Huck Hui Ng

Summary

Country: Singapore

Publications

  1. ncbi request reprint The Oct4 and Nanog transcription network regulates pluripotency in mouse embryonic stem cells
    Yuin Han Loh
    Gene Regulation Laboratory, Genome Institute of Singapore, Singapore 138672
    Nat Genet 38:431-40. 2006
  2. doi request reprint Transcriptional regulation in embryonic stem cells
    Jian Chien Dominic Heng
    NUS Graduate School for Integrative Sciences and Engineering, Singapore
    Adv Exp Med Biol 695:76-91. 2010
  3. pmc Transcriptomic analysis of pluripotent stem cells: insights into health and disease
    Jia Chi Yeo
    Gene Regulation Laboratory, Genome Institute of Singapore, 60 Biopolis Street, Genome, Singapore 138672
    Genome Med 3:68. 2011
  4. doi request reprint The transcriptional and signalling networks of pluripotency
    Huck Hui Ng
    Gene Regulation Laboratory, Genome Institute of Singapore, Singapore 138672, Singapore
    Nat Cell Biol 13:490-6. 2011
  5. ncbi request reprint Transcriptional regulation of nanog by OCT4 and SOX2
    David J Rodda
    Stem Cell and Developmental Biology and Gene Regulation Laboratory, Genome Institute of Singapore, Singapore 138672
    J Biol Chem 280:24731-7. 2005
  6. pmc Eset partners with Oct4 to restrict extraembryonic trophoblast lineage potential in embryonic stem cells
    Ping Yuan
    Gene Regulation Laboratory, Genome Institute of Singapore, Singapore 138672
    Genes Dev 23:2507-20. 2009
  7. ncbi request reprint Sall4 interacts with Nanog and co-occupies Nanog genomic sites in embryonic stem cells
    Qiang Wu
    Gene Regulation Laboratory, Genome Institute of Singapore, Singapore
    J Biol Chem 281:24090-4. 2006
  8. doi request reprint Transposable elements have rewired the core regulatory network of human embryonic stem cells
    Galih Kunarso
    Computational and Mathematical Biology, Genome Institute of Singapore, Singapore, Singapore
    Nat Genet 42:631-4. 2010
  9. pmc Tbx3 improves the germ-line competency of induced pluripotent stem cells
    Jianyong Han
    Stem Cell and Developmental Biology, Genome Institute of Singapore, 138672, Singapore
    Nature 463:1096-100. 2010
  10. pmc Jmjd1a and Jmjd2c histone H3 Lys 9 demethylases regulate self-renewal in embryonic stem cells
    Yuin Han Loh
    Gene Regulation Laboratory, Genome Institute of Singapore, Singapore 138672
    Genes Dev 21:2545-57. 2007

Collaborators

Detail Information

Publications47

  1. ncbi request reprint The Oct4 and Nanog transcription network regulates pluripotency in mouse embryonic stem cells
    Yuin Han Loh
    Gene Regulation Laboratory, Genome Institute of Singapore, Singapore 138672
    Nat Genet 38:431-40. 2006
    ..The emerging picture is one in which Oct4 and Nanog control a cascade of pathways that are intricately connected to govern pluripotency, self-renewal, genome surveillance and cell fate determination...
  2. doi request reprint Transcriptional regulation in embryonic stem cells
    Jian Chien Dominic Heng
    NUS Graduate School for Integrative Sciences and Engineering, Singapore
    Adv Exp Med Biol 695:76-91. 2010
    ..This chapter provides an overview of the significance of transcriptional regulation in ESC and traces the recent advances made in dissecting the ESC transcriptional regulatory network...
  3. pmc Transcriptomic analysis of pluripotent stem cells: insights into health and disease
    Jia Chi Yeo
    Gene Regulation Laboratory, Genome Institute of Singapore, 60 Biopolis Street, Genome, Singapore 138672
    Genome Med 3:68. 2011
    ..Finally, we address the importance of these advances for developing new therapeutics, and the future challenges facing the clinical application of ESCs and iPSCs...
  4. doi request reprint The transcriptional and signalling networks of pluripotency
    Huck Hui Ng
    Gene Regulation Laboratory, Genome Institute of Singapore, Singapore 138672, Singapore
    Nat Cell Biol 13:490-6. 2011
    ..Here we review the recent advances in studying the transcriptional regulatory networks that define pluripotency, and elaborate on how manipulation of signalling pathways can modulate pluripotent states to varying degrees...
  5. ncbi request reprint Transcriptional regulation of nanog by OCT4 and SOX2
    David J Rodda
    Stem Cell and Developmental Biology and Gene Regulation Laboratory, Genome Institute of Singapore, Singapore 138672
    J Biol Chem 280:24731-7. 2005
    ..These studies extend the understanding of the pluripotent genetic regulatory network within which the Sox2-Oct4 complex are at the top of the regulatory hierarchy...
  6. pmc Eset partners with Oct4 to restrict extraembryonic trophoblast lineage potential in embryonic stem cells
    Ping Yuan
    Gene Regulation Laboratory, Genome Institute of Singapore, Singapore 138672
    Genes Dev 23:2507-20. 2009
    ..Our results suggest that Eset restricts the extraembryonic trophoblast lineage potential of pluripotent cells and links an epigenetic regulator to key cell fate decision through a pluripotency factor...
  7. ncbi request reprint Sall4 interacts with Nanog and co-occupies Nanog genomic sites in embryonic stem cells
    Qiang Wu
    Gene Regulation Laboratory, Genome Institute of Singapore, Singapore
    J Biol Chem 281:24090-4. 2006
    ..We suggest that Sall4 and Nanog form a regulatory circuit similar to that of Oct4 and Sox2. This study highlights the extensive regulatory loops connecting genes, which encode for key transcription factors in ES cells...
  8. doi request reprint Transposable elements have rewired the core regulatory network of human embryonic stem cells
    Galih Kunarso
    Computational and Mathematical Biology, Genome Institute of Singapore, Singapore, Singapore
    Nat Genet 42:631-4. 2010
    ..These data indicate that species-specific transposable elements have substantially altered the transcriptional circuitry of pluripotent stem cells...
  9. pmc Tbx3 improves the germ-line competency of induced pluripotent stem cells
    Jianyong Han
    Stem Cell and Developmental Biology, Genome Institute of Singapore, 138672, Singapore
    Nature 463:1096-100. 2010
    ..This study underscores the intrinsic qualitative differences between iPS cells generated by different methods, and highlights the need to rigorously characterize iPS cells beyond in vitro studies...
  10. pmc Jmjd1a and Jmjd2c histone H3 Lys 9 demethylases regulate self-renewal in embryonic stem cells
    Yuin Han Loh
    Gene Regulation Laboratory, Genome Institute of Singapore, Singapore 138672
    Genes Dev 21:2545-57. 2007
    ..Our results connect the ES cell transcription circuitry to chromatin modulation through H3K9 demethylation in pluripotent cells...
  11. ncbi request reprint A global map of p53 transcription-factor binding sites in the human genome
    Chia Lin Wei
    Genome Institute of Singapore, Singapore 138672
    Cell 124:207-19. 2006
    ....
  12. doi request reprint The nuclear receptor Nr5a2 can replace Oct4 in the reprogramming of murine somatic cells to pluripotent cells
    Jian Chien Dominic Heng
    Gene Regulation Laboratory, Genome Institute of Singapore, Singapore 138672, Singapore
    Cell Stem Cell 6:167-74. 2010
    ..We also show that Nr5a2 works in part through activating Nanog. Together, we show that unrelated transcription factors can replace Oct4 and uncovers an exogenous Oct4-free reprogramming code...
  13. doi request reprint A genome-wide RNAi screen reveals determinants of human embryonic stem cell identity
    Na Yu Chia
    Gene Regulation Laboratory, Genome Institute of Singapore, 60 Biopolis Street, Singapore 138672
    Nature 468:316-20. 2010
    ..Altogether, our study uncovers a wealth of novel hESC regulators wherein PRDM14 exemplifies a key transcription factor required for the maintenance of hESC identity and the reacquisition of pluripotency in human somatic cells...
  14. pmc Zic3 is required for maintenance of pluripotency in embryonic stem cells
    Linda Shushan Lim
    Stem Cell and Developmental Biology Group, Gene Regulation Laboratory, Genome Institute of Singapore, Singapore 138672
    Mol Biol Cell 18:1348-58. 2007
    ....
  15. doi request reprint A core Klf circuitry regulates self-renewal of embryonic stem cells
    Jianming Jiang
    Gene Regulation Laboratory, Genome Institute of Singapore, 60 Biopolis Street, 02 01, Genome Building, Singapore 138672
    Nat Cell Biol 10:353-60. 2008
    ..Taken together, our study provides new insight into how the core Klf circuitry integrates into the Nanog transcriptional network to specify gene expression that is unique to ES cells...
  16. ncbi request reprint MicroRNA-134 modulates the differentiation of mouse embryonic stem cells, where it causes post-transcriptional attenuation of Nanog and LRH1
    Yvonne M S Tay
    Stem Cell and Developmental Biology, Genome Institute of Singapore, 02 01 Genome, Singapore 138672
    Stem Cells 26:17-29. 2008
    ....
  17. ncbi request reprint Sall4 modulates embryonic stem cell pluripotency and early embryonic development by the transcriptional regulation of Pou5f1
    Jinqiu Zhang
    Stem Cell and Developmental Biology, Genome Institute of Singapore, 60 Biopolis Street, Singapore 138672
    Nat Cell Biol 8:1114-23. 2006
    ..The data also indicates that Sall4 is important for early embryonic cell-fate decisions...
  18. doi request reprint Reprogramming of fibroblasts into induced pluripotent stem cells with orphan nuclear receptor Esrrb
    Bo Feng
    Gene Regulation Laboratory, Genome Institute of Singapore, 60 Biopolis Street, 02 01, Genome Building, Singapore 138672
    Nat Cell Biol 11:197-203. 2009
    ..Our findings also indicate that it is possible to reprogram MEFs without exogenous Klf transcription factors and link a nuclear receptor to somatic cell reprogramming...
  19. ncbi request reprint In vivo epigenomic profiling of germ cells reveals germ cell molecular signatures
    Jia Hui Ng
    Gene Regulation Laboratory, Genome Institute of Singapore, Singapore 138672
    Dev Cell 24:324-33. 2013
    ..Together, our study provides genome-wide histone modification maps of in vivo germ cells and reveals the molecular chromatin signatures of germ cells...
  20. doi request reprint Computer and statistical analysis of transcription factor binding and chromatin modifications by ChIP-seq data in embryonic stem cell
    Yuriy Orlov
    Genome Institute of Singapore, Singapore 138672, Singapore
    J Integr Bioinform 9:211. 2012
    ..The data provide new insights into the function of chromatin organization and regulation in stem cells...
  21. doi request reprint A PRC2-dependent repressive role of PRDM14 in human embryonic stem cells and induced pluripotent stem cell reprogramming
    Yun Shen Chan
    Gene Regulation Laboratory, Genome Institute of Singapore, Singapore, Singapore
    Stem Cells 31:682-92. 2013
    ..In summary, our study reveals a repressive role of PRDM14 in the maintenance and induction of pluripotency and identifies PRDM14 as a new regulator of PRC2...
  22. doi request reprint Genome-wide kinase-chromatin interactions reveal the regulatory network of ERK signaling in human embryonic stem cells
    Jonathan Goke
    Gene Regulation Laboratory, Genome Institute of Singapore, Singapore 138672, Singapore
    Mol Cell 50:844-55. 2013
    ..In summary, we propose a model wherein extracellular-signaling-stimulated proliferation and intrinsic repression of differentiation are integrated to maintain the identity of hESCs. ..
  23. ncbi request reprint Integration of external signaling pathways with the core transcriptional network in embryonic stem cells
    Xi Chen
    Gene Regulation Laboratory, Genome Institute of Singapore, Singapore 138672
    Cell 133:1106-17. 2008
    ..Collectively, the comprehensive mapping of TF-binding sites identifies important features of the transcriptional regulatory networks that define ES-cell identity...
  24. ncbi request reprint The retrovirus HERVH is a long noncoding RNA required for human embryonic stem cell identity
    Xinyi Lu
    Gene Regulation Laboratory, Genome Institute of Singapore, Singapore
    Nat Struct Mol Biol 21:423-5. 2014
    ..Furthermore, HERVH is associated with OCT4, coactivators and Mediator subunits. Together, these results uncover a new role of species-specific transposable elements in hESCs. ..
  25. ncbi request reprint p53-regulated transcriptional program associated with genotoxic stress-induced apoptosis
    Patricia S Kho
    Laboratory of Molecular Pharmacology, and Cell and Medical Biology, Genome Institute of Singapore, Genome Building 02 01, 60 Biopolis Street, Singapore 138672
    J Biol Chem 279:21183-92. 2004
    ..Our data support the divergent functions of p53 in regulating gene expression that play both synergistic and pleiotropic roles in p53-associated apoptosis...
  26. pmc BLIMP1 regulates cell growth through repression of p53 transcription
    Junli Yan
    Gene Expression Laboratory, Genome Institute of Singapore, Singapore
    Proc Natl Acad Sci U S A 104:1841-6. 2007
    ..Hence, suppression of p53 transcription is a crucial function of endogenous BLIMP1 and is essential for normal cell growth...
  27. doi request reprint Uniform, optimal signal processing of mapped deep-sequencing data
    Vibhor Kumar
    Computational and Systems Biology, Genome Institute of Singapore, Singapore
    Nat Biotechnol 31:615-22. 2013
    ..The mathematical formalism underlying our tools facilitates integrative analysis of data from virtually any sequencing-based functional profile. ..
  28. ncbi request reprint Application of advanced technologies in ageing research
    Ching Aeng Lim
    Gene Regulation Laboratory, Genome Institute of Singapore, Singapore 138672
    Mech Ageing Dev 128:149-60. 2007
    ..We also highlight recent developments of these three technologies and how these developments are applicable to ageing research...
  29. ncbi request reprint Molecular framework underlying pluripotency
    Yuin Han Loh
    Gene Regulation Laboratory, Genome Institute of Singapore, Singapore
    Cell Cycle 7:885-91. 2008
    ..Here, we explore the molecular framework governing pluripotency in the context of pluripotent ES cells and the reprogrammed iPS cells...
  30. doi request reprint Zfp143 regulates Nanog through modulation of Oct4 binding
    Xi Chen
    Genome Institute of Singapore, Singapore
    Stem Cells 26:2759-67. 2008
    ..Disclosure of potential conflicts of interest is found at the end of this article...
  31. doi request reprint Transcriptional and epigenetic regulations of embryonic stem cells
    Jia Hui Ng
    Gene Regulation Laboratory, Genome Institute of Singapore, Singapore 138672, Singapore
    Mutat Res 647:52-8. 2008
    ..Herein, we discuss the recent advancements made in understanding the transcriptional and epigenetic regulations of embryonic stem cells...
  32. pmc Mapping the networks for pluripotency
    Kun Xue
    Gene Regulation Laboratory, Stem Cell and Developmental Biology, Genome Institute of Singapore, 60 Biopolis Street, no 02 01, Genome Building, 138672, Singapore
    Philos Trans R Soc Lond B Biol Sci 366:2238-46. 2011
    ..These studies provide an insight into the defining characteristics as well as the plasticity of pluripotent cells...
  33. ncbi request reprint Klf2 Is an Essential Factor that Sustains Ground State Pluripotency
    Jia Chi Yeo
    Genome Institute of Singapore, 60 Biopolis Street, 02 01 Genome Building, Singapore 138672, Singapore School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore
    Cell Stem Cell 14:864-72. 2014
    ..Collectively, our study defines the Mek/Erk/Klf2 axis that cooperates with the Gsk3/Tcf3/Esrrb pathway in mediating ground state pluripotency. ..
  34. doi request reprint Induction of a human pluripotent state with distinct regulatory circuitry that resembles preimplantation epiblast
    Yun Shen Chan
    Gene Regulation Laboratory, Genome Institute of Singapore, Singapore 138672, Singapore Electronic address
    Cell Stem Cell 13:663-75. 2013
    ..In summary, our study identifies a distinct hESC state with defined regulatory circuitry that will facilitate future analysis of human preimplantation embryogenesis and pluripotency...
  35. doi request reprint Stem cell genome-to-systems biology
    Na Yu Chia
    Gene Regulation Laboratory, Genome Institute of Singapore, Singapore
    Wiley Interdiscip Rev Syst Biol Med 4:39-49. 2012
    ..With the elucidation of protein-protein interaction, protein-DNA regulation, microRNA involvement as well as the epigenetic modifications, it is possible to comprehend the defining features of stem cells at the system level...
  36. doi request reprint FoxO: a new addition to the ESC cartel
    Kevin Andrew Uy Gonzales
    Gene Regulation Laboratory, Genome Institute of Singapore, Singapore 138672
    Cell Stem Cell 9:181-3. 2011
    ..In a recent Nature Cell Biology Letter, Zhang et al. (2011) uncover a novel role for FoxO proteins in regulating the identity of human ESCs...
  37. pmc The transcriptional regulation of pluripotency
    Jia Chi Yeo
    Gene Regulation Laboratory, Genome Institute of Singapore, 60 Biopolis Street, 02 01 Genome Building, Singapore 138672, Singapore
    Cell Res 23:20-32. 2013
    ....
  38. doi request reprint Whole-genome mapping of histone H3 Lys4 and 27 trimethylations reveals distinct genomic compartments in human embryonic stem cells
    Xiao Dong Zhao
    Genome Technology and Biology Group, Genome Institute of Singapore, 138672, Singapore
    Cell Stem Cell 1:286-98. 2007
    ..These global histone methylation maps provide an epigenetic framework that enables the discovery of novel transcriptional networks and delineation of different genetic compartments of the pluripotent cell genome...
  39. pmc Evolution of the mammalian transcription factor binding repertoire via transposable elements
    Guillaume Bourque
    Computational and Mathematical Biology, Genome Institute of Singapore, Singapore 138672, Singapore
    Genome Res 18:1752-62. 2008
    ..Our results demonstrate that transcriptional regulatory networks are highly dynamic in eukaryotic genomes and that transposable elements play an important role in expanding the repertoire of binding sites...
  40. doi request reprint KLF4 and PBX1 directly regulate NANOG expression in human embryonic stem cells
    Ken Kwok Keung Chan
    Stem Cell Group, Bioprocessing Technology Institute, A STAR Agency for Science, Technology and Research, Biopolis, Singapore
    Stem Cells 27:2114-25. 2009
    ..Our results show two novel upstream transcription activators of NANOG that are functionally important for the self-renewal of hESC and provide new insights into the expanded regulatory circuitry that maintains hESC pluripotency...
  41. ncbi request reprint Transcriptional regulatory networks in embryonic stem cells
    Yun Shen Chan
    Gene Regulation Laboratory, Genome Institute of Singapore, 60 Biopolis Street, 02 01, Genome Building, Singapore, Singapore 138672
    Prog Drug Res 67:239-52. 2011
    ..This chapter traces the major efforts made over the past decade in dissecting the transcriptional regulatory network governing ESC identity and offers perspectives on the future directions of the field...
  42. ncbi request reprint Coactivators p300 and CBP Maintain the Identity of Mouse Embryonic Stem Cells by Mediating Long-Range Chromatin Structure
    Fang Fang
    Computation and Systems Biology, Singapore MIT Alliance, Singapore, Singapore
    Stem Cells 32:1805-16. 2014
    ..Stem Cells 2014;32:1805-1816. ..
  43. doi request reprint Choreographing pluripotency and cell fate with transcription factors
    Kevin Andrew Uy Gonzales
    Gene Regulation Laboratory, Genome Institute of Singapore, Singapore 138672, Singapore
    Biochim Biophys Acta 1809:337-49. 2011
    ..Here we review the advancements in the role of transcription factors in pluripotency, as well as in the conversion between and within pluripotent and somatic cell types...
  44. doi request reprint Molecules that promote or enhance reprogramming of somatic cells to induced pluripotent stem cells
    Bo Feng
    Gene Regulation Laboratory, Genome Institute of Singapore, Singapore 138672, Republic of Singapore
    Cell Stem Cell 4:301-12. 2009
    ..Current iPSC studies will provide a paradigm for the combinatorial use of genetic factors and chemicals for the broader applications to alter cellular states of potency...
  45. doi request reprint TGFbeta and SMADs talk to NANOG in human embryonic stem cells
    Jianming Jiang
    Gene Regulation Laboratory, Genome Institute of Singapore, Singapore 138672, Republic of Singapore
    Cell Stem Cell 3:127-8. 2008
    ..In this issue of Cell Stem Cell, Xu et al. (2008) show that this pathway upregulates the expression of a key pluripotency gene NANOG through SMAD2/3...
  46. doi request reprint Driving pluripotency and reprogramming: nuclear receptors at the helm
    Kevin Andrew Uy Gonzales
    Gene Regulation Laboratory, Genome Institute of Singapore, Singapore 138672, Singapore NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, Singapore 117456, Singapore Electronic address
    Semin Cell Dev Biol 24:670-8. 2013
    ....
  47. pmc T-cell factor 3 regulates embryonic stem cell pluripotency and self-renewal by the transcriptional control of multiple lineage pathways
    Wai Leong Tam
    Stem Cell and Developmental Biology, Genome Institute of Singapore, 02 01, Genome, Singapore
    Stem Cells 26:2019-31. 2008
    ..These data demonstrate an important role for Tcf3 in modulating the appropriate level of gene transcription in ESCs and during embryonic development. Disclosure of potential conflicts of interest is found at the end of this article...