commun. 2018;9:5182 pubmed publisher
    ..This lack of signal amplification is likely a protective mechanism to decrease sensitivity to endogenous triggers to impede autoinflammation. ..
  8. Lebar T, Verbič A, Ljubetic A, Jerala R. Polarized displacement by transcription activator-like effectors for regulatory circuits. Nat Chem Biol. 2019;15:80-87 pubmed publisher
  9. Pohar J, Lainšček D, Fukui R, Yamamoto C, Miyake K, Jerala R, et al. Species-Specific Minimal Sequence Motif for Oligodeoxyribonucleotides Activating Mouse TLR9. J Immunol. 2015;195:4396-405 pubmed publisher

More Information

Publications24

  1. Pohar J, Lainšček D, Kunšek A, Cajnko M, Jerala R, Benčina M. Publisher Correction: Phosphodiester backbone of the CpG motif within immunostimulatory oligodeoxynucleotides augments activation of Toll-like receptor 9. Sci Rep. 2018;8:4269 pubmed publisher
    ..A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper. ..
  2. Lainšček D, Lebar T, Jerala R. Transcription activator-like effector-mediated regulation of gene expression based on the inducible packaging and delivery via designed extracellular vesicles. Biochem Biophys Res Commun. 2017;484:15-20 pubmed publisher
    ..This technology therefore represents an efficient delivery for the TALE-based transcriptional regulators. ..
  3. Vašl J, Oblak A, Peternelj T, Klett J, Martín Santamaría S, Gioannini T, et al. Molecular Basis of the Functional Differences between Soluble Human Versus Murine MD-2: Role of Val135 in Transfer of Lipopolysaccharide from CD14 to MD-2. J Immunol. 2016;196:2309-18 pubmed publisher
    ..We propose that the residue at position 135 of MD-2 governs the dynamics of the binding pocket and its ability to accommodate lipid A, which is allosterically affected by bound TLR4. ..
  4. Ljubetic A, Drobnak I, Gradisar H, Jerala R. Designing the structure and folding pathway of modular topological bionanostructures. Chem Commun (Camb). 2016;52:5220-9 pubmed publisher
    ..This approach opens the way towards incorporation of designed foldamers in biological systems and their functionalization. ..
  5. Lebar T, Jerala R. Designed Transcriptional Regulation in Mammalian Cells Based on TALE- and CRISPR/dCas9. Methods Mol Biol. 2018;1772:191-203 pubmed publisher
    ..In this chapter, we describe the protocols for achieving efficient transcriptional regulation with TALE- and CRISPR-based designed transcription factors in mammalian cells. ..
  6. Lonzarić J, Lebar T, Majerle A, Manček Keber M, Jerala R. Locked and proteolysis-based transcription activator-like effector (TALE) regulation. Nucleic Acids Res. 2016;44:1471-81 pubmed publisher
  7. Mancek Keber M, Lainšček D, Bencina M, Chen J, Romih R, Hunter Z, et al. Extracellular vesicle-mediated transfer of constitutively active MyD88L265P engages MyD88wt and activates signaling. Blood. 2018;: pubmed publisher
    ..Results establish the mechanism of transmission of signaling complexes via EVs to propagate inflammation as a new mechanism of intercellular communication. ..
  8. Pirher N, Pohar J, Manček Keber M, Benčina M, Jerala R. Activation of cell membrane-localized Toll-like receptor 3 by siRNA. Immunol Lett. 2017;189:55-63 pubmed publisher
    ..These results contribute to understanding of the mechanism of side effects seen in the therapeutic application of naked, unmodified siRNA as a result of the activation of TLR3 localized at the plasma membrane. ..
  9. Smole A, Lainšček D, Bezeljak U, Horvat S, Jerala R. A Synthetic Mammalian Therapeutic Gene Circuit for Sensing and Suppressing Inflammation. Mol Ther. 2017;25:102-119 pubmed publisher
    ..The microencapsulated anti-inflammatory device significantly reduced the pathology in dextran sodium sulfate (DSS)-induced acute murine colitis, demonstrating a synthetic immunological approach for autonomous anti-inflammatory therapy. ..
  10. request reprint
    Aupič J, Lapenta F, StrmÅ¡ek Å, Jerala R. Towards designing new nano-scale protein architectures. Essays Biochem. 2016;60:315-324 pubmed
    ..This review offers an overview on rational and computational design approaches focusing on the main achievements of novel protein nanostructure design. ..
  11. request reprint
    Drobnak I, Ljubetic A, Gradisar H, Pisanski T, Jerala R. Designed Protein Origami. Adv Exp Med Biol. 2016;940:7-27 pubmed
    ..We envision that by expansion of the toolkit of building blocks and design strategies of the folding pathways protein origami technology will be able to construct diverse molecular machines. ..
  12. Božič Abram S, Aupič J, Dražić G, Gradišar H, Jerala R. Coiled-coil forming peptides for the induction of silver nanoparticles. Biochem Biophys Res Commun. 2016;472:566-71 pubmed publisher
    ..These results show that our designed peptides generate AgNPs with antibacterial activity at mild conditions and might be used for antibacterial coatings. ..
  13. Avbelj M, Panter G, Jerala R. The role of N-terminal segment and membrane association in MyD88-mediated signaling. Biochem Biophys Res Commun. 2018;495:878-883 pubmed publisher
    ..Moreover, anchoring of MyD88 to the cell membrane augments signaling supporting the importance of membrane localization in MyD88-mediated signaling. ..
  14. Pohar J, Lainšček D, Kunšek A, Cajnko M, Jerala R, Benčina M. Phosphodiester backbone of the CpG motif within immunostimulatory oligodeoxynucleotides augments activation of Toll-like receptor 9. Sci Rep. 2017;7:14598 pubmed publisher
    ..Our results highlight the functional significance of the phosphodiester linkage of a CpG dinucleotide for binding, which is important in designing improved immunostimulatory TLR9 agonists. ..
  15. Ljubetic A, Gradisar H, Jerala R. Advances in design of protein folds and assemblies. Curr Opin Chem Biol. 2017;40:65-71 pubmed publisher