A Berlec

Summary

Affiliation: Jozef Stefan Institute
Country: Slovenia

Publications

  1. ncbi request reprint Novel applications of recombinant lactic acid bacteria in therapy and in metabolic engineering
    Ales Berlec
    Department of Biotechnology, Jozef Stefan Institute, Jamova 39, SI 1000 Ljubljana, Slovenia
    Recent Pat Biotechnol 3:77-87. 2009
  2. doi request reprint Expression of a hepatitis A virus antigen in Lactococcus lactis and Escherichia coli and evaluation of its immunogenicity
    Ales Berlec
    Department of Biotechnology, Jozef Stefan Institute, Jamova 39, 1000 Ljubljana, Slovenia
    Appl Microbiol Biotechnol 97:4333-42. 2013
  3. doi request reprint Current state and recent advances in biopharmaceutical production in Escherichia coli, yeasts and mammalian cells
    Ales Berlec
    Department of Biotechnology, Jozef Stefan Institute, Jamova 39, Ljubljana, Slovenia
    J Ind Microbiol Biotechnol 40:257-74. 2013
  4. doi request reprint Novel techniques and findings in the study of plant microbiota: search for plant probiotics
    Ales Berlec
    Department of Biotechnology, Jozef Stefan Institute, Jamova 39, SI 1000 Ljubljana, Slovenia
    Plant Sci 193:96-102. 2012
  5. doi request reprint Generating a custom TA-cloning expression plasmid for Lactococcus lactis
    Ales Berlec
    Department of Biotechnology, Jozef Stefan Institute, Ljubljana, Slovenia
    Biotechniques 52:51-3. 2012
  6. pmc Identification of candidate carrier proteins for surface display on Lactococcus lactis by theoretical and experimental analyses of the surface proteome
    Ales Berlec
    Department of Biotechnology, Jozef Stefan Institute, Jamova 39, SI 1000 Ljubljana, Slovenia
    Appl Environ Microbiol 77:1292-300. 2011
  7. pmc Engineered lactic acid bacterium Lactococcus lactis capable of binding antibodies and tumor necrosis factor alpha
    Matjaz Ravnikar
    Department of Biotechnology, Jozef Stefan Institute, Jamova 39, SI 1000 Ljubljana, Slovenia
    Appl Environ Microbiol 76:6928-32. 2010
  8. doi request reprint Large increase in brazzein expression achieved by changing the plasmid /strain combination of the NICE system in Lactococcus lactis
    A Berlec
    Department of Biotechnology, Jozef Stefan Institute, Jamova 39, SI 1000 Ljubljana, Slovenia
    Lett Appl Microbiol 48:750-5. 2009
  9. doi request reprint Optimization of fermentation conditions for the expression of sweet-tasting protein brazzein in Lactococcus lactis
    A Berlec
    Department of Biotechnology, Jozef Stefan Institute, Ljubljana, Slovenia
    Lett Appl Microbiol 46:227-31. 2008
  10. ncbi request reprint Expression of the sweet-tasting plant protein brazzein in Escherichia coli and Lactococcus lactis: a path toward sweet lactic acid bacteria
    Ales Berlec
    Department of Biochemistry and Molecular Biology, Jozef Stefan Institute, Jamova 39, SI 1000, Ljubljana, Slovenia
    Appl Microbiol Biotechnol 73:158-65. 2006

Collaborators

Detail Information

Publications12

  1. ncbi request reprint Novel applications of recombinant lactic acid bacteria in therapy and in metabolic engineering
    Ales Berlec
    Department of Biotechnology, Jozef Stefan Institute, Jamova 39, SI 1000 Ljubljana, Slovenia
    Recent Pat Biotechnol 3:77-87. 2009
    ..Existing metabolic pathways have been modified to improve the properties of LAB as food fermentation starters, and heterologous metabolic enzymes have been introduced to LAB for the production of industrially relevant chemicals...
  2. doi request reprint Expression of a hepatitis A virus antigen in Lactococcus lactis and Escherichia coli and evaluation of its immunogenicity
    Ales Berlec
    Department of Biotechnology, Jozef Stefan Institute, Jamova 39, 1000 Ljubljana, Slovenia
    Appl Microbiol Biotechnol 97:4333-42. 2013
    ..Nevertheless, orally administered HAV antigen expressed in L. lactis elicited significant systemic humoral immune response showing the feasibility for development of effective HAV vaccine for mucosal delivery...
  3. doi request reprint Current state and recent advances in biopharmaceutical production in Escherichia coli, yeasts and mammalian cells
    Ales Berlec
    Department of Biotechnology, Jozef Stefan Institute, Jamova 39, Ljubljana, Slovenia
    J Ind Microbiol Biotechnol 40:257-74. 2013
    ..Systemic approaches, together with technological advances such as disposable bioreactors and microbioreactors, are expected to lead to increased quality and quantity of biopharmaceuticals, as well as to reduced product development times...
  4. doi request reprint Novel techniques and findings in the study of plant microbiota: search for plant probiotics
    Ales Berlec
    Department of Biotechnology, Jozef Stefan Institute, Jamova 39, SI 1000 Ljubljana, Slovenia
    Plant Sci 193:96-102. 2012
    ..Administration of microbial mixtures of whole communities, rather than individual species, is highlighted and should be considered in future agricultural applications...
  5. doi request reprint Generating a custom TA-cloning expression plasmid for Lactococcus lactis
    Ales Berlec
    Department of Biotechnology, Jozef Stefan Institute, Ljubljana, Slovenia
    Biotechniques 52:51-3. 2012
    ..Cloning using pNZ-T yielded a high proportion of insert-containing plasmids on transformation. Although demonstrated with L. lactis, the technique presented here is organism-independent and can be implemented in other plasmids...
  6. pmc Identification of candidate carrier proteins for surface display on Lactococcus lactis by theoretical and experimental analyses of the surface proteome
    Ales Berlec
    Department of Biotechnology, Jozef Stefan Institute, Jamova 39, SI 1000 Ljubljana, Slovenia
    Appl Environ Microbiol 77:1292-300. 2011
    ..The B domain was displayed on the surface with BmpA as a carrier. The advantage of covalent BmpA binding was demonstrated. BmpA was thus shown to be a suitable candidate for a carrier protein in lactococcal surface display...
  7. pmc Engineered lactic acid bacterium Lactococcus lactis capable of binding antibodies and tumor necrosis factor alpha
    Matjaz Ravnikar
    Department of Biotechnology, Jozef Stefan Institute, Jamova 39, SI 1000 Ljubljana, Slovenia
    Appl Environ Microbiol 76:6928-32. 2010
    ..A tumor necrosis factor alpha (TNF-α)-binding affibody was also displayed and bound TNF-α, which could be useful in the treatment of inflammatory bowel disease...
  8. doi request reprint Large increase in brazzein expression achieved by changing the plasmid /strain combination of the NICE system in Lactococcus lactis
    A Berlec
    Department of Biotechnology, Jozef Stefan Institute, Jamova 39, SI 1000 Ljubljana, Slovenia
    Lett Appl Microbiol 48:750-5. 2009
    ..To evaluate brazzein production in Lactococcus lactis using the nisin-controlled expression (NICE) system. The approach is through analysis of different plasmid/strain combinations...
  9. doi request reprint Optimization of fermentation conditions for the expression of sweet-tasting protein brazzein in Lactococcus lactis
    A Berlec
    Department of Biotechnology, Jozef Stefan Institute, Ljubljana, Slovenia
    Lett Appl Microbiol 46:227-31. 2008
    ..To improve the production of sweet-tasting protein brazzein in Lactococcus lactis using controlled fermentation conditions...
  10. ncbi request reprint Expression of the sweet-tasting plant protein brazzein in Escherichia coli and Lactococcus lactis: a path toward sweet lactic acid bacteria
    Ales Berlec
    Department of Biochemistry and Molecular Biology, Jozef Stefan Institute, Jamova 39, SI 1000, Ljubljana, Slovenia
    Appl Microbiol Biotechnol 73:158-65. 2006
    ..Secretion of brazzein into the medium has not led to significant yield increase. Surprisingly, optimizing the codon usage for Lactococcus lactis led to a decrease in the yield of brazzein...
  11. ncbi request reprint Lactic acid bacteria as oral delivery systems for biomolecules
    A Berlec
    Department of Biotechnology, Jozef Stefan Institute, Ljubljana, Slovenia
    Pharmazie 67:891-8. 2012
    ..The delivery of DNA by LAB was demonstrated and offers exciting opportunities, especially as a vaccine. New discoveries may eventually lead to the transition of LAB as delivery systems in clinical practice...
  12. ncbi request reprint Calcipotriol affects keratinocyte proliferation by decreasing expression of early growth response-1 and polo-like kinase-2
    Jernej Kristl
    Department of Pharmaceutical Biology, Faculty of Pharmacy, University of Ljubljana, Askerceva 7, 1000, Ljubljana, Slovenia
    Pharm Res 25:521-9. 2008
    ..In the present study we investigated the effects of calcipotriol on gene expression in human keratinocytes in terms of mechanism of how calcipotriol decreases proliferation...