Mojca Bencina

Summary

Affiliation: National Institute of Chemistry
Country: Slovenia

Publications

  1. ncbi request reprint A comparative genomic analysis of calcium and proton signaling/homeostasis in Aspergillus species
    Mojca Bencina
    Department of Biotechnology, National Institute of Chemistry, PO Box 660, Sl 1001 Ljublijana, Slovenia
    Fungal Genet Biol 46:S93-S104. 2009
  2. pmc The Aspergillus giganteus antifungal protein AFPNN5353 activates the cell wall integrity pathway and perturbs calcium homeostasis
    Ulrike Binder
    Biocenter, Division of Molecular Biology, Innsbruck Medical University, Fritz Pregl Strasse 3, Innsbruck, A 6020, Austria
    BMC Microbiol 11:209. 2011
  3. doi request reprint Noninvasive high-throughput single-cell analysis of HIV protease activity using ratiometric flow cytometry
    Rok Gaber
    Laboratory of Biotechnology, National Institute of Chemistry, Ljubljana 1000, Slovenia
    Sensors (Basel) 13:16330-46. 2013
  4. doi request reprint Illumination of the Spatial Order of Intracellular pH by Genetically Encoded pH-Sensitive Sensors
    Mojca Bencina
    Laboratory of Biotechnology, National Institute of Chemistry, 1000 Ljubljana, Slovenia
    Sensors (Basel) 13:16736-58. 2013
  5. pmc Mycoplasma synoviae induces upregulation of apoptotic genes, secretion of nitric oxide and appearance of an apoptotic phenotype in infected chicken chondrocytes
    Daliborka Dusanic
    University of Ljubljana, Department of Animal Science, Chair for Genetics, Animal Biotechnology and Immunology, Groblje 3, 1230 Domzale, Slovenia
    Vet Res 43:7. 2012
  6. pmc The role of UNC93B1 protein in surface localization of TLR3 receptor and in cell priming to nucleic acid agonists
    Jelka Pohar
    National Institute of Chemistry, Hajdrihova 19, Slovenia
    J Biol Chem 288:442-54. 2013
  7. doi request reprint Determination of the physiological 2:2 TLR5:flagellin activation stoichiometry revealed by the activity of a fusion receptor
    Karolina Ivičak-Kocjan
    Laboratory of Biotechnology, National Institute of Chemistry, 1000 Ljubljana, Slovenia
    Biochem Biophys Res Commun 435:40-5. 2013
  8. doi request reprint Highly active, citrate inhibition resistant form of Aspergillus niger 6-phosphofructo-1-kinase encoded by a modified pfkA gene
    Maja Capuder
    National Institute of Chemistry, Ljubljana, Slovenia
    J Biotechnol 144:51-7. 2009
  9. doi request reprint Enhancing itaconic acid production by Aspergillus terreus
    Gregor Tevz
    National Institute of Chemistry, Hajdrihova 19, 1000 Ljubljana, Slovenia
    Appl Microbiol Biotechnol 87:1657-64. 2010
  10. ncbi request reprint Monolithic bioreactors for macromolecules
    Mojca Bencina
    Laboratory of Biotechnology, National Institute of Chemistry, Ljubljana, Slovenia
    Methods Mol Biol 421:257-74. 2008

Collaborators

Detail Information

Publications26

  1. ncbi request reprint A comparative genomic analysis of calcium and proton signaling/homeostasis in Aspergillus species
    Mojca Bencina
    Department of Biotechnology, National Institute of Chemistry, PO Box 660, Sl 1001 Ljublijana, Slovenia
    Fungal Genet Biol 46:S93-S104. 2009
    ....
  2. pmc The Aspergillus giganteus antifungal protein AFPNN5353 activates the cell wall integrity pathway and perturbs calcium homeostasis
    Ulrike Binder
    Biocenter, Division of Molecular Biology, Innsbruck Medical University, Fritz Pregl Strasse 3, Innsbruck, A 6020, Austria
    BMC Microbiol 11:209. 2011
    ..The protein inhibits the germination and growth of filamentous ascomycetes, including important human and plant pathogens and the model organsims Aspergillus nidulans and Aspergillus niger...
  3. doi request reprint Noninvasive high-throughput single-cell analysis of HIV protease activity using ratiometric flow cytometry
    Rok Gaber
    Laboratory of Biotechnology, National Institute of Chemistry, Ljubljana 1000, Slovenia
    Sensors (Basel) 13:16330-46. 2013
    ..In addition, we have used a confocal microscopy sensitized emission FRET technique to evaluate the usefulness of the FRET-HIV sensor for spatiotemporal detection of intracellular HIV protease activity. ..
  4. doi request reprint Illumination of the Spatial Order of Intracellular pH by Genetically Encoded pH-Sensitive Sensors
    Mojca Bencina
    Laboratory of Biotechnology, National Institute of Chemistry, 1000 Ljubljana, Slovenia
    Sensors (Basel) 13:16736-58. 2013
    ..Finally, we illustrate some examples of the applications of pH sensors targeted at different subcellular compartments. ..
  5. pmc Mycoplasma synoviae induces upregulation of apoptotic genes, secretion of nitric oxide and appearance of an apoptotic phenotype in infected chicken chondrocytes
    Daliborka Dusanic
    University of Ljubljana, Department of Animal Science, Chair for Genetics, Animal Biotechnology and Immunology, Groblje 3, 1230 Domzale, Slovenia
    Vet Res 43:7. 2012
    ..Hence, the study gives new insights into the role of mycoplasma infection on chondrocyte biology and development of infectious arthritis in chickens and potentially in humans...
  6. pmc The role of UNC93B1 protein in surface localization of TLR3 receptor and in cell priming to nucleic acid agonists
    Jelka Pohar
    National Institute of Chemistry, Hajdrihova 19, Slovenia
    J Biol Chem 288:442-54. 2013
    ..Our findings identified TLR3 as the important regulator of UNC93B1 that in turn governs the responsiveness of all NAS TLRs...
  7. doi request reprint Determination of the physiological 2:2 TLR5:flagellin activation stoichiometry revealed by the activity of a fusion receptor
    Karolina Ivičak-Kocjan
    Laboratory of Biotechnology, National Institute of Chemistry, 1000 Ljubljana, Slovenia
    Biochem Biophys Res Commun 435:40-5. 2013
    ..These results, in addition to the combinations of inactive P736H mutation within the BB-loop of the TIR domain of TLR5 and SF-TLR5, further confirm the mechanism of TLR5 activation...
  8. doi request reprint Highly active, citrate inhibition resistant form of Aspergillus niger 6-phosphofructo-1-kinase encoded by a modified pfkA gene
    Maja Capuder
    National Institute of Chemistry, Ljubljana, Slovenia
    J Biotechnol 144:51-7. 2009
    ..An active shorter PFK1 fragment, resistant to citrate inhibition and activated to a higher level by fructose-2,6-bisphosphate with respect to the native enzyme was encoded directly from the modified gene...
  9. doi request reprint Enhancing itaconic acid production by Aspergillus terreus
    Gregor Tevz
    National Institute of Chemistry, Hajdrihova 19, 1000 Ljubljana, Slovenia
    Appl Microbiol Biotechnol 87:1657-64. 2010
    ..On the other hand, growth rate of all transformants remained suppressed which is due to the low initial pH value of the medium, one of the prerequisites for the accumulation of itaconic acid by A. terreus mycelium...
  10. ncbi request reprint Monolithic bioreactors for macromolecules
    Mojca Bencina
    Laboratory of Biotechnology, National Institute of Chemistry, Ljubljana, Slovenia
    Methods Mol Biol 421:257-74. 2008
    ..Immobilized efficiency is evaluated for different immobilization procedures in terms of biologic activity and long-term stability. Finally, their performance on real samples is demonstrated...
  11. ncbi request reprint Lipid composition of cAMP-dependent protein kinase mutants of Aspergillus niger
    Katarina Jernejc
    Laboratory of Biotechnology, National Institute of Chemistry, Hajdrihova 19, SI 1000 Ljubljana, Slovenia
    FEMS Microbiol Lett 225:291-7. 2003
    ..The results indicate the involvement of cAMP-mediated PKA activity in regulation of lipid biosynthesis as well as citric acid synthesis...
  12. ncbi request reprint Separation of pectin methylesterase isoenzymes from tomato fruits using short monolithic columns
    Irena Vovk
    Laboratory for Food Chemistry, National Institute of Chemistry, Hajdrihova 19, SI 1000 Ljubljana, Slovenia
    J Chromatogr A 1065:121-8. 2005
    ..The potential application of CIM disk short monolithic columns in the analysis and semi-preparative extraction and isolation of the PME isoenzyme is presented...
  13. doi request reprint MARCKS as a negative regulator of lipopolysaccharide signaling
    Mateja Mancek-Keber
    Department of Biotechnology, National Institute of Chemistry, Ljubljana 1000, Slovenia
    J Immunol 188:3893-902. 2012
    ..TLR4 signaling was enhanced by the ablation of MARCKS, which had no effect on stimulation by TLR2, TLR3, and TLR5 agonists. These findings demonstrate that MARCKS contributes to the negative regulation of the cellular response to LPS...
  14. doi request reprint Mechanism of endosomal TLR inhibition by antimalarial drugs and imidazoquinolines
    Alenka Kuznik
    Department of Biotechnology, National Institute of Chemistry, Ljubljana, Slovenia
    J Immunol 186:4794-804. 2011
    ..We conclude that the direct binding of inhibitors to nucleic acids mask their TLR-binding epitope and may explain the efficiency of those compounds in the treatment of autoimmune diseases...
  15. pmc NLRP3 inflammasome activation in macrophage cell lines by prion protein fibrils as the source of IL-1β and neuronal toxicity
    Iva Hafner-Bratkovic
    Department of Biotechnology, National Institute of Chemistry, Hajdrihova 19, 1000 Ljubljana, Slovenia
    Cell Mol Life Sci 69:4215-28. 2012
    ..Our results together with previous studies on IL-1R-deficient mice suggest the IL-1 signaling pathway as the perspective target for the therapy of prion disease...
  16. pmc Live-Cell imaging and measurement of intracellular pH in filamentous fungi using a genetically encoded ratiometric probe
    Tanja Bagar
    Department of Biotechnology, National Institute of Chemistry, Ljubljana, Slovenia
    Eukaryot Cell 8:703-12. 2009
    ..2 and 6.5. Overall, our study demonstrates that RaVC is an excellent probe for visualizing and quantifying intracellular pH in living fungal hyphae...
  17. ncbi request reprint Characterization of methacrylate monoliths for purification of DNA molecules
    Mojca Bencina
    Laboratory for Biotechnology and Industrial Mycology, National Institute of Chemistry, Hajdrihova 19, SI 1000 Ljubljana, Slovenia
    J Sep Sci 27:801-10. 2004
    ..Under optimal conditions recoveries exceeding 80% were obtained even for genomic DNA. Finally, the suitability of this approach is demonstrated by purification of a real-life sample...
  18. doi request reprint Expression of soluble versatile peroxidase of Bjerkandera adusta in Escherichia coli
    Martina Mohorcic
    Department of Biotechnology, National Institute of Chemistry, Hajdrihova 19, SI 1000 Ljubljana, Slovenia
    Bioresour Technol 100:851-8. 2009
    ..By exploiting an appropriate expression system and providing suitable culture conditions, the recombinant fungal peroxidases in soluble form can be produced in bacteria...
  19. doi request reprint Chimeric flagellin as the self-adjuvanting antigen for the activation of immune response against Helicobacter pylori
    Jerneja Mori
    Department of Biotechnology, National Institute of Chemistry, Ljubljana, Slovenia
    Vaccine 30:5856-63. 2012
    ..pylori or other flagellated bacteria that evade TLR5 recognition...
  20. doi request reprint A second binding site for double-stranded RNA in TLR3 and consequences for interferon activation
    Nina Pirher
    Department of Biotechnology, National Institute of Chemistry, Hajdrihova 19, 1000 Ljubljana, Slovenia
    Nat Struct Mol Biol 15:761-3. 2008
    ..We propose that there are different arrangements of TLR3 ectodomains along the double-stranded RNA that could modulate the strength of the interferon response...
  21. pmc Toll/interleukin-1 receptor domain dimers as the platform for activation and enhanced inhibition of Toll-like receptor signaling
    Ota Fekonja
    Laboratory of Biotechnology, National Institute of Chemistry, 1000 Ljubljana, Slovenia
    J Biol Chem 287:30993-1002. 2012
    ..We propose a molecular model of MyD88-mediated signaling based on the dimerization of TIR domains as the limiting step...
  22. doi request reprint Antiarrhythmic drug amiodarone displays antifungal activity, induces irregular calcium response and intracellular acidification of Aspergillus niger - amiodarone targets calcium and pH homeostasis of A. niger
    Tanja Bagar
    Department of Biotechnology, National Institute of Chemistry, Hajdrihova 19, SI 1000 Ljubljana, Slovenia
    Fungal Genet Biol 49:779-91. 2012
    ..A combination of amiodarone and amphotericin B is additive in terms of cell viability and cytosolic calcium influx. In contrast, a combination of azole drugs and amiodarone has a synergistic effect on the viability of fungi...
  23. ncbi request reprint Preparation and characterisation of ribonuclease monolithic bioreactor
    Mojca Bencina
    Laboratory of Biotechnology, National Institute of Chemistry, Hajdrihova 19, P O Box 600, SI 1001 Ljubljana, Slovenia
    J Chromatogr A 1144:135-42. 2007
    ..It was active for several weeks of operation and processed 300 column volumes of sample. Required residence time to eliminate RNA was estimated to be around 0.5 min enabling flow rates above 1 column volume per min...
  24. ncbi request reprint Cross-talk between cAMP and calcium signalling in Aspergillus niger
    Mojca Bencina
    Laboratory of Biotechnology and Industrial Mycology, National Institute of Chemistry, Hajdrihova 19, SI 1000 Ljubljana, Slovenia
    Mol Microbiol 56:268-81. 2005
    ..These results were consistent with PKA-dependent phosphorylation increasing [Ca2+]c to induce a polar to apolar shift in hyphal morphology...
  25. doi request reprint Tetracysteine-tagged prion protein allows discrimination between the native and converted forms
    Jernej Gaspersic
    Department of Biotechnology, National Institute of Chemistry, Ljubljana, Slovenia
    FEBS J 277:2038-50. 2010
    ....
  26. ncbi request reprint Cyclic AMP-dependent protein kinase is involved in morphogenesis of Aspergillus niger
    Mojca Saudohar
    National Institute of Chemistry, Department for Biotechnology and Industrial Mycology, Hajdrihova 19, SI 1000 Ljubljana, Slovenia
    Microbiology 148:2635-45. 2002
    ..These data suggest that cAMP-dependent protein phosphorylation in A. niger regulates growth polarity and formation of conidiospores...