Lokendra Singh

Summary

Publications

  1. doi request reprint Multiplex detection of protein toxins using MALDI-TOF-TOF tandem mass spectrometry: application in unambiguous toxin detection from bioaerosol
    Syed Imteyaz Alam
    Biotechnology Division, Defence Research and Development Establishment, Gwalior, India
    Anal Chem 84:10500-7. 2012
  2. doi request reprint Characterization of an environmental strain of Bacillus thuringiensis from a hot spring in Western Himalayas
    Syed Imteyaz Alam
    Biotechnology Division, Defence Research and Development Establishment, Gwalior, India
    Curr Microbiol 62:547-56. 2011
  3. doi request reprint Comparative genomic analysis of a neurotoxigenic Clostridium species using partial genome sequence: Phylogenetic analysis of a few conserved proteins involved in cellular processes and metabolism
    Syed Imteyaz Alam
    Biotechnology Division, Defence Research and Development Establishment, Gwalior 474002, India
    Anaerobe 16:147-54. 2010
  4. ncbi request reprint Deletion mutant comprising 198 residues of BoNT/A toxin receptor binding domain retained gt1b binding property but failed to induce protective antibody response in a mouse model
    Manglesh Kumar Singh
    Biotechnology Division, Protective Device Division, Defence Research and Development Establishment, Gwalior 474002, India
    Protein Pept Lett 19:530-7. 2012
  5. pmc Comparative proteomic analysis of extracellular proteins of Clostridium perfringens type A and type C strains
    Nabonita Sengupta
    Biotechnology Division, Defence Research and Development Establishment, Gwalior 474002, India
    Infect Immun 78:3957-68. 2010
  6. ncbi request reprint Purification and characterization of an extracellular protease produced by psychrotolerant Clostridium sp. LP3 from lake sediment of Leh, India
    Syed Imteyaz Alam
    Biotechnology Division, Defence Research and Development Establishment, Gwalior, India
    Can J Microbiol 52:1238-46. 2006
  7. pmc Immunization against Clostridium perfringens cells elicits protection against Clostridium tetani in mouse model: identification of cross-reactive proteins using proteomic methodologies
    Syed Imteyaz Alam
    Biotechnology Division, Defence Research and Development Establishment, Gwalior, India
    BMC Microbiol 8:194. 2008
  8. doi request reprint Bacterial diversity of soil samples from the western Himalayas, India
    Pooja Gangwar
    Biotechnology Division, Defence Research and Development Establishment, Gwalior, India
    Can J Microbiol 55:564-77. 2009
  9. ncbi request reprint Sequencing and phylogenetic analysis of neurotoxin gene from an environmental isolate of Clostridium sp.: comparison with other clostridial neurotoxins
    Aparna Dixit
    Biotechnology Division, Defence Research and Development Establishment, 474002 Gwalior, India
    Arch Toxicol 80:399-404. 2006
  10. ncbi request reprint Isolation and characterization of heat resistant enterotoxigenic Staphylococcus aureus from a food poisoning outbreak in Indian subcontinent
    Vijay Nema
    Biotechnology Division, Defence Research and Development Establishment, Jhansi Road, Gwalior 474 002, India
    Int J Food Microbiol 117:29-35. 2007

Collaborators

Detail Information

Publications26

  1. doi request reprint Multiplex detection of protein toxins using MALDI-TOF-TOF tandem mass spectrometry: application in unambiguous toxin detection from bioaerosol
    Syed Imteyaz Alam
    Biotechnology Division, Defence Research and Development Establishment, Gwalior, India
    Anal Chem 84:10500-7. 2012
    ..ETX prototoxin could be detected from aerosol at 0.2 ppb concentration in aerosol...
  2. doi request reprint Characterization of an environmental strain of Bacillus thuringiensis from a hot spring in Western Himalayas
    Syed Imteyaz Alam
    Biotechnology Division, Defence Research and Development Establishment, Gwalior, India
    Curr Microbiol 62:547-56. 2011
    ..thuringiensis. This strategy can be used to assign strains of B. cereus group to their respective species...
  3. doi request reprint Comparative genomic analysis of a neurotoxigenic Clostridium species using partial genome sequence: Phylogenetic analysis of a few conserved proteins involved in cellular processes and metabolism
    Syed Imteyaz Alam
    Biotechnology Division, Defence Research and Development Establishment, Gwalior 474002, India
    Anaerobe 16:147-54. 2010
    ..There is an overall conservation of protein sequences among the two strains of C. tetani and. Selected ORFs involved in cellular processes and metabolism were subjected to phylogenetic analysis...
  4. ncbi request reprint Deletion mutant comprising 198 residues of BoNT/A toxin receptor binding domain retained gt1b binding property but failed to induce protective antibody response in a mouse model
    Manglesh Kumar Singh
    Biotechnology Division, Protective Device Division, Defence Research and Development Establishment, Gwalior 474002, India
    Protein Pept Lett 19:530-7. 2012
    ..Antibody titre in serum was determined by enzyme linked immunosorbent assay and specific binding to rBoNT/A-HCC was demonstrated by surface plasmon resonance (SPR), with a dissociation constant of 0.8 pM...
  5. pmc Comparative proteomic analysis of extracellular proteins of Clostridium perfringens type A and type C strains
    Nabonita Sengupta
    Biotechnology Division, Defence Research and Development Establishment, Gwalior 474002, India
    Infect Immun 78:3957-68. 2010
    ..There were significant increases in the levels of gamma interferon (IFN-gamma) and interleukin 2 (IL-2), suggesting a Th1 type immune response...
  6. ncbi request reprint Purification and characterization of an extracellular protease produced by psychrotolerant Clostridium sp. LP3 from lake sediment of Leh, India
    Syed Imteyaz Alam
    Biotechnology Division, Defence Research and Development Establishment, Gwalior, India
    Can J Microbiol 52:1238-46. 2006
    ..It was thermolabile and resistant to sodium dodecyl sulfate. Enzyme kinetics showed a tendency to increase Km with an increase in temperature for casein substrate...
  7. pmc Immunization against Clostridium perfringens cells elicits protection against Clostridium tetani in mouse model: identification of cross-reactive proteins using proteomic methodologies
    Syed Imteyaz Alam
    Biotechnology Division, Defence Research and Development Establishment, Gwalior, India
    BMC Microbiol 8:194. 2008
    ..We tested a likelihood of extensive sharing of common epitopes between homologous proteins of these two medically important pathogens and the possibility of cross-protection using active immunization...
  8. doi request reprint Bacterial diversity of soil samples from the western Himalayas, India
    Pooja Gangwar
    Biotechnology Division, Defence Research and Development Establishment, Gwalior, India
    Can J Microbiol 55:564-77. 2009
    ..The bacterial diversity exhibited an altitude gradient with a gradual decline in the number of genera with increase in altitude. The isolates exhibited close phylogenetic affinities to bacteria from other cold habitats...
  9. ncbi request reprint Sequencing and phylogenetic analysis of neurotoxin gene from an environmental isolate of Clostridium sp.: comparison with other clostridial neurotoxins
    Aparna Dixit
    Biotechnology Division, Defence Research and Development Establishment, 474002 Gwalior, India
    Arch Toxicol 80:399-404. 2006
    ..The C-terminal region responsible for binding to receptor was conserved, showing no changes in the amino acid sequence...
  10. ncbi request reprint Isolation and characterization of heat resistant enterotoxigenic Staphylococcus aureus from a food poisoning outbreak in Indian subcontinent
    Vijay Nema
    Biotechnology Division, Defence Research and Development Establishment, Jhansi Road, Gwalior 474 002, India
    Int J Food Microbiol 117:29-35. 2007
    ..Isolates obtained in the current outbreak need to be further studied to find out the impact on food safety guidelines with respect to thermal processing...
  11. ncbi request reprint Characterization of Clostridium sp. RKD producing botulinum-like neurotoxin
    Aparna Dixit
    Biotechnology Division, Defence Research and Development Establishment, Jhansi Road, Gwalior 474002, India
    Syst Appl Microbiol 28:405-14. 2005
    ..Sequence of nearly 700 bp amplified using primers specific for botulinum neurotoxin type B gene, did not show any significant match in the database when subjected to BLAST search...
  12. ncbi request reprint Development of an immunodetection test for a botulinum-like neurotoxin produced by Clostridium sp. RKD
    Aparna Dixit
    Biotechnology Division, Defence Research and Development Establishment, Gwalior, India
    Indian J Med Res 124:355-62. 2006
    ..The aim of the present investigation was to develop a laboratory based detection assay as an alternative to the mouse bioassay without compromising sensitivity and specificity...
  13. doi request reprint Diversity and antibiotic susceptibility pattern of cultivable anaerobic bacteria from soil and sewage samples of India
    Nabonita Sengupta
    Biotechnology Division, Defence Research and Development Establishment, Gwalior 474002, India
    Infect Genet Evol 11:64-77. 2011
    ..Understanding the diversity of the anaerobic bacteria from soil and sewage with respect to animal health and spread of zoonotic pathogen infections is crucial for improvements in animal and human health...
  14. ncbi request reprint Clostridium schirmacherense sp. nov., an obligately anaerobic, proteolytic, psychrophilic bacterium isolated from lake sediment of Schirmacher Oasis, Antarctica
    Syed Imteyaz Alam
    Biotechnology Division, Defence Research and Development Establishment, Gwalior 474002, India
    Int J Syst Evol Microbiol 56:715-20. 2006
    ..nov. is proposed. The type strain is AP15T (=DSM 17394T = JCM 13289T)...
  15. ncbi request reprint Characterization of LC-HCC fusion protein of botulinum neurotoxin type A
    Manglesh Kumar Singh
    Biotechnology Division, Defence Research and Development Establishment, Gwalior, India
    Protein Pept Lett 18:295-304. 2011
    ..Biologically active recombinant fusion protein was also evaluated for its immunological potential...
  16. pmc Construction of a single-chain variable-fragment antibody against the superantigen Staphylococcal enterotoxin B
    Pawan Kumar Singh
    Biotechnology Division, Defence Research and Development Establishment, Jhansi Road, Gwalior 474002, India
    Appl Environ Microbiol 76:8184-91. 2010
    ..16 nM as determined by surface plasmon resonance (SPR). These results demonstrate that the recombinant antibody constructed by immortalizing the antibody genes from a hybridoma clone is useful for immunodetection of SEB...
  17. pmc Differential proteomic analysis of Clostridium perfringens ATCC13124; identification of dominant, surface and structure associated proteins
    Syed Imteyaz Alam
    Biotechnology Division, Defence Research and Development Establishment, Gwalior 474002, India
    BMC Microbiol 9:162. 2009
    ..Many of the virulence determinants and putative vaccine candidates for bacterial pathogens are known to be surface localized...
  18. pmc Highly Expressed Recombinant SEB for Antibody Production and Development of Immunodetection System
    Ranu Agrawal
    Defence Research and Development Establishment, Gwalior, Madhya Pradesh India
    Indian J Microbiol 52:191-6. 2012
    ..25 to 0.49 ng/ml or g of food. The detection system developed in the present study is at least as specific and sensitive as other commercially available kits which use monoclonal antibodies...
  19. pmc Development of ELISA based detection system for lethal toxin of Clostridium sordellii
    Preetika Arya
    Biotechnology Division, Defence Research and Development Establishment, Gwalior, India
    Indian J Med Res 137:1180-7. 2013
    ..Lethal toxin has gained more importance due its high toxicity. The present study was carried out to develop a sandwich ELISA for detection of lethal toxin of C. sordellii...
  20. ncbi request reprint Antibodies against recombinant catalytic domain of lethal toxin of Clostridium sordellii neutralize lethal toxin toxicity in HeLa cells
    Preetika Arya
    Biotechnology Division, Defence Research and Development Establishment, Gwalior 474002, M P, India
    Protein Pept Lett 20:205-12. 2013
    ..difficile native toxins in HeLa cells. Mice and rabbit anti-rTcsL sera effectively neutralized the cytotoxicity of rTcsL and C. sordellii native toxin but it did not neutralize the cytotoxicity of C. difficile toxin in HeLa cells...
  21. doi request reprint Biomethanation under psychrophilic conditions
    Ram Kumar Dhaked
    Biotechnology Division, Defence Research and Development Establishment, Jhansi Road, Gwalior 474002, India
    Waste Manag 30:2490-6. 2010
    ..Biomethanation at psychrophilic temperatures and the contribution made to night-soil degradation in the Himalayas are reviewed in this article...
  22. ncbi request reprint Effect of adjuvants on antibody titer of synthetic recombinant light chain of botulinum neurotoxin type B and its diagnostic potential for botulism
    Swati Jain
    Biotechnology Division, Defence Research and Development Establishment, Jhansi Road, Gwalior 474 002, Madhya Pradesh, India
    J Microbiol Biotechnol 21:719-27. 2011
    ..These developed high-titer antibodies may prove useful for the detection of botulinum neurotoxins in food and clinical samples...
  23. pmc Metabolic Characterization of cold active Pseudomonas, Arthrobacter, Bacillus, and Flavobacterium spp. from Western Himalayas
    Pooja Gangwar
    Biotechnology Division, Defence Research and Development Establishment, Jhansi Road, Gwalior, 474002 India
    Indian J Microbiol 51:70-5. 2011
    ..ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s12088-011-0092-7) contains supplementary material, which is available to authorized users...
  24. ncbi request reprint Amperometric immunosensor for the detection of Vibrio cholerae O1 using disposable screen-printed electrodes
    Vepa K Rao
    Defence Research and Development Establishment, Jhansi Road, Gwalior, India
    Anal Sci 22:1207-11. 2006
    ..The minimum detection limit of the amperometric immunosensor for V. cholerae was found to be 10(5) cells/ml in 55 min, while ELISA detected 10(6) cells/ml in 4 h...
  25. ncbi request reprint Surveillance methodology for Vibrio cholerae in environmental samples
    Akhilesh K Tamrakar
    Biotechnology Division, Defence Research and Development Establishment, Gwalior, India
    Int J Environ Health Res 16:305-12. 2006
    ..cholerae from the environmental samples. This study also showed that most of the environmental isolates are non-O1/non-O139 and the chances of presence of toxigenic V. cholerae are very rare in the environment...
  26. ncbi request reprint Effect of propionate toxicity on methanogenesis of night soil at phychrophilic temperature
    Ram Kumar Dhaked
    Defence Research and Development Establishment, Jhansi Road, Gwalior 474002, India
    Bioresour Technol 87:299-303. 2003
    ..Total volatile fatty acids increased with the increase in propionate concentration and particularly accumulation of propionate was observed. The results were also compared with the 30 degrees C fermentation...