Zhiyuan Gong


Affiliation: National University of Singapore
Country: Singapore


  1. Li H, Lu J, Huo X, Li Y, Li Z, Gong Z. Effects of sex hormones on liver tumor progression and regression in Myc/xmrk double oncogene transgenic zebrafish. Gen Comp Endocrinol. 2019;: pubmed publisher
    ..Furthermore, androgen promoted cell proliferation while estrogen inhibited it. Overall, the present study suggested that sex hormones affected liver tumor progression and regression in the Myc/xmrk transgenic zebrafish. ..
  2. Yang Q, Salim L, Yan C, Gong Z. Rapid Analysis of Effects of Environmental Toxicants on Tumorigenesis and Inflammation Using a Transgenic Zebrafish Model for Liver Cancer. Mar Biotechnol (NY). 2019;: pubmed publisher
    ..Thus, our study established a screening system to rapidly assess the effects of candidate chemicals on liver tumorigenesis and inflammation. ..
  3. Yang Q, Yan C, Wang X, Gong Z. Leptin induces muscle wasting in a zebrafish kras-driven hepatocellular carcinoma (HCC) model. Dis Model Mech. 2019;12: pubmed publisher
    ..Chemical inhibitors targeting leptin signaling also alleviated the muscle-wasting phenotype, indicating that leptin signaling may be a new therapeutic target for cancer patients with muscle wasting. ..
  4. Huo X, Li H, Li Z, Yan C, Agrawal I, Mathavan S, et al. Transcriptomic profiles of tumor-associated neutrophils reveal prominent roles in enhancing angiogenesis in liver tumorigenesis in zebrafish. Sci Rep. 2019;9:1509 pubmed publisher
    ..Collectively, our study has indicated some molecular mechanisms of the pro-tumoral roles of TANs in hepatocarcinogenesis, including weakened immune clearance against tumor cells and enhanced function in angiogenesis. ..
  5. Huo X, Li H, Li Z, Yan C, Mathavan S, Liu J, et al. Transcriptomic analyses of oncogenic hepatocytes reveal common and different molecular pathways of hepatocarcinogenesis in different developmental stages and genders in krasG12V transgenic zebrafish. Biochem Biophys Res Commun. 2019;510:558-564 pubmed publisher
    ..Finally, the two adult groups were more similar to each other than to the larval group, indicating an overwhelming effect of development over the gender. ..
  6. Yan C, Yang Q, Gong Z. Transgenic expression of tgfb1a induces hepatic inflammation, fibrosis and metastasis in zebrafish. Biochem Biophys Res Commun. 2018;: pubmed publisher
    ..Furthermore, we found high expression of EMT markers in late liver tumorigenesis, indicating a tumor metastasis potential. These observations are generally consistent with the molecular mechanisms of hepatocarcinogenesis in human. ..
  7. Lu J, Raghuram D, Fong P, Gong Z. Inducible Intestine-Specific Expression of krasV12 Triggers Intestinal Tumorigenesis In Transgenic Zebrafish. Neoplasia. 2018;20:1187-1197 pubmed publisher
    ..Thus, our transgenic zebrafish may provide a valuable in vivo platform that can be used to investigate tumor initiation and anticancer drugs for gastrointestinal cancers. ..
  8. Yang Q, Yan C, Gong Z. Interaction of hepatic stellate cells with neutrophils and macrophages in the liver following oncogenic kras activation in transgenic zebrafish. Sci Rep. 2018;8:8495 pubmed publisher
    ..Since the microenvironment crosstalk plays a vital role in manipulation of liver carcinogenesis, the underlying mechanism may provide potential therapeutic targets for liver diseases. ..
  9. Lee Y, Li C, Lam S, Gong Z, Lin Q. Proteomic Analysis of Zebrafish (Danio rerio) After Chemical Exposure. Methods Mol Biol. 2018;1797:443-459 pubmed publisher

More Information


  1. Xu H, Zhang X, Li H, Li C, Huo X, Hou L, et al. Immune response induced by major environmental pollutants through altering neutrophils in zebrafish larvae. Aquat Toxicol. 2018;201:99-108 pubmed publisher
    ..This study has also demonstrated that Tg(lyz:DsRed2) transgenic zebrafish is an excellent tool for screening environmental chemicals with potential inflammatory effects through FACS-facilitated neutrophil counting. ..
  2. Xu H, Li C, Li Y, Ng G, Liu C, Zhang X, et al. Generation of Tg(cyp1a:gfp) Transgenic Zebrafish for Development of a Convenient and Sensitive In Vivo Assay for Aryl Hydrocarbon Receptor Activity. Mar Biotechnol (NY). 2015;17:831-40 pubmed publisher
  3. Yan C, Yang Q, Gong Z. Activation of Hepatic Stellate Cells During Liver Carcinogenesis Requires Fibrinogen/Integrin αvβ5 in Zebrafish. Neoplasia. 2018;20:533-542 pubmed publisher
    ..Thus, we concluded a novel interaction between oncogenic hepatocytes and HSCs through the fibrinogen related pathway in both the zebrafish HCC model and human liver disease samples. ..
  4. Huang X, Agrawal I, Li Z, Zheng W, Lin Q, Gong Z. Transcriptomic Analyses in Zebrafish Cancer Models for Global Gene Expression and Pathway Discovery. Adv Exp Med Biol. 2016;916:147-68 pubmed publisher
    ..In the future, other genomic tools, such as epigenetic, proteomic and metabolomic tools will likely be incorporated into zebrafish cancer studies, further refining our understanding of cancer. ..
  5. Yan C, Yang Q, Gong Z. Tumor-Associated Neutrophils and Macrophages Promote Gender Disparity in Hepatocellular Carcinoma in Zebrafish. Cancer Res. 2017;77:1395-1407 pubmed publisher
    ..These results identify increased cortisol production and TAN/TAM infiltration as primary factors in the gender disparity of HCC development in both fish and human. Cancer Res; 77(6); 1395-407. ©2017 AACR. ..
  6. Wang Z, Du J, Lam S, Mathavan S, Matsudaira P, Gong Z. Morphological and molecular evidence for functional organization along the rostrocaudal axis of the adult zebrafish intestine. BMC Genomics. 2010;11:392 pubmed publisher
    ..Evolutionary lack of stomach, crypts, Paneth cells and submucosal glands has shaped the zebrafish intestine into a simpler but unique organ in vertebrate intestinal biology. ..
  7. Ung C, Lam S, Hlaing M, Winata C, Korzh S, Mathavan S, et al. Mercury-induced hepatotoxicity in zebrafish: in vivo mechanistic insights from transcriptome analysis, phenotype anchoring and targeted gene expression validation. BMC Genomics. 2010;11:212 pubmed publisher
    ..The molecular conservation of mercury-induced hepatotoxicity between zebrafish and human cell line reveals the feasibility of using zebrafish to model molecular toxicity in human for toxicant risk assessments. ..
  8. Mirbahai L, Williams T, Zhan H, Gong Z, Chipman J. Comprehensive profiling of zebrafish hepatic proximal promoter CpG island methylation and its modification during chemical carcinogenesis. BMC Genomics. 2011;12:3 pubmed publisher
    ..These findings increase the confidence in the use of zebrafish as a model for human cancer in addition to providing the first comprehensive mapping of DNA methylation in the normal adult zebrafish liver. ..
  9. Li Z, Korzh V, Gong Z. Localized rbp4 expression in the yolk syncytial layer plays a role in yolk cell extension and early liver development. BMC Dev Biol. 2007;7:117 pubmed
    ..YSL-expressed Rbp4 plays a role in formation of both yolk extension and liver bud, the latter may also require migration of liver progenitor cells. ..
  10. Winata C, Korzh S, Kondrychyn I, Korzh V, Gong Z. The role of vasculature and blood circulation in zebrafish swimbladder development. BMC Dev Biol. 2010;10:3 pubmed publisher
    ..As the swimbladder is lined with an intricate network of blood capillaries, it is of interest to investigate the role of the vascular system during early development of swimbladder...
  11. Korzh S, Pan X, Garcia Lecea M, Winata C, Pan X, Wohland T, et al. Requirement of vasculogenesis and blood circulation in late stages of liver growth in zebrafish. BMC Dev Biol. 2008;8:84 pubmed publisher
  12. Yang Q, Yan C, Gong Z. Activation of liver stromal cells is associated with male-biased liver tumor initiation in xmrk and Myc transgenic zebrafish. Sci Rep. 2017;7:10315 pubmed publisher
  13. Xu H, Li C, Suklai P, Zeng Q, Chong R, Gong Z. Differential sensitivities to dioxin-like compounds PCB 126 and PeCDF between Tg(cyp1a:gfp) transgenic medaka and zebrafish larvae. Chemosphere. 2018;192:24-30 pubmed publisher
    ..The findings suggested species-specific sensitivity to DLCs in fish and will facilitate choosing a sensitive and reliable fish model or tool to evaluate the risk of dioxins and DLCs exposure...
  14. Lu J, Ho Y, Ciou S, Gong Z. Innovative Disease Model: Zebrafish as an In Vivo Platform for Intestinal Disorder and Tumors. Biomedicines. 2017;5: pubmed publisher
    ..This review demonstrates that excellent zebrafish models can provide novel insights into the pathogenesis of gastrointestinal diseases and help facilitate the evaluation of novel anti-tumor drugs. ..
  15. request reprint
    Gong Z, Ju B, Wang X, He J, Wan H, Sudha P, et al. Green fluorescent protein expression in germ-line transmitted transgenic zebrafish under a stratified epithelial promoter from keratin8. Dev Dyn. 2002;223:204-15 pubmed
  16. Lui E, Li C, Li Z, Gong Z, Lam S. Molecular insights of organochlorine biocide-induced toxicity in zebrafish: Whole-adult-organism toxicogenomics, targeted gene expression and histological analyses. J Genet Genomics. 2016;43:525-8 pubmed publisher
  17. Yan C, Huo X, Wang S, Feng Y, Gong Z. Stimulation of hepatocarcinogenesis by neutrophils upon induction of oncogenic kras expression in transgenic zebrafish. J Hepatol. 2015;63:420-8 pubmed publisher
    ..An inflammatory cue from oncogenic hepatocytes upon induction of kras(V12) expression causes a rapid recruitment of neutrophils to oncogenic liver and the neutrophils play a promoting role in early hepatocarcinogenesis. ..
  18. request reprint
    Gong Z, Wan H, Tay T, Wang H, Chen M, Yan T. Development of transgenic fish for ornamental and bioreactor by strong expression of fluorescent proteins in the skeletal muscle. Biochem Biophys Res Commun. 2003;308:58-63 pubmed
    ..Furthermore, compared to the wild-type fish, these fluorescent transgenic fish have no advantage in survival and reproduction. ..