Research Topics
Genomes and Genes | Guillaume BourqueSummaryCountry: Singapore Publications
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Publications
Computational tools for the analysis of rearrangements in mammalian genomesGlenn Tesler
Department of Mathematics, University of California San Diego, La Jolla, CA
Methods Mol Biol 422:145-70. 2008..We describe three computational tools (GRIMM-Synteny, GRIMM, and MGR) that can be used separately or in succession to contrast different organisms at the genome-level to exploit large-scale rearrangements as a phylogenetic character...
In silico tandem affinity purification refines an Oct4 interaction listClara Yujing Cheong
Stem Cell and Developmental Biology Group, Genome Institute of Singapore, 60 Biopolis Street, 138672, Singapore
Stem Cell Res Ther 2:26. 2011..Unearthing the interactions of Oct4 would provide insight into how this transcription factor is central to cell fate and stem cell pluripotency...
Multiplatform genome-wide identification and modeling of functional human estrogen receptor binding sitesVinsensius B Vega
Estrogen Receptor Biology Program, Genome Institute of Singapore, 60 Biopolis Street, Republic of Singapore 138672
Genome Biol 7:R82. 2006..Other factors, in addition to primary nucleotide sequence, will ultimately determine binding site selection...
Evolution of the mammalian transcription factor binding repertoire via transposable elementsGuillaume Bourque
Computational and Mathematical Biology, Genome Institute of Singapore, Singapore 138672, Singapore
Genome Res 18:1752-62. 2008..Our results demonstrate that transcriptional regulatory networks are highly dynamic in eukaryotic genomes and that transposable elements play an important role in expanding the repertoire of binding sites...
Transposable elements in gene regulation and in the evolution of vertebrate genomesGuillaume Bourque
Genome Institute of Singapore, Singapore 138672, Singapore
Curr Opin Genet Dev 19:607-12. 2009..I will also summarize results that show how transposable elements have been a major catalyst of structural rearrangements throughout evolution...
Comparative architectures of mammalian and chicken genomes reveal highly variable rates of genomic rearrangements across different lineagesGuillaume Bourque
Genome Institute of Singapore, Singapore 138672, Republic of Singapore
Genome Res 15:98-110. 2005....
Improving gene network inference by comparing expression time-series across species, developmental stages or tissuesGuillaume Bourque
Centre de Recherches Mathématiques, Universite de Montreal, Montreal, Quebec, H3C 3J7, Canada
J Bioinform Comput Biol 2:765-83. 2004..We show how this comparative framework can facilitate the recovery of the networks and improve the quality of the solutions inferred...
The convergence of cytogenetics and rearrangement-based models for ancestral genome reconstructionGuillaume Bourque
Information and Mathematical Sciences, Genome Institute of Singapore, 138672 Singapore
Genome Res 16:311-3. 2006
An oestrogen-receptor-alpha-bound human chromatin interactomeMelissa J Fullwood
Genome Institute of Singapore, Agency for Science, Technology and Research, Singapore 138672
Nature 462:58-64. 2009..We propose that chromatin interactions constitute a primary mechanism for regulating transcription in mammalian genomes...
Whole-genome mapping of histone H3 Lys4 and 27 trimethylations reveals distinct genomic compartments in human embryonic stem cellsXiao Dong Zhao
Genome Technology and Biology Group, Genome Institute of Singapore, 138672, Singapore
Cell Stem Cell 1:286-98. 2007..These global histone methylation maps provide an epigenetic framework that enables the discovery of novel transcriptional networks and delineation of different genetic compartments of the pluripotent cell genome...
Transposable elements have rewired the core regulatory network of human embryonic stem cellsGalih Kunarso
Computational and Mathematical Biology, Genome Institute of Singapore, Singapore, Singapore
Nat Genet 42:631-4. 2010..These data indicate that species-specific transposable elements have substantially altered the transcriptional circuitry of pluripotent stem cells...
Comprehensive long-span paired-end-tag mapping reveals characteristic patterns of structural variations in epithelial cancer genomesAxel M Hillmer
Genome Technology and Biology, Genome Institute of Singapore, Singapore 138672, Singapore
Genome Res 21:665-75. 2011....
webMGR: an online tool for the multiple genome rearrangement problemChi Ho Lin
Genome Institute of Singapore, 60 Biopolis Street, 02 01, Genome, Singapore 138672
Bioinformatics 26:408-10. 2010..Moreover, we have developed a web server (webMGR) that includes elaborate web output to facilitate navigation through the results...
Integration of external signaling pathways with the core transcriptional network in embryonic stem cellsXi Chen
Gene Regulation Laboratory, Genome Institute of Singapore, Singapore 138672
Cell 133:1106-17. 2008..Collectively, the comprehensive mapping of TF-binding sites identifies important features of the transcriptional regulatory networks that define ES-cell identity...
Inferring direct regulatory targets of a transcription factor in the DREAM2 challengeVinsensius B Vega
Genome Institute of Singapore, Singapore
Ann N Y Acad Sci 1158:215-23. 2009....
Long span DNA paired-end-tag (DNA-PET) sequencing strategy for the interrogation of genomic structural mutations and fusion-point-guided reconstruction of ampliconsFei Yao
Genome Institute of Singapore, Agency for Science, Technology and Research, Singapore, Singapore
PLoS ONE 7:e46152. 2012..This provides a key advantage in studying rearrangements in cancer, and we show how it can be used in a fusion-point-guided-concatenation algorithm to study focally amplified regions in cancer...
Molecular conservation of estrogen-response associated with cell cycle regulation, hormonal carcinogenesis and cancer in zebrafish and human cancer cell linesSiew Hong Lam
Genome Institute of Singapore, 02 01Genome, 60 Biopolis Street, 138672 Singapore
BMC Med Genomics 4:41. 2011..In order to determine the potential of zebrafish model for estrogen-related cancer research, we investigated the molecular conservation of estrogen responses operating in both zebrafish and human cancer cell lines...
Recovering genome rearrangements in the mammalian phylogenyHao Zhao
Computational and Mathematical Biology, Genome Institute of Singapore, Singapore
Genome Res 19:934-42. 2009..Together, this substantiates the model that regions of high sequence identity have been associated with rearrangement events throughout the mammalian phylogeny...
Regulation of estrogen receptor-mediated long range transcription via evolutionarily conserved distal response elementsYou Fu Pan
Cancer Biology and Pharmacology, Genome Institute of Singapore, Agency for Science, Technology and Research, 60 Biopolis St, 02 01 Genome, Singapore 138672
J Biol Chem 283:32977-88. 2008....
Reconstructing the genomic architecture of ancestral mammals: lessons from human, mouse, and rat genomesGuillaume Bourque
, , Canada H3C 3J7
Genome Res 14:507-16. 2004..Our analysis implies that the rate of rearrangements is much higher in murid rodents than in the human lineage and confirms the existence of rearrangement hot-spots in all three lineages...
Conserved and non-conserved enhancers direct tissue specific transcription in ancient germ layer specific developmental control genesSumantra Chatterjee
Stem Cell and Developmental Biology, Genome Institute of Singapore, 60 Biopolis Street, 138672, Singapore
BMC Dev Biol 11:63. 2011..The pressing need is to integrate the tools of comparative genomics with knowledge of developmental biology to validate enhancers for developmental transcription factors in greater detail..
Fusion transcripts and transcribed retrotransposed loci discovered through comprehensive transcriptome analysis using Paired-End diTags (PETs)Yijun Ruan
Genome Technology and Biology Group, Genome Institute of Singapore, Singapore 138672, Singapore
Genome Res 17:828-38. 2007..The PET mapping strategy presented here promises to be a useful tool in annotating the human genome, especially aberrations in human cancer genomes...
CTCF-mediated functional chromatin interactome in pluripotent cellsLusy Handoko
Genome Institute of Singapore, Singapore
Nat Genet 43:630-8. 2011..This highly complex nuclear organization offers insights toward the unifying principles that govern genome plasticity and function...
Whole-genome cartography of estrogen receptor alpha binding sitesChin Yo Lin
Genome Institute of Singapore, Singapore, Republic of Singapore
PLoS Genet 3:e87. 2007..Taken together, this genome-scale analysis suggests complex but definable rules governing ERalpha binding and gene regulation...
Transcriptional consequences of genomic structural aberrations in breast cancerKoichiro Inaki
Cancer Biology and Pharmacology, Genome Institute of Singapore, Genome, Singapore 138672, Singapore
Genome Res 21:676-87. 2011..This gene fusion is caused by tandem duplication on 17q23 and appears to be an indicator of local genomic instability altering the expression of oncogenic components such as MIR21 and RPS6KB1...
Computational tools for the analysis of rearrangements in mammalian genomesGuillaume Bourque
Genome Institute of Singapore, Singapore, Republic of Singapore
Methods Mol Biol 452:431-55. 2008....
PPARG Binding Landscapes in Macrophages Suggest a Genome-Wide Contribution of PU.1 to Divergent PPARG Binding in Human and MouseSebastian Pott
Cancer Biology and Pharmacology 2, Genome Institute of Singapore, Singapore, Singapore Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden
PLoS ONE 7:e48102. 2012....
The Oct4 and Nanog transcription network regulates pluripotency in mouse embryonic stem cellsYuin Han Loh
Gene Regulation Laboratory, Genome Institute of Singapore, Singapore 138672
Nat Genet 38:431-40. 2006..The emerging picture is one in which Oct4 and Nanog control a cascade of pathways that are intricately connected to govern pluripotency, self-renewal, genome surveillance and cell fate determination...
Success in the DREAM3 signaling response challenge using simple weighted-average imputation: lessons for community-wide experiments in systems biologyNeil D Clarke
Genome Institute of Singapore, Singapore
PLoS ONE 5:e8417. 2010....
A comparative synteny map of Burkholderia species links large-scale genome rearrangements to fine-scale nucleotide variation in prokaryotesChi Ho Lin
Genome Institute of Singapore, Singapore
Mol Biol Evol 25:549-58. 2008..Our results suggest that in prokaryotes, genome rearrangements may influence functional diversity through the enhanced divergence of boundary genes and the creation of foci for acquiring and deleting species-specific genes...
Genome sequence of the Brown Norway rat yields insights into mammalian evolutionRichard A Gibbs
Human Genome Sequencing Center, Department of Molecular and Human Genetics, Baylor College of Medicine, MS BCM226, One Baylor Plaza, Houston, Texas 77030, USA <http://www.hgsc.bcm.tmc.edu
Nature 428:493-521. 2004....
Sequence and comparative analysis of the chicken genome provide unique perspectives on vertebrate evolutionLadeana W Hillier
Genome Sequencing Center, Washington University School of Medicine, Campus Box 8501, 4444 Forest Park Avenue, St Louis, Missouri 63108, USA
Nature 432:695-716. 2004..The distinctive properties of avian microchromosomes, together with the inferred patterns of conserved synteny, provide additional insights into vertebrate chromosome architecture...
Reconstructing the genomic architecture of mammalian ancestors using multispecies comparative mapsWilliam J Murphy
Laboratory of Genomic Diversity, National Cancer Institute, Frederick, MD 21702, USA
Hum Genomics 1:30-40. 2003....
Dynamics of mammalian chromosome evolution inferred from multispecies comparative mapsWilliam J Murphy
Department of Veterinary Integrative Biosciences, Texas A and M University, College Station, TX 77843, USA
Science 309:613-7. 2005..We found that segmental duplications populate the majority of primate-specific breakpoints and often flank inverted chromosome segments, implicating their role in chromosomal rearrangement...
Genome-scale evolution: reconstructing gene orders in the ancestral speciesGuillaume Bourque
Department of Mathematics, University of Southern California, California 90089, USA
Genome Res 12:26-36. 2002..In particular, our analysis suggests a new improved rearrangement scenario for a very difficult Campanulaceae cpDNA dataset and a putative rearrangement scenario for human, mouse and cat genomes...
Genomewide expression profiling in the zebrafish embryo identifies target genes regulated by Hedgehog signaling during vertebrate developmentJun Xu
Institute of Molecular and Cell Biology, Singapore
Genetics 174:735-52. 2006..Our data also demonstrate for the first time the functionality of the Gli-binding motif in the control of Hedgehog signaling-induced gene expression in the zebrafish embryo...
Initial sequence and comparative analysis of the cat genomeJoan U Pontius
Laboratory of Genomic Diversity, SAIC Frederick, Inc, NCI Frederick, Frederick, Maryland 21702, USA
Genome Res 17:1675-89. 2007..9-fold) coverage mammal genome sequence...
