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..In future analyses, genome editing of synthetic target arrays for lineage tracing (GESTALT) can be used to generate large-scale maps of cell lineage in multicellular systems for normal development and disease. ..
Shendure J, Fields S. Massively Parallel Genetics. Genetics. 2016;203:617-9 pubmed publisher
..These studies will help establish the disease risk of both observed and potential genetic variants and to overcome the problem of "variants of uncertain significance." ..
Shendure J, Findlay G, Snyder M. Genomic Medicine-Progress, Pitfalls, and Promise. Cell. 2019;177:45-57 pubmed publisher
Ramani V, Qiu R, Shendure J. High Sensitivity Profiling of Chromatin Structure by MNase-SSP. Cell Rep. 2019;26:2465-2476.e4 pubmed publisher
..Looking forward, we anticipate that single stranded protocol (SSP) adaptations of any protein-DNA interaction mapping technique (e.g., ChIP-exo and CUT&RUN) will enhance the information content of the resulting data. ..
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. The single-cell transcriptional landscape of mammalian organogenesis. Nature. 2019;566:496-502 pubmed publisher
..We explore the dynamics of gene expression within cell types and trajectories over time, including focused analyses of the apical ectodermal ridge, limb mesenchyme and skeletal muscle. ..
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..This framework will facilitate the large-scale mapping of enhancer-gene regulatory interactions, a critical yet largely uncharted component of the cis-regulatory landscape of the human genome. ..
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..These data define the in vivo landscape of the regulatory genome for common mammalian cell types at single-cell resolution. ..
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. Cicero Predicts cis-Regulatory DNA Interactions from Single-Cell Chromatin Accessibility Data. Mol Cell. 2018;71:858-871.e8 pubmed publisher
..Our strategy can be applied to dissect the architecture, sequence determinants, and mechanisms of cis-regulation on a genome-wide scale. ..