Thorsten Wohland

Summary

Affiliation: National University of Singapore
Country: Singapore

Publications

  1. doi request reprint Single plane illumination fluorescence correlation spectroscopy (SPIM-FCS) probes inhomogeneous three-dimensional environments
    Thorsten Wohland
    Department of Chemistry, National University of Singapore, Singapore
    Opt Express 18:10627-41. 2010
  2. pmc Single molecule resolution of the antimicrobial action of quantum dot-labeled sushi peptide on live bacteria
    Sebastian Leptihn
    Singapore MIT Alliance, Singapore
    BMC Biol 7:22. 2009
  3. pmc Determination of dissociation constants in living zebrafish embryos with single wavelength fluorescence cross-correlation spectroscopy
    Xianke Shi
    Department of Chemistry, National University of Singapore, Singapore
    Biophys J 97:678-86. 2009
  4. pmc Diffusion, transport, and cell membrane organization investigated by imaging fluorescence cross-correlation spectroscopy
    Jagadish Sankaran
    Department of Chemistry, National University of Singapore, Singapore
    Biophys J 97:2630-9. 2009
  5. doi request reprint Probing events with single molecule sensitivity in zebrafish and Drosophila embryos by fluorescence correlation spectroscopy
    Xianke Shi
    Department of Chemistry, National University of Singapore, Singapore
    Dev Dyn 238:3156-67. 2009
  6. ncbi request reprint Multifunctional fluorescence correlation microscope for intracellular and microfluidic measurements
    Xiaotao Pan
    NUS Graduate Program in Bioengineering, National University of Singapore, 28 Medical Drive, Singapore 117456, Singapore
    Rev Sci Instrum 78:053711. 2007
  7. ncbi request reprint Recent advances in fluorescence cross-correlation spectroscopy
    Ling Chin Hwang
    Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore, 117543, Singapore
    Cell Biochem Biophys 49:1-13. 2007
  8. ncbi request reprint Spatially resolved total internal reflection fluorescence correlation microscopy using an electron multiplying charge-coupled device camera
    Balakrishnan Kannan
    Department of Chemistry, 3, Science Drive 3, National University of Singapore, Singapore 117543
    Anal Chem 79:4463-70. 2007
  9. pmc Factors affecting the quantification of biomolecular interactions by fluorescence cross-correlation spectroscopy
    Yong Hwee Foo
    Department of Chemistry, National University of Singapore, Singapore
    Biophys J 102:1174-83. 2012
  10. ncbi request reprint Molecular mechanisms that govern the specificity of Sushi peptides for Gram-negative bacterial membrane lipids
    Peng Li
    Department of Biological Sciences, National University of Singapore, Singapore
    Biochemistry 45:10554-62. 2006

Collaborators

Detail Information

Publications32

  1. doi request reprint Single plane illumination fluorescence correlation spectroscopy (SPIM-FCS) probes inhomogeneous three-dimensional environments
    Thorsten Wohland
    Department of Chemistry, National University of Singapore, Singapore
    Opt Express 18:10627-41. 2010
    ..In essence, SPIM-FCS offers new opportunities to observe biomolecular interactions quantitatively and functions in a highly multiplexed manner within a physiologically relevant 3D environment...
  2. pmc Single molecule resolution of the antimicrobial action of quantum dot-labeled sushi peptide on live bacteria
    Sebastian Leptihn
    Singapore MIT Alliance, Singapore
    BMC Biol 7:22. 2009
    ..Despite the increasingly comprehensive understanding of the biochemical properties of these peptides, the actual mechanism by which antimicrobial peptides lyse microbes is controversial...
  3. pmc Determination of dissociation constants in living zebrafish embryos with single wavelength fluorescence cross-correlation spectroscopy
    Xianke Shi
    Department of Chemistry, National University of Singapore, Singapore
    Biophys J 97:678-86. 2009
    ....
  4. pmc Diffusion, transport, and cell membrane organization investigated by imaging fluorescence cross-correlation spectroscopy
    Jagadish Sankaran
    Department of Chemistry, National University of Singapore, Singapore
    Biophys J 97:2630-9. 2009
    ..We investigate the organization and dynamics of SBD-bound lipid microdomains under the conditions of cholesterol removal and cytoskeleton disruption...
  5. doi request reprint Probing events with single molecule sensitivity in zebrafish and Drosophila embryos by fluorescence correlation spectroscopy
    Xianke Shi
    Department of Chemistry, National University of Singapore, Singapore
    Dev Dyn 238:3156-67. 2009
    ..This work provides an approach to study molecular processes in vivo and opens up the possibility to relate these molecular processes to developmental biology questions...
  6. ncbi request reprint Multifunctional fluorescence correlation microscope for intracellular and microfluidic measurements
    Xiaotao Pan
    NUS Graduate Program in Bioengineering, National University of Singapore, 28 Medical Drive, Singapore 117456, Singapore
    Rev Sci Instrum 78:053711. 2007
    ..Two-photon excitation FCS was also realized by coupling a pulsed Ti: sapphire laser into the microscope and used for flow direction characterization in microchannels...
  7. ncbi request reprint Recent advances in fluorescence cross-correlation spectroscopy
    Ling Chin Hwang
    Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore, 117543, Singapore
    Cell Biochem Biophys 49:1-13. 2007
    ..This will introduce biological readers to FCCS and its related techniques and provide a starting point to selecting which experimental scheme is suitable for their type of biological study...
  8. ncbi request reprint Spatially resolved total internal reflection fluorescence correlation microscopy using an electron multiplying charge-coupled device camera
    Balakrishnan Kannan
    Department of Chemistry, 3, Science Drive 3, National University of Singapore, Singapore 117543
    Anal Chem 79:4463-70. 2007
    ..8 ms. This area corresponds to a 40 x 40 pixel region of interest with a dimension of 11.3 x 11.3 microm2 and is sufficiently large to allow the measurement of the lower membrane of a whole cell simultaneously...
  9. pmc Factors affecting the quantification of biomolecular interactions by fluorescence cross-correlation spectroscopy
    Yong Hwee Foo
    Department of Chemistry, National University of Singapore, Singapore
    Biophys J 102:1174-83. 2012
    ..We use this information to correct FCCS measurements of the interaction of Cdc42, a small Rho-GTPase, with its effector IQGAP1 in live cell measurements to obtain a label-independent value for the K(d)...
  10. ncbi request reprint Molecular mechanisms that govern the specificity of Sushi peptides for Gram-negative bacterial membrane lipids
    Peng Li
    Department of Biological Sciences, National University of Singapore, Singapore
    Biochemistry 45:10554-62. 2006
    ....
  11. doi request reprint Study of interaction of hypericin and its pharmaceutical preparation by fluorescence techniques
    Jun Liu
    National University of Singapore, Department of Chemistry, 3 Science Drive 3, Singapore 117543, Singapore
    J Biomed Opt 14:014003. 2009
    ....
  12. doi request reprint Line scan fluorescence correlation spectroscopy for three-dimensional microfluidic flow velocity measurements
    Xiaotao Pan
    National University of Singapore, NUS Graduate Program in Bioengineering, 28 Medical Drive, Singapore 117456, Singapore
    J Biomed Opt 14:024049. 2009
    ..5 microm in all three dimensions. The feasibility of line scan FCS for 3-D microfluidic flow is verified by measurements in microchannels and small blood vessels of zebrafish embryos...
  13. doi request reprint Interaction of an artificial antimicrobial peptide with lipid membranes
    Lanlan Yu
    Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore
    Biochim Biophys Acta 1788:333-44. 2009
    ..The understanding of the mechanism of action of this peptide in relation to the properties of its constituent amino acids is a first step in designing better peptides in the future...
  14. ncbi request reprint Characterization of flow direction in microchannels and zebrafish blood vessels by scanning fluorescence correlation spectroscopy
    Xiaotao Pan
    National University of Singapore, NUS Graduate Program in Bioengineering, 28 Medical Drive, Singapore 117456, Singapore
    J Biomed Opt 12:014034. 2007
    ..To extend the technique to live tissue measurements we demonstrate that line-scan FCS can determine the flow direction in zebrafish small blood vessels in a label-free approach...
  15. doi request reprint The F-techniques: advances in receptor protein studies
    Ping Liu
    Department of Chemistry, National University of Singapore, Singapore
    Trends Endocrinol Metab 19:181-90. 2008
    ....
  16. doi request reprint Molecular diffusion measurement in lipid bilayers over wide concentration ranges: a comparative study
    Lin Guo
    Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore
    Chemphyschem 9:721-8. 2008
    ..Herein, we seek to establish a comparison between FRAP, SPT, FCS, and ITIR-FCS by measuring the lateral diffusion coefficients in two model systems, namely, supported lipid bilayers and giant unilamellar vesicles...
  17. ncbi request reprint Prism-based multicolor fluorescence correlation spectrometer
    Ling Chin Hwang
    Department of Chemistry, National University of Singapore, Singapore
    Opt Lett 31:1310-2. 2006
    ..This spectrometer offers high optical stability and no focal volume mismatch for the multicolor detection of molecular dynamics and interactions, with single-molecule sensitivity...
  18. ncbi request reprint The molecular mechanism of interaction between sushi peptide and Pseudomonas endotoxin
    Peng Li
    Department of Biological Sciences, National University of Singapore, Singapore
    Cell Mol Immunol 3:21-8. 2006
    ..Furthermore, in the presence of LPS, the peptide transitions from a random structure into an alpha-helical conformation and it disrupts LPS aggregates, hence, neutralizing the LPS toxicity...
  19. doi request reprint A bioelectronic platform using a graphene-lipid bilayer interface
    Priscilla Kailian Ang
    Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543
    ACS Nano 4:7387-94. 2010
    ..Here, we demonstrate that the bactericidal activity of antimicrobial peptides can be sensed electrically by graphene based on a complex interplay of biomolecular doping and ionic screening effect...
  20. ncbi request reprint EGFR activation monitored by SW-FCCS in live cells
    Xiaoxiao Ma
    Department of Chemistry, National University of Singapore, Singapore
    Front Biosci (Elite Ed) 3:22-32. 2011
    ..After EGF stimulation the interaction between PTB and EGFR increases significantly in a time dependent manner...
  21. doi request reprint Calibration and limits of camera-based fluorescence correlation spectroscopy: a supported lipid bilayer study
    Nirmalya Bag
    Department of Biological Sciences and NUS Centre for Bio Imaging Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117546, Singapore
    Chemphyschem 13:2784-94. 2012
    ....
  22. doi request reprint Developing in vivo biophysics by fishing for single molecules
    Xi Wang
    Department of Chemistry, National University of Singapore, Singapore
    Dev Biol 347:1-8. 2010
    ..This review aims to define SMTs applicable in developmental biology, and discuss properties of an ideal animal model...
  23. ncbi request reprint Effects of N-methyl pyrrolidone on the uptake of hypericin in human bladder carcinoma and co-staining with DAPI investigated by confocal microscopy
    Constance Lay Lay Saw
    Department of Pharmacy, National University of Singapore, 18 Science Drive 4, Singapore, Singapore 117543
    Technol Cancer Res Treat 6:383-94. 2007
    ..Thus, NMP was able to enhance the photodynamic therapeutic effects of HY on cancer cells...
  24. pmc Requirement of vasculogenesis and blood circulation in late stages of liver growth in zebrafish
    Svetlana Korzh
    Department of Biological Sciences, National University of Singapore, Singapore
    BMC Dev Biol 8:84. 2008
    ..In zebrafish, although it has been found that ECs are not required for liver budding, how and when the spatio-temporal pattern of liver growth is coordinated with ECs remains to be elucidated...
  25. doi request reprint The performance of 2D array detectors for light sheet based fluorescence correlation spectroscopy
    Anand Pratap Singh
    Department of Biological Sciences and NUS Centre for Bio Imaging Sciences, National University of Singapore, 14 Science Drive 4, 117557, Singapore
    Opt Express 21:8652-68. 2013
    ..While EMCCD cameras have a temporal resolution in the millisecond range, sCMOS cameras and SPAD arrays can extend the time resolution of SPIM-FCS down to 10 μs or lower...
  26. doi request reprint Fluorescence cross-correlation spectroscopy (FCCS) in living cells
    Xiaoxiao Ma
    Faculty of Science, Department of Chemistry, National University of Singapore, Singapore, Singapore
    Methods Mol Biol 1076:557-73. 2014
    ..Here, we describe the procedures to apply FCCS in various biological samples ranging from live cells to in vivo measurements, with the focus on DC-FCCS and SW-FCCS. ..
  27. doi request reprint Colloidal crystals from surface-tension-assisted self-assembly: a novel matrix for single-molecule experiments
    Wen Cong Yeon
    Information Storage Materials Laboratory, Department of Electrical and Computer Engineering, National University of Singapore, Singapore
    Langmuir 24:12142-9. 2008
    ..This shows that colloidal crystals can be used to test the function of single molecules of enzymes and protein under controlled spatial confinement...
  28. pmc Simultaneous multicolor fluorescence cross-correlation spectroscopy to detect higher order molecular interactions using single wavelength laser excitation
    Ling Chin Hwang
    National University of Singapore, Department of Chemistry, Singapore 117543, Singapore
    Biophys J 91:715-27. 2006
    ..Performed with appropriate negative controls, SW-FCCS can determine interaction patterns between ligands and receptors...
  29. doi request reprint Membrane destabilization by monomeric hIAPP observed by imaging fluorescence correlation spectroscopy
    Nirmalya Bag
    Departments of Biological Sciences and Chemistry and NUS Centre for Bio Imaging Sciences CBIS, National University of Singapore, 117557, Singapore
    Chem Commun (Camb) 49:9155-7. 2013
    ....
  30. pmc Phosphatidylserine dynamics in cellular membranes
    Jason G Kay
    Cell Biology, The Hospital for Sick Children, Toronto, ON, Canada
    Mol Biol Cell 23:2198-212. 2012
    ..Our data provide new insights into properties of PS inside cells and suggest mechanisms to account for the subcellular distribution and function of this phospholipid...
  31. pmc Investigation of the dimerization of proteins from the epidermal growth factor receptor family by single wavelength fluorescence cross-correlation spectroscopy
    Ping Liu
    Department of Chemistry, National University of Singapore, Singapore
    Biophys J 93:684-98. 2007
    ..These receptors are major targets of anti-cancer drug development, and the receptors' homo- and heterodimeric structures are relevant for such developments...
  32. ncbi request reprint Investigation of a novel artificial antimicrobial peptide by fluorescence correlation spectroscopy: an amphipathic cationic pattern is sufficient for selective binding to bacterial type membranes and antimicrobial activity
    Lanlan Yu
    Department of Chemistry, National University of Singapore, Singapore
    Biochim Biophys Acta 1716:29-39. 2005
    ..The identification of the amphipathic cationic pattern as the mediator of selectivity and antimicrobial activity could be a first step in the rational design of better antimicrobial peptides...