K S Tan

Summary

Affiliation: National University of Singapore
Country: Singapore

Publications

  1. ncbi request reprint Recent advances in Blastocystis hominis research: hot spots in terra incognita
    Kevin S W Tan
    Department of Microbiology, Faculty of Medicine, National University of Singapore, 5 Science Drive 2, Singapore 117597
    Int J Parasitol 32:789-804. 2002
  2. ncbi request reprint Description of a new species of Thais (Mollusca: Neogastropoda: Muricidae) from Taiwan, based on morphological and allozyme analyses
    K S Tan
    Tropical Marine Science Institute, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260
    Zoolog Sci 18:1275-89. 2001
  3. ncbi request reprint Do Blastocystis hominis colony forms undergo programmed cell death?
    K S Tan
    Department of Microbiology, Faculty of Medicine, National University of Singapore, Singapore
    Parasitol Res 87:362-7. 2001
  4. pmc A metronidazole-resistant isolate of Blastocystis spp. is susceptible to nitric oxide and downregulates intestinal epithelial inducible nitric oxide synthase by a novel parasite survival mechanism
    Haris Mirza
    Laboratory of Molecular and Cellular Parasitology, Department of Microbiology, Yong Loo Lin School of Medicine, National University of Singapore, 5 Science Drive 2, Singapore 117596, Singapore
    Infect Immun 79:5019-26. 2011
  5. pmc New insights on classification, identification, and clinical relevance of Blastocystis spp
    Kevin S W Tan
    Department of Microbiology, Laboratory of Molecular and Cellular Parasitology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Republic of Singapore
    Clin Microbiol Rev 21:639-65. 2008
  6. ncbi request reprint Protozoan programmed cell death--insights from Blastocystis deathstyles
    Kevin S W Tan
    Laboratory of Molecular and Cellular Parasitology, Department of Microbiology, Yong Loo Lin School of Medicine, National University of Singapore, 5 Science Drive 2, Singapore 117597
    Trends Parasitol 21:547-50. 2005
  7. ncbi request reprint Blastocystis in humans and animals: new insights using modern methodologies
    Kevin S W Tan
    Laboratory of Molecular and Cellular Parasitology, Department of Microbiology, Faculty of Medicine, National University of Singapore, 5 Science Drive 2, S 117597 Singapore, Singapore
    Vet Parasitol 126:121-44. 2004
  8. ncbi request reprint Blastocystis hominis: evidence for caspase-3-like activity in cells undergoing programmed cell death
    A M Nasirudeen
    Department of Microbiology, Faculty of Medicine, National University of Singapore, Singapore
    Parasitol Res 87:559-65. 2001
  9. ncbi request reprint Exposure of Blastocystis species to a cytotoxic monoclonal antibody
    K S Tan
    Department of Microbiology, Faculty of Medicine, National University of Singapore, Singapore
    Parasitol Res 87:534-8. 2001
  10. ncbi request reprint Programmed cell death in a human intestinal parasite, Blastocystis hominis
    A M Nasirudeen
    Department of Microbiology, Faculty of Medicine, National University of Singapore, Singapore
    Parasitology 123:235-46. 2001

Collaborators

  • KKN Namboodri
  • M Singh
  • B G Stiles
  • NIGEL R YARLETT
  • Hisao Yoshikawa
  • R C Andrew Thompson
  • Rebecca Traub
  • Haris Mirza
  • A M A Nasirudeen
  • Manoj K Puthia
  • Jing Yin
  • Jia Lu
  • Selena W S Sio
  • R P Samy
  • A M Nasirudeen
  • Kenneth H S Wong
  • Yelena Lantsman
  • C Rune Stensvold
  • K S Girish
  • E G Rowan
  • P Gopalakrishnakone
  • G Sethi
  • V T K Chow
  • E H Yap
  • S N Swamy
  • M Hemshekhar
  • Joshua D W Teo
  • Zhaona Wu
  • Fahad Kidwai
  • Jacqui Upcroft
  • Josephine Howe
  • Angeline J J Ye
  • Raymond T P Lin
  • G C Ng
  • Mary Morada
  • Mark B Taylor
  • C Graham Clark
  • Eric Viscogliosi
  • G Kumar Suresh
  • Alex S Y Lee
  • Aparna Vaithilingam
  • Yap Eu Hian

Detail Information

Publications30

  1. ncbi request reprint Recent advances in Blastocystis hominis research: hot spots in terra incognita
    Kevin S W Tan
    Department of Microbiology, Faculty of Medicine, National University of Singapore, 5 Science Drive 2, Singapore 117597
    Int J Parasitol 32:789-804. 2002
    ..Another area that requires attention is the development of methods for the transfection of foreign/altered genes into B. hominis in order to facilitate genetic experiments...
  2. ncbi request reprint Description of a new species of Thais (Mollusca: Neogastropoda: Muricidae) from Taiwan, based on morphological and allozyme analyses
    K S Tan
    Tropical Marine Science Institute, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260
    Zoolog Sci 18:1275-89. 2001
    ..luteostoma was more closely related to T. keluo than to T. clavigera. However, both analyses indicated the close relationship amongst T. clavigera, T. luteostoma and the new species in relation to T. rufotincta...
  3. ncbi request reprint Do Blastocystis hominis colony forms undergo programmed cell death?
    K S Tan
    Department of Microbiology, Faculty of Medicine, National University of Singapore, Singapore
    Parasitol Res 87:362-7. 2001
    ..Other unusual features included margination of chromatin material and distinct blebbing of nuclei. These ultrastructural features suggest that B. hominis colony forms perhaps undergo a form of programmed cell death...
  4. pmc A metronidazole-resistant isolate of Blastocystis spp. is susceptible to nitric oxide and downregulates intestinal epithelial inducible nitric oxide synthase by a novel parasite survival mechanism
    Haris Mirza
    Laboratory of Molecular and Cellular Parasitology, Department of Microbiology, Yong Loo Lin School of Medicine, National University of Singapore, 5 Science Drive 2, Singapore 117596, Singapore
    Infect Immun 79:5019-26. 2011
    ..This is the first study to highlight a strain-to-strain variation in the ability of Blastocystis to evade the host antiparasitic NO response...
  5. pmc New insights on classification, identification, and clinical relevance of Blastocystis spp
    Kevin S W Tan
    Department of Microbiology, Laboratory of Molecular and Cellular Parasitology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Republic of Singapore
    Clin Microbiol Rev 21:639-65. 2008
    ..This review focuses on such recent advances and also provides updates on laboratory and clinical aspects of Blastocystis spp...
  6. ncbi request reprint Protozoan programmed cell death--insights from Blastocystis deathstyles
    Kevin S W Tan
    Laboratory of Molecular and Cellular Parasitology, Department of Microbiology, Yong Loo Lin School of Medicine, National University of Singapore, 5 Science Drive 2, Singapore 117597
    Trends Parasitol 21:547-50. 2005
    ..Recent observations of PCD pathways in Blastocystis suggest that this protozoan, as is the case with its multicellular counterparts, possesses complex cell-death mechanisms...
  7. ncbi request reprint Blastocystis in humans and animals: new insights using modern methodologies
    Kevin S W Tan
    Laboratory of Molecular and Cellular Parasitology, Department of Microbiology, Faculty of Medicine, National University of Singapore, 5 Science Drive 2, S 117597 Singapore, Singapore
    Vet Parasitol 126:121-44. 2004
    ..Application of modern molecular tools has advanced knowledge of the organism's genetic diversity, taxonomy and zoonotic potential...
  8. ncbi request reprint Blastocystis hominis: evidence for caspase-3-like activity in cells undergoing programmed cell death
    A M Nasirudeen
    Department of Microbiology, Faculty of Medicine, National University of Singapore, Singapore
    Parasitol Res 87:559-65. 2001
    ..These findings suggest that caspase-3-like proteases play an important role in B. hominis undergoing PCD, similar to the phenomenon in higher eukaryotic organisms...
  9. ncbi request reprint Exposure of Blastocystis species to a cytotoxic monoclonal antibody
    K S Tan
    Department of Microbiology, Faculty of Medicine, National University of Singapore, Singapore
    Parasitol Res 87:534-8. 2001
    ..Acridine orange staining performed on both exposed and unexposed cells showed similar internal structures when viewed under fluorescence microscopy...
  10. ncbi request reprint Programmed cell death in a human intestinal parasite, Blastocystis hominis
    A M Nasirudeen
    Department of Microbiology, Faculty of Medicine, National University of Singapore, Singapore
    Parasitology 123:235-46. 2001
    ..Our study also ascribes a novel function for the B. hominis central vacuole in apoptosis; it acts as a repository where apoptotic bodies are stored before being released into the extracellular space...
  11. pmc Blastocystis ratti contains cysteine proteases that mediate interleukin-8 response from human intestinal epithelial cells in an NF-kappaB-dependent manner
    Manoj K Puthia
    Laboratory of Molecular and Cellular Parasitology, Department of Microbiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, 5 Science Drive 2, Singapore 117597
    Eukaryot Cell 7:435-43. 2008
    ..Our findings will contribute to an understanding of the pathobiology of a poorly studied parasite whose public health importance is increasingly recognized...
  12. ncbi request reprint Programmed cell death in Blastocystis hominis occurs independently of caspase and mitochondrial pathways
    A M A Nasirudeen
    Laboratory of Molecular and Cellular Parasitology, Department of Microbiology, Faculty of Medicine, National University of Singapore, 5 Science Drive 2, Singapore 117597, Singapore
    Biochimie 87:489-97. 2005
    ..This study sheds new light on the conserved nature of PCD pathways in parasitic protozoa and is also the first report describing caspase- and mitochondria-independent cell death pathways in a protozoan parasite...
  13. ncbi request reprint Caspase-3-like protease influences but is not essential for DNA fragmentation in Blastocystis undergoing apoptosis
    A M A Nasirudeen
    Laboratory of Molecular and Cellular Parasitology, Department of Microbiology, Faculty of Medicine, National University of Singapore, Singapore
    Eur J Cell Biol 83:477-82. 2004
    ..hominis is not wholly dependent on caspase-3-like protease activity and is associated with mitochondrial dysregulation. This is the first report showing evidence for complex apoptotic pathways in a unicellular parasite...
  14. ncbi request reprint Isolation and characterization of the mitochondrion-like organelle from Blastocystis hominis
    A M A Nasirudeen
    Laboratory of Molecular and Cellular Parasitology, Department of Microbiology, Faculty of Medicine, National University of Singapore, 5 Science Drive 2, 117597 Singapore
    J Microbiol Methods 58:101-9. 2004
    ..The role of MLO in this anaerobic protozoan can be widely investigated using this protocol...
  15. ncbi request reprint Metronidazole induces programmed cell death in the protozoan parasite Blastocystis hominis
    A M A Nasirudeen
    Department of Microbiology, Faculty of Medicine, National University of Singapore, 5 Science Drive 2, Singapore 117597
    Microbiology 150:33-43. 2004
    ..hominis central vacuole in PCD; it acts as a repository where apoptotic bodies are stored before being released into the extracellular space. The implications and possible roles of PCD in B. hominis are discussed...
  16. doi request reprint Staurosporine-induced programmed cell death in Blastocystis occurs independently of caspases and cathepsins and is augmented by calpain inhibition
    Jing Yin
    Laboratory of Molecular and Cellular Parasitology, Department of Microbiology, Yong Loo Lin School of Medicine, National University of Singapore, 5 Science Drive 2, Singapore
    Microbiology 156:1284-93. 2010
    ..While the identities of the proteases responsible for STS-induced apoptosis warrant further investigation, these findings demonstrate that programmed cell death in Blastocystis is complex and regulated by multiple mediators...
  17. ncbi request reprint Snake venom phospholipases A(2): a novel tool against bacterial diseases
    R P Samy
    Infectious Diseases Programme, Department of Microbiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117597
    Curr Med Chem 19:6150-62. 2012
    ..In fact, the C-terminal cationic/hydrophobic segment (residues 115-129) of svPLA(2)s is bactericidal. Thus identification of the bactericidal sites in svPLA(2)s has potential for developing novel antimicrobials...
  18. ncbi request reprint Evidence for holin function of tcdE gene in the pathogenicity of Clostridium difficile
    K S Tan
    Department of Microbiology, Faculty of Medicine, National University of Singapore, Singapore
    J Med Microbiol 50:613-9. 2001
    ..TcdE may function as a lytic protein to facilitate the release of TcdA and TcdB to the extracellular environment, as these toxins lack signal peptide...
  19. ncbi request reprint Predominance of subtype 3 among Blastocystis isolates from a major hospital in Singapore
    Kenneth H S Wong
    Laboratory of Molecular and Cellular Parasitology, National University of Singapore, Singapore 5 Science Drive 2, Singapore 117597, Singapore
    Parasitol Res 102:663-70. 2008
    ..The predominance of subtype 3 in an urbanized city state such as Singapore is in agreement with the idea that subtype 3 is a genotype of human origin...
  20. ncbi request reprint Degradation of human secretory immunoglobulin A by Blastocystis
    Manoj K Puthia
    Laboratory of Molecular and Cellular Parasitology, Department of Microbiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, 117597
    Parasitol Res 97:386-9. 2005
    ..hominis B isolate and aspartic proteinase activity in B. ratii WR1 isolate. These findings suggest that Blastocystis proteases may play a role in parasite survival in vivo...
  21. ncbi request reprint Bacteroides forsythus prtH genotype in periodontitis patients: occurrence and association with periodontal disease
    K S Tan
    Department of Microbiology, Faculty of Medicine, National University of Singapore, Singapore
    J Periodontal Res 36:398-403. 2001
    ..forsythus, for a more accurate determination of the prevalence of periodontal health and disease in epidemiological studies and clinical screening...
  22. pmc A rapid, high-throughput viability assay for Blastocystis spp. reveals metronidazole resistance and extensive subtype-dependent variations in drug susceptibilities
    Haris Mirza
    Laboratory of Molecular and Cellular Parasitology, Department of Microbiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore
    Antimicrob Agents Chemother 55:637-48. 2011
    ..Our study highlights the need to reevaluate established treatment regimens for Blastocystis infections and offers clear new treatment options for Mz treatment failures...
  23. pmc Blastocystis ratti induces contact-independent apoptosis, F-actin rearrangement, and barrier function disruption in IEC-6 cells
    Manoj K Puthia
    Laboratory of Molecular and Cellular Parasitology, Department of Microbiology, Yong Loo Lin School of Medicine, National University of Singapore, 5 Science Drive 2, Singapore 117597, Singapore
    Infect Immun 74:4114-23. 2006
    ....
  24. doi request reprint Autophagy is involved in starvation response and cell death in Blastocystis
    Jing Yin
    Laboratory of Molecular and Cellular Parasitology, Department of Microbiology, Yong Loo Lin School of Medicine, National University of Singapore, 5 Science Drive 2, Singapore
    Microbiology 156:665-77. 2010
    ..fmk. Taken together, our results suggest that the core machinery for autophagy is conserved in Blastocystis, and that it plays an important role in the starvation response and cell death of the parasite...
  25. doi request reprint Blastocystis exhibits inter- and intra-subtype variation in cysteine protease activity
    Haris Mirza
    Laboratory of Molecular and Cellular Parasitology, Department of Microbiology, Yong Loo Lin School of Medicine, National University of Singapore, 5 Science Drive 2, Kent Ridge 117597, Singapore
    Parasitol Res 104:355-61. 2009
    ..Cysteine protease activity and parasite cell size varied over time within cultures of the same isolate. An association between parasite cell size and protease activity was observed...
  26. ncbi request reprint Protease activity of Blastocystis hominis
    Selena W S Sio
    Laboratory of Molecular and Cellular Parasitology, Department of Microbiology, Yong Loo Lin School of Medicine, National University of Singapore, 5 Science Drive 2, Singapore 117597, Singapore
    Parasitol Res 99:126-30. 2006
    ..Proteases were found to be pH-dependent and highest proteolytic activity was observed at neutral pH. Inhibition studies showed that B. hominis isolate B, like many other protozoan parasites, contains mainly cysteine proteases...
  27. ncbi request reprint Multiple reproductive processes in Blastocystis: proceed with caution
    Kevin S W Tan
    Trends Parasitol 19:290-1; author reply 291-2. 2003
  28. doi request reprint Biochemical characterization of a mitochondrial-like organelle from Blastocystis sp. subtype 7
    Yelena Lantsman
    Haskins Laboratories, Pace University, New York, NY 10038, USA
    Microbiology 154:2757-66. 2008
    ..Based upon the results of this study, the Blastocystis organelle falls into the category of a MLO...
  29. ncbi request reprint Ultrastructural and phylogenetic studies on Blastocystis isolates from cockroaches
    Hisao Yoshikawa
    Department of Biological Sciences, Faculty of Science, Nara Women s University, Kitauoya Nishimachi, Nara 630 8506, Japan
    J Eukaryot Microbiol 54:33-7. 2007
    ....
  30. ncbi request reprint Terminology for Blastocystis subtypes--a consensus
    C Rune Stensvold
    Laboratory of Parasitology, Department of Bacteriology, Mycology and Parasitology, Statens Serum Institut, Artillerivej 5, DK 2300 Copenhagen S, Denmark
    Trends Parasitol 23:93-6. 2007
    ..Based primarily on published small-subunit ribosomal RNA gene analyses, we propose that all mammalian and avian isolates should be designated Blastocystis sp. and assigned to one of nine subtypes...