Wen Tso Liu

Summary

Affiliation: National University of Singapore
Country: Singapore

Publications

  1. ncbi request reprint Optimization of an oligonucleotide microchip for microbial identification studies: a non-equilibrium dissociation approach
    W T Liu
    Environmental Health Engineering Program, Department of Civil Engineering, Northwestern University, Evanston, IL 60208, USA
    Environ Microbiol 3:619-29. 2001
  2. ncbi request reprint Characterization of microbial community in granular sludge treating brewery wastewater
    Wen Tso Liu
    Graduate Institute of Environmental Engineering, National Central University, Taiwan
    Water Res 36:1767-75. 2002
  3. ncbi request reprint Environmental microbiology-on-a-chip and its future impacts
    Wen Tso Liu
    Department of Civil Engineering, National University of Singapore, 117576, Singapore
    Trends Biotechnol 23:174-9. 2005
  4. ncbi request reprint Microbial community dynamics during start-up of acidogenic anaerobic reactors
    Wen Tso Liu
    Department of Civil Engineering, Centre for Water Research, National University of Singapore, Singapore
    Water Res 36:3203-10. 2002
  5. ncbi request reprint Kineosphaera limosa gen. nov., sp. nov., a novel Gram-positive polyhydroxyalkanoate-accumulating coccus isolated from activated sludge
    Wen Tso Liu
    Department of Civil Engineering, National University of Singapore, Singapore
    Int J Syst Evol Microbiol 52:1845-9. 2002
  6. ncbi request reprint LabArray: real-time imaging and analytical tool for microarrays
    Johnson Kian Kok Ng
    Division of Bioengineering 10 Kent Ridge Crescent, Singapore 119260, Singapore
    Bioinformatics 21:689-90. 2005
  7. pmc Emission characteristics of fluorescent labels with respect to temperature changes and subsequent effects on DNA microchip studies
    Wen Tso Liu
    Department of Civil Engineering, National University of Singapore, Blk E1A, no 07 03, Engineering Drive 2, Singapore 117576
    Appl Environ Microbiol 71:6453-7. 2005
  8. ncbi request reprint Microfluidic device as a new platform for immunofluorescent detection of viruses
    Wen Tso Liu
    Division of Environmental Science and Engineering, National University of Singapore, Blk E1A, 07 03, Engineering Drive 2, Singapore 117576
    Lab Chip 5:1327-30. 2005
  9. ncbi request reprint Identification and occurrence of tetrad-forming Alphaproteobacteria in anaerobic-aerobic activated sludge processes
    Man Tak Wong
    Environmental Science and Engineering Program and Department of Civil Engineering, National University of Singapore, Singapore 117576
    Microbiology 150:3741-8. 2004
  10. doi request reprint Diversity of bacterioplankton in contrasting Tibetan lakes revealed by high-density microarray and clone library analysis
    Rui Zhang
    Division of Environmental Science and Engineering, National University of Singapore, Singapore State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen, China
    FEMS Microbiol Ecol 86:277-87. 2013

Collaborators

Detail Information

Publications41

  1. ncbi request reprint Optimization of an oligonucleotide microchip for microbial identification studies: a non-equilibrium dissociation approach
    W T Liu
    Environmental Health Engineering Program, Department of Civil Engineering, Northwestern University, Evanston, IL 60208, USA
    Environ Microbiol 3:619-29. 2001
    ..The overall pattern of hybridization derived from this hierarchical probe set also provided a clear 'chip fingerprint' for each of these closely related Bacillus species...
  2. ncbi request reprint Characterization of microbial community in granular sludge treating brewery wastewater
    Wen Tso Liu
    Graduate Institute of Environmental Engineering, National Central University, Taiwan
    Water Res 36:1767-75. 2002
    ..e., Desulfovibrio spp.), hydrogenotrophic Methanobacteriales and aceticlastic Methanosaeta were observed to presumably form a syntrophic association. Finally, the center core consisted of microcolonies of Methanosaeta cells...
  3. ncbi request reprint Environmental microbiology-on-a-chip and its future impacts
    Wen Tso Liu
    Department of Civil Engineering, National University of Singapore, 117576, Singapore
    Trends Biotechnol 23:174-9. 2005
    ..In the long run, significant impacts are anticipated on environmental metagenomics and proteomics...
  4. ncbi request reprint Microbial community dynamics during start-up of acidogenic anaerobic reactors
    Wen Tso Liu
    Department of Civil Engineering, Centre for Water Research, National University of Singapore, Singapore
    Water Res 36:3203-10. 2002
    ..Significant microbial population changes took place at the first 13 days for both reactors, but a longer period up to 71 days was required to establish a microbial community with a stable metabolic activity...
  5. ncbi request reprint Kineosphaera limosa gen. nov., sp. nov., a novel Gram-positive polyhydroxyalkanoate-accumulating coccus isolated from activated sludge
    Wen Tso Liu
    Department of Civil Engineering, National University of Singapore, Singapore
    Int J Syst Evol Microbiol 52:1845-9. 2002
    ..The name Kineosphaera limosa is proposed for strain Lpha5T (= JCM 11399T = DSM 14548T)...
  6. ncbi request reprint LabArray: real-time imaging and analytical tool for microarrays
    Johnson Kian Kok Ng
    Division of Bioengineering 10 Kent Ridge Crescent, Singapore 119260, Singapore
    Bioinformatics 21:689-90. 2005
    ..AVAILABILITY: LabArray is available at http://www.eng.nus.edu.sg/civil/Labarray/labarray.htm CONTACT: cveliuwt@nus.edu.sg SUPPLEMENTARY INFORMATION: Screenshots and instructions for use are available at the above website...
  7. pmc Emission characteristics of fluorescent labels with respect to temperature changes and subsequent effects on DNA microchip studies
    Wen Tso Liu
    Department of Civil Engineering, National University of Singapore, Blk E1A, no 07 03, Engineering Drive 2, Singapore 117576
    Appl Environ Microbiol 71:6453-7. 2005
    ..To minimize the bias associated with the temperature-dependent emission of different fluorescent labels, a normalization step was proposed...
  8. ncbi request reprint Microfluidic device as a new platform for immunofluorescent detection of viruses
    Wen Tso Liu
    Division of Environmental Science and Engineering, National University of Singapore, Blk E1A, 07 03, Engineering Drive 2, Singapore 117576
    Lab Chip 5:1327-30. 2005
    ..25 h to <30 min, and the amount of antibody consumed was reduced by a factor of 14.3...
  9. ncbi request reprint Identification and occurrence of tetrad-forming Alphaproteobacteria in anaerobic-aerobic activated sludge processes
    Man Tak Wong
    Environmental Science and Engineering Program and Department of Civil Engineering, National University of Singapore, Singapore 117576
    Microbiology 150:3741-8. 2004
    ..However, these 'Defluvicoccus'-related TFOs were only seen in low abundance in 12 different EBPR and non-EBPR systems, suggesting that they were not the key populations responsible for the deterioration of full-scale EBPR processes...
  10. doi request reprint Diversity of bacterioplankton in contrasting Tibetan lakes revealed by high-density microarray and clone library analysis
    Rui Zhang
    Division of Environmental Science and Engineering, National University of Singapore, Singapore State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen, China
    FEMS Microbiol Ecol 86:277-87. 2013
    ..Our study suggested high microbial cosmopolitanism and high endemicity observed at higher and lower phylogenetic levels, respectively. ..
  11. pmc Biological filtration limits carbon availability and affects downstream biofilm formation and community structure
    Chee Meng Pang
    Division of Environmental Science and Engineering, National University of Singapore, Block E2, no 04 07, 1 Engineering Drive 2, Singapore 117576
    Appl Environ Microbiol 72:5702-12. 2006
    ..g., Aquabacterium, Caulobacter, and Legionella) were preferentially selected in the BF biofilm, suggesting that carbon limitation strategies may not achieve adequate biofouling control in the long run...
  12. pmc Relative abundance of Bacteroides spp. in stools and wastewaters as determined by hierarchical oligonucleotide primer extension
    Pei Ying Hong
    Division of Environmental Science and Engineering, National University of Singapore, E2 04 07, 1 Engineering Dr 2, Singapore 117576, Republic of Singapore
    Appl Environ Microbiol 74:2882-93. 2008
    ..predominant in fecal samples...
  13. ncbi request reprint Filter-based microfluidic device as a platform for immunofluorescent assay of microbial cells
    Liang Zhu
    Department of Civil Engineering, National University of Singapore, Singapore 117576, Republic of Singapore
    Lab Chip 4:337-41. 2004
    ....
  14. ncbi request reprint In situ identification and characterization of the microbial community structure of full-scale enhanced biological phosphorous removal plants in Japan
    Man Tak Wong
    Department of Civil Engineering, National University of Singapore, Blk E1A, 07 03, Engineering Drive 2, Singapore 117576, Singapore
    Water Res 39:2901-14. 2005
    ..These non-Rhodocyclus-related PAOs included populations from other bacterial divisions and members of the Betaproteobacteria other than those in Rhodocyclus-related group...
  15. ncbi request reprint Evaluating single-base-pair discriminating capability of planar oligonucleotide microchips using a non-equilibrium dissociation approach
    Emily Sze Ying Li
    Environmental Science and Engineering Program, and Department of Civil Engineering, National University of Singapore, Singapore 117576
    Environ Microbiol 6:1197-202. 2004
    ..The reusability potential of the planar microchip was further demonstrated...
  16. ncbi request reprint Microbial community structure in a thermophilic anaerobic hybrid reactor degrading terephthalate
    Chia Lung Chen
    Department of Civil Engineering, National University of Singapore, Blk E1A, 07 03, Engineering Drive 2, 117576, Singapore
    Microbiology 150:3429-40. 2004
    ..These newly emerged populations were probably also capable of degrading terephthalate in the hybrid system, but were out-competed by those bacterial populations before perturbations...
  17. doi request reprint A high-throughput and quantitative hierarchical oligonucleotide primer extension (HOPE)-based approach to identify sources of faecal contamination in water bodies
    Pei Ying Hong
    Division of Environmental Science and Engineering, National University of Singapore, Singapore 117576
    Environ Microbiol 11:1672-81. 2009
    ..Overall, this study demonstrates HOPE as a quantitative and high-throughput method that can identify sources of faecal contamination...
  18. pmc Quantitative multiplexing analysis of PCR-amplified ribosomal RNA genes by hierarchical oligonucleotide primer extension reaction
    Jer Horng Wu
    Division of Environmental Science and Engineering, National University of Singapore, Singapore 117576
    Nucleic Acids Res 35:e82. 2007
    ..Single mismatch discrimination with detection sensitivity of a target down to 0.01-0.05% of total DNA template is achieved...
  19. ncbi request reprint Ecophysiology of Defluviicoccus-related tetrad-forming organisms in an anaerobic-aerobic activated sludge process
    Man Tak Wong
    Division of Environmental Science and Engineering, National University of Singapore, Singapore 117576
    Environ Microbiol 9:1485-96. 2007
    ....
  20. pmc Pyrosequencing analysis of bacterial biofilm communities in water meters of a drinking water distribution system
    Pei Ying Hong
    Division of Environmental Science and Engineering, National University of Singapore, Singapore, Republic of Singapore
    Appl Environ Microbiol 76:5631-5. 2010
    ..Differences in bacterial diversity and composition were observed. A better understanding of the bacterial ecology of drinking water biofilms will allow for effective management of water quality in distribution systems...
  21. pmc Effects of target length on the hybridization efficiency and specificity of rRNA-based oligonucleotide microarrays
    Wen Tso Liu
    Division of Environmental Science and Engineering, National University of Singapore, Block E2, no 04 07, 1 Engineering Drive 2, Singapore 117576
    Appl Environ Microbiol 73:73-82. 2007
    ..Such an understanding is important to the application of DNA microarray technology in microbial community studies...
  22. ncbi request reprint Diversity and distribution of a deeply branched novel proteobacterial group found in anaerobic-aerobic activated sludge processes
    Yunhong Kong
    Department of Civil Engineering, National University of Singapore, Singapore 117576
    Environ Microbiol 4:753-7. 2002
    ..Variation of predominance was also observed among those subgroups in systems showing deteriorated or effective EBPR activity...
  23. pmc Comparative analysis of fecal microbiota in infants with and without eczema
    Pei Ying Hong
    Division of Environmental Science and Engineering, National University of Singapore, Singapore
    PLoS ONE 5:e9964. 2010
    ....
  24. doi request reprint Biogeography of bacterioplankton in the tropical seawaters of Singapore
    Stanley C K Lau
    Division of Environmental Science and Engineering, National University of Singapore, Singapore
    FEMS Microbiol Ecol 84:259-69. 2013
    ..Overall, these results provide valuable insights into the diversity of bacterioplankton communities in Singapore seas and the possible influences of hydrological conditions and anthropogenic activities on the dynamics of the communities...
  25. pmc Quantum dots as a novel immunofluorescent detection system for Cryptosporidium parvum and Giardia lamblia
    Liang Zhu
    Departments of Civil Engineering, National University of Singapore, Singapore
    Appl Environ Microbiol 70:597-8. 2004
    ..This novel fluorescence system exhibited superior photostability, gave 1.5- to 9-fold-higher signal-to-noise ratios than traditional organic dyes in detecting C. parvum, and allowed dual-color detection for C. parvum and G. lamblia...
  26. ncbi request reprint Recent advances in molecular techniques for the detection of phylogenetic markers and functional genes in microbial communities
    Stanley C K Lau
    Division of Environmental Science and Engineering, National University of Singapore, Singapore, Singapore
    FEMS Microbiol Lett 275:183-90. 2007
    ..Considerable efforts have been made to enhance the capability of molecular techniques. Here an update of the recent developments in molecular techniques for environmental microbiology is provided...
  27. ncbi request reprint Biofilm formation characteristics of bacterial isolates retrieved from a reverse osmosis membrane
    Chee Meng Pang
    Department of Civil Engineering, National University of Singapore, Block E2, 04 07, 1 Engineering Drive 2, Singapore 117576
    Environ Sci Technol 39:7541-50. 2005
    ..These results suggested that RO2 could colonize RO membranes effectively and could be a potential fouling organism in RO membranes for freshwater purification...
  28. doi request reprint Identification of important microbial populations in the mesophilic and thermophilic phenol-degrading methanogenic consortia
    Chia Lung Chen
    Department of Civil Engineering, National University of Singapore, Singapore
    Water Res 42:1963-76. 2008
    ..FISH further revealed that member of the Deltaproteobacteria group TA represented more than 68.3% of EUBmix FISH-detectable bacterial cell area in a full-scale mesophilic bioreactor treating phenol-containing wastewaters...
  29. doi request reprint Spatially addressable bead-based biosensor for rapid detection of beta-thalassemia mutations
    Johnson Kian Kok Ng
    Department of Paediatrics, Yong Loo Lin School of Medicine, National University of Singapore, Singapore
    Anal Chim Acta 658:193-6. 2010
    ..This device successfully detected all six common beta-globin gene mutations within 30 min. The number of targeted mutations on this chip can be easily expandable through the introduction of additional probe sets...
  30. ncbi request reprint Nanoparticles and their biological and environmental applications
    Wen Tso Liu
    Division of Environmental Science and Engineering, National University of Singapore, E2 4 7, Engineering Drive 2, Singapore 117576, Singapore
    J Biosci Bioeng 102:1-7. 2006
    ....
  31. ncbi request reprint Cell loss in integrated microfluidic device
    Liang Zhu
    Institute of Microelectronics, Agency for Science, Technology and Research, 11 Science Park Road, Singapore Science Park II, Singapore, Singapore, 117685
    Biomed Microdevices 9:745-50. 2007
    ..8+/-5.5% and 41.3+/-3.6% without hydrodynamic focusing, respectively, to 90.8+/-5.8%, 89.8+/-16.6% and 77.0+/-9.2% with hydrodynamic focusing...
  32. ncbi request reprint Rapid discrimination of single-nucleotide mismatches using a microfluidic device with monolayered beads
    Johnson Kian Kok Ng
    Division of Environmental Science and Engineering, National University of Singapore, EA 07 23, 9 Engineering Drive 1, Singapore 117576, Singapore
    Anal Chim Acta 582:295-303. 2007
    ..The device can easily be modified to integrate multiplexed detection, making high-throughput SNP detection possible...
  33. ncbi request reprint Characterization of active microbes in a full-scale anaerobic fluidized bed reactor treating phenolic wastewater
    Chia Lung Chen
    Department of Civil Engineering, National University of Singapore
    Microbes Environ 24:144-53. 2009
    ..These findings greatly improve our understanding of the diversity and distribution of microbial populations in a full-scale mesophilic bioreactor treating an actual phenol-containing waste stream...
  34. pmc Hierarchical oligonucleotide primer extension as a time- and cost-effective approach for quantitative determination of Bifidobacterium spp. in infant feces
    Pei Ying Hong
    Division of Environmental Science and Engineering, National University of Singapore, Singapore 117576, USA
    Appl Environ Microbiol 75:2573-6. 2009
    ..783; P value, <0.066). This good comparability supports HOPE as a method to achieve high-throughput quantitative determination of bacterial targets in a time- and cost-effective manner...
  35. ncbi request reprint A spatially addressable bead-based biosensor for simple and rapid DNA detection
    Johnson K Ng
    Division of Environmental Science and Engineering, National University of Singapore, 21 Lower Kent Ridge Road, Singapore 119077, Singapore
    Biosens Bioelectron 23:803-10. 2008
    ....
  36. ncbi request reprint Determination of virus abundance, diversity and distribution in a municipal wastewater treatment plant
    Qinglong Wu
    Division of Environmental Sciences and Engineering, Faculty of Engineering, National University of Singapore, 9 Engineering Drive 1, Singapore 117576, Singapore
    Water Res 43:1101-9. 2009
    ..Collectively, our study suggested that indigenous viruses are abundant and dynamic in the municipal wastewater treatment system and may play an important role in functioning of the system...
  37. ncbi request reprint Community structure analysis of reverse osmosis membrane biofilms and the significance of Rhizobiales bacteria in biofouling
    Chee Meng Pang
    Department of Civil Engineering and Division of Environmental Science and Engineering, National University of Singapore, Block E2 04 07, 1 Engineering Drive 2, Singapore 117576, Singapore
    Environ Sci Technol 41:4728-34. 2007
    ..These findings suggest that Rhizobiales organisms are ecologically significant in membrane biofilm communities under both aerobic and anoxic conditions and may be responsible for biofouling in membrane separation systems...
  38. ncbi request reprint Tetrasphaera elongata sp. nov., a polyphosphate-accumulating bacterium isolated from activated sludge
    Satoshi Hanada
    Research Institute of Biological Resources, National Institute of Advanced Industrial Science and Technology, Tsukuba, Japan
    Int J Syst Evol Microbiol 52:883-7. 2002
    ..nov. is proposed. The type strain is strain Lp2T (= JCM 11141T = DSM 14184T)...
  39. ncbi request reprint Rapid lab-on-a-chip profiling of human gut bacteria
    Joakim Bjerketorp
    Department of Microbiology, Swedish University of Agricultural Sciences, Uppsala, Sweden
    J Microbiol Methods 72:82-90. 2008
    ..Although the resolution of the method needs improvement, this study represents the first step towards development of a diagnostic LOC for profiling gut bacterial communities...
  40. ncbi request reprint Saliva-based diagnostics using 16S rRNA microarrays and microfluidics
    E Michelle Starke
    Civil and Environmental Engineering, University of Washington, Seattle, Washington 98195 2700, USA
    Ann N Y Acad Sci 1098:345-61. 2007
    ..This article discusses recent improvements of our system in reducing diffusional constraints in order to provide more rapid and accurate measurements of the microbial composition of saliva...
  41. doi request reprint Enhanced microfiltration devices configured with hydrodynamic trapping and a rain drop bypass filtering architecture for microbial cells detection
    Christophe Lay
    Institute of Microelectronics, A STAR Agency for Science, Technology and Research, Singapore
    Lab Chip 8:830-3. 2008
    ....