Affiliation: Temasek Life Sciences Laboratory
Hu F, Lai E, Okamura K. A signaling-induced switch in dicer localization and function. Dev Cell. 2014;31:523-4 pubmed publisher
..This regulatory strategy, conserved in mammals, allows dynamic control of microRNA function required for Caenorhabditis elegans germline development and oogenesis. ..
Okamura K, Ladewig E, Zhou L, Lai E. Functional small RNAs are generated from select miRNA hairpin loops in flies and mammals. Genes Dev. 2013;27:778-92 pubmed publisher
..Altogether, we reveal a conserved mechanism that yields functional RNAs from miRNA loop regions, broadening the repertoire of Argonaute-dependent regulatory RNAs and providing evidence for functionality of endogenous ssRNA species...
Chak L, Mohammed J, Lai E, Tucker Kellogg G, Okamura K. A deeply conserved, noncanonical miRNA hosted by ribosomal DNA. RNA. 2015;21:375-84 pubmed publisher
..Our results suggest that biologically active miRNA genes may remain unidentified even in well-studied organisms. ..
Lim M, Okamura K. Switches in Dicer Activity During Oogenesis and Early Development. Results Probl Cell Differ. 2017;63:325-351 pubmed publisher
..Furthermore, we will discuss potential roles of Dicer in transgenerational inheritance of small RNA-mediated gene regulation. ..
Goh E, Okamura K. Gateway to Understanding Argonaute Loading of Single-Stranded RNAs: Preparation of Deep Sequencing Libraries with In Vitro Loading Samples. Methods Mol Biol. 2018;1680:41-63 pubmed publisher
..The method would aid in studying the mechanism behind the selective loading of ssRNAs to AGOs and may potentially be applied to study interactions between RNA and other RNA-binding proteins. ..
Lim M, Ng A, Chou Y, Lim T, Simcox A, Tucker Kellogg G, et al
. The Drosophila Dicer-1 Partner Loquacious Enhances miRNA Processing from Hairpins with Unstable Structures at the Dicing Site. Cell Rep. 2016;15:1795-808 pubmed publisher
..Finally, we found that evolutionarily young miRNA genes tended to be Loqs dependent. We propose that Loqs may have roles in assisting the de novo emergence of miRNA genes by facilitating dicing of suboptimal hairpin substrates. ..