Roland K O Sigel



  1. Pechlaner M, Dominguez Martin A, Sigel R. Influence of pH and Mg(ii) on the catalytic core domain 5 of a bacterial group II intron. Dalton Trans. 2017;46:3989-3995 pubmed publisher
    ..The influence of Mg(ii) and pH on the local conformation of the catalytically crucial region is also explored by fluorescence spectroscopy. ..
  2. Paudel B, Fiorini E, Börner R, Sigel R, Rueda D. Optimal molecular crowding accelerates group II intron folding and maximizes catalysis. Proc Natl Acad Sci U S A. 2018;115:11917-11922 pubmed publisher
    ..Therefore, this study shows that optimal molecular crowding concentrations help the ribozyme not only to reach the native fold but also to increase its in vitro activity to approach that in physiological conditions. ..
  3. Freisinger E, Sigel R. The Bioinorganic Periodic Table. Chimia (Aarau). 2019;73:185-193 pubmed publisher
  4. Steffen F, Börner R, Freisinger E, Sigel R. Stick, Flick, Click: DNA-guided Fluorescent Labeling of Long RNA for Single-molecule FRET. Chimia (Aarau). 2019;73:257-261 pubmed publisher
    ..A special focus lies on our recently developed, DNA-guided approach that inserts two fluorophores through a stepwise process of templated functionality transfer and click chemistry. ..
  5. Schmitz A, Zelger Paulus S, Gasser G, Sigel R. Strategy for Internal Labeling of Large RNAs with Minimal Perturbation by Using Fluorescent PNA. Chembiochem. 2015;16:1302-6 pubmed publisher
    ..The folding pathway and catalytic activity of this large ribozyme is only minimally affected by the PNA, but the background signal is significantly reduced. ..
  6. Böttcher A, Kowerko D, Sigel R. Explicit analytic equations for multimolecular thermal melting curves. Biophys Chem. 2015;202:32-9 pubmed publisher
    ..As supporting information, open-source Matlab scripts for the computer implementation of the equations are provided. ..
  7. Schaffer M, Choudhary P, Sigel R. The AdoCbl-riboswitch interaction investigated by in-line probing and surface plasmon resonance spectroscopy (SPR). Methods Enzymol. 2014;549:467-88 pubmed publisher
    ..Owing to the light sensitivity of the cobalt(I)'carbon bond, these methods were specifically modified to ensure the chemical integrity of AdoCbl. ..
  8. Kowerko D, König S, Skilandat M, Kruschel D, Hadzic M, Cardo L, et al. Cation-induced kinetic heterogeneity of the intron-exon recognition in single group II introns. Proc Natl Acad Sci U S A. 2015;112:3403-8 pubmed publisher
    ..Unconventional kinetics in nucleic acid folding frequently encountered in single-molecule experiments are therefore likely to originate from a spectrum of conformations that differ in the occupation of M(2+) binding sites. ..
  9. Pechlaner M, Donghi D, Zelenay V, Sigel R. Protonation-Dependent Base Flipping at Neutral pH in the Catalytic Triad of a Self-Splicing Bacterial Group II Intron. Angew Chem Int Ed Engl. 2015;54:9687-90 pubmed publisher
    ..Hence, this here identified shifted pK(a) value controls the conformational change between the two steps of splicing. ..

More Information


  1. Bártová S, Pechlaner M, Donghi D, Sigel R. Studying metal ion binding properties of a three-way junction RNA by heteronuclear NMR. J Biol Inorg Chem. 2016;21:319-28 pubmed publisher
    ..These results together with our recently published data on Mg(II) interaction provide a much better understanding of Mg(II) binding to D1??, and reveal how intricate and complex metal ion interactions can be. ..
  2. Skilandat M, Rowinska Zyrek M, Sigel R. Secondary structure confirmation and localization of Mg2+ ions in the mammalian CPEB3 ribozyme. RNA. 2016;22:750-63 pubmed publisher
  3. Schaffer M, Peng G, Spingler B, Schnabl J, Wang M, Olieric V, et al. The X-ray Structures of Six Octameric RNA Duplexes in the Presence of Different Di- and Trivalent Cations. Int J Mol Sci. 2016;17: pubmed publisher
    ..Furthermore, we could demonstrate that an accurate localization of the metal ions in the X-ray structures require the consideration of several crystallographic and geometrical parameters as well as the anomalous difference map. ..
  4. request reprint
    Guiset Miserachs H, Donghi D, Börner R, Johannsen S, Sigel R. Distinct differences in metal ion specificity of RNA and DNA G-quadruplexes. J Biol Inorg Chem. 2016;21:975-986 pubmed
    ..Finally, the difference in response between NRAS and TERRA, as well as to the corresponding DNA sequences with respect to different metal ions, could potentially be exploited for selective targeting purposes. ..
  5. Erat M, Besic E, Oberhuber M, Johannsen S, Sigel R. Specific phosphorothioate substitution within domain 6 of a group II intron ribozyme leads to changes in local structure and metal ion binding. J Biol Inorg Chem. 2018;23:167-177 pubmed publisher
    ..Together with NOE data of a hexaamminecobalt(III) titration, this suggests a single metal ion binding site at the GU wobble pair above the branch point in the major groove of D6 of this group II intron ribozyme. ..