Rafat A Siddiqui

Summary

Publications

  1. pmc Attenuation of niacin-induced prostaglandin D(2) generation by omega-3 fatty acids in THP-1 macrophages and Langerhans dendritic cells
    Justin Vanhorn
    Cellular Biochemistry Laboratory, Methodist Research Institute, Indianapolis
    J Inflamm Res 5:37-50. 2012
  2. doi request reprint Characterization of lovastatin-docosahexaenoate anticancer properties against breast cancer cells
    Rafat A Siddiqui
    Cellular Biochemistry Laboratory, Methodist Research Institute, Indiana University Health, 1800 N Capitol Ave, Room E504, Indianapolis, IN 46202, USA Department of Medicine, Indiana University School of Medicine, Indianapolis, IN 46202, USA Electronic address
    Bioorg Med Chem 22:1899-908. 2014
  3. pmc Characterization of synergistic anti-cancer effects of docosahexaenoic acid and curcumin on DMBA-induced mammary tumorigenesis in mice
    Rafat A Siddiqui
    Cellular Biochemistry Laboratory, Indiana University Health, Indianapolis, IN 46202, USA
    BMC Cancer 13:418. 2013
  4. doi request reprint Docosahexaenoic acid: a natural powerful adjuvant that improves efficacy for anticancer treatment with no adverse effects
    Rafat A Siddiqui
    Cellular Biochemistry Laboratory, Indiana University Health, Indiana University School of Medicine, Indianapolis, IN 46202, USA
    Biofactors 37:399-412. 2011
  5. doi request reprint Long-chain saturated fatty acids induce pro-inflammatory responses and impact endothelial cell growth
    Kevin A Harvey
    Cellular Biochemistry Laboratory, Methodist Research Institute, Indianapolis, IN 46202, USA
    Clin Nutr 29:492-500. 2010
  6. doi request reprint Trans fatty acids: induction of a pro-inflammatory phenotype in endothelial cells
    Kevin A Harvey
    Cellular Biochemistry Laboratory, Methodist Research Institute, Indianapolis, IN 46202, USA
    Lipids 47:647-57. 2012
  7. doi request reprint n-3 fatty acids prevent whereas trans-fatty acids induce vascular inflammation and sudden cardiac death
    Rafat A Siddiqui
    Cellular Biochemistry and Lipid Biology Laboratories, Methodist Research Institute, Indianapolis, IN, USA
    Br J Nutr 102:1811-9. 2009
  8. pmc Oleic and docosahexaenoic acid differentially phase separate from lipid raft molecules: a comparative NMR, DSC, AFM, and detergent extraction study
    Saame Raza Shaikh
    Department of Biology, Indiana University Purdue University, Indianapolis, Indiana 46202 5132, USA
    Biophys J 87:1752-66. 2004
  9. pmc A synergistic antiproliferation effect of curcumin and docosahexaenoic acid in SK-BR-3 breast cancer cells: unique signaling not explained by the effects of either compound alone
    Jeffrey D Altenburg
    Cellular Biochemistry Laboratory, Methodist Research Institute, Indiana University Health, Indianapolis, Indiana, USA
    BMC Cancer 11:149. 2011
  10. pmc Anticancer properties of propofol-docosahexaenoate and propofol-eicosapentaenoate on breast cancer cells
    Rafat A Siddiqui
    Methodist Research Institute, Clarian Health Partners, Indianapolis, IN, USA
    Breast Cancer Res 7:R645-54. 2005

Collaborators

Detail Information

Publications32

  1. pmc Attenuation of niacin-induced prostaglandin D(2) generation by omega-3 fatty acids in THP-1 macrophages and Langerhans dendritic cells
    Justin Vanhorn
    Cellular Biochemistry Laboratory, Methodist Research Institute, Indianapolis
    J Inflamm Res 5:37-50. 2012
    ..In conclusion, this study suggests that the regular use of omega-3 PUFAs along with niacin can potentially reduce the niacin-induced flushing response in sensitive patients...
  2. doi request reprint Characterization of lovastatin-docosahexaenoate anticancer properties against breast cancer cells
    Rafat A Siddiqui
    Cellular Biochemistry Laboratory, Methodist Research Institute, Indiana University Health, 1800 N Capitol Ave, Room E504, Indianapolis, IN 46202, USA Department of Medicine, Indiana University School of Medicine, Indianapolis, IN 46202, USA Electronic address
    Bioorg Med Chem 22:1899-908. 2014
    ..These data suggest that the LOV-DHA conjugate mediated its effects through a HMG-CoA reductase-independent pathway and exerted significantly (P<0.05) higher anticancer effects in breast cancer cells than lovastatin or DHA alone...
  3. pmc Characterization of synergistic anti-cancer effects of docosahexaenoic acid and curcumin on DMBA-induced mammary tumorigenesis in mice
    Rafat A Siddiqui
    Cellular Biochemistry Laboratory, Indiana University Health, Indianapolis, IN 46202, USA
    BMC Cancer 13:418. 2013
    ....
  4. doi request reprint Docosahexaenoic acid: a natural powerful adjuvant that improves efficacy for anticancer treatment with no adverse effects
    Rafat A Siddiqui
    Cellular Biochemistry Laboratory, Indiana University Health, Indiana University School of Medicine, Indianapolis, IN 46202, USA
    Biofactors 37:399-412. 2011
    ..Use of DHA with anticancer treatments provides an avenue to therapeutic improvement that involves less risk or side effects for patients and reduced regulatory burden for implementation...
  5. doi request reprint Long-chain saturated fatty acids induce pro-inflammatory responses and impact endothelial cell growth
    Kevin A Harvey
    Cellular Biochemistry Laboratory, Methodist Research Institute, Indianapolis, IN 46202, USA
    Clin Nutr 29:492-500. 2010
    ..Endothelial cells (EC) play an important role in the acute inflammatory responses. We, therefore, evaluated the acute effects of different chain-length SFAs upon EC functions...
  6. doi request reprint Trans fatty acids: induction of a pro-inflammatory phenotype in endothelial cells
    Kevin A Harvey
    Cellular Biochemistry Laboratory, Methodist Research Institute, Indianapolis, IN 46202, USA
    Lipids 47:647-57. 2012
    ..In conclusion, t18:1n-9 and t18:2n-6 fatty acids effectively incorporated into the phospholipid component of endothelial cells and subsequently induce a pro-inflammatory phenotype...
  7. doi request reprint n-3 fatty acids prevent whereas trans-fatty acids induce vascular inflammation and sudden cardiac death
    Rafat A Siddiqui
    Cellular Biochemistry and Lipid Biology Laboratories, Methodist Research Institute, Indianapolis, IN, USA
    Br J Nutr 102:1811-9. 2009
    ..The in vitro study demonstrates that TFA induce, while n-3 PUFA prevent, vascular inflammation...
  8. pmc Oleic and docosahexaenoic acid differentially phase separate from lipid raft molecules: a comparative NMR, DSC, AFM, and detergent extraction study
    Saame Raza Shaikh
    Department of Biology, Indiana University Purdue University, Indianapolis, Indiana 46202 5132, USA
    Biophys J 87:1752-66. 2004
    ..We conclude that the OA-PE phase separates from rafts far less than DHA-PE, which may have implications for cellular signaling...
  9. pmc A synergistic antiproliferation effect of curcumin and docosahexaenoic acid in SK-BR-3 breast cancer cells: unique signaling not explained by the effects of either compound alone
    Jeffrey D Altenburg
    Cellular Biochemistry Laboratory, Methodist Research Institute, Indiana University Health, Indianapolis, Indiana, USA
    BMC Cancer 11:149. 2011
    ....
  10. pmc Anticancer properties of propofol-docosahexaenoate and propofol-eicosapentaenoate on breast cancer cells
    Rafat A Siddiqui
    Methodist Research Institute, Clarian Health Partners, Indianapolis, IN, USA
    Breast Cancer Res 7:R645-54. 2005
    ..The present study describes the synthesis, purification, characterization and evaluation of two novel anticancer conjugates, propofol-docosahexaenoate (propofol-DHA) and propofol-eicosapentaenoate (propofol-EPA)...
  11. doi request reprint High ACAT1 expression in estrogen receptor negative basal-like breast cancer cells is associated with LDL-induced proliferation
    Caryl J Antalis
    Cellular Biochemistry Laboratory, Methodist Research Institute, 1800 N Capitol Ave, E504, Indianapolis, IN 46202, USA
    Breast Cancer Res Treat 122:661-70. 2010
    ..Differences in lipid uptake and storage capability may at least partially explain the differential effect of a low-fat diet on human breast cancer recurrence...
  12. pmc Oleic acid inhibits stearic acid-induced inhibition of cell growth and pro-inflammatory responses in human aortic endothelial cells
    Kevin A Harvey
    Cellular Biochemistry Laboratory, Methodist Research Institute, Indianapolis, IN, USA
    J Lipid Res 51:3470-80. 2010
    ..Our data suggest that oleic acid has the ability to reduce the inflammatory effects of long-chain SFAs in HAECs through reducing cellular stearic acid incorporation and NF-κB activation...
  13. doi request reprint Role of Rho kinase in sphingosine 1-phosphate-mediated endothelial and smooth muscle cell migration and differentiation
    Kevin A Harvey
    Cellular Biochemistry Laboratory, Methodist Research Institute, Clarian Health Partners, Room E504, Indianapolis, IN, USA
    Mol Cell Biochem 342:7-19. 2010
    ..In conclusion, S1P-induced PAEC angiogenic responses are regulated by S1P(1) and/or S1P(3) receptors independent of Rho kinase activation, whereas S1P(2) receptor-mediated curtailment of PASMC function by S1P...
  14. doi request reprint Anticancer properties of oxidation products of docosahexaenoic acid
    Rafat A Siddiqui
    Methodist Research Institute, Clarian Health Partners, Indianapolis, IN 46202, United States
    Chem Phys Lipids 153:47-56. 2008
    ..We conclude that the study of the effects of lipid peroxidation products on biochemical function will be a difficult but highly rewarding area for future studies...
  15. doi request reprint Characterization of anticancer properties of 2,6-diisopropylphenol-docosahexaenoate and analogues in breast cancer cells
    Kevin A Harvey
    Cellular Biochemistry Laboratory, Methodist Research Institute, Clarian Health Partners, Inc, 1800 N Capital Ave, Indianapolis, IN 46202, USA
    Bioorg Med Chem 18:1866-74. 2010
    ..In conclusion, these results suggest that the novel PP-DHA and IPP-DHA conjugates and their amide derivatives may be useful for the treatment of breast cancer...
  16. doi request reprint A novel 2,6-diisopropylphenyl-docosahexaenoamide conjugate induces apoptosis in T cell acute lymphoblastic leukemia cell lines
    Jeffrey D Altenburg
    Cellular Biochemistry Laboratory, Methodist Research Institute, Indiana University Health, Indianapolis, IN 46202, USA
    Biochem Biophys Res Commun 411:427-32. 2011
    ..These conjugates have potential use for treatment of patients with T cell acute lymphoblastic leukemia...
  17. doi request reprint Omega-3 polyunsaturated fatty acids down-modulate CXCR4 expression and function in MDA-MB-231 breast cancer cells
    Jeffrey D Altenburg
    Cellular Biochemistry Laboratory, Methodist Research Institute, Clarian Health Partners, Indianapolis, IN 46202, USA
    Mol Cancer Res 7:1013-20. 2009
    ..This suggests a previously unreported potential benefit of n-3 PUFAs to patients with metastatic breast cancer. The data presented in this study may also translate to other disorders that involve up-regulated chemokine receptors...
  18. ncbi request reprint Modulation of lipid rafts by Omega-3 fatty acids in inflammation and cancer: implications for use of lipids during nutrition support
    Rafat A Siddiqui
    Methodist Research Institute, Cellular Biochemistry, Indianapolis, IN 46202, USA
    Nutr Clin Pract 22:74-88. 2007
    ..We review how alteration of raft lipids affects the raft/nonraft localization and hence the function of several proteins involved in cell signaling. We focus our discussion of raft-signaling proteins on inflammation and cancer...
  19. doi request reprint Docosahexaenoic acid is a substrate for ACAT1 and inhibits cholesteryl ester formation from oleic acid in MCF-10A cells
    Caryl J Antalis
    Cellular Biochemistry Laboratory, Methodist Research Institute, 1800 N Capitol Avenue, Suite E504, Indianapolis, IN 46202, USA
    Prostaglandins Leukot Essent Fatty Acids 80:165-71. 2009
    ..In the manner of a poor substrate, DHA also inhibited the activity of ACAT1, a universally expressed enzyme involved in intracellular cholesterol homeostasis, in a cell type that does not secrete lipids or express ACAT2...
  20. doi request reprint Migration of MDA-MB-231 breast cancer cells depends on the availability of exogenous lipids and cholesterol esterification
    Caryl J Antalis
    Methodist Research Institute, IU Health, Indianapolis, IN, USA
    Clin Exp Metastasis 28:733-41. 2011
    ..This data substantiates the association of lipid accumulation with aggressive behavior in an ER-breast cancer cell line...
  21. pmc Steroidal compounds in commercial parenteral lipid emulsions
    Zhidong Xu
    Methodist Research Institute, Indiana University Health, Indianapolis, IN 46202, USA
    Nutrients 4:904-21. 2012
    ..This information may help guide future studies and clinical care of patients with lipid emulsion-associated liver dysfunction...
  22. ncbi request reprint Trans-fatty acids induce pro-inflammatory responses and endothelial cell dysfunction
    Kevin A Harvey
    Cellular Biochemistry Laboratory, Methodist Research Institute, Clarian Health, 1701 N Senate Room E504, Indianapolis, IN 46202, USA
    Br J Nutr 99:723-31. 2008
    ..In conclusion, these in vitro studies demonstrated that TFA play a role in the induction of pro-inflammatory responses and endothelial cell dysfunction...
  23. doi request reprint Attenuation of proteolysis and muscle wasting by curcumin c3 complex in MAC16 colon tumour-bearing mice
    Rafat A Siddiqui
    Methodist Research Institute, Clarian Health Partners, Indianapolis, IN 46202, USA
    Br J Nutr 102:967-75. 2009
    ..In conclusion, oral curcumin c3 results in the prevention and reversal of weight loss. The data imply that curcumin c3 may be an effective adjuvant therapy against cachexia...
  24. ncbi request reprint Diverse signaling pathways regulate fibroblast differentiation and transformation through Rho kinase activation
    Kevin A Harvey
    Cellular Biochemistry Laboratory, Methodist Research Institute, Clarian Health Partners, Indianapolis, Indiana, USA
    J Cell Physiol 211:353-63. 2007
    ....
  25. ncbi request reprint Modulation of enzymatic activities by n-3 polyunsaturated fatty acids to support cardiovascular health
    Rafat A Siddiqui
    Cellular Biochemistry Laboratory, Methodist Research Institute, Clarian Health, Indianapolis, IN 46202, USA
    J Nutr Biochem 19:417-37. 2008
    ..This review is therefore focused on the molecular targets and signaling pathways that are regulated by n-3 PUFAs in relation to their cardioprotective effects...
  26. ncbi request reprint Omega-3 polyunsaturated fatty acids attenuate breast cancer growth through activation of a neutral sphingomyelinase-mediated pathway
    Min Wu
    Cellular Biochemistry Laboratory, Methodist Research Institute, Clarian Health Partners, Indianapolis, IN 46202, USA
    Int J Cancer 117:340-8. 2005
    ..05). The results suggest that inhibition of breast cancer growth in nude mice by dietary fish oil and inhibition of breast cancer cell growth in culture by treatment with DHA and EPA is mediated by activation of N-SMYase...
  27. doi request reprint Docosahexaenoic acid downregulates interferon gamma-induced expression of CXCL16 in human aortic smooth muscle cells
    Jeffrey D Altenburg
    Cellular Biochemistry Laboratory, Methodist Research Institute, Clarian Health Partners, Indianapolis, IN 46202, United States
    Biochem Biophys Res Commun 391:609-14. 2010
    ..These findings may prove to be important in other disease fields that identify IFNgamma as a regulator...
  28. ncbi request reprint Omega 3-fatty acids: health benefits and cellular mechanisms of action
    Rafat A Siddiqui
    Methodist Research Institute, Indianapolis, Indiana 46202, USA
    Mini Rev Med Chem 4:859-71. 2004
    ..Protein phosphatases are also involved with the protein Bcl2, which regulates the release of cytochrome c from mitochondria, and eventually, activation of the apoptotic enzyme caspase 3...
  29. ncbi request reprint Biologically active dietary peptides
    Gary P Zaloga
    Methodist Research Institute of Clarian Health Inc and Department of Medicine of Indiana University School of Medicine, Indianapolis, IN 46202, USA
    Mini Rev Med Chem 4:815-21. 2004
    ..We also review biologic effects of different protein sources, which generate different peptide profiles during digestion. The implications of dietary peptides for modulation of disease are discussed...
  30. ncbi request reprint Inhibition of phenylephrine-induced cardiac hypertrophy by docosahexaenoic acid
    Rafat A Siddiqui
    Cellular Biochemistry Laboratory, Methodist Research Institute, Clarian Health Partners, 1701 N Senate Blvd, Indianapolis, Indiana 46202, USA
    J Cell Biochem 92:1141-59. 2004
    ..In vivo studies are needed to confirm these in vitro effects of DHA...
  31. pmc Cell-cycle arrest in Jurkat leukaemic cells: a possible role for docosahexaenoic acid
    Rafat A Siddiqui
    Cellular Biochemistry Laboratory, Methodist Research Institute, Clarian Health Partners, 1701 N Senate Boulevard, Indianapolis, IN 46202, USA
    Biochem J 371:621-9. 2003
    ..The net effect of cdk2 inhibition and protein phosphatase activation is an inhibition of pRb phosphorylation, consequently arresting Jurkat cell growth...
  32. ncbi request reprint Acyl chain unsaturation in PEs modulates phase separation from lipid raft molecules
    Saame Raza Shaikh
    Department of Biology, Indiana University Purdue University Indianapolis, 723 W Michigan Street, Indianapolis, IN 46202 5132, USA
    Biochem Biophys Res Commun 311:793-6. 2003
    ..Our findings demonstrate that increasing unsaturation in PEs results in increased phase separation from SM/CHOL membranes, which may have implications for cellular signaling...