Affiliation: National University of Singapore
Shen W, Gao Z. Quantum dots and duplex-specific nuclease enabled ultrasensitive detection and serotyping of Dengue viruses in one step in a single tube. Biosens Bioelectron. 2015;65:327-32 pubmed publisher
..The highly efficient DSN cleavage in conjunction with its excellent mismatch discrimination ability permits serotyping of DVs in one tube with excellent sensitivity and selectivity. ..
Deng H, Gao Z. Bioanalytical applications of isothermal nucleic acid amplification techniques. Anal Chim Acta. 2015;853:30-45 pubmed publisher
..Representative examples illustrating the performance and advantages of each isothermal amplification technique are discussed along with some discussion on the advantages and disadvantages of each technique. ..
Deng H, Shen W, Gao Z. Colorimetric detection of single nucleotide polymorphisms in the presence of 10Â³-fold excess of a wild-type gene. Biosens Bioelectron. 2015;68:310-315 pubmed publisher
..40 fM and a dynamic range from 1.0 to 200 fM. The simple protocol and its high sensitivity and selectivity allowed the proposed assay to be used in the detection of SNPs in genomic DNA samples. ..
Shen W, Yeo K, Gao Z. A simple and highly sensitive fluorescence assay for microRNAs. Analyst. 2015;140:1932-8 pubmed publisher
..Successful attempts were made in applying the proposed assay to detect let-7a in total RNA extracted from cultured cells. ..
Yang X, Gao Z. Enzyme-catalysed deposition of ultrathin silver shells on gold nanorods: a universal and highly efficient signal amplification strategy for translating immunoassay into a litmus-type test. Chem Commun (Camb). 2015;51:6928-31 pubmed publisher
Ma Q, Gao Z. A simple and ultrasensitive fluorescence assay for single-nucleotide polymorphism. Anal Bioanal Chem. 2018;410:3093-3100 pubmed publisher
..Graphical abstract A simple and label-free fluorescence assay for SNP detection by coupling BRCA with selective fluorescence detection of pyrophosphate using the terpyridine-Zn(II) complex. ..
Deng H, Peng S, Gao Z. Highly sensitive detection of M.SssI DNA methyltransferase activity using a personal glucose meter. Anal Bioanal Chem. 2016;408:5867-72 pubmed publisher
..SssI MTase. In addition, inhibition studies with 5'-azacytidine demonstrate the capability of inhibition screening using this method. Graphical abstract Deteciton of M.SssI DNA methyltransferase activity by a personal glucose meter. ..
Png S, Seah S, Gao Z. Optical Microscopy for Detecting Binding on Small Molecule Microarrays. Methods Mol Biol. 2017;1518:109-129 pubmed
..The sample preparation steps and protocols of the two techniques to study the interactions between SMMs and their protein targets are elaborated in great detail. ..
Lim S, Shen W, Gao Z. Carbon quantum dots and their applications. Chem Soc Rev. 2015;44:362-81 pubmed publisher