Research Topics
| Wen Tso LiuSummaryAffiliation: National University of Singapore Country: Singapore Publications
| Collaborators
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Detail Information
Publications
Optimization of an oligonucleotide microchip for microbial identification studies: a non-equilibrium dissociation approachW T Liu
Environmental Health Engineering Program, Department of Civil Engineering, Northwestern University, Evanston, IL 60208, USA
Environ Microbiol 3:619-29. 2001..The overall pattern of hybridization derived from this hierarchical probe set also provided a clear 'chip fingerprint' for each of these closely related Bacillus species...- Environmental microbiology-on-a-chip and its future impactsWen Tso Liu
Department of Civil Engineering, National University of Singapore, 117576, Singapore
Trends Biotechnol 23:174-9. 2005..In the long run, significant impacts are anticipated on environmental metagenomics and proteomics... - Emission characteristics of fluorescent labels with respect to temperature changes and subsequent effects on DNA microchip studiesWen Tso Liu
Department of Civil Engineering, National University of Singapore, Blk E1A, no 07 03, Engineering Drive 2, Singapore 117576
Appl Environ Microbiol 71:6453-7. 2005..To minimize the bias associated with the temperature-dependent emission of different fluorescent labels, a normalization step was proposed... - LabArray: real-time imaging and analytical tool for microarraysJohnson Kian-Kok Ng
Division of Bioengineering 10 Kent Ridge Crescent, Singapore 119260, Singapore
Bioinformatics 21:689-90. 2005..AVAILABILITY: LabArray is available at http://www.eng.nus.edu.sg/civil/Labarray/labarray.htm CONTACT: SUPPLEMENTARY INFORMATION: Screenshots and instructions for use are available at the above website... - Microfluidic device as a new platform for immunofluorescent detection of virusesWen Tso Liu
Division of Environmental Science and Engineering, National University of Singapore, Blk E1A, 07 03, Engineering Drive 2, Singapore 117576
Lab Chip 5:1327-30. 2005..25 h to <30 min, and the amount of antibody consumed was reduced by a factor of 14.3... - Kineosphaera limosa gen. nov., sp. nov., a novel Gram-positive polyhydroxyalkanoate-accumulating coccus isolated from activated sludgeWen Tso Liu
Department of Civil Engineering, National University of Singapore, Singapore
Int J Syst Evol Microbiol 52:1845-9. 2002..The name Kineosphaera limosa is proposed for strain Lpha5T (= JCM 11399T = DSM 14548T)... - Microbial community dynamics during start-up of acidogenic anaerobic reactorsWen Tso Liu
Department of Civil Engineering, Centre for Water Research, National University of Singapore, Singapore
Water Res 36:3203-10. 2002..Significant microbial population changes took place at the first 13 days for both reactors, but a longer period up to 71 days was required to establish a microbial community with a stable metabolic activity... - Characterization of microbial community in granular sludge treating brewery wastewaterWen Tso Liu
Graduate Institute of Environmental Engineering, National Central University, Taiwan
Water Res 36:1767-75. 2002..e., Desulfovibrio spp.), hydrogenotrophic Methanobacteriales and aceticlastic Methanosaeta were observed to presumably form a syntrophic association. Finally, the center core consisted of microcolonies of Methanosaeta cells... - Relative abundance of Bacteroides spp. in stools and wastewaters as determined by hierarchical oligonucleotide primer extensionPei Ying Hong
Division of Environmental Science and Engineering, National University of Singapore, E2 04 07, 1 Engineering Dr 2, Singapore 117576, Republic of Singapore
Appl Environ Microbiol 74:2882-93. 2008..predominant in fecal samples... - Microbial community structure in a thermophilic anaerobic hybrid reactor degrading terephthalateChia Lung Chen
Department of Civil Engineering, National University of Singapore, Blk E1A, 07 03, Engineering Drive 2, 117576, Singapore
Microbiology 150:3429-40. 2004..These newly emerged populations were probably also capable of degrading terephthalate in the hybrid system, but were out-competed by those bacterial populations before perturbations... - Quantitative multiplexing analysis of PCR-amplified ribosomal RNA genes by hierarchical oligonucleotide primer extension reactionJer Horng Wu
Division of Environmental Science and Engineering, National University of Singapore, Singapore 117576
Nucleic Acids Res 35:e82. 2007..Single mismatch discrimination with detection sensitivity of a target down to 0.01-0.05% of total DNA template is achieved... - A high-throughput and quantitative hierarchical oligonucleotide primer extension (HOPE)-based approach to identify sources of faecal contamination in water bodiesPei Ying Hong
Division of Environmental Science and Engineering, National University of Singapore, Singapore 117576
Environ Microbiol 11:1672-81. 2009..Overall, this study demonstrates HOPE as a quantitative and high-throughput method that can identify sources of faecal contamination... - Effects of target length on the hybridization efficiency and specificity of rRNA-based oligonucleotide microarraysWen Tso Liu
Division of Environmental Science and Engineering, National University of Singapore, Block E2, no 04 07, 1 Engineering Drive 2, Singapore 117576
Appl Environ Microbiol 73:73-82. 2007..Such an understanding is important to the application of DNA microarray technology in microbial community studies... - Identification and occurrence of tetrad-forming Alphaproteobacteria in anaerobic-aerobic activated sludge processesMan-Tak Wong
Environmental Science and Engineering Program and Department of Civil Engineering, National University of Singapore, Singapore 117576
Microbiology 150:3741-8. 2004..However, these 'Defluvicoccus'-related TFOs were only seen in low abundance in 12 different EBPR and non-EBPR systems, suggesting that they were not the key populations responsible for the deterioration of full-scale EBPR processes... - Pyrosequencing analysis of bacterial biofilm communities in water meters of a drinking water distribution systemPei Ying Hong
Division of Environmental Science and Engineering, National University of Singapore, Singapore, Republic of Singapore
Appl Environ Microbiol 76:5631-5. 2010..Differences in bacterial diversity and composition were observed. A better understanding of the bacterial ecology of drinking water biofilms will allow for effective management of water quality in distribution systems... - Ecophysiology of Defluviicoccus-related tetrad-forming organisms in an anaerobic-aerobic activated sludge processMan Tak Wong
Division of Environmental Science and Engineering, National University of Singapore, Singapore 117576
Environ Microbiol 9:1485-96. 2007.... - Identification of important microbial populations in the mesophilic and thermophilic phenol-degrading methanogenic consortiaChia Lung Chen
Department of Civil Engineering, National University of Singapore, Singapore
Water Res 42:1963-76. 2008..FISH further revealed that member of the Deltaproteobacteria group TA represented more than 68.3% of EUBmix FISH-detectable bacterial cell area in a full-scale mesophilic bioreactor treating phenol-containing wastewaters... - Comparative analysis of fecal microbiota in infants with and without eczemaPei Ying Hong
Division of Environmental Science and Engineering, National University of Singapore, Singapore
PLoS ONE 5:e9964. 2010.... - Recent advances in molecular techniques for the detection of phylogenetic markers and functional genes in microbial communitiesStanley C K Lau
Division of Environmental Science and Engineering, National University of Singapore, Singapore, Singapore
FEMS Microbiol Lett 275:183-90. 2007..Considerable efforts have been made to enhance the capability of molecular techniques. Here an update of the recent developments in molecular techniques for environmental microbiology is provided... - Spatially addressable bead-based biosensor for rapid detection of beta-thalassemia mutationsJohnson Kian Kok Ng
Department of Paediatrics, Yong Loo Lin School of Medicine, National University of Singapore, Singapore
Anal Chim Acta 658:193-6. 2010..This device successfully detected all six common beta-globin gene mutations within 30 min. The number of targeted mutations on this chip can be easily expandable through the introduction of additional probe sets... - Rapid discrimination of single-nucleotide mismatches using a microfluidic device with monolayered beadsJohnson Kian Kok Ng
Division of Environmental Science and Engineering, National University of Singapore, EA 07 23, 9 Engineering Drive 1, Singapore 117576, Singapore
Anal Chim Acta 582:295-303. 2007..The device can easily be modified to integrate multiplexed detection, making high-throughput SNP detection possible... - Nanoparticles and their biological and environmental applicationsWen Tso Liu
Division of Environmental Science and Engineering, National University of Singapore, E2 4 7, Engineering Drive 2, Singapore 117576, Singapore
J Biosci Bioeng 102:1-7. 2006.... - In situ identification and characterization of the microbial community structure of full-scale enhanced biological phosphorous removal plants in JapanMan-Tak Wong
Department of Civil Engineering, National University of Singapore, Blk E1A, 07-03, Engineering Drive 2, Singapore 117576, Singapore
Water Res 39:2901-14. 2005..These non-Rhodocyclus-related PAOs included populations from other bacterial divisions and members of the Betaproteobacteria other than those in Rhodocyclus-related group... - Cell loss in integrated microfluidic deviceLiang Zhu
Institute of Microelectronics, Agency for Science, Technology and Research, 11 Science Park Road, Singapore Science Park II, Singapore, Singapore, 117685
Biomed Microdevices 9:745-50. 2007..8+/-5.5% and 41.3+/-3.6% without hydrodynamic focusing, respectively, to 90.8+/-5.8%, 89.8+/-16.6% and 77.0+/-9.2% with hydrodynamic focusing... - Biological filtration limits carbon availability and affects downstream biofilm formation and community structureChee Meng Pang
Division of Environmental Science and Engineering, National University of Singapore, Block E2, No. 04-07, 1 Engineering Drive 2, Singapore 117576
Appl Environ Microbiol 72:5702-12. 2006..g., Aquabacterium, Caulobacter, and Legionella) were preferentially selected in the BF biofilm, suggesting that carbon limitation strategies may not achieve adequate biofouling control in the long run... - Hierarchical oligonucleotide primer extension as a time- and cost-effective approach for quantitative determination of Bifidobacterium spp. in infant fecesPei Ying Hong
Division of Environmental Science and Engineering, National University of Singapore, Singapore 117576, USA
Appl Environ Microbiol 75:2573-6. 2009..783; P value, <0.066). This good comparability supports HOPE as a method to achieve high-throughput quantitative determination of bacterial targets in a time- and cost-effective manner... - Determination of virus abundance, diversity and distribution in a municipal wastewater treatment plantQinglong Wu
Division of Environmental Sciences and Engineering, Faculty of Engineering, National University of Singapore, 9 Engineering Drive 1, Singapore 117576, Singapore
Water Res 43:1101-9. 2009..Collectively, our study suggested that indigenous viruses are abundant and dynamic in the municipal wastewater treatment system and may play an important role in functioning of the system... - Characterization of active microbes in a full-scale anaerobic fluidized bed reactor treating phenolic wastewaterChia Lung Chen
Department of Civil Engineering, National University of Singapore
Microbes Environ 24:144-53. 2009..These findings greatly improve our understanding of the diversity and distribution of microbial populations in a full-scale mesophilic bioreactor treating an actual phenol-containing waste stream... - A spatially addressable bead-based biosensor for simple and rapid DNA detectionJohnson K Ng
Division of Environmental Science and Engineering, National University of Singapore, 21 Lower Kent Ridge Road, Singapore 119077, Singapore
Biosens Bioelectron 23:803-10. 2008.... - Evaluating single-base-pair discriminating capability of planar oligonucleotide microchips using a non-equilibrium dissociation approachEmily Sze Ying Li
Environmental Science and Engineering Program, and Department of Civil Engineering, National University of Singapore, Singapore 117576
Environ Microbiol 6:1197-202. 2004..The reusability potential of the planar microchip was further demonstrated... - Diversity and distribution of a deeply branched novel proteobacterial group found in anaerobic-aerobic activated sludge processesYunhong Kong
Department of Civil Engineering, National University of Singapore, Singapore 117576
Environ Microbiol 4:753-7. 2002..Variation of predominance was also observed among those subgroups in systems showing deteriorated or effective EBPR activity... - Community structure analysis of reverse osmosis membrane biofilms and the significance of Rhizobiales bacteria in biofoulingChee Meng Pang
Department of Civil Engineering and Division of Environmental Science and Engineering, National University of Singapore, Block E2 04 07, 1 Engineering Drive 2, Singapore 117576, Singapore
Environ Sci Technol 41:4728-34. 2007..These findings suggest that Rhizobiales organisms are ecologically significant in membrane biofilm communities under both aerobic and anoxic conditions and may be responsible for biofouling in membrane separation systems... - Biofilm formation characteristics of bacterial isolates retrieved from a reverse osmosis membraneChee Meng Pang
Department of Civil Engineering, National University of Singapore, Block E2, 04-07, 1 Engineering Drive 2, Singapore 117576
Environ Sci Technol 39:7541-50. 2005..These results suggested that RO2 could colonize RO membranes effectively and could be a potential fouling organism in RO membranes for freshwater purification... - Quantum dots as a novel immunofluorescent detection system for Cryptosporidium parvum and Giardia lambliaLiang Zhu
Departments of Civil Engineering, National University of Singapore, Singapore
Appl Environ Microbiol 70:597-8. 2004..This novel fluorescence system exhibited superior photostability, gave 1.5- to 9-fold-higher signal-to-noise ratios than traditional organic dyes in detecting C. parvum, and allowed dual-color detection for C. parvum and G. lamblia... - Rapid lab-on-a-chip profiling of human gut bacteriaJoakim Bjerketorp
Department of Microbiology, Swedish University of Agricultural Sciences, Uppsala, Sweden
J Microbiol Methods 72:82-90. 2008..Although the resolution of the method needs improvement, this study represents the first step towards development of a diagnostic LOC for profiling gut bacterial communities... - Tetrasphaera elongata sp. nov., a polyphosphate-accumulating bacterium isolated from activated sludgeSatoshi Hanada
Research Institute of Biological Resources, National Institute of Advanced Industrial Science and Technology, Tsukuba, Japan
Int J Syst Evol Microbiol 52:883-7. 2002..nov. is proposed. The type strain is strain Lp2T (= JCM 11141T = DSM 14184T)... - Enhanced microfiltration devices configured with hydrodynamic trapping and a rain drop bypass filtering architecture for microbial cells detectionChristophe Lay
Institute of Microelectronics, A STAR Agency for Science, Technology and Research, Singapore
Lab Chip 8:830-3. 2008.... - Saliva-based diagnostics using 16S rRNA microarrays and microfluidicsE Michelle Starke
Civil and Environmental Engineering, University of Washington, Seattle, Washington 98195 2700, USA
Ann N Y Acad Sci 1098:345-61. 2007..This article discusses recent improvements of our system in reducing diffusional constraints in order to provide more rapid and accurate measurements of the microbial composition of saliva...