Research Topics
Genomes and GenesSpecies | M-L NgSummaryAffiliation: National University of Singapore Country: Singapore Publications
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Detail Information
Publications
Molecular profiling of T-helper immune genes during dengue virus infectionJincheng Chen
Department of Microbiology, Yong Loo Lin School of Medicine, 5 Science Drive 2, National University of Singapore, 117597 Singapore
Virol J 5:165. 2008..Profiling genes obtained from this study may serve as potential biomarkers and the modulation of Th immune responses during dengue virus infection has important implications in disease outcome...
Transport and budding at two distinct sites of visible nucleocapsids of West Nile (Sarafend) virusM L Ng
Department of Microbiology, National University of Singapore, Singapore
J Med Virol 65:758-64. 2001..Although maturation at the plasma membrane was the dominant mode, during late infection, intracellular maturation into large vacuoles was also observed...
Topographic changes in SARS coronavirus-infected cells at late stages of infectionM L Ng
Department of Microbiology, National University of Singapore, Singapore
Emerg Infect Dis 10:1907-14. 2004..Thickening of the actin filaments at the cell edge provided the bending force to extrude the virus particles...
Proliferative growth of SARS coronavirus in Vero E6 cellsM L Ng
Department of Microbiology, 5 Science Drive 2, National University of Singapore, 117597, Singapore
J Gen Virol 84:3291-303. 2003..By 24 to 30 h p.i., crystalline arrays of the extracellular virus particles were seen commonly at the cell surface...
Early events of SARS coronavirus infection in vero cellsM L Ng
Department of Microbiology, Faculty of Medicine, 5 Science Drive 2, National University of Singapore, Singapore 117597
J Med Virol 71:323-31. 2003..These could be the virus genomes together with the helical nucleocapsids. They were no longer in large vacuoles but packaged into smaller vacuoles in the cytoplasm, and occasionally in small groups...
Inhibition of human colon carcinoma development by lentinan from shiitake mushrooms (Lentinus edodes)Mah Lee Ng
Department of Microbiology, National University of Singapore, Singapore
J Altern Complement Med 8:581-9. 2002..The goal is to document whether the efficacy of the antitumor property is still expressed through this route of administration...
The glycosylation site in the envelope protein of West Nile virus (Sarafend) plays an important role in replication and maturation processesJ Li
Flavivirology Laboratory, Department of Microbiology, 5 Science Drive 2, National University of Singapore, Singapore 117597
J Gen Virol 87:613-22. 2006..This defect was corrected by the expression of wild-type envelope protein in trans. The pWNS-S154A virus matured intracellularly instead of at the plasma membrane as shown for the parental WN(S)V...
Identifying the region influencing the cis-mode of maturation of West Nile (Sarafend) virus using chimeric infectious clonesJ Li
Flavivirology Laboratory, Department of Microbiology, National University of Singapore, Singapore 117597, Singapore
Biochem Biophys Res Commun 334:714-20. 2005..Transmission electron microscopy analyses performed in Vero cells infected with these chimeric viruses disclosed that the 5' end of the WN(S)V genome plays a major role in influencing the process of maturation at the plasma membrane...
Inhibition of West Nile virus entry by using a recombinant domain III from the envelope glycoproteinJ J H Chu
Flavivirology Laboratory, Department of Microbiology, 5 Science Drive 2, National University of Singapore, Singapore 117597, Singapore
J Gen Virol 86:405-12. 2005..The data also suggest that similar attachment molecule(s) or receptor(s) were used by WNV and Den 2 virus for entry into C6/36 mosquito cells...
Immunization of flavivirus West Nile recombinant envelope domain III protein induced specific immune response and protection against West Nile virus infectionJang Hann J Chu
Flavivirology Laboratory, Department of Microbiology, 5 Science Drive 2, National University of Singapore, Singapore 117597
J Immunol 178:2699-705. 2007..Overall, this study highlighted that recombinant WNV E DIII protein delivered in combination with CpG adjuvant to mice generated a Th1 immune response type against WNV and can serve as a potential vaccine to prevent WNV infection...
Expression of the severe acute respiratory syndrome coronavirus 3a protein and the assembly of coronavirus-like particles in the baculovirus expression systemSehaam Khan
Collaborative Antiviral Research Group, Institute of Molecular and Cell Biology, Proteos, Singapore
Methods Mol Biol 379:35-50. 2007..Expression of viral proteins was confirmed by Western blot analysis and the formation of VLPs was studied by transmission electron microscopy...
Quantifying the specific binding between West Nile virus envelope domain III protein and the cellular receptor alphaVbeta3 integrinJason Wei Ming Lee
Flavivirology Laboratory, Department of Microbiology, National University of Singapore, Singapore 117597
J Biol Chem 281:1352-60. 2006..These experiments provided an insight to quantitate virus-receptor interaction. Force measurement using atomic force microscopy can serve to quantitatively analyze the effect of candidate drugs that modulate virus-host receptor affinity...
Interaction of West Nile virus with alpha v beta 3 integrin mediates virus entry into cellsJustin Jang Hann Chu
Flavivirology Laboratory, Department of Microbiology, 5 Science Drive 2, National University of Singapore, Singapore 117597
J Biol Chem 279:54533-41. 2004..The identification of alpha(v)beta(3) integrin as a receptor for WNV provides insight into virus-receptor interaction, hence creating opportunities in the development of anti-viral strategies against WNV infection...
Molecular mechanisms of West Nile virus pathogenesis in brain cellWee-Lee Koh
National University of Singapore, Singapore
Emerg Infect Dis 11:629-32. 2005..These changes may be useful as potential biomarkers and elucidate novel mechanisms behind the neuropathology of infection with this virus...
Analysis of self-association of West Nile virus capsid protein and the crucial role played by Trp 69 in homodimerizationRaghavan Bhuvanakantham
Flavivirology Laboratory, Department of Microbiology, 5 Science Drive 2, National University of Singapore, Singapore 117597, Singapore
Biochem Biophys Res Commun 329:246-55. 2005..The results of this study pinpoint a critical residue in the C protein that potentially plays a role in stabilizing the homotypic interaction...
