Stem cells in the treatment of amyotrophic lateral sclerosis (ALS)Vincenzo Silani
Department of Neurology, Laboratory of Neuroscience, Dino Ferrari Center, University of Milan Medical School, IRCCS Istituto Auxologico Italiano, Via Spagnoletto 3, 1 20149 Milano, Italy
Amyotroph Lateral Scler Other Motor Neuron Disord 3:173-81. 2002
..Stem cell technology is at an early stage but the desperate need for a therapy in ALS patients may legitimize clinical trials in absence of conclusive scientific evidence. This paper discusses the premises for stem cell therapy in ALS...
- Guidelines for the preclinical in vivo evaluation of pharmacological active drugs for ALS/MND: report on the 142nd ENMC international workshop
Albert C Ludolph
Department of Neurology, University of Ulm, Ulm, Germany
Amyotroph Lateral Scler 8:217-23. 2007
..In this workshop, clinical and preclinical researchers established in the field of ALS/MND met in Holland in March 2006 in order to establish guidelines for the community for drug testing in mouse models...
The diagnosis of Amyotrophic lateral sclerosis in 2010Vincenzo Silani
Department of Neurology and Laboratory of Neuroscience, Dino Ferrari Center, Universita degli Studi di Milano, Milan, Italy
Arch Ital Biol 149:5-27. 2011
..Prompt diagnosis, sensitive communication of the diagnosis, the involvement of the patient and family, a positive care plan are pre-requisites for the good clinical management of ALS patients...
- Stem cell transplantation in multiple sclerosis: safety and ethics
Vincenzo Silani
Department of Neurology and Laboratory of Neuroscience, Dino Ferrari Center, University of Milan Medical School, IRCCS Istituto Auxologico Italiano, Milano, Italy
J Neurol Sci 265:116-21. 2008
..In conclusion, although HCST is today an established therapeutical option for MS patients, safety and ethical issues need to be further clarified...
- Cell-replacement therapy with stem cells in neurodegenerative diseases
Vincenzo Silani
Department of Neurology and Laboratory of Neuroscience Dino Ferrari Center, University of Milan Medical School IRCCS Istituto Auxologico Italiano
Curr Neurovasc Res 1:283-9. 2004
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- Stem cells in amyotrophic lateral sclerosis: motor neuron protection or replacement?
V Silani
Department of Neurology and Laboratory of Neuroscience, Dino Ferrari Center, Università degli Studi di Milano IRCCS Istituto Auxologico Italiano, Milano, Italy
CNS Neurol Disord Drug Targets 9:314-24. 2010
..The review discusses relevant topics regarding the application of stem cell research to ALS focusing on their therapeutic relevance and mechanisms of action...
- Stem therapy for ALS: hope and reality
Vincenzo Silani
Amyotroph Lateral Scler Other Motor Neuron Disord 4:8-10. 2003
..Foetal cells are, in addition, post-mitotic and cannot be expanded or stored for long periods, necessitating close synchronisation of tissue donation and neurosurgery...
- Ubiquilin 2 mutations in Italian patients with amyotrophic lateral sclerosis and frontotemporal dementia
Cinzia Gellera
Unit of Genetics of Neurodegenerative and Metabolic Diseases, Fondazione IRCCS Istituto Neurologico Carlo Besta, Milan, Italy
J Neurol Neurosurg Psychiatry 84:183-7. 2013
..The aim of our study was to analyse the UBQLN2 gene in a large cohort of patients with familial (FALS) and sporadic (SALS) amyotrophic lateral sclerosis, with or without frontotemporal dementia (FTD), and in patients with FTD...
- C9ORF72 repeat expansion in a large Italian ALS cohort: evidence of a founder effect
Antonia Ratti
Department of Neurology and Laboratory of Neuroscience, IRCCS Istituto Auxologico Italiano, Milan, Italy
Neurobiol Aging 33:2528.e7-14. 2012
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- ATAXIN2 CAG-repeat length in Italian patients with amyotrophic lateral sclerosis: risk factor or variant phenotype? Implication for genetic testing and counseling
Cinzia Gellera
Genetics of Neurodegenerative and Metabolic Disorders, Fondazione IRCCS, Istituto Neurologico Carlo Besta, Milano, Italy
Neurobiol Aging 33:1847.e15-21. 2012
..Overlapping phenotypes should be considered in genetic testing and counseling, both for patients and at-risk family members...
- Neuroprotective effects of human mesenchymal stem cells on neural cultures exposed to 6-hydroxydopamine: implications for reparative therapy in Parkinson's disease
Lidia Cova
Department of Neurology and Laboratory of Neuroscience, IRCCS Istituto Auxologico Italiano 20149, Milan, Italy
Apoptosis 17:289-304. 2012
..Our findings provide novel insights for the development of therapeutic strategies designed to counteract the neurodegenerative processes of PD...
- Multiple neurogenic and neurorescue effects of human mesenchymal stem cell after transplantation in an experimental model of Parkinson's disease
Lidia Cova
Department of Neurology and Laboratory of Neuroscience, Centro Dino Ferrari, Università degli Studi di Milano IRCCS Istituto Auxologico Italiano, 20149 Milan, Italy
Brain Res 1311:12-27. 2010
..Altogether, our data provide remarkable cues regarding the potential of hMSCs in promoting endogenous reparative mechanisms that may prove applicable and beneficial for PD treatment...
TDP-43 and FUS RNA-binding proteins bind distinct sets of cytoplasmic messenger RNAs and differently regulate their post-transcriptional fate in motoneuron-like cellsClaudia Colombrita
Department of Neurology and Laboratory of Neuroscience, IRCCS Istituto Auxologico Italiano, Milan 20149, Italy
J Biol Chem 287:15635-47. 2012
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- Validation of a Neuro Virtual Reality-based version of the Multiple Errands Test for the assessment of executive functions
Simona Raspelli
Applied Technology for Neuro Psychology Lab, Istituto Auxologico Italiano, Milan, Italy
Stud Health Technol Inform 167:92-7. 2011
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- Metalloproteinase alterations in the bone marrow of ALS patients
Patrizia Bossolasco
Fondazione Matarelli, Dipartimento di Farmacologia, Chemioterapia e Tossicologia Medica, Universita degli Studi di Milano, Via Vanvitelli, 32 20129, Milan, Italy
J Mol Med (Berl) 88:553-64. 2010
..Therefore, therapeutic implantation of autologous BM derived SC in ALS patients needs to be carefully reevaluated...
- TDP-43 is recruited to stress granules in conditions of oxidative insult
Claudia Colombrita
Department of Neurology and Laboratory of Neuroscience, Dino Ferrari Center, Università degli Studi di Milano IRCCS Istituto Auxologico Italiano, Milan, Italy
J Neurochem 111:1051-61. 2009
..Although SGs were not evident in ALS brains, we speculate that an altered control of mRNA translation in stressful conditions may trigger motor neuron degeneration at early stages of the disease...
- Stem cell therapy for neurodegenerative diseases: the issue of transdifferentiation
Lidia Cova
Department of Neurology and Laboratory of Neuroscience, Dino Ferrari Center, University of Milan Medical School, IRCCS Istituto Auxologico Italiano, Milan, Italy
Stem Cells Dev 13:121-31. 2004
..This review summarizes recent advances gained in stem cell research applied to neurodegenerative diseases with a special emphasis to the criticisms put forward...
- Screening of the PFN1 gene in sporadic amyotrophic lateral sclerosis and in frontotemporal dementia
Cinzia Tiloca
Department of Neurology and Laboratory of Neuroscience, IRCCS Istituto Auxologico Italiano, Milan, Italy Scuola di Dottorato di Medicina Molecolare, Universita degli Studi di Milano, Milan, Italy Electronic address
Neurobiol Aging 34:1517.e9-1517.e10. 2013
..G15G in another patient, but none in a panel of 1512 control subjects. Our results suggest that PFN1 mutations in sporadic ALS and in FTD are rare, at least in the Italian population...
- Oligoclonal bands in the cerebrospinal fluid of amyotrophic lateral sclerosis patients with disease-associated mutations
Nicola Ticozzi
Department of Neurology and Laboratory of Neuroscience, IRCCS Istituto Auxologico Italiano, Piazzale Brescia 20, 20149, Milan, Italy
J Neurol 260:85-92. 2013
..The role of mutant TARDBP and ANG genes on BBB integrity of ALS patients warrants further investigation...
- Impaired expression of insulin-like growth factor-1 system in skeletal muscle of amyotrophic lateral sclerosis patients
Christian Lunetta
Department of Neurology and Laboratory of Neuroscience, Dino Ferrari Center, IRCCS Istituto Auxologico Italiano, Universita degli Studi di Milano, Milano, Italy
Muscle Nerve 45:200-8. 2012
..Adult muscle fibers are a source of growth factors, including insulin-like growth factor-1 (IGF-1). These factors influence neuronal survival, axonal growth, and maintenance of synaptic connections...
- Huntington's disease: the current state of research with peripheral tissues
Jenny Sassone
Department of Neurology and Laboratory of Neuroscience, Dino Ferrari Center, IRCCS Istituto Auxologico Italiano, University of Milan Medical School, Via Spagnoletto 3, 20149, Milan, Italy
Exp Neurol 219:385-97. 2009
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- Molecular and phenotypical characterization of human amniotic fluid cells and their differentiation potential
Tiziana Montemurro
Cell Factory, Centro di Medicina Trasfusionale, Terapia Cellulare e Criobiologia, Fondazione IRCCS Ospedale Maggiore Policlinico, Mangiagalli e Regina Elena, Milan, Italy
Biomed Mater Eng 18:183-5. 2008
- Stem-cell therapy for amyotrophic lateral sclerosis
Vincenzo Silani
Department of Neurology and Laboratory of Neuroscience, Dino Ferrari Centre, University of Milan Medical School IRCCS Istituto Auxologico Italiano, Milano, Italy
Lancet 364:200-2. 2004
..Stem-cell therapy will need to be used with other drugs or treatments, such as antioxidants and/or infusion of trophic molecules...
- Production of monocyte chemoattractant protein-1 in amyotrophic lateral sclerosis
PierLuigi Baron
Department of Neurological Sciences, Dino Ferrari Center, University of Milan Medical School, IRCCS Ospedale Maggiore Policlinico, Via S Sforza 35, 20122 Milan, Italy
Muscle Nerve 32:541-4. 2005
..Our findings suggest a role for MCP-1 as an important molecular mediator of the injury response in ALS...
- Low anaerobic threshold and increased skeletal muscle lactate production in subjects with Huntington's disease
Andrea Ciammola
Department of Neurology, Centro Dino Ferrari Università degli Studi di Milano IRCCS Istituto Auxologico Italiano, Milan, Italy
Mov Disord 26:130-7. 2011
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- Amyotrophic lateral sclerosis and frontotemporal dementia (ALS-FTD)
Stefano Zago
Department of Neurological Sciences, Universita degli Studi di Milano, Medical School IRCCS, Fondazione Ca Granda Ospedale Maggiore Policlinico, IRCCS Istituto Auxologico Italiano, Milano, Italy
Arch Ital Biol 149:39-56. 2011
..It is our intent to provide a brief overview of the relationships between ALS and FTD...
- Identification of new ANG gene mutations in a large cohort of Italian patients with amyotrophic lateral sclerosis
Cinzia Gellera
Division of Biochemistry and Genetics, Fondazione IRCCS Instituto Neurologico Carlo Besta, Milan, Italy
Neurogenetics 9:33-40. 2008
..Our results provide further evidence of a tight link between angiogenesis and ALS pathogenesis and suggest that mutations in ANG gene are associated with an increased risk to develop ALS...
- Mutational analysis of VCP gene in familial amyotrophic lateral sclerosis
Cinzia Tiloca
Department of Neurology and Laboratory of Neuroscience, IRCCS, Istituto Auxologico Italiano, Milan, Italy
Neurobiol Aging 33:630.e1-2. 2012
..Bioinformatical analyses predicted no changes in splicing process or microRNA binding sites. Our results do not confirm a main contribution of VCP gene to familial ALS in the Italian population...
- Aripiprazole in the treatment of Huntington's disease: a case series
Andrea Ciammola
Department of Neurology and Laboratory of Neuroscience, Dino Ferrari Centre, University of Milan Medical School IRCCS Istituto Auxologico Italiano, Milano, Italy
Neuropsychiatr Dis Treat 5:1-4. 2009
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- Intracerebral haemorrhage, a possible presentation in Churg-Strauss syndrome: case report and review of the literature
Niccolò E Mencacci
Department of Neurology and Laboratory of Neuroscience, Dino Ferrari Centre, Università degli Studi di Milano IRCCS Istituto Auxologico Italiano, Milan, Italy
J Neurol Sci 301:107-11. 2011
..Our case points out that intracerebral haemorrhage can be, despite rare, a presenting feature of CSS. Previously reported patients affected by cerebral haemorrhage and CSS are summarized and briefly reviewed...
- Longitudinal tracking of human fetal cells labeled with super paramagnetic iron oxide nanoparticles in the brain of mice with motor neuron disease
Paolo Bigini
Mario Negri Institute for Pharmacological Research, Milan, Italy
PLoS ONE 7:e32326. 2012
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- Post-transcriptional regulation of neuro-oncological ventral antigen 1 by the neuronal RNA-binding proteins ELAV
Antonia Ratti
Department of Neurological Sciences, Dino Ferrari Centre, University of Milan Medical School, IRCCS Istituto Auxologico Italiano, 20095 Cusano, Milan, Italy
J Biol Chem 283:7531-41. 2008
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- Induction of neurotrophin expression via human adult mesenchymal stem cells: implication for cell therapy in neurodegenerative diseases
Federica Pisati
Fondazione IRCCS Ospedale Maggiore, Department of Neurological Sciences, Stem Cell Laboratory, Dino Ferrari Center, University of Milan, Milan, Italy
Cell Transplant 16:41-55. 2007
..hMSCs are further defined in their transplantation potential for treating neurological disorders...
- Charles Bonnet syndrome: two case reports and review of the literature
Alberto Lerario
Department of Neurology and Laboratory of Neuroscience, IRCCS Istituto Auxologico Italiano, 20149, Milan, Italy
J Neurol 260:1180-6. 2013
..Nonpharmacological interventions aimed to reduce the visual pathway deprivation. Reassurance of the benign nature of CBS is essential to support patients and reduce caregiver's burden...
- Wake-up stroke and TIA due to paradoxical embolism during long obstructive sleep apnoeas: a cross-sectional study
Alfonso Ciccone
Stroke Unit and Department of Neurology, Niguarda Ca Granda Hospital, Milan, Italy
Thorax 68:97-104. 2013
..We investigated whether the combination of LOSA and RLSh is associated with ischaemic stroke or transient ischaemic attack (TIA) on waking (wake-up stroke)...
- Cobalamin as a regulator of serum and cerebrospinal fluid levels of normal prions
Giuseppe Scalabrino
Città Studi Department, Laboratory of Neuropathology, University of Milan, Milan, Italy
J Clin Neurosci 20:134-8. 2013
..We found a correlation between Cbl and PrP(C) levels in the serum and CSF of Cbl-D patients, which suggests that Cbl may regulate the PrP(C) levels in the serum and CSF in humans...
- Implementation of the multiple errands test in a NeuroVR-supermarket: a possible approach
Simona Raspelli
Applied Technology for Neuro Psychology Lab, Istituto Auxologico Italiano, Milano, Milan, Italy
Stud Health Technol Inform 154:115-9. 2010
..The specific goal of this study was to conduct a pilot study using the virtual version of MET (VMET) with both control subjects and patients with cognitive impairment derived from stroke...
- Mitochondrial respiratory chain dysfunction in muscle from patients with amyotrophic lateral sclerosis
Veronica Crugnola
Department of Neurological Sciences, Dino Ferrari Center, Universita degli Studi di Milano, Scientific Institute for Research and Treatment Foundation Ospedale Maggiore Policlinico, Mangiagalli and Regina Elena, Milan, Italy
Arch Neurol 67:849-54. 2010
..Although defects of the mitochondrial respiratory chain have been described in several ALS patients, their pathogenic significance is unclear...
- Good practice in the management of amyotrophic lateral sclerosis: clinical guidelines. An evidence-based review with good practice points. EALSC Working Group
Peter Munch Andersen
Department of Neurology, Umea University Hospital, Umea, Sweden
Amyotroph Lateral Scler 8:195-213. 2007
..During the course of the disease, every effort should be made to maintain patient autonomy. Advance directives for palliative end of life care are important and should be discussed early with the patient and relatives if they so wish...
- A role for the ELAV RNA-binding proteins in neural stem cells: stabilization of Msi1 mRNA
Antonia Ratti
Department of Neuroscience, Dino Ferrari Centre, University of Milan IRCCS Istituto Auxologico Italiano, Via Zucchi 18, 20095 Cusano Milanino, Italy
J Cell Sci 119:1442-52. 2006
..We propose that ELAV RNA-binding proteins exert an important post-transcriptional control on Musashi-1 expression in the transition from proliferation to neural differentiation of stem/progenitor cells...
- Report from the Italian Ministerial Committee regarding the diagnosis, care and assistance of patients with ALS
Caterina Bendotti
Amyotroph Lateral Scler Other Motor Neuron Disord 6:5-7. 2005
