Research Topics
| Thorsten WohlandSummaryAffiliation: National University of Singapore Country: Singapore Publications
| Collaborators
|
Detail Information
Publications
Single plane illumination fluorescence correlation spectroscopy (SPIM-FCS) probes inhomogeneous three-dimensional environmentsThorsten Wohland
Department of Chemistry, National University of Singapore, Singapore
Opt Express 18:10627-41. 2010..In essence, SPIM-FCS offers new opportunities to observe biomolecular interactions quantitatively and functions in a highly multiplexed manner within a physiologically relevant 3D environment...
Single molecule resolution of the antimicrobial action of quantum dot-labeled sushi peptide on live bacteriaSebastian Leptihn
Singapore MIT Alliance, Singapore
BMC Biol 7:22. 2009..Despite the increasingly comprehensive understanding of the biochemical properties of these peptides, the actual mechanism by which antimicrobial peptides lyse microbes is controversial...- Determination of dissociation constants in living zebrafish embryos with single wavelength fluorescence cross-correlation spectroscopyXianke Shi
Department of Chemistry, National University of Singapore, Singapore
Biophys J 97:678-86. 2009.... - Probing events with single molecule sensitivity in zebrafish and Drosophila embryos by fluorescence correlation spectroscopyXianke Shi
Department of Chemistry, National University of Singapore, Singapore
Dev Dyn 238:3156-67. 2009..This work provides an approach to study molecular processes in vivo and opens up the possibility to relate these molecular processes to developmental biology questions... - Diffusion, transport, and cell membrane organization investigated by imaging fluorescence cross-correlation spectroscopyJagadish Sankaran
Department of Chemistry, National University of Singapore, Singapore
Biophys J 97:2630-9. 2009..We investigate the organization and dynamics of SBD-bound lipid microdomains under the conditions of cholesterol removal and cytoskeleton disruption... 
Multifunctional fluorescence correlation microscope for intracellular and microfluidic measurementsXiaotao Pan
NUS Graduate Program in Bioengineering, National University of Singapore, 28 Medical Drive, Singapore 117456, Singapore
Rev Sci Instrum 78:053711. 2007..Two-photon excitation FCS was also realized by coupling a pulsed Ti: sapphire laser into the microscope and used for flow direction characterization in microchannels...- Recent advances in fluorescence cross-correlation spectroscopyLing Chin Hwang
Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore, 117543, Singapore
Cell Biochem Biophys 49:1-13. 2007..This will introduce biological readers to FCCS and its related techniques and provide a starting point to selecting which experimental scheme is suitable for their type of biological study... - Spatially resolved total internal reflection fluorescence correlation microscopy using an electron multiplying charge-coupled device cameraBalakrishnan Kannan
Department of Chemistry, 3, Science Drive 3, National University of Singapore, Singapore 117543
Anal Chem 79:4463-70. 2007..8 ms. This area corresponds to a 40 x 40 pixel region of interest with a dimension of 11.3 x 11.3 microm2 and is sufficiently large to allow the measurement of the lower membrane of a whole cell simultaneously... - Factors affecting the quantification of biomolecular interactions by fluorescence cross-correlation spectroscopyYong Hwee Foo
Department of Chemistry, National University of Singapore, Singapore
Biophys J 102:1174-83. 2012..We use this information to correct FCCS measurements of the interaction of Cdc42, a small Rho-GTPase, with its effector IQGAP1 in live cell measurements to obtain a label-independent value for the K(d)... - Study of interaction of hypericin and its pharmaceutical preparation by fluorescence techniquesJun Liu
National University of Singapore, Department of Chemistry, 3 Science Drive 3, Singapore 117543, Singapore
J Biomed Opt 14:014003. 2009.... - Molecular mechanisms that govern the specificity of Sushi peptides for Gram-negative bacterial membrane lipidsPeng Li
Department of Biological Sciences, National University of Singapore, Singapore
Biochemistry 45:10554-62. 2006.... - Line scan fluorescence correlation spectroscopy for three-dimensional microfluidic flow velocity measurementsXiaotao Pan
National University of Singapore, NUS Graduate Program in Bioengineering, 28 Medical Drive, Singapore 117456, Singapore
J Biomed Opt 14:024049. 2009..5 microm in all three dimensions. The feasibility of line scan FCS for 3-D microfluidic flow is verified by measurements in microchannels and small blood vessels of zebrafish embryos... - Interaction of an artificial antimicrobial peptide with lipid membranesLanlan Yu
Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore
Biochim Biophys Acta 1788:333-44. 2009..The understanding of the mechanism of action of this peptide in relation to the properties of its constituent amino acids is a first step in designing better peptides in the future... - Prism-based multicolor fluorescence correlation spectrometerLing Chin Hwang
Department of Chemistry, National University of Singapore, Singapore
Opt Lett 31:1310-2. 2006..This spectrometer offers high optical stability and no focal volume mismatch for the multicolor detection of molecular dynamics and interactions, with single-molecule sensitivity... - Molecular diffusion measurement in lipid bilayers over wide concentration ranges: a comparative studyLin Guo
Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore
Chemphyschem 9:721-8. 2008..Herein, we seek to establish a comparison between FRAP, SPT, FCS, and ITIR-FCS by measuring the lateral diffusion coefficients in two model systems, namely, supported lipid bilayers and giant unilamellar vesicles... - Characterization of flow direction in microchannels and zebrafish blood vessels by scanning fluorescence correlation spectroscopyXiaotao Pan
National University of Singapore, NUS Graduate Program in Bioengineering, 28 Medical Drive, Singapore 117456, Singapore
J Biomed Opt 12:014034. 2007..To extend the technique to live tissue measurements we demonstrate that line-scan FCS can determine the flow direction in zebrafish small blood vessels in a label-free approach... - The F-techniques: advances in receptor protein studiesPing Liu
Department of Chemistry, National University of Singapore, Singapore
Trends Endocrinol Metab 19:181-90. 2008.... - A bioelectronic platform using a graphene-lipid bilayer interfacePriscilla Kailian Ang
Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543
ACS Nano 4:7387-94. 2010..Here, we demonstrate that the bactericidal activity of antimicrobial peptides can be sensed electrically by graphene based on a complex interplay of biomolecular doping and ionic screening effect... - EGFR activation monitored by SW-FCCS in live cellsXiaoxiao Ma
Department of Chemistry, National University of Singapore, Singapore
Front Biosci (Elite Ed) 3:22-32. 2011..After EGF stimulation the interaction between PTB and EGFR increases significantly in a time dependent manner... - The molecular mechanism of interaction between sushi peptide and Pseudomonas endotoxinPeng Li
Department of Biological Sciences, National University of Singapore, Singapore
Cell Mol Immunol 3:21-8. 2006..Furthermore, in the presence of LPS, the peptide transitions from a random structure into an alpha-helical conformation and it disrupts LPS aggregates, hence, neutralizing the LPS toxicity... - Calibration and limits of camera-based fluorescence correlation spectroscopy: a supported lipid bilayer studyNirmalya Bag
Department of Biological Sciences and NUS Centre for Bio Imaging Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117546, Singapore
Chemphyschem 13:2784-94. 2012.... - Developing in vivo biophysics by fishing for single moleculesXi Wang
Department of Chemistry, National University of Singapore, Singapore
Dev Biol 347:1-8. 2010..This review aims to define SMTs applicable in developmental biology, and discuss properties of an ideal animal model... - Requirement of vasculogenesis and blood circulation in late stages of liver growth in zebrafishSvetlana Korzh
Department of Biological Sciences, National University of Singapore, Singapore
BMC Dev Biol 8:84. 2008..In zebrafish, although it has been found that ECs are not required for liver budding, how and when the spatio-temporal pattern of liver growth is coordinated with ECs remains to be elucidated... - Effects of N-methyl pyrrolidone on the uptake of hypericin in human bladder carcinoma and co-staining with DAPI investigated by confocal microscopyConstance Lay Lay Saw
Department of Pharmacy, National University of Singapore, 18 Science Drive 4, Singapore, Singapore 117543
Technol Cancer Res Treat 6:383-94. 2007..Thus, NMP was able to enhance the photodynamic therapeutic effects of HY on cancer cells... - Colloidal crystals from surface-tension-assisted self-assembly: a novel matrix for single-molecule experimentsWen Cong Yeon
Information Storage Materials Laboratory, Department of Electrical and Computer Engineering, National University of Singapore, Singapore
Langmuir 24:12142-9. 2008..This shows that colloidal crystals can be used to test the function of single molecules of enzymes and protein under controlled spatial confinement... - Simultaneous multicolor fluorescence cross-correlation spectroscopy to detect higher order molecular interactions using single wavelength laser excitationLing Chin Hwang
National University of Singapore, Department of Chemistry, Singapore 117543, Singapore
Biophys J 91:715-27. 2006..Performed with appropriate negative controls, SW-FCCS can determine interaction patterns between ligands and receptors... - Phosphatidylserine dynamics in cellular membranesJason G Kay
Cell Biology, The Hospital for Sick Children, Toronto, ON, Canada
Mol Biol Cell 23:2198-212. 2012..Our data provide new insights into properties of PS inside cells and suggest mechanisms to account for the subcellular distribution and function of this phospholipid... - Investigation of the dimerization of proteins from the epidermal growth factor receptor family by single wavelength fluorescence cross-correlation spectroscopyPing Liu
Department of Chemistry, National University of Singapore, Singapore
Biophys J 93:684-98. 2007..These receptors are major targets of anti-cancer drug development, and the receptors' homo- and heterodimeric structures are relevant for such developments... - Investigation of a novel artificial antimicrobial peptide by fluorescence correlation spectroscopy: an amphipathic cationic pattern is sufficient for selective binding to bacterial type membranes and antimicrobial activityLanlan Yu
Department of Chemistry, National University of Singapore, Singapore
Biochim Biophys Acta 1716:29-39. 2005..The identification of the amphipathic cationic pattern as the mediator of selectivity and antimicrobial activity could be a first step in the rational design of better antimicrobial peptides...